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Journal : Pro-Life

Kajian Pengaruh Paparan Medan Magnet terhadap Indeks Mitosis Sel Akar Bawang Putih (Allium sativum L.) Lokal Asal Timor Gede Arya Wiguna; Dicky Frengki Hanas; Welsiliana
Jurnal Pro-Life Vol. 9 No. 3 (2022): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Garlic is a horticultural plant that is used as a flavoring and also has pharmacological benefits. Local Timor Garlic, known as Eban Garlic, is one of the local varieties of garlic from Indonesia. Eban garlic is one of the leading commodities in North Central Timor Regency whose cultivation is carried out for generations. To increase the yield of garlic, the growth and development of these plants must be considered. Growth and development are part of the biological processes of organisms. In plants, primary growth occurs at the growing point, namely the meristematic tissue at the tip of the shoot and the tip of the plant root. In this article, we will examine the effect of exposure to magnetic fields on the mitotic index of Eban garlic root tip cells. The results showed that the greater the exposure to a given magnetic field, the smaller the mitotic index. The control treatment had the highest mitotic index of 36.56% and exposure to a magnetic field of 20.03 mT had the lowest mitotic index of 20.03%. The results of the study based on the time of observation, the prophase phase had the greatest mitotic index, which was 84.21% in the control treatment. This means that exposure to a magnetic field that is too high can slow down the cell division of Eban garlic roots.
Isolasi dan Uji Patogen Bakteri Asam Laktat (BAL) dari Usus Ayam Kampung yang Dipelihara Secara Intensif Di Kefamenanu Welsiliana; Charles Venirius Lisnahan; Lukas Pardosi
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This study aimed to isolate LAB from the intestines of native chickens and to test the ability of LAB activity against pathogenic bacteria. Bacterial isolation was carried out by taking chicken intestines, then diluting up to 102. The results of the dilution were taken 1 ml to be inoculated into MRSA media using the pour plate technique, streak plate technique was used to obtain pure cultures and then incubated at 37˚C for 24 hours. LAB culture testing for pathogenic bacteria was carried out using MHA media containing Escherichia coli cultures, then incubation for 120 hours at 37˚C. Observation parameters included colony shape, colony color, number of LAB colonies, the diameter of the clear zone and LAB colonies. The results showed that the bacteria isolated from the intestines of native chickens were LAB which had around colony shape and were yellowish-white (beige)-milky white. The number of LAB colonies obtained was 3.7 x 105 CFU/ml with the best inhibitory ability of pathogenic bacteria found in USA 3 isolates (1.85 cm clear zone; 0.90 cm colony size) after incubation for 120 hours.