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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Hortikultura Indonesia (JHI) Kultivasi Agrosains: Jurnal Penelitian Agronomi Jurnal Pertanian Agros Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Sains Agro Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Andalasian International Journal of Agricultural and Natural Sciences Jurnal Riset Perkebunan (JRP) Journal of Applied Agricultural Science and Technology JATIMAS : Jurnal Pertanian dan Pengabdian Masyarakat Jagur: Jurnal Agroteknologi Indonesian Journal of Forestry Research
Ryan Budi Setiawan
Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Medicine & Pharmacology Public Health Social Sciences Other

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IBA-induced rooting in Nepenthes reinwardtiana Miq. cuttings for germplasm conservation Ryan Budi Setiawan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 1 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i1.60254

Abstract

Nepenthes reinwardtiana Miq. is an endemic species distributed in Sumatra and Kalimantan and classified as least concern due to the lack of recent research data on its abundance and distribution. Excessive exploitation, deforestation, the long time required to reach the generative phase, and the dioecious flowering type have caused a population decline. Plant propagation through cuttings can support conservation activities. The success of cuttings is determined by the concentration of plant growth regulators (PGR), making studies on PGR concentration important. This research aimed to determine the best indole-3-butyric acid (IBA) concentration for inducing roots and shoots in N. reinwardtiana. The study was conducted from February to October 2023. The research was arranged based on a completely randomized design with IBA concentration treatments consisting of 5 levels: 0, 5, 10, 15, and 20 mg L-1. The results showed that there was no significant effect of IBA on all variables. However, 5 mg L-1 IBA gave the best responses on shoot height increase (10.9 cm), leaf number increase (5.0 leaves), and root length (8.7 cm). These findings suggest that the growth of N. reinwardtiana cuttings may rely heavily on endogenous auxin levels, reducing the observable effects of exogenous IBA application. The non-significant results highlight the potential of this species to thrive without external auxin under certain conditions, which could simplify propagation protocols. Keywords: biodiversity; conservation; endemic species; extinct; plant growth regulator
The Effect of Benzyl Amino Purine (BAP) on Indirect Organogenesis of the Titan Arum (Amorphophallus titanum (Becc.)) Afifah, Putri Salsa; Yusniwati, Yusniwati; Zainal, Aprizal; Setiawan, Ryan Budi
Agrosains : Jurnal Penelitian Agronomi Vol 26, No 2 (2024): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v26i2.95804

Abstract

The titan arum (Amorphophallus titanum (Becc.)), an endangered and endemic flora from the island of Sumatra, faces a significant risk of extinction. Conservation efforts for the titan arum were undertaken, including in vitro conservation through tissue culture. The study aimed to determine the optimal concentration of the growth regulator Benzyl Amino Purine (BAP) for inducing shoot formation in titan arum and to observe cellular changes during the shoot stage through histological test. This research was conducted from November 2023 to March 2024 at the Tissue Culture Laboratory, Andalas University. The experiment was arranged in a Completely Randomized Design (CRD) with five treatment levels: 2, 3, 4, 5, and 6 ppm.  Data analysis was performed using an F-test at a 5% significance level. If the F-test showed significant differences, further testing was carried out using Duncan's Multiple Range Test (DMRT) at a 5% significance level. Meanwhile, data on explant rooting percentage and root count were presented as means and standard deviations. Results indicated that various concentrations of BAP could induce shoots formation of Amorphophallus titanum Becc., with the percentage of explants forming shoots reaching 80–95%.  The concentration of 5.0 ppm BAP was the most effective, yielding the highest average number of shoots at 7.80. Histological test revealed cell enlargement at the shoot tips. This research is pivotal for the conservation of titan arum and promotes further studies in in vitro culture techniques.
Co-Authors . Reflinaldon Adhira Prameswari Ranayudha Afifah, Putri Salsa Afrima Sari Agil Syah Putra Aprizal Zainal Ardi Ardi Arief Laksana Bosma, Pandu Aries Kusumawati Aries Novita Putri ARIF KURNIAWAN Awang Al Hamdi Baiturrahman, Atzim Benni Satria Bosma, Pandu Arif Laksana Cecep Taupik Hidayat Dea Hayu Nastiti Dian Sukma, Dian Diny Dinarti Doni Hariandi Ekasari Putri, Nurwanita ekawati, fitri G Gustian Gustian Gustian Handayani, Mellyyana Haryanti, Sindi Hayati, PK Dewi I Imelinda Indra Dwipa Irawati Chaniago Jumsalia Juniarti Juniarti Kelvin Rinaldi Siregar Khairur Rizki Meisilva Erona Sitepu Muhammad Rafli Ibrahim Nadila Aulia Ardi Nalwida Rozen Nanda Nur’aini Nanda, Alifma Rahimmi Nandita Samaralya Tori Nurul Khumaida Purnama Sari, Indah Rahimmi Nanda, Alifma Rahma Wati, Dela Rahmi, Alfazlul Rizah Rizwana Wahyuni Rizki, Khairur Sari, Winda Purnama Sembiring, Rinawati Shinta Mulyana Silvia Permata Sari Siti Rahmah Syahputra, Agil Teguh Saputra Ummi Hajra Dias Yaherwandi Yusniwati Yusniwati Yusniwati Zulfadly Syarif