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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Harpodon Borneo Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Journal of Economic, Bussines and Accounting (COSTING) Jurnal Akuakultur Sungai dan Danau Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Mina Sains Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Buletin Jalanidhitah Sarva Jivitam Manfish Journal Policy Brief Pertanian, Kelautan, dan Biosains Tropika JARI : Jurnal Akuakultur Rawa Indonesia

Yuni Puji Hastuti

Department Of Aquaculture, Faculty Of Fisheries And Marine Science, IPB University

Author-ID : 2421151

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Library & Information Science Mechanical Engineering Social Sciences Veterinary Other

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Characteristics and Activity of Anti Qourum Sensing Bacillus spp. Isolated from Penaeus vannamei Shrimp Ponds Iman Rusmana; Yuni Puji Hastuti; Rizal Khoirun Alfisah; Alina Akhdiya
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.97-106

Certain strains of V. parahaemolyticus carry a gene that encodes a toxin that causes Acute hepatopancreatic necrosis disease (AHPND) in P. vannamei. AHPND attacks shrimp post larvae within 20-30 days after stocking causing up to 100% mortality. The expression of these virulent genes is controlled by the quorum sensing system. This system is inhibited by an anti-quorum sensing (AQS) mechanism. Several Bacillus strains have AQS mechanism by producing AHL-Lactonase enzyme. Therefore, this study aimed to obtain Bacillus spp. having AQS activity for controlling AHPND. The study was conducted from isolation and selection of Bacillus isolates, as well as determination of AQS activity. From 22 samples consisting of shrimp intestines, water and pond sediment samples, a total of 151 isolates of Bacillus spp. were isolated. The screening test for AQS activity obtained 11 isolates that showed AQS activity on Cromobacterium violaceum. Determination of violacein pigment in liquid cultures of C. violaceum showed the index value of the pigment formation was between 0.025-0.166 and 0.026-0.567 at 24-hour and between 48-hour incubations, respectively. The quantitative analysis of violacein production showed that there were six isolates of Bacillus could inhibit the pigment production more than 75%. The isolates were identified as Bacillus cereus (four isolates), Bacillus thuringiensis (one isolate), and Bacillus velezensis (one isolate), respectively. The molecular analysis had confirmed that the isolates have aiiA genes encoding AHL-lactonase enzyme. These Bacillus isolates have potential application for controlling AHNPD disease.

SUHU TERBAIK UNTUK MENINGKATKAN PERTUMBUHAN DAN KELANGSUNGAN HIDUP BENIH KEPITING BAKAU Scylla serrata DI SISTEM RESIRKULASI Yuni Puji Hastuti; Ridwan Affandi; Radhita Millaty; Wildan Nurussalam; Siska Tridesianti
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 11 No. 2 (2019): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

One of the abiotic factors that affects the growth and the survival of mud crabs is temperature. The optimum temperature media will result in increasing of growth rate and survival rate on mud crabs Scylla serrata because it is related to the metabolism process. This study aimed to examine the effect of temperature on the survival rate (SR) and spesific growth rate (SGR) of mud crab through the reaction of physiological condition. This study consisted of the treatments with the temperature of 25 °C (A), the temperature of 27 °C (B), the temperature of 29 °C (C), dan the temperature of 31 °C (D). Based on the research result obtained 29 °C is the best temperature for the maintenance of mud crab with recirculation system, this can be seen from the result of feed conversion ratio, specific growth and survival of mud crab that have the best than other treatments.

PENAMBAHAN SHELTER UNTUK PENGENDALIAN RESPONS FISIOLOGI DAN PRODUKSI KEPITING BAKAU Scylla serrata PADA SISTEM RESIRKULASI AKUAKULTUR Yuni Puji Hastuti; Priyo Handoyo Wicaksono; Wildan Nurusallam; Siska Tridesianti; Yuli Siti Fatma; Kukuh Nirmala; Iman Rusmana; Ridwan Affandi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 12 No. 1 (2020): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Ketersediaan shelter (tempat perlindungan) pada kepadatan spesifik diharapkan dapat meningkatkan produksi kepiting bakau (Scylla serrata) dalam sistem resirkulasi. Shelter sebagai salah satu faktor abiotik berperan penting dalam mengurangi kematian yang disebabkan oleh kanibalisme kepiting dan menekan tingkat stres biota. Sistem Resirkulasi Akuakultur (RAS) dengan bak kotak kultur berkapasitas 60 L yang didukung oleh shelter diperkirakan menghasilkan respons fisiologis dan pertumbuhan kepiting bakau yang paling baik. Penelitian ini bertujuan untuk mengevaluasi dampak penambahan shelter pada lingkungan dengan sistem resirkulasi terhadap respons fisiologis dan produksi kepiting bakau S. serrata dengan kepadatan 10 kepiting per satu bak kultur yang berisi 60 L air laut. Penelitian ini dilakukan dengan tiga perlakuan penambahan shelter, yaitu 2 shelter (S2), 4 shelter (S4), 6 shelter (S6), dan kontrol tanpa shelter (C). S6 adalah perlakuan terbaik dengan tingkat kelangsungan hidup 73,33±5,8%, laju pertumbuhan spesifik 0,886±0,014%, pertumbuhan lebar karapas 0,024±0,004 cm/hari, dan rasio konversi pakan terendah dibandingkan dengan perlakuan lain. Perlakuan S6 secara signifikan mempengaruhi jumlah hemosit total kepiting pada awal budidaya (P <0,05). Penambahan 6 shelter dapat mengoptimalkan pertumbuhan kepiting lumpur dengan kepadatan 10 kepiting dalam satu bak kotak kultur.

EVALUASI PENGGUNAAN PASIR MALANG SEBAGAI BAHAN FILTER TERHADAP KUALITAS AIR, RESPON FISIOLOGI DAN PRODUKSI KEPITING BAKAU Scylla serrata Yuni Puji Hastuti; Arul Tabah Prastomo; Ridwan Affandi; Wildan Nurussalam; Dudi Muhammad Wildan; Syamsul Bahri Agus
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 12 No. 3 (2020): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v12i3.32939

Mud crab Scylla serrata is one of crustacean commodities with high economic value. One of the unsolved obstacles in mud crabs cultivation is crabs’ stress level caused by the declining water quality. The recirculating system is a method for maintaining water quality throughout the rearing period of culture crab. This system can use a variety of physical filter materials, such as zeolite, sand, and other materials. This study aimed to determine the amount of malang sand as a physical filter which positively affect the physiological responses and production performance of mud crabs. This research was carried out using a recirculating system with a completely randomized design conducted in five treatments with three replicates, i.e., malang sand with a weight of 0 kg (control), 5 kg, 10 kg, 15 kg, and 20 kg. The results proved that the best physiological response and production performance of mud crab was observed at malang sand treatment with a weight of 5 kg. This treatment showed specific growth rate, absolute growth rate for body weight, absolute growth rate for body length, and the survival rate of mud crabs with the highest value of 0.18±0.061%, 0.13±0.05 g/day, 0.0016±0.00006 cm/day, and 77.77%, respectively, yet it had the lowest feed conversion ratio of 3.76±004. In addition, the 5 kg treatment had water quality parameters in the tolerable ranges for mud crabs’ growth.

Kebiasaan Makanan Belangkas, Tachypleus gigas (Müller 1785) dan Carcinoscorpius rotundicauda (Latreille 1802) di Perairan Pesisir Balikpapan, Kalimantan Timur Rani Nuraisah; Naila K Aini; Ali Mashar; Zairion Zairion; Yuni P Hastuti; Peter Funch; Yusli Wardiatno
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol. 10 No. 2 (2020): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.10.2.153-162

Horseshoe crabs are living fossils found in Indonesia. This study examined the food habit of horseshoe crabs (Carcinoscorpius rotundicauda and Tachypleus gigas) collected from Balikpapan coastal waters, East Kalimantan. Horseshoe crabs were captured using gill nets and picked by hand along the Balikpapan coast. The width of the prosoma and the body weight of each individual were measured, and the gut contents were analysed to determine the preponderance index, food niche breadth, and niche overlap. Carcinoscorpius rotundicauda prosoma widths ranged from 4.1 to 15.4 cm and those of Tachypleus gigas ranged from 2.8 to 24 cm. Eight food items were found in the gut of the horseshoe crabs, namely: bivalves, gastropods, scaphopoda, polychaetes, echinoderms, crustaceans, leaf litter and others. Based on the preponderance index, gastropods were the main food item of the two horseshoe crab species. In accordance to the food composition the horseshoe crabs inhabiting Balikpapan coastal waters are categorized as benthivores.

The evaluation of turmeric (Curcuma longa) supplementation within feed as an antioxidant towards growth performance of catfish Clarias gariepinus Burchell 1822 in zero water exchange condition Thoy Batun Citra Rahmadani; Dedi Jusadi; Mia Setiawati; Yuni Puji Hastuti
Jurnal Iktiologi Indonesia Vol 20 No 2 (2020): June 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i2.518

The objective of this research was to evaluate the supplementation of turmeric in the diet on antioxidant status and growth performance of African catfish (Clarias gariepinus) in zero water exchange condition. This study used a completely randomized design with 4 treatments and 4 replications. Each treatment consisted of feed supplementation turmeric at dosage of 0; 2.5; 5 or 7.5 g kg-1 diet. One hundred catfish juvenile (5.95±0.05 g) were stocked in intermediate bulk container (IBC) tank (1×1×1 m3) and rearing in zero water exchange condition for 60 days. Catfish were fed at satiation twice a day, in the morning and evening. The results showed that an increase in antioxidant content in catfish fed with the addition of turmeric, which simultaneously also reduced the percentage of liver damage. The parameters of liver damage can be seen from several parameters i.e. pale liver, droplet fat and fat content in the addition of turmeric treatment is lower than without the addition of turmeric. However, catfish fed with the addition of turmeric did not show significant results in terms of growth performances. Abstrak Tujuan penelitian ini adalah untuk mengevaluasi penambahan kunyit ke dalam pakan sebagai antioksidan dan kinerja pertumbuhan ikan lele Afrika (Clarias gariepinus). Penelitian ini menggunakan rancangan acak lengkap dengan 4 perlakuan dan 4 ulangan. Setiap perlakuan terdiri atas penambahan dosis kunyit sebanyak 0; 2,5; 5 dan 7,5 g kg-1 pakan. Seratus benih ikan lele (5,95±0,05 g) dipelihara dalam tangki Intermediate Bulk Container (IBC) (1×1×1 m3) dan dipelihara tanpa pergantian air selama 60 hari. Ikan lele diberi pakan secara at satiation dua kali sehari. Hasil penelitian menunjukkan bahwa terjadi peningkatan kandungan antioksidan pada ikan lele yang diberi pakan dengan penambahan kunyit di dalamnya, yang secara bersamaan juga mengurangi persentase kerusakan hati. Parameter kerusakan hati dapat dilihat dari beberapa parameter, seperti hati pucat, droplet lemak dan kandungan lemak pada perlakuan penambahan kunyit lebih rendah dibandingkan tanpa penambahan kunyit. Namun ikan lele yang diberikan pakan dengan penambahan kunyit tidak menunjukkan hasil yang signifikan dari segi pertumbuhannya.

The color quality of Sumatra barb Puntigrus tetrazona (Bleeker, 1855) in different light spectrum exposure Wijianto Wijianto; Kukuh Nirmala; Yuni Puji Hastuti; Eddy Supriyono
Jurnal Iktiologi Indonesia Vol 20 No 3 (2020): October 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i3.534

The color quality of Sumatra barb that cultivated by the farmers are not as good as the fish that collected from the wild. One of the causes is the unsuitable environment for maintaining and breeding Sumatran barb. This research aims to compare color quality of Sumatra barb Puntigrus tetrazona by exposure the different light spectrums on maintenance media. The experiment was completely randomize design with six treatments and three replications consisted of K (control), R (room light), M (red light spectrum), H (green light spectrum), B (blue light spectrum) and P (white light spectrum). The method used to measure Sumatra barb color quality using Photoshop CS 5 software and chromatophore cell calculations. The results of color quality analysis after 28 showed that the (M) treatment had the highest percentage of color quality was 48.81 ± 1.57% for orange color and 32.26 ± 0.07% for black color. The highest number of chromatophore cells was in M treatment with 147 ± 3.7 cells mm-². The red light spectrum (M) treatment showed the best physiological response and improvement of color quality and the glucose level was 23.00 ± 1.00 mg dL−1. The best color quality of the Sumatra barb is produced by exposure to the red light spectrum (M). Abstrak Ikan sumatra hasil budi daya petani belum mencapai kualitas warna yang cukup baik dibandingkan hasil tangkapan di alam. Salah satu penyebabnya adalah lingkungan pemeliharaan serta penangkaran ikan sumatra yang tidak sesuai. Penelitian ini bertujuan untuk mengevaluasi kualitas warna ikan sumatra Puntigrus tetrazona dengan paparan spek-trum cahaya berbeda pada media pemeliharaan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas enam perlakuan dan tiga ulangan yaitu kontrol (K), cahaya ruang (R), spektrum cahaya merah (M), spek-trum cahaya putih (P), spektrum cahaya biru (B), dan spektrum cahaya hijau (H). Metode yang digunakan untuk mengukur kualitas warna ikan sumatra yaitu perangkat lunak Photoshop CS 5 dan perhitungan sel kromatofora. Hasil analisis kualitas warna setelah 28 hari menggunakan perangkat lunak Photoshop CS5 menunjukkan perlakuan spek-trum cahaya merah (M) memiliki kualitas warna terbaik dengan persentase sebesar 48,81±1,57% untuk warna jingga dan 32,26±0,07% untuk warna hitam. Jumlah sel kromatofora tertinggi yaitu pada perlakuan spektrum cahaya merah (M) yaitu sebesar 147,3±3,7 sel mm-2. Spektrum cahaya merah (M) menunjukkan respons fisiologis dan peningkatan kualitas warna yang terbaik. Kadar glukosa pada perlakuan spektrum cahaya merah (M) yaitu sebesar 23,00±1,00 mg dL-1. Kualitas warna ikan sumatra Puntigrus tetrazona terbaik dihasilkan oleh paparan spektrum cahaya merah (M).

Color quality, behavioral response, and blood glucose levels of guppies Poecilia reticulata (Peters, 1859) with the addition of Indian almond leaves (Terminalia catappa) in fish containers Izhar Amirul Haq; Kukuh Nirmala; Yuni Puji Hastuti; Eddy Supriyono
Jurnal Iktiologi Indonesia Vol 22 No 1 (2022): February 2022
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v22i1.581

Guppies are ornamental fish that have economic value and can be cultivated. One of the problems in guppies cultivation is the poor quality of body color. The solution to this problem is to improve the environmental quality in guppy aquaculture. This study evaluates changes in color quality, behavioral response, and blood glucose levels of guppies using water immersion of Indian almond leaves on rearing media. Study was carried out using a completely randomized design consisting of five treatments. The rearing media was filled water with a total volume of 10 L from each treatment with different concentrations, namely, Control treatment (100% freshwater), A (75% freshwater + 25% Indian almond leaf water), B (50% freshwater + 50% Indian almond leaf), C (25% freshwater + 75% Indian almond leaf) and D (100% Indian almond leaf). Each treatment observed several test parameters such as color quality percentage, survival rate, chromatophores cells number, behavior, glucose levels, and the water physical-chemical parameters. Guppies' behavioral responses, in general, experienced an increasing change in each treatment for seven days. The highest color quality percentage by treatment D was 73.93±2.29%, and the highest glucose level was by the control treatment, which was 24.11±0.41 mg dL-1. The results of variance analysis showed that the water immersion of Indian almond leaves was significantly different on color quality, behavioral response, and blood glucose levels through Duncan's test (p<0.05). Abstrak Ikan guppy termasuk ikan hias yang memiliki nilai ekonomis dan dapat dibudidayakan. Salah satu masalah dalam budidaya ikan guppy yaitu kualitas warna tubuhnya yang tidak bagus. Upaya yang dapat dilakukan yaitu memperbaiki lingkungan budidaya ikan guppy. Studi ini bertujuan untuk mengevaluasi perubahan kualitas warna, respons tingkah laku, dan kadar glukosa ikan guppy menggunakan rendaman air daun ketapang pada media pemeliharaan. Evaluasi ini dilakukan dengan menggunakan rancangan acak lengkap yang terdiri atas lima perlakuan. Media pemeliharaan diisi air dengan total volume 10 L dari setiap perlakuan dengan konsentrasi yang berbeda yaitu, perlakuan Kontrol (100% air tawar), A (75% air tawar + 25% air daun ketapang), B (50% air tawar + 50% air daun ketapang), C (25% air tawar + 75% air daun ketapang) dan D (100% air daun ketapang). Setiap perlakuan diamati beberapa parameter uji seperti persentase kualitas warna, tingkat sintasan, jumlah sel kromatofora, tingkah laku, kadar glukosa, dan parameter fisik kimiawi perairan. Respons tingkah laku ikan guppy secara umum mengalami perubahan yang meningkat dalam setiap perlakuan selama tujuh hari. Persentase kualitas warna tertinggi pada perlakuan D yaitu sebesar 73,93±2,29% dan nilai kadar glukosa tertinggi pada perlakuan Kontrol yaitu 24,11±0,41 mg dL-1. Hasil analisis sidik ragam menunjukkan bahwa rendaman air daun ketapang berbeda nyata terhadap kualitas warna, respons tingkah laku dan kadar glukosa darah melalui uji Duncan (p0,05).

IMMUNE RELATED GENES EXPRESSION ANALYSIS IN KOI FISH AFTER VACCINATED WITH KOI HERPES VIRUS DNA VACCINES Sri Nuryati; Fauzan Wahib Alsani; Hasan Nasrullah; Odang Carman; Yuni Puji Hastuti; Eni Kusrini; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 15, No 1 (2020): (June, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.1.2020.25-32

Vaccination is a practical step in preventing diseases caused by koi herpes virus (KHV) in koi fish (Cyprinus carpio haematopterus). We have developed two DNA vaccines for KHV named as GP-25 and GP-11 from two local isolates coded as ORF25 and ORF81, respectively. Although both vaccines have been reported to increase survival rates, the evaluation of koi fish immune responses at the molecular level has not been done post-vaccinations. The aim of this research was to determine the effects of koi herpesvirus DNA vaccine on the immune-modulation of koi fish at mRNA level. This recent research used the best vaccine doses of both vaccines determined from our previous study: 7.5 and 12.5 µg per 100 g fish of GP-11, and 12.5 µg per 100 g fish of GP-25. The immune gene expression was analyzed using the RT-qPCR method from the fish liver at 0, 1, 7, 14, and 28 days post-vaccination (dpv). The results showed that, in the vaccinated fish, the immune genes viz. tumor necrosis factor a (TNFa), interleukin-1b (IL1b), interferon-g (IFNg), Mx1, immunoglobulin Mu chain (IgM), and major histocompatibility complex (MHC) class I and class II were induced to significant extents. The higher dose vaccination using the GP-11 vaccine showed higher immune gene expression than that of the lower dose. Furthermore, the GP-25 vaccine had induced lower immune responses than the GP-11 vaccine when using the same dose of vaccination, but relatively the same when the half-dose of GP-11 vaccine was used. In conclusion, the GP-11 and GP-25 vaccine provided the immune-modulatory effects on the koi fish immune response after vaccination.

PEMANFAATAN PERIFITON PADA JUMLAH SUBSTRAT BERBEDA TERHADAP KUALITAS AIR DAN KINERJA PERTUMBUHAN BENIH IKAN GURAME (Osphronemus gouramy) Atma Jaya Salman Muin; Kukuh Nirmala; Mia Setiawati; Yuni Puji Hastuti
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Tujuan penelitian ini adalah untuk menganalisis pemanfaatan jumlah substrat tali rafia yang berisi perifiton dalam meningkatkan kualitas air media pemeliharaan untuk pertumbuhan benih ikan gurame (Osphronemus gouramy). Perlakuan terdiri dari penambahan A (0 substrat), B (1 substrat), C (2 substrat), dan (3 substrat) tali rafia berisi perifiton (bobot 5 g/substrat). Benih ikan gurame 32 ekor (panjang total 4,8±0,30 cm dan bobot 1,9±0,38 g/ekor) dipelihara pada akuarium ukuran 27 cm x 30 cm x 60 cm. Selama pemeliharaan benih ikan gurame diberi pakan komersil secara at satiation tiga kali sehari. Parameter yang diamati adalah kualitas air, laju pertumbuhan spesifk, tingkat kelangsungan hidup, dan rasio konversi pakan. Hasil analisis kualitas air diperoleh nilai kisaran optimum untuk pertumbuhan benih ikan gurami pada perlakuan C (2 substrat) dan perlakuan D (3 substrat), Hasil penelitian menunjukkan bahwa terdapat pengaruh nyata (P<0,05) pada penambahan substrat yang berbeda terhadap kinerja pertumbuhan benih ikan gurame. Pada perlakuan C (2 substrat) memiliki performa pertumbuhan terbaik yaitu laju pertumbuhan spesifik panjang (cm) 1,33±0,03, laju pertumbuhan spesifik bobot (g) 4,03±0,12, tingkat kelangsungan hidup (%) 86,46±1,04, dan rasio konversi pakan 0,81±0,01. Substrat tali rafia dapat dimanfaatkan sebagai media menempelnya perifiton, pemanfaatan 2 substrat tali rafia (10 gr) dapat diperoleh beberapa kelas perifiton yang dapat dijadikan sumber pakan alami untuk meningkatkan performa pertumbuhan benih ikan gurame dan dapat mempertahankan kualitas air media pemeliharaan. Perlu dilakukan kajian lebih lanjut mengenai pemanfaatan jenis substrat lainnya untuk meningkatkan produksi perifiton agar mengurangi penggunaan pakan komersil.The purpose of this study was to determine the optimum number of filled-periphyton raffia substrates serving as natural food to improve the growth performance of gouramy seeds (Osphronemus gouramy) and maintain the water quality rearing media. The treatments consisted of the addition of A (0 substrate), B (1 substrate), C (2 substrates), and D (3 substrates) raffia rope substrates filled with periphyton (5 g periphyton/substrate). Gouramy seeds of 32 individuals (total length 4.8±0.30 cm dan weight 1.9±0.38 g/ind.) were reared in an aquarium measuring 37 cm x 30 cm x 60 cm in size. During the rearing period, the gouramy seeds were fed with a commercial diet at satiation three times a day. The parameters observed were water quality parameters, specific growth rate, survival rate, and food conversion ratio. The results showed that treatment C (2 substrates) and D (3 substrates) had the optimum ranges of water quality to support the growth of gourami seeds. The statistical analysis also confirmed a significant (P<0.05) effect of the addition of different substrates to the growth performance of gouramy seeds. Gouramy seeds in treatment C (2 substrates) had the best growth performance in terms of specific length growth rate (cm) 1.33±0.03, specific weights growth rate (g) 4.03±0.12, survival rate (%) 86.46±1.04, and food conversion ratio 0.81±0.01. The raffia rope substrate can be used as a medium for attaching periphyton. The use of 2 raffia rope substrates (10 g) can be obtained from several classes of periphyton which can be used as a natural food source to improve the growth performance of gourami seeds and maintain the water quality of the maintenance media. It is necessary to carry out further studies regarding the use of other types of substrates to increase periphyton production in order to reduce the use of commercial feeds.

Co-Authors . Zairion Adianto, Asep Ali Mashar Alimuddin Alina Akhdiya Anwar, Rifky Alwafi Arul Tabah Prastomo Atma Jaya Salman Muin Bianingrum Bianingrum Chandra Yudistira, Chandra Daniel Djokosetiyanto DEDI JUSADI Dudi Muhammad Wildan Eddy Supriyono Eni Kusrini Fauzan Wahib Alsani Haris luthfi Hasan Nasrullah Hutagaol, Manuntun Paruliah I Gede Made Wirabrata I. Effendi Ide Permatasari Iman Rusmana Iman Sari Lubis, Vina Irzal Effendi Izhar Amirul Haq K. Nirmala Kosasih Kosasih Kukuh Nirmala Kuntari, Wahyu Budi Kurnia Faturochman, Kurnia Kurnia Faturrohman Kurniawan, Ahmad Rahardi Lena Novita Lesmana, Dudi Mafatih Devi Safrina, Mafatih Devi Malau, Albert Gamot Melati, Aulia Firda Mia Setiawati Moh. Burhanuddin Mahmud Muhammad Muhammad N Hutomo Nadeak, Horas Naila K Aini Nirmala , Kukuh Nurmawati, Subekti Odang Carman Orbita Roiyan Dhuha, Orbita Roiyan Peter Funch Pras, Eva Prasetiyono Priyo Handoyo Wicaksono Radhita Millaty Rani Nuraisah Ridwan Affandi RIDWAN AFFANDI Rina Hesti Utami Rizal Khoirun Alfisah Setyo Pertiwi Siska Tridesianti Sri Nuryati Sukenda . Sukma Dhani Syamsul Bahri Agus, Syamsul Bahri T. Widiyanto Tanjung, Tanjung Thoy Batun Citra Rahmadani Tri Widiyanto Tridesianti, Siska Tyas Setioaji Vika Yuniar Wardani, Sulistia Wijianto Wijianto Wijianto Wijianto Wildan Nurusallam Wildan Nurusallam, Wildan Wildan Nurussalam Yuli Siti Fatma Yuli Siti Fatma Yusli Wardiatno

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