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All Journal Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Padjadjaran Journal of Dental Researchers and Students SONDE (Sound of Dentistry) Jurnal Material Kedokteran Gigi Odonto dental journal Enrichment: Journal of Multidisciplinary Research and Development
Diani Prisinda
Departemen Ilmu Konservasi Gigi, Fakultas Kedokteran Gigi, Universitas Padjadjaran, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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The MIC and MBC of calcium hydroxide medicament against bacteria that cause chronic periapical abscess in the vulnerable initial 7-days of endodontic treatment Putri, Chany Mony Dwiayu; Prisinda, Diani; Malinda, Yuti
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.28638

Abstract

Introduction: The primary aetiology of chronic periapical abscesses, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis, can be eliminated using intracanal medicaments such as calcium hydroxide. The purpose of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of calcium hydroxide paste in the vulnerable initial 7-day of treatment against E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277. Methods: An in-vitro laboratory experiment using a spectrophotometer as a microdilution method was conducted to determine bacteria's MIC and MBC values on calcium hydroxide paste. The sample used in this study was four bacteria using intracanal medicament of calcium hydroxide (Ca(OH)2), incubated for seven days at 37°C, and then bacterial growth was observed. The cell inhibition percentage was calculated using optical density measurements to determine the MIC value. The low MIC and MBC were defined as sensitive bacteria to calcium hydroxide. Results: Calcium hydroxide paste against E. faecalis (ATCC 29212) with MIC values at a concentration of 750 μg/ml and MBC values at a concentration of 96,000 μg ml; S. mutans (ATCC 25175) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 48,000 ug/ml; S. sanguinis (ATCC 10556) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 6,000 ug/ml; P. gingivalis (ATCC 33277) with MIC value at a concentration of 6,000 ug/ml and MBC value at a concentration of 48,000 ug ml. Conclusions: Calcium hydroxide can inhibit bacterial growth activity. E. faecalis (ATCC 29212) and S. sanguinis (ATCC 10556) are more sensitive to calcium hydroxide paste than other bacteria, with the lowest MIC and MBC on seven days of incubation since the maximum calcium and hydroxyl ions are released.
Effectiveness of ChKM solution compared to triple-antibiotic paste as an intracanal medicament for bacteria that cause a chronic periapical abscess Malinda, Yuti; Azhara, Dea Hanin; Harnung, Kartikaning; Prisinda, Diani
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.28642

Abstract

Introduction: Chronic periapical abscesses are caused by polymicrobial bacteria, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis. These bacteria can be eliminated with chlorophenol camphor menthol (ChKM) solution and triple-antibiotic paste (TAP) as an intracanal medicament. This study compared the effectiveness of ChKM solution to triple-antibiotic paste as an intracanal medicament for bacteria that cause a chronic periapical abscess. Methods: An experimental laboratory was conducted in-vitro with the microdilution method and optical density measurement using a spectrophotometer. The cell inhibition percentage was calculated to determine the MIC value. The MBC values were confirmed by cultivating the Mueller Hinton Agar samples, incubating them at 37°C for 24 hours, and observing bacterial growth. Bacteria did not grow in the medium at MBC value. The lower MIC and MBC were sensitive and could be an effective medicament choice. Results: The MIC ChKM solution inhibits E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277 were 4000, 4000, 2000, and 2000 µg/mL consecutively. While MIC of TAP were 6, 0.375, 0.75, and 1.5 µg/mL, respectively. Moreover, MBC of the ChKM solution were 32000, 32000, 8000, 8000 µg/mL and MBC of the TAP were 768, 24, 24, 96 µg/mL. Conclusions: ChKM solution and TAP effectively inhibited and killed E. faecalis, S. mutans, S. sanguinis, and P. gingivalis as an intracanal medicament, as seen from the MIC and MBC values. However, TAP is more effective than the ChKM solution because MIC and MBC values of TAP are much lower than the ChKM solution. This finding indicated that TAP is more effective at the lowest concentration than ChKM solution. It should be highlighted that this was an in-vitro study involving specific microbes; thus, further clinical research is needed.
Tindakan endodontik sustainable dengan perawatan saluran akar satu kali kunjungan pada gigi molar kedua kanan bawah: Laporan kasus Kesuma, Alex; Prisinda, Diani
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 4 (2024): Januari 2024 (Suplemen 4)
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i4.49842

Abstract

ABSTRAKPendahuluan: Perawatan kesehatan gigi dan mulut yang optimal terkait dengan sustainability (pelestarian lingkungan) dalam kedokteran gigi bertujuan untuk mencapai praktik kedokteran gigi yang berkualitas dengan dampak negatif yang minimal terhadap lingkungan. Dalam dunia endodontik, salah satu upaya sederhana yang dapat dilakukan oleh dokter gigi saat melakukan perawatan saluran akar adalah dengan menyelesaikan perawatan dalam satu kali kunjungan jika memenuhi indikasi secara klinis dan memungkinkan secara teknis, sehingga dapat mengurangi penggunaan peralatan maupun bahan kedokteran gigi serta jumlah kunjungan pasien. Laporan kasus ini bertujuan untuk menyajikan tindakan perawatan saluran akar satu kali kunjungan pada gigi molar kedua kanan mandibula sebagai salah satu wujud tindakan sustainable dalam bidang endodontik. Laporan kasus: Seorang pasien perempuan berusia 22 tahun datang ke poliklinik konservasi gigi RSGM UNPAD Bandung untuk memperoleh perawatan terhadap keluhan gigi berlubang pada geraham kedua kanan bawahnya dengan riwayat nyeri spontan dan berdenyut yang berlangsung selama beberapa waktu. Pasien mengkonsumsi analgesik untuk meredakan gejalanya sementara waktu. Hasil pemeriksaan objektif menunjukkan karies profunda pada disto-oklusal gigi 47 dengan uji sensibilitas positif, disertai uji perkusi, gigitan, palpasi, dan kegoyangan dalam batas normal. Diagnosis yang ditegakkan adalah pulpitis ireversibel simptomatik dan jaringan apikal normal pada gigi 47. Rencana perawatan adalah berupa perawatan saluran akar satu kali kunjungan dilanjutkan dengan restorasi intermediate komposit direk, kemudian pasien direncanakan untuk dirujuk ke bagian prosthodontik untuk dibuatkan protesa cekat. Simpulan: Salah satu upaya tindakan sustainable dalam bidang endodontik dapat dilakukan dengan melakukan perawatan saluran akar satu kali kunjungan dan pada laporan kasus ini memperlihatkan tingkat keberhasilan perawatan yang tinggi.
Co-Authors Ame Suciati Setiawan Ame Suciati Setiawan Ame Suciati Setiawan Ame Suciati, Ame Andriani Harsanti, Andriani Anna Muryani Azhara, Dea Hanin Cindy Lestari Marshaliana Dani Rizali Firman Ellizabeth Yunita Fajar Fatriadi Fajar Fitriadi Fidya Meditia Putri haifa nawari, bilqis Harnung, Kartikaning Hendra Dian Adhita Dharsono Indah Suasani Wahyuni Ing. Mochammad Agoes Moelyadi Kesuma, Alex Lydiawati Wibisono Marcelina Wijaya Maria Faizarani Nanan Nuraeny Prima Andisetyanto Prima Dianiawati Nur Anisa Putri, Chany Mony Dwiayu Rahmi Wastri Sheilla Fauzia Maretta Sri Tjahajawati, Sri Wahyu Hidayat Wilam, Christiana Yuliawati Zenab Yurika Ambar Lita Yuti Malinda Z. Zuleika