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All Journal Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Padjadjaran Journal of Dental Researchers and Students SONDE (Sound of Dentistry) Jurnal Material Kedokteran Gigi Odonto dental journal Enrichment: Journal of Multidisciplinary Research and Development
Diani Prisinda
Departemen Ilmu Konservasi Gigi, Fakultas Kedokteran Gigi, Universitas Padjadjaran, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Setiawan, Ame Suciati; Fatriadi, Fajar; Prisinda, Diani
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.559 KB) | DOI: 10.30659/odj.7.2.111-116

Abstract

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.
THE ANTIBIOTICS SENSITIVITY TEST ON STAPHYLOCOCCUS AND STREPTOCOCCUS FROM CHRONIC APICAL ABSCESS Malinda, Yuti; Prisinda, Diani
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.1.130-137

Abstract

Background: Prophylactic antibiotics are required as part of root canal therapy for patient with chronic apical abscess. Streptococcus and Staphylococcus are the most common bacteria found in root canals with chronic apical abscess. Resistance to amoxicillin, clindamycin and vancomycin has been increasing year after year, necessitating the use of a sensitivity test. The goal of this research was to determine the sensitivity of Streptococcus and Staphylococcus from root canals with chronic apical abscess to amoxicillin, clindamycin, and vancomycin to make scientific antibiotic selections.Method: Six isolates of Staphylococcus and one Streptococcus from root canals with chronic apical abscess were identified by Remel RapID and tested to 10 g amoxicillin, 2 g clindamycin, and 30 g vancomycin using Kirby-Bauer disk-diffusion sensitivity test. The diameter inhibition zone was measured and interpreted according to the CLSI (Clinical and Laboratory Standards Institute) standard.Result: Three of six Staphylococcus are resistant, one of six are intermediate, and two are sensitive to amoxicillin. While the Streptococcus is sensitive to amoxicillin. In contrast, all Staphylococcus and the Streptococcus are resistant to clindamycin. Whilst four of six Staphylococcus are resistant, two are intermediate, and the Streptococcus is resistant to vancomycin.Conclusion: Staphylococcus sensitivity to amoxicillin and vancomycin are diverse even though resistant to clindamycin. In comparison, the Streptococcus sensitivity to clindamycin and vancomycin is resistant but sensitive to amoxicillin.
AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Setiawan, Ame Suciati; Fatriadi, Fajar; Prisinda, Diani
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.2.111-116

Abstract

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.
Co-Authors Ame Suciati Setiawan Ame Suciati Setiawan Ame Suciati Setiawan Ame Suciati, Ame Andriani Harsanti, Andriani Anna Muryani Azhara, Dea Hanin Cindy Lestari Marshaliana Dani Rizali Firman Ellizabeth Yunita Fajar Fatriadi Fajar Fitriadi Fidya Meditia Putri haifa nawari, bilqis Harnung, Kartikaning Hendra Dian Adhita Dharsono Indah Suasani Wahyuni Ing. Mochammad Agoes Moelyadi Kesuma, Alex Lydiawati Wibisono Marcelina Wijaya Maria Faizarani Nanan Nuraeny Prima Andisetyanto Prima Dianiawati Nur Anisa Putri, Chany Mony Dwiayu Rahmi Wastri Sheilla Fauzia Maretta Sri Tjahajawati, Sri Wahyu Hidayat Wilam, Christiana Yuliawati Zenab Yurika Ambar Lita Yuti Malinda Z. Zuleika