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Roosje Rosita Oewen, Roosje Rosita
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The relation between enamel defects in deciduous teeth, nutritional status and the development of basic skills in children with small for gestational age Miguna, Katina; Soewondo, Williyanti; Oewen, Roosje Rosita; Fadiyana, Eddy
Padjadjaran Journal of Dentistry Vol 24, No 3 (2012): November 2012
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol24no3.26834

Abstract

Introduction:  Small for Gestational Age (SGA)  babies  are those whose birth weight are less than 2 SD or below the 10th percentile curve of Lubchenco intrauterine growth and development. The cause of SGA is due to an Intra-uterine growth restriction (IUGR).  IUGR inhibits the development of various organs, including the enamel of deciduous teeth and the central nervous system (CNS). The research aims to analyzed correlation between enamel defects in deciduous teeth, nutritional status and the development of basic skills in children with SGA. Methods: A total of 28  SGA children aged between 3-6 years were obtained from Hasan Sadikin Hospital. The sample comprised of 20 boys and 8 girls to be studied for tooth enamel defects in deciduous teeth, weight, height and development basic capabilities. Study design was descriptive and analytical, with cross-sectional study. Scoring modification Developmental Defect of Enamel (DDE) of the FDI was used to assess the enamel defects in deciduous teeth. Enamel Defect Score (EDS) was calculated according to the Willyanti’s formula. The nutritional status was measured by measuring height, weight, and age and converted to the diagram of  Body Mass Index (BMI) / Age of the WHO Child Growth Standards. While the development of SGA children basic skills was measured using pre- screening questionnaire development (KPSP). To determine the linkage between enamel defects  in deciduous teeth and nutritional status with basic skills development in SGA children, the non-parametric statistical analysis of Kendall’s Coefficient of Concordance was performed. Results: Statistical test results showed that Kendall’s Coefficient of Concordance (W) = 1.00 is statistically significant with Chi-square value = 56.00 with p (value) <0.01. Conclusion: There is a significant relationship between EDS and nutritional status with the SGA children development of basic skills as well as the development of the child’s ability.
The comparison of deciduous teeth eruption pattern in small gestational age (SGA) and appropriate gestational age (AGA) children Kirana, Irawati; Oewen, Roosje Rosita; Soewondo, Williyanti
Padjadjaran Journal of Dentistry Vol 22, No 2 (2010): July 2010
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol22no2.26850

Abstract

Introduction: Small Gestational Age (SGA) children is defined as infant whose birth’s weight was below the tenth percentile intrauterine growth and development curve of Lubchenco. There are two types of SGA children, namely symmetrycal SGA (the disturbance occurred in the first trimester of pregnancy) and asymmetrical SGA (the disturbance occured in second and third trimesters of pregnancy). The aim of this research was to obtain the deciduous teeth eruption patterns in symmetrical and asymmetrical of SGA children, based on baby teeth that  have erupted the eruption of primary teeth. Methods: The research design was descriptive and analytical, with cross-sectional design and using the given sample size. The sample were 28 SGA children aged 1-4 years and 33 Appropriate Gestational Age (AGA) children. Determination of SGA children based on Ponderal Index. Eruptive stage was determined by Nola Modification Scoring by the score between 0-4. (Score (0) for tooth that have not erupted; (1) tooth emerge, (2) ⅓ crown  erupted tooth, (3) ½ crown erupted tooth, (4) full crown tooth. Results: The results showed, that there were differences in the pattern of deciduous teeth eruption based on teeth eruption stages that have been erupted. Eruption pattern of SGA children deciduous teeth were slower than the eruption pattern of AGA children deciduous teeth. Conclusion: Different patterns of deciduous teeth eruption in symmetrical SGA children and asymmetrical SGA children were showed. Viewed from the stand point of view the stage of teeth eruption, the deciduous teeth eruption pattern of symmetrical SGA children was slower than the asymmetrical SGA children.
Influence of surface modification to stainless steel on initial Streptococcus mutans adhesion and antibacterial acivity Nurhaerani, Nurhaerani; Oewen, Roosje Rosita; Herdiyati, Yetty
Padjadjaran Journal of Dentistry Vol 22, No 2 (2010): July 2010
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol22no2.26846

Abstract

Introduction: Stainless steel is one of the most frequently used material in pediatric dental clinic for stainless steel crown, bracket, molar band and archwire. The purposes of this study were to evaluate the surface characteristic (chemichal composition, hydrophobicity, fluoride release), antibacterial activity of modified stainless steel and to evaluate the initial adhesion S. mutans on the surface of modified stainless steel. Methods: This study was an experimental laboratory. Sample used in this study was Stainless steel which modified with the plasma based fluorine ion implantation. The stainless steel which was fluorine ion implanted by CF4 gas was washed in a ultrasonic bath containing distilled water for 10 minute. The modified stainless steel materials were washed in an ultrasonic bath containing distilled water for 10 minute. Stainless steel material was incubated at 37°C in 2 ml of BHI broth containing S. mutans with concentration 2x107 CFU/ml. After 48 hours incubation, a 0.5 ml of solution was immediately transferred into 4.5 ml of PBS (-) and diluted. A 100 pi of diluted solution was plated on BHI agar. After 48 hours culture at 37°C, the number of colonies was counted. The numbers of fluorine ion implanted material and fluorine ion non-implanted control were ten, respectively. Results: XPS analysis showed the presence of fluorine and chromic fluoride until second layer. The contact angle of modified stainless steel was significantly increased A small amount of fluorine ion released from surface of modified stainless steel was detected until the second day. Fluorine ion implanted of stainless steel was significantly decreased the initial adhesion and increased the antibacterial activity. Conclusion: The adhesion of S. mutans on the surface of stainless steel for 4 hours incubation is shown in Figure 11. Fluorine ion implanted stainless steel showed less bacterial adhesion than fluorine ion non- implanted control (p<0.001).
The effect of ethanol extract of mangostene (Garcinia Mangostana Linn.) peel on tongue cancer cells Supri's Clone-1 apoptosis, in vitro Titiana, Maria Aurora; Oewen, Roosje Rosita; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November 2011
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1045.519 KB) | DOI: 10.24198/pjd.vol23no3.14029

Abstract

Apoptosis or programmed cell death serves to regulate physiological and pathological conditions. Garcinia mangostana Linn. is one of the medical herbs that is widely used to suppress human tongue cancer growth by inducing apoptosis. The research aimed to analyze the effect of ethanol extract of Garcinia mangostana Linn. on tongue cancer cells apoptosis Supri's Clone-1 (SP-C1). The type of the research was an experimental laboratory. Tongue cancer cells SP-C1 were treated by various ethanol extract concentrations (0, 300, 400, 500, 600, 700 pg/ml) of Garcinia mangostana Linn. to detect the apoptosis, which was done by acridine orange and ethidium bromide coloring tests. The number of tongue cancer cells SP-C1 2x104 cells/dish. Observation on cells apoptosis was observed by a fluorescent microscope with 40 x magnification. The data were analyzed using one-way ANOVA and was followed by Post Hoc test (Tukey-test) with 95% significance level. The result showed that tongue cancer cells SP-C1 apoptosis treated by various ethanol extracts of Garcinia mangostana Linn. increase significantly. The highest effect of apoptosis was detected at the 700 pg/ml concentration that has an effect on the percentage of tongue cancer cells apoptosis by 65%.
Ethanol extract of mangosteen (Garcinia Mangostana Linn) peel effect in inhibiting the growth of human tongue cancer cells Supri’s Clone 1, invitro Suanto, Edi; Oewen, Roosje Rosita; Sasmita, Inne Suherna; Supriatno, S.; Supratman, Unang
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.616 KB) | DOI: 10.24198/pjd.vol23no2.14022

Abstract

The incidence of tongue cancer in Indonesia reached 1.01% of all cancers and 42% of oral cavity cancer. Tongue cancer therapies including chemotherapy, radiotherapy, surgery, and all three combined therapy. Search for anti-cancer drugs currently switched on herbal plants, one of which is the mangosteen. Has the properties of mangosteen peel extract inhibited the growth of cancer cells. The purpose of the study, obtain IC50 of ethanol extract of mangosteen peel in inhibiting the growth of human tongue cancer cells SP-C1. Research carried out on 96 preparations of human tongue cancer SP-C1 were incubated with ethanol extract of mangosteen peel, preparations were classified in two groups of incubation time (24 hours and 48 hours) and each group will be given preferential treatment over 6 randomly different concentrations: 0 (control), 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL and 1000 μg/mL. Model experiments were 2 x 6 factorial experiment with eight replication for each cell. Test results with ANAVA, incubation (24 and 48 hour) SP-tongue cancer cells with various concentrations of C1 ethanol extract of mangosteen peel gives a highly significant, indicating differences cancer cell growth inhibition. Incubation time factor showed the long incubation effect on cancer cell growth inhibition. Furthermore, by Newman Keuls test, showed 500μg/mL concentrations of 24-hour incubation had the best effect. Conclusion of the study of ethanol extract of mangosteen peel could achieve with IC50 values of cell growth resistance 50.3% at a concentration of 500 μg/mL and an incubation time of 24 hours.
Co-Authors Achmad Syawqie Anak Agung Gede Sugianthara Arlette Suzy Puspa Pertiwi Dian Anggraeni Edi Suanto, Edi Eriska Riyanti Fadiyana, Eddy Harun Achmad, Harun Henri Hartman, Henri Inne Suherna Sasmita Jakobus Runkat, Jakobus Kania, Annisa Maya Kania, Annisa Maya Kirana, Irawati Maria Aurora Titiana, Maria Aurora Maureen Martina, Maureen Mieke Hemiawati Satari Miguna, Katina Nurhaerani, Nurhaerani Soewondo, Williyanti Supriatno Suwondo, Williyanti Suwondo, Williyanti Syarief Hidayat Unang Supratman Yetty Herdiyati, Yetty