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EKSPLORASI POTENSI GULMA SIAM (CHROMOLAENA ODORATA) SEBAGAI BIOFUNGISIDA PENGENDALI PHYTOPHTHORA PALMIVORA YANG DIISOLASI DARI BUAH KAKAO Radix Suharjo; Titik Nur Aeny
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.629 KB) | DOI: 10.23960/j.hptt.211201-209

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The research was aimed to investigate: (1) distribution and population of Chromolaena odorata (siam weed) in different land-uses (paddy field, untreated land, houseyard, roadside and river/irigation side) in Bandar Lampung, South Lampung, Tanggamus, and East Lampung; (2) effect of C. odorata extract on in-vitro growth of Phytophthora palmivora, (3) effect of C. odorata extract on development of fruit rot symptom caused by P. palmivora on cocoa pod in the laboratory and in the field. Collecting of P. palmivora isolates and recording distribution and population of C. odorata were conducted in several locations known as endemic areas of cocoa pod rot disease. The effect of C. odorata extract on the growth of P. palmivora on agar media and cocoa pods was determine in the Laboratory of Plant Disease, Plant Protection Department, Faculty of Agriculture, University of Lampung. Field experiment was performed in a cocoa plantation in Kemiling, Bandar Lampung. The results showed that C. odorata weed can be found in all of the land-uses surveyed, but the highest population was found in the untreated land-use. The tips (young leaves and stems) extract of C. odorata showed a greater inhibition effect on the growth of P. palmivora compared to the root extract. The tips extract showed the best inhibition in-vitro growth of P. palmivora at 40% concentration level, inhibited symptom development on cocoa pods in the laboratory at 50% concentration level, and on cocoa pods in the field at 60% concentration level.
PENGARUH PERLAKUAN BIBIT TERHADAP PERKEMBANGAN PENYAKIT LAYU BAKTERI PADA JAHE (ZINGIBER OFFICINALE) Titik Nur Aeny, Feriansyah, dan Subli Mujim .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 2 No. 2 (2002): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.32 KB) | DOI: 10.23960/j.hptt.2260-64

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Effect of seedling treatments on development of bacterial wilt disease of ginger (Zingiber officinale). This experiment was aimed to study the effect of seedling treatment with bactericide on incubation period and disease intensity of bacterial wilt caused by Ralstonia solanacearum on ginger (Zingiber officinale). The experiment was conducted from May to November 2001, and the treatments were set as factorial experiment in completely randomised design. The first factor was the level of bactericide concentrations and the second factor was the length of soaking periods. Each treatment consisted of five plants and was replicated three times. Data were analysed with ANOVA and the means were compared with Duncan test. The results of analysis of variance showed that the interaction of bactericide concentrations with soaking periods was not significantly affected the incubation period and the intensity of bacterial wilt disease of ginger. However, those two factors significantly (P < 0.01) affected the incubation period and the intensity of ginger bacterial wilt disease. Separation of the means by Duncan test indicated that the higher the bactericide concentration, the longer the incubation period. On the other hand, the disease intensity was lowered by the higher concentration. The effect of bactericide concentration 4 g/l was not significantly different from that of 6 g/l. The analysis of variance was also showed that the length of soaking period of ginger seedling in bactericide did not affect the incubation period nor the disease intensity.
PATOGENISITAS BAKTERI LAYU PISANG (RALSTONIA SP.) PADA BEBERAPA TANAMAN LAIN Titik Nur Aeny1 .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 1 No. 2 (2001): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.389 KB) | DOI: 10.23960/j.hptt.2160-62

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ABSTRACTPathogenicity of banana wilt bacteria (Ralstonia sp.) on several other plants. This experiment was conducted to investigate the pathogenicity of bacterial wilt of banana pathogen (Ralstonia sp.) isolated from infected plants in Lampung on tomato, chili pepper, eggplant, and ginger. The experiment was conducted in Laboratory of Plant Protection Department, Faculty of Agricultural University of Lampung, from May 1999 to February 2000. Treatments in this experiment were arranged in a completely randomized design with four replications. The treatments were tomato, eggplant, chili pepper, and ginger. Banana was used as the control. The results of this experiment showed that up to 30 days after inoculation, the bacteria isolated from banana found in Lampung was not able to cause symptom on tomato, chili pepper, eggplant, nor ginger. However, this bacteria caused wilting on banana eight days after inoculation; the incubation period of this bacteria on banana was eight days.
Pineapple Fruit Collapse: Newly Emerging Disease of Pineapple Fruit in Lampung, Indonesia Joko Prasetyo; Titik Nur Aeny
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.151 KB) | DOI: 10.23960/j.hptt.11496-99

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ABSTRACTPineapple fruit collapse: newly emerging disease of pineapple fruit in Lampung, Indonesia Recently, a new disease on pineapple fruit has occurred in Lampung. Symptoms of the disease are complex. Fruits rotted and exuded copious liquid from the inter- fruitlet tissues accompanied by gas bubbles. Open spaces were formed inside the rotten fruit. Dissection of diseased fruit showed many cavities within its sceletal fibres and bad odour was exerted from the rotten tissues. A bacterial entity was isolated from the diseased materials. In a pathogenicity test, the isolated bacteria caused the same symptom as mentioned. In the growing-on test the crown of the heavily infected fruit showed heart rot symptom. Those indicated that the disease was pineapple fruit collapse. Both symptoms were known related to the same causal agent, Erwinia chrysanthemi (pineapple strain Dickeya sp.). In our opinion, this is the first report of pineapple fruit collapse in Indonesia.
Bioakumulasi dan Aktivitas Resistensi Logam Timbal (Pb) terhadap Streptomyces sp. strain I18 Mutia Dinda Lestari; Mesy Miranda AR; Ulin Ni&#039;mah Setiawati; Nismah Nukmal; Endah Setyaningrum; Achmad Arifiyanto; Titik Nur Aeny
Jurnal Sumberdaya Alam dan Lingkungan Vol 9, No 1 (2022)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsal.2022.009.01.1

Abstract

ABSTRAKKegiatan antropogenik secara intensif mengakibatkan pencemaran lingkungan yang disebabkan oleh logam berat. Timbal (Pb) merupakan salah satu logam berat toksik. Tingkat toksisitas dapat diturunkan melalui proses bioakumulasi oleh mikroorganisme. Penelitian ini dilakukan untuk mengetahui kemampuan bakteri Streptomyves sp. strain I18 dalam mengakumulasi logam Pb. Bakteri diidentifikasi morfologi secara makroskopik dan mikroskopik. Daya resistensi bakteri terhadap logam Pb ditentukan dengan menumbuhkan bakteri pada media Muller-Hilton Agar yang disuplementasi Pb pada konsentrasi 5, 50, dan 150 ppm menggunakan metode dilusi pada cawan petri petri. Bioakumulasi bakteri terhadap logam Pb diukur menggunakan Atomic Absorption Spectroscopy (AAS) pada konsentrasi 5, 50, dan 150 ppm. Bakteri Streptomyves sp. strain I18 mampu tumbuh pada media padat agar mengandung logam Pb pada bagai konsentrasi dengan diameter koloni terbesar, yakni 50.33 mm pada konsentrasi Pb 5 ppm. Pertumbuhan koloni terkecil sebesar 33.00 mm terjadi pada konsentrasi 150 ppm. Bakteri ini terbukti mampu mengakumulasi logam Pb dengan akumulasi terbesar pada konsentrasi 5 ppm, sebesar 49%. Peningkatan konsentrasi logam menurunkan kemampuan akumulasi logam Pb. Pada konsentrasi 150 ppm dan 50 ppm, bakteri hanya mampu mengakumulasi Pb sebesar 5% dan 9%. Kemampuan optimal bakteri Streptomyves sp. strain I18 sebagai bioakumulator diperkirakan pada konsentrasi kurang dari 50 ppm.Kata kunci: bioakumulasi, Pb, resistensi, Streptomyces ABSTRACTAntropogenic activity intensively can cause environmental pollution by heavy metals. Lead is one of toxic heavy metal. Level of toxicity can be decreased with bioaccumulation by microorganisms. This research conducted to know the ability of bacteria Streptomyces sp. strain I18 to accumulate lead. The morphological of bacteria was identified by microscopic and macroscopic. Bacterial resistance of lead has been known by growing bacterial in Muller-Hilton Agar supplemented lead in concentration 5, 50, and 150 ppm uses dilution plate method. Biaccumulation of bacteria against lead measured by Atomic Absorption Spectroscopy (AAS). Streptomyces sp. strain I18 can grow in solid agar media contains lead in various concentration. The highest diameter of colony 50.33 mm measured in media combined lead concentration 5 ppm. The lowest growth of colony is 33.00 mm in lead concentration of 1000 ppm. These bacteria are proven that can accumulate lead with the highest accumulation reached 49% in concentration of 5 ppm. The concentration of 150 and 50 ppm can accumulate lead reached 5% and 9%. Optimal ability of bacteria Streptomyces sp. strain I18 as bioaccumulator supposed in concentration of less than 50 ppm.Keywords: bioaccumulation, lead, resistance, Streptomyces
PENGARUH BEBERAPA JENIS FUNGISIDA SEBAGAI PERLAKUAN BENIH JAGUNG TERHADAP KELIMPAHAN DAN KERAGAMAN NEMATODA Lisa Septiani; I Gede Swibawa; Puji Lestari; Titik nur Aeny
Jurnal Agrotek Tropika Vol 6, No 1 (2018)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.617 KB) | DOI: 10.23960/jat.v6i1.2530

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Jagung merupakan salah satu tanaman pangan penting di Indonesia dan mempunyai peran strategis dalam perekonomian nasional. Pengembangan tanaman jagung masih terkendala oleh rendahnya ketahanan tanaman terhadap gangguan hama dan penyakit. Penyakit yang banyak mengganggu tanaman jagung yaitu penyakit bulai yang disebabkan oleh jamur Perenosclerospora spp. Salah satu cara pengendalian penyakit bulai yaitu menggunakan fungisida sebagai perlakuan benih. Penggunaan fungisida sebagai perlakuan benih dapat mengganggu aktivitas biota tanah seperti nematoda. Tujuan penelitianiniadalah untuk mempelajari pengaruh beberapa jenis fungisida sebagai perlakuan benih terhadap kelimpahan dan keragaman nematoda pada pertanaman jagung. Penelitian ini dilaksanakan di Natar Kabupaten Lampung Selatan dan proses laboratorium dilakukan di Laboratorium Ilmu Hama Tumbuhan, Fakultas Pertanian, Universitas Lampung, mulai bulan Februari sampai dengan Juni 2016. Perlakuan dalam percobaan disusun dalam Rancangan Acak Kelompok (RAK), dengan pengelompokan dilakukan berdasarkan arah mata angin. Fungisida yang digunakan sebagai perlakuan benih yaitu Hab, Dimetomorf 50%, Fungisida 3, Fenimidon, Fungisida 2, Fungisida 4, Metalaksil 35%, Fungisida 1, dan Imax. Data kelimpahan dan keragaman nematoda dianalisis kovarian pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa perlakuan benih tanaman jagung dengan fungisida menurunkan kelimpahan seluruh nematoda, tetapi tidak mempengaruhi kelimpahan kelompok makannematoda. Perlakuan benih tanaman jagung dengan fungisida tidak mempengaruhi keragaman nematoda.
PENGARUH FRAKSI EKSTRAK DAUN BABADOTAN (Ageratum conyzoides) TERHADAP PERTUMBUHAN DAN SPORULASI Colletotrichum capsici SECARA IN VITRO Shintya Wulandari; Titik Nur Aeny; Efri Efri
Jurnal Agrotek Tropika Vol 3, No 2 (2015)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.025 KB) | DOI: 10.23960/jat.v3i2.2002

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Penyakit antraknosa yang disebabkan oleh Colletotrichum capsici merupakan salah satu penyakit penting pada tanaman cabai. Salah satu cara pengendalian penyakit antraknosa yang ramah lingkungan adalah penggunaan fungisida nabati atau fungisida yang berasal dari ekstrak daun atau bagian-bagian tanaman lain. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak daun babadotan yang difraksinasi dengan pelarut air, metanol, etil asetat dan n-heksana dalam menekan  pertumbuhan dan sporulasi C. capsici secara in vitro. Penelitian ini dilakukan di Laboratorium Penyakit Tanaman, Fakultas Pertanian, Universitas Lampung pada bulan September sampai dengan Desember 2014. Rancangan perlakuan yang digunakan adalah rancangan acak lengkap, dengan 6 perlakuan dan 5 ulangan. Keenam perlakuan tersebut yaitu perlakuan tanpa menggunakan ekstrak, ekstrak tanaman uji dengan pelarut air, metanol, etil asetat dan n-heksana serta fungisida sintetik berbahan aktif propineb 70% . Hasil penelitian menunjukkan bahwa fraksi ekstrak daun babadotan dengan pelarut metanol menunjukkan hasil yang paling baik dalam menghambat pertumbuhan dan sporulasi C. capsici secara in vitro. Namun, keefektivan fraksi ekstrak tersebut lebih rendah dibandingkan dengan fungisida sintetik berbahan aktif propineb 70%.
PENGARUH FRAKSI EKSTRAK DAUN TAGETES (Tagetes erecta) TERHADAP PERTUMBUHAN DAN SPORULASI Colletotrichum capsici SECARA IN VITRO M Wahyu Satryawibowo; Efri Efri; Titik Nur Aeny
Jurnal Agrotek Tropika Vol 3, No 2 (2015)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.324 KB) | DOI: 10.23960/jat.v3i2.1998

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Pada umumnya pengendalian penyakit antraknosa dilakukan dengan menggunakan fungisida kimia sintetik yang selalu menimbulkan dampak negatif. Salah satu alternatif pengendalian yang ramah lingkungan untuk mengendalikan penyakit tanaman adalah menggunakan fungisida nabati. Penelitian ini bertujuan untuk mengetahui efektivitas fraksi ekstrak dauntagetes sebagai fungisida nabati dalam menekan pertumbuhan dan sporulasi patogen antraknosa pada cabai yaitu C. capsici secara in vitro. Penelitian ini dilaksanakan di Laboratorium Penyakit Tanaman, Fakultas Pertanian, Universitas Lampung pada bulan Juni sampai dengan September 2014. Rancangan percobaan yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL). Percobaan terdiridari enam perlakuan dan lima ulangan. Perlakuan adalah tanpa menggunakan ekstrak (P1), perlakuan dengan fungisida propineb (P2), fraksi ekstrak daun tagetes dengan pelarut air (P3), fraksi ekstrak daun tagetes dengan pelarut metanol (P4), fraksi ekstrak daun tagetes dengan pelarut etil asetat (P5), fraksi ekstrak daun tagetes dengan pelarut n-hexana (P6). Hasil penelitian menunjukkan bahwa fraksi ekstrak daun tagetes dengan pelarut metanol berpotensi sebagai fungisida nabati lebih baik dibandingkan dengan fraksi ekstrak daun tagetes dengan pelarut air dan pelarut etil asetat walaupun pengaruhnya tidak sebaik fungisida propineb terhadap penghambatan pertumbuhan dan sporulasi C. capsici secara in vitro.
ISOLASI DAN KARAKTERISASI PENYEBAB PENYAKIT BUSUK BUAH PADA TANAMAN NANAS (Ananas comosus [L.] Merr.) Tiya Oviana; Titik Nur Aeny; Joko Prasetyo
Jurnal Agrotek Tropika Vol 3, No 2 (2015)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.375 KB) | DOI: 10.23960/jat.v3i2.2001

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Nanas (Ananas comosus L. Merr.) merupakan salah satu komoditas hortikultura yang mendominasi perdagangan buah tropika dunia. Indonesia merupakan negara yang menempati posisi ketiga sebagai negara penghasil nanas olahan dan segar setelah Thailand dan Filipina. Salah satu kendala dalam peningkatan produksi nanas adalah adanya penyakit busuk buah (fruit collapse) yang disebabkan oleh bakteri patogen. Penelitian ini bertujuan untuk mengisolasi penyebab penyakit busuk buah nanas dari perkebunan PT Nusantara Tropical Farm (NTF) dan mengetahui identitas patogen tersebut. Pelaksanaan penelitian ini terdiri dari dua tahap, yaitu tahap pertama berupa isolasi dari jaringan buah dan crown nanas yang sakit untuk mendapat isolat-isolat murni. Tahap kedua adalah karakterisasi yang dilakukan melalui uji morfologi,biokimia, dan patogenisitas untuk mengetahui karakteristik isolat patogen yang diperoleh. Dari hasil isolasi diketahui bahwa penyebab penyakit busuk buah nanas yang ada di PT NTF adalah bakteri. Isolat bakteri tersebut merupakan bakteri Gram negatif, bersifat soft rot, anaerob fakultatif dan virulen. Gejala penyakit antara lain berupa busuk buah, pada daun terbentuk garis-garis berwarna coklat dan melepuh. Berdasarkan gejala yang khas pada daun tanaman sakit serta hasil karakterisasi isolat bakteri, diduga bahwa penyebab penyakit busuk buah pada tanaman nanas di perkebunan PT NTF adalah bakteri Erwinia chrysanthemi.
IMPLEMENTASI SISTEM PAKAR PADA PENYAKIT BUAH PEPAYA CALIFORNIA BERBASIS ANDROID MENGGUNAKAN METODE FORWARD CHAINING Irwan Adi Pribadi; Titik Nur Aeny; Akmal Junaidi; Tio Arisandi
Jurnal Pepadun Vol. 3 No. 1 (2022): April
Publisher : Department of Computer Science, Faculty of Mathematics and Natural Sciences, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/pepadun.v3i1.101

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California papaya is one of the varieties of papaya developed by the Bogor Agricultural Institute known as IPB-9. California papaya is widely cultivated by farmers in Indonesia because it is quite profitable, but this plant is not immune from disease. For this reason, farmers must understand the aspects of california papaya disease so that their hard work from california papaya farming brings satisfactory results. With the advancement of science and information technology today, farmers can find out information about california papaya disease and how to control it easily. This research produces an expert system application that can find out information about california papaya disease by using the forward chaining method. Farmers can use various features in this california papaya application. The first feature is disease diagnosis. In this feature, farmers will choose the symptoms that have been provided by the application, totaling 28 symptoms which will then be drawn conclusions in the form of results and ways to overcome the disease concluded. The second feature, the application provides a list of 9 diseases in california papaya so that farmers can find out in full about the disease and how to overcome it.