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Imobilisasi Crude Enzyme Dari Limbah Buah dan Sayur Menggunakan Material Berbasis Cangkang Telur Retnoningtyas, Ery Susiany; Anggorowati, Adriana Anteng; Santoso, Shella Permatasari; Ayuningtyas, Debora; Juanda, Kevina Tomiko
Agro Bali : Agricultural Journal Vol 7, No 2 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i2.1729

Abstract

Berlimpahnya sisa hasil pertanian seperti kulit buah, sisa sayur dan cangkang telur berpeluang untuk diolah menjadi produk yang berharga seperti enzim maupun biomaterial. Beralihnya proses industri ke arah yang lebih ramah lingkungan berangsur-angsur memunculkan kebutuhan enzim sebagai biokatalis reaksi. Penelitian ini bertujuan untuk mempelajari potensi limbah cangkang telur sebagai material support untuk mengimobilisasi crude enzyme yang dihasilkan dari fermentasi kulit buah naga, kulit jeruk, kulit alpukat dan sisa sayur yaitu sisa batang bawah kangkung. Penelitian dilakukan dalam lingkup skala laboratorium. Metode percobaan terbagi menjadi beberapa tahapan yaitu: sintesa crude enzyme dari limbah buah dan sayur, purifikasi enzim dengan pemekatan, sintesa material berbahan dasar cangkang telur, dan tahap terakhir adalah imobilisasi crude enzyme ke dalam material. Hasil penelitian ini menunjukkan bahwa membran cangkang telur (MCT) dan olahan cangkang telur menjadi material hydroxyapatite (HA) berpotensi menjadi material untuk mengimobilisasi crude enzyme berturut-turut: 33,05 ± 3,62 U.mL-1 dan 46,73 ± 0,27 U.mL-1 untuk MCT dan HA. Dari hasil ini mengindikasikan bahwa material HA lebih berpotensi daripada MCT untuk digunakan sebagai imobilisasi enzim. Material HA dari cangkang telur yang mampu mengimobilisasi crude enzyme ini memberikan gambaran akan manfaat limbah menjadi produk yang dapat digunakan untuk pengembangan proses di industri yang konsen terhadap lingkungan.
Teknologi Tepat Guna Mesin Pencuci Porang untuk Meningkatkan Produktivitas Petani Porang di Desa Morang, Kare, Madiun: Indonesia Irawaty, Wenny; Hadi; Wijaya, Christian Julius; Indrawati, Chatarina Dian; Anggorowati, Adriana Anteng; Soetaredjo, Felycia Edi; Santoso, Shella Permatasari; Ismadji, Suryadi; Yuliana, Maria; Ernest, Edward H; Wardhani, Rinda; Afendi, Yulius; Panjaitan, Imelda
Jurnal Abdimas Madani dan Lestari (JAMALI) Volume 06, Issue 01, Maret 2024
Publisher : UII

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jamali.vol6.iss1.art9

Abstract

Recently, the productivity of porang tuber-based products has been growing well, especially after the Indonesian Government released a policy to prohibit the export of porang plants and their harvested products. One of the porang-based products is in the form of chips. Before processing, the porang tubers must be washed thoroughly to remove soil/dirt from the surface. The conventional washing process of each tuber can take up to 3-5 minutes. Therefore, the purpose of this activity is to design and manufacture porang washing equipment based on centrifuge washing model with rubber vibration. Four stages to carried out the program, i.e. designed and manufactured the washing machine, run the machine in laboratory, training activities to the porang farmers, and evaluation process. From the observation to porang farmers as our partner during the activity, it can be seen that the partners can operate the washing machine well. The results show that the washing process of porang tuber using this technology can save the washing time up to 90% compared to the conventional washing. The surface cleanliness of porang tubers resulted from the developed washing machine is the same as the conventional one. The noise level of the machine has met the requirement of Indonesian Government. It is clear that the usage of  the machine can increase the production capacity of products-based porang tuber. In addition, the use of this technology may promote a more comfortable and healthy work environment due to ergonomic reason, however, based on RULA analysis, the machine is still need to be improved in the future. Overall, the partners were satisfied with this activity and expected to have further programs in the future.