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AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Setiawan, Ame Suciati; Fatriadi, Fajar; Prisinda, Diani
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.2.111-116

Abstract

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.
Perawatan Endodontik Pada Kasus Kombinasi Resorpsi Internal Dan Resorpsi Servikal Gigi Insisivus Sentral Rahang Atas Marcelina Wijaya; Diani Prisinda
SONDE (Sound of Dentistry) Vol. 6 No. 1 (2021): SONDE (Sound of Dentistry)
Publisher : Maranatha Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28932/sod.v6i1.3643

Abstract

Pendahuluan: Resorpsi merupakan kondisi terkait proses fisiologis atau patologis yang mengakibatkan hilangnya dentin, sementum, atau tulang karena interaksi terstruktur antara sel inflamasi dan sel resorpsi, dapat terjadi baik internal maupun eksternal, secara bersamaan pada gigi yang sama, muncul sebagai defek yang terpisah atau bergabung. Proses resorpsi dimulai oleh berbagai rangsangan seperti trauma, peradangan kronis pada pulpa terus menerus yang disebabkan oleh faktor bakteri dan perawatan ortodontik. Laporan Kasus: Pasien laki-laki usia 27 tahun datang dengan keluhan gigi atas depan kanan berubah warna sedikit lebih gelap dibandingkan gigi sebelahnya. Gigi tersebut pernah sakit tiba-tiba dan pernah muncul jerawat di sekitar gusinya. Pasien pernah mengalami kecelakaan motor dan gigi depannya terbentur 10 tahun yang lalu, pernah dirawat ortodonti cekat selama 4 tahun. Pemeriksaan radiografis gigi 11 menunjukkan mahkota normal, jumlah akar 1, terdapat radiolusen pada 1/3 hingga 2/3 tengah dinding saluran akar mesial, lamina dura menghilang di periapikal dan pada 2/3 tengah akar dinding mesial, dan terdapat radiolusen berbatas jelas dengan diameter 2mm. Gigi 11 dilakukan pembukaan akses kavitas, finishing menggunakan nikel titanium rotary ProTaper Gold (Dentsply), irigasi dengan EDTA 17% dan NaOCl 5,25% serta aktivasi menggunakan ultrasonik (Ultra X, Eighteeth), pemberian medikamen antar kunjungan kalsium hidroksida, selanjutnya pengisian saluran akar dengan teknik warm vertical condensation. Restorasi pasca endodontik yaitu resin komposit direk dengan resin fiber construct (Kerr). Simpulan: Perawatan endodontik kombinasi resorpsi internal dan resorpsi servikal gigi insisivus sentral rahang atas pada kasus ini memperlihatkan keberhasilan yang baik.
Effectiveness of Calcium Hydroxide Against Enterococcus Faecalis Biofilm in Dental Root Canals bilqis haifa nawari; Yuti Malinda; Diani Prisinda
Enrichment: Journal of Multidisciplinary Research and Development Vol. 2 No. 11 (2025): Enrichment: Journal of Multidisciplinary Research and Development
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/enrichment.v2i11.300

Abstract

Enterococcus faecalis (E. faecalis) is the most widely detected species associated with persistent endodontic infections because it has the ability to form biofilms. This study aimed to identify the effectiveness of calcium hydroxide paste CalcigelĀ® (Prevest DenPro, India) on E. faecalis (ATCC 29212) biofilms in root canals with exposure times of 14, 21, and 28 days. The type of research used was true experimental in vitro by randomly selecting samples of tooth roots on the 14th day of biofilm development and dividing them into six groups: use of Ca(OH)2 as intra-canal medicament for 14, 21, and 28 days, as well as positive control without treatment for 14, 21, and 28 days. Dentin chip suspensions were used for colony form ng units counting to estimates remaining E. faecalis counts. The effectiveness calculation obtained by comparing the test group with the control group at the same treatment duration, with the results of using Ca(OH)2 for 14 days showing a decrease in the number of E. faecalis 96.46%, the results of using Ca(OH)2 for 21 days was 95.20%, while the results of using Ca(OH)2 for 28 days was 94.59%. The results of testing the hypothesis with the ANOVA test showed that there was no significant difference between the effectiveness of calcium hydroxide with exposure to 14 days, 21 days, and 28 days with a significance value of <0.452.