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All Journal Jurnal Katalisator PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JURNAL KESEHATAN PERINTIS Jurnal Farmasi Higea Jurnal Ilmiah Farmasi Farmasyifa Indonesia Natural Research Pharmaceutical Journal (INRPJ) Jurnal Pengabdian Masyarakat (ABDIRA) Jurnal Katalisator FASKES : Jurnal Farmasi, Kesehatan, dan Sains
Verawati
Universitas Perintis Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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STABILITAS ANTOSIANIN DARI KULIT TERONG BELANDA MERAH (Solanum betaceum Cav.) TERHADAP PH DAN SUHU Sandra Tri Juli Fendri; Verawati Verawati; Putri Saimi Nuras
Jurnal Katalisator Vol 5, No 1 (2020): KATALISATOR
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22216/jk.v5i1.5023

Abstract

There had been identification and stability test of anthocyanin liquid extract from skin of eggplant of Holland that red colored using UV-Vis spectrophotometer. Anthocyanin is a color compound that responsible mostly red, blue and purple in fruit, vegetables and ornamental plants and these anthocyanins belong to the flavonoid group. Eggplans of Holland is taken from Tabing market, Padang City. The extraction process was performed by maceration method using solvent of 96% ethanol: HCl 1% (9:1). The identification of the anthocyanin compound on the sample extract was done by color reaction with 2 M NaOH and 2 M HCl. Stability of the anthocyanin pigment was tested by both the pH and temperature difference treatments. The pH used was pH 1, 3, 5, 7 and 9, while the temperature used was 30oC, 40oC, 60oC, 80oC and 100oC. The parameters of stability are the color of solution, maximum wavelength (λmax) and absorbance. The anthocyanin pigments stability test Eggplant of Holland was performed by using one-way of statistical data of variances (ANOVA), the liquid leather extract was stated stable at the influence of pH to temperature (temperature 30, 40, 60 and 80oC), especially at pH 1-3 next pH 5 and pH 7.Keywords : Anthocyanin, identification, pH, temperature, stability test
Profil Kimia dan Aktivitas Antioksidan Fraksi Air Terhidrolisis dari Daun Piladang (Solenostemon Scutellarioides) Verawati Verawati; Dira Dira; Dira Arieska
Jurnal Katalisator Vol 4, No 1 (2019): KATALISATOR
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (748.46 KB) | DOI: 10.22216/jk.v4i1.3892

Abstract

Daun Piladang (Solenostemon scutellarioides) merupakan obat tradisional Sumatera Barat yang memiliki banyak kandungan metabolit sekunder berpotensi sebagai antioksidan. Metabolit sekunder seperti golongan fenolat yang terdapat pada fraksi polar umumnya terdapat dalam bentuk glikosida. Proses hidrolisis dapat memutuskan ikatan gula sehingga dapat melepaskan aglikon yang memiliki aktivitas antioksidan lebih tinggi daripada bentuk glikosidanya. Pada penelitian ini dilakukan hidrolisis asam terhadap fraksi air (polar) daun piladang, menggunakan asam asetat, asam fosfat dan asam klorida. Profil kimia fraksi hasil hidrolisis ditentukan secara kualitatif dengan KLT dan secara kuantitatif dengan metode Folin Ciocalteu untuk memperoleh kadar fenolat total.  Aktivitas antioksidan fraksi hasil hidrolisa diperiksa dengan metode perangkapan radikal DPPH. Hasil penelitian menunjukkan adanya perbedaan profil KLT dari masing-masing fraksi hasil hidrolisis. Kadar fenolat tertinggi diperoleh pada hidrolisis dengan asam fosfat yaitu 7,736 % diikuti asam klorida 4,218% dan asam asetat 2,024%. Aktivitas antioksidan tertinggi ditunjukkan pula oleh hasil hidrolisis dengan asam fosfat yaitu dengan IC50 413,92 ppm yang termasuk ke dalam kategori lemah.Piladang leaves (Solenostemon scutellarioides) is a traditional West Sumatra medicine which has a lot of secondary metabolite content which has the potential as an antioxidant. Secondary metabolites such as the phenolic group found in the polar fraction are generally in the form of glycosides. The hydrolysis process can break the bond of sugar so that it can release aglycone which has higher antioxidant activity than the form of glycosides. In this study,  the acid hydrolysis of the water fraction (polar) of piladang leaves was carried out using acetic acid, phosphoric acid and hydrochloric acid. The chemical profile of the hydrolysis fraction was determined qualitatively by TLC and quantitatively by the Folin Ciocalteu method to obtain total phenolic levels. The antioxidant activity of the hydrolysis fraction was examined by the DPPH radical scavenging method. The results showed a difference in the TLC profile of each hydrolysis fraction. The highest phenolic content was obtained by hydrolysis with phosphoric acid which was 7,736% followed by hydrochloric acid 4,218% and acetic acid 2,024%. The highest antioxidant activity was also shown by the results of hydrolysis with phosphoric acid, with IC50 413.92 ppm which was included in the weak category.
Identifikasi Molekular Bakteri Endofit dari Akar Pauh (Mangifera sumatrana Miq) dan Aktivitas Antimikrobanya Zaunit, Muthia Miranda; Verawati, Verawati; Irwandi, Irwandi; Elfionita, Yolla Sarni
JURNAL KESEHATAN PERINTIS Vol 11 No 1 (2024): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v11i1.1067

Abstract

Bakteri endofit merupakan bakteri yang mampu menghasilkan senyawa-senyawa bioaktif yang sama dengan tanaman inangnya. Salah satunya tumbuhan yang potensial adalah pauh (Mangifera sumatrana Miq.). Tujuan dari penelitian ini yaitu untuk mengisolasi dan menguji aktivitas antimikroba dari bakteri endofit dari akar pauh terhadap Staphylococcus aureus, Escherichia coli, dan Candida albicans serta melakukan identifikasi secara molekuler menggunakan gen 16S rRNA terhadap bakteri endofit yang memiliki aktivitas antimikroba terkuat. Hasil penelitian ini didapatkan 4 isolat yaitu AL1, Al2, AD1, dan AD2 yang memiliki karakteristik morfologi berupa bentuk tidak beraturan dan bulat, permukaan timbul datar, tepian nya ada yang bergelombang, bergerigi, dan berserabut, serta warna koloni rata-rata putih susu. Hasil uji aktivitas antimikroba dengan metode Kirby-Bauer terhadap Staphylococcus aureus pada isolat AL1, AD1, dan AD2 maka didapatkan zona hambat sebagai berikut: 11,10 mm, 8,60 mm, dan 8,00 mm. Uji aktivitas terhadap Escherichia coli dan Candida albicans tidak memiliki aktivitas antimikroba. Identifikasi molekuler menunjukkan bahwa isolat AL memiliki aktivitas antimikroba terbesar dan memiliki kemiripan 99,91% dengan Lysinibacillus macrolides.
Desain Primer Dan Deteksi Gen Chs (Chalcone Synthase ) pada Tanaman Gambir (Uncaria gambir (Hunter) Roxb.) Tipe Udang Wardi, Epi Supri; Verawati, Verawati; Irma Juita, Atika; Nova, Bastian
FASKES : Jurnal Farmasi, Kesehatan, dan Sains Vol. 1 No. 2 (2023): Bulan November 2023 Faskes : Jurnal Farmasi, Kesehatan dan Sains
Publisher : Program Studi Farmasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32665/faskes.v1i3.2407

Abstract

Latar belakang: Gambir (Uncaria gambir (Hunter) Roxb.) merupakan tumbuhan asli Asia Tenggara terutama pulau Sumatera dan dibudidayakan terutama di daerah Sumatera Barat. Gen CHS (Chalcone synthase) merupakan salah satu enzim kunci yang terlibat dalam biosintesa katekin pada tanaman gambir. Tujuan: Penelitian ini bertujuan untuk mendapatkan primer yang dapat digunakan dalam deteksi gen CHS (chalcone synthase) pada tanaman gambir (Uncaria gambir (Hunter) Roxb) tipe Udang, serta untuk melihat apakah primer yang telah didesain dapat mendeteksi gen CHS (chalcone synthase) pada tanaman gambir (Uncaria gambir (Hunter) Roxb) tipe Udang. Metode: Tahapan penelitian ini meliputi pendesainan primer spesifik dan primer degeneratif, isolasi DNA tanaman gambir dengan metode CTAB, PCR ( Polymerase chain reaction) dan elektroforesis. Simpulan dan saran: Dari hasil penelitian didapatkan satu pasang kombinasi primer degeneratif yaitu : forward CHS-A1-F (5’-TNG TCT TCT GCA CAN CCT CCG GNG-3’) dan reverse CHS-C1-R (5’- CCA NTC CAA SCC YTC WCC DGT SGT-3’) yang memberikan hasil amplifikasi yang sesuai dengan estimasi produk sebesar 724 bp. Sehingga pasangan primer tersebut dapat dijadikan pilihan primer untuk mendeteksi gen CHS pada tanaman gambir (Uncaria gambir (Hunter) Roxb) tipe Udang.
Edukasi Sumber Alami Antioksidan dan Manfaatnya bagi Kesehatan di Desa Sungai Lantuang Verawati, Verawati; Fitrianda, Eka; Wahyuni, Rina
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 5, No 3 (2025): Abdira, Juli
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v5i3.696

Abstract

Antioxidants are natural or man-made substances that can prevent or delay some types of cell damage due to oxidation processes by free radicals in the body. Antioxidants are found in many foods, including fruits and vegetables. This community service aims to provide knowledge about natural sources of antioxidants and their benefits for health. This service activity was carried out for people who live in Sungai Latuang Village, Jambak Village, Batipuah Panjang Village, Padang City. The method used in this activity was counseling using the help of laptop and infocus devices. In addition to counseling, brochures and discussion sessions were also distributed as feedback to examine the participants' understanding of the material presented. Through this community service activity, there has been an increase in public understanding of the concept of antioxidants, natural sources of plants that are rich in antioxidant compounds, and their benefits for body health. This activity was attended by ± 30 people. Knowledge about natural antioxidants can add to people's insight into the importance of antioxidants in maintaining body health.
Co-Authors Almahdy Amilia Putri Arifah Nasir B.A. Martinus Bastian Nova Deddi Prima Putra Dedi Nofiandi Dira Arieska Dira Dira Dwi Fazillah Zalri Eka Fitrianda Elfionita, Yolla Sarni Febriyenti F. Fendri, Sandra Tri Juli Hanne Savera Intan Suci Mayasari Irma Juita, Atika Irwandi Irwandi Iyun Julia Ningsih Martinus BA Miftahur Rahmi Mimi Aria Okta Fera Petmawati Petmawati Putri Saimi Nuras Rina Wahyuni Riska Ramadhani Siska Ferilda Tisa Mandala Sari Wardi, Epi Supri Zaunit, Muthia Miranda