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Successful test dry heat oven sterilizer on dental instruments sterilization in X Hospital Amir, Arrahmi; Dewi, Warta; Wardani, Riana
Padjadjaran Journal of Dentistry Vol 25, No 3 (2013): November 2013
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol25no3.26805

Abstract

Introduction: Instruments used for dental examinations and treatment often come into contact with fluids and soft tissues found in the oral cavity. The contact while the dental treatment procedures can cause contaminated and potentially mediates the displacement of microorganisms from the mouth of the patient to the operator or to other patients. Sterilization is the most effective process for the decontamination of reusable equipment. The objective of this study was testing the success of dry heat oven sterilizer to dental instruments sterilization in X Hospital. Methods: This study used descriptive method. Data was obtained by bacteriological examination, with a random sampling technique and obtained a sample of five instruments. Results: The results show there is a growing colony of bacteria in each sample after examination materials were incubated at 37 ºC for 18-24 hours. Conclusion: The conclusions of this study are dry heat oven sterilizer not belong to the success criteria for dental instrument sterilization process in X Hospital.
Antibacterial efficacy of Rosella (Hibiscus sabdariffa Linn) flower extract against Streptococcus sanguis Nafisa, Durotun; Dewi, Warta; Rachmawati, Emma
Padjadjaran Journal of Dentistry Vol 27, No 1 (2015): March 2015
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol27no1.26691

Abstract

Introduction: Rosella is well known as health drink that contains anti bacterial compounds. The purpose of this research is to examine the anti bacterial potential of rosella calyx ethanol extract towards Streptococcus sanguis. Methods: Streptococcus sanguis was obtained from students’ saliva in the clinic of the Faculty of Dentistry, Universitas Padjadjaran. It was cultured in blood agar and incubated for 18 to 24 hours at 37°C in a facultative anaerobic environment. Streptococcus sanguis was isolated based on the characteristics of the colonies formed, Gram staining, inulin and rafinose fermentation tests. The anti bacterial test was performed using agar diffusion method (Kirby Bauer Method) by making agar holes in the agar and filling them with rosella extract with concentrations at 0.20 g/ml, 0.10 g/ml, 0.05 g/ml and 0.025 g/ml. Results: The results of this research show that there was anti bacterial activity from the ethanol extract of rosella calyx with the inhibition zones of 19.85 mm, 12.05 mm, 8.45 mm and 3.65 mm under rosellas’s extract’s concentration of at 0.20 g/ml, 0.10 g/ml, 0.05 g/ml and 0.025 g/ml respectively. Conclusion: The conclusion of this research is rosella calyx ethanol extract has the greatest anti bacterial activity at concentration 0.20 g/ml.
Antibacterial activity testing of ethanolic extract of aloe vera leaf and gel against methicillin-resistant Staphylococcus aureus Pratiwi, Tias Eka; Dewi, Warta; Prijono, Eddy
Padjadjaran Journal of Dentistry Vol 26, No 2 (2014): July 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol26no2.26702

Abstract

Introduction: The interest on Aloe vera based herbal products is fast growing during recent years, particularly in medication regarding their antibacterial properties which had a lot of active components such as saponin and anthraquinone. Methicillin-resistant Staphylococcus aureus has emerged as one of the most important human pathogens causing nosocomial infections and became the first priority as a consequence of their resistancy. The purpose of this study is to determine the minimum levels of ethanol extract of leaves and Aloe Vera gel that can inhibit MRSA and find out whether there are differences in antibacterial power between of ethanol extract of leaves and Aloe Vera gel. Methods: Ten isolates of MRSA were investigated for their sensitivity to Aloe vera leaf and gel extract using the serial dilution method by doing two times repetition and statistically analyzed according to t-test method. Result: The result shows that the Minimum Inhibitory Concentration of Aloe vera leaf and gel extract each located at a concentration of 25% and 12,5%. There is a significant difference in antibacterial power between the ethanol extract of leaves and Aloe Vera gel in inhibiting MRSA with MIC values of 25% and 12.5% Conclusion: There was a difference between Aloe vera leaf and gel extract antibacterial activity, where the gel extract is more effective than leaf extract.
Antibacterial effect of clove (Eugenia aromaticum) oil extracted from clove cigarettes towards Streptococcus mutans Jumali, Ahmad Alif Wazir; Satari, Mieke Hemiawati; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 25, No 1 (2013): March 2013
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.426 KB) | DOI: 10.24198/pjd.vol25no1.15428

Abstract

Streptococcus mutans is a commensal bacteria of the oral cavity, particularly found in dental plaque attached to the tooth surface, and can also found in the saliva, buccal mucosa, tongue, and the gingival sulcus. Clove cigarette contains the clove oil used worldwide as a herbal remedy for a variety of health disorders due to its antibacterial, antifungal, and antiviral properties. The purpose of this study was to determined the antibacterial effect of the clove oil contained in the clove cigarette towards Streptococcus mutans as a cariogenic bacteria. The research was an experimental laboratory, which tested the sensitivity of the Streptococcus mutans bacteria taken from the saliva of 10 clove cigarette smokers towards the clove oil extract of the clove cigarette in-vitro by using the Kirby-Bauer method. The study showed that the clove oil extract of the clove cigarette which contained eugenol has antibacterial towards the growth of Streptococcus mutans, and there was a difference of the antibacterial activity between clove oil extracted from minced and combusted clove cigarette. The conclusion of this study was the clove oil extracted from minced clove cigarette had a better antibacterial effect than the combusted clove cigarette.
Antibacterial effect of pineapple (Ananas comosus) extract towards Staphylococcus aureus Loon, Yong Ker; Satari, Mieke Hemiawati; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 30, No 1 (2018): March 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.911 KB) | DOI: 10.24198/pjd.vol30no1.16099

Abstract

Introduction: Staphylococcus aureus is one of the important medical pathogens which have been recognised for many years as a remedyfor a wide case of oral infections. Nowadays, the use of herbal remedy for reducing bacteria in the oral cavity has been implemented widely due to thefewer side effects. Therefore, researchershave been findingwaysto use pineapple in dentistry to prevent many cases of oral diseases. The purpose of this study was to prove that pineapple extract indifferent concentration had the potential as an antibacterial agent towards Staphylococcus aureus. Methods: The study was an experimental laboratory conducted by determining the minimum inhibitory concentration of pineapple (Ananas comosus) with thetwo-fold serial dilution methods. Results:The The Minimum Inhibitory Concentration (MIC) of the pineapple extract was 1.56%-0.78%. Conclusion: The pineapple extract had anantibacterial effect towards Staphylococcus aureus due to the bromelain compound and its phytochemical factor such as Vitamin C and flavonoid. 
Denture adhesive antifungal potency towards the growth of Candida Albicans Dewi, Siti Utami; Sumarsongko, Taufik; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November 2011
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2234.583 KB) | DOI: 10.24198/pjd.vol23no3.14036

Abstract

Denture adhesive is a device that applied to the base of a denture before the denture is inserted into the mouth. The device used to improve denture retention and stabilization. It was thought that added antifungal agent to denture adhesive might be an effective way to inhibit the growth of Candida albicans and prevent denture stomatitis.The study was performed as an experimental laboratory study by an observed antifungal test of denture adhesive against Candida albicans growth using Kirby Bauer diffusion agar method with 5 samples and 3-time repetitions. The conclusion of this study was that the antifungal agent in denture adhesive containing poly (methylvinylether/maleic acid), sodium-calcium mixed partial salt and propyl hydroxybenzoate and denture adhesive containing sodium carboxymethylcellulose couldn't inhibit the growth of Candida albicans.
The decreased of Streptococcus Mutans growth after topical application of phosphopeptide amorphous calcium phosphate paste Araf, Tika Faradina; Gartika, Meirina; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.087 KB) | DOI: 10.24198/pjd.vol23no2.14023

Abstract

Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) paste is a topical application substance that consisted of a series of milk derivative peptide as a result of phosphorylation and has an antibacterial activity. The objective of this research was to find out the difference of Streptococcus mutans growth before and after CPP-ACP paste given topically to child's teeth. The method of the research was a quasi-experiment. Research samples were 10 students of MI Al Falah Islamic Boarding School, Jatinangor, West Jawa Indonesia and collected with purposive sampling technique. This research used dental plaque from child's teeth before and after applicated by CPP-ACP paste. The plaque was cultivated in selective media Tryptone Yeast Cysteine Sucrose Bacitracin (TYCSB) with repeated twice. Streptococcus mutans colony in TYCSB were counted by Stuart colony counter and statistically analyzed based on paired t-test. The results showed the average of Streptococcus mutans growth before applicated CPP-ACP paste was 57.05, whereas after applicated CPP-ACP paste for 1 days was 9.4; for 3 days was 2.85, and for 14 days was 1.7 colony. The research concluded that there was a decrease of Streptococcus mutans growth in isolate plaque after CPP-ACP paste topically given to child's teeth.
Minimum inhibition concentration and anti-fungal contact time of quaternary ammonium and ethylenediaminetetraacetic acid (EDTA) mixture towards Candida Albicans isolate Yunita, Elizabeth; Hardjawinata, Karlina; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 20, No 2 (2008): July 2008
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.788 KB) | DOI: 10.24198/pjd.vol20no2.14140

Abstract

The aim of this study is to determine the Minimum Inhibitory Concentration (MIC) and the exposure time of the combination of quaternary ammonium compound with EDTA towards Candida albicans isolates from the 5 upper acrylic removable complete dentures. This experimental laboratory study was conducted based on a serial dilution of the combination of quaternary ammonium compound with EDTA towards Candida albicans in 3 replications and statistically analyzed according to Kruskal-Wallis method. The result showed that the MIC of the combination of quaternary ammonium compound with EDTA towards Candida albicans was in 1/8000 concentration with minimum 8 hours exposure time. This study concluded that the combination of quaternary ammonium compound with EDTA had an antifungal activity towards Candida albicans at 1/8000 concentration in 8 hours exposure time.
Anti-fungal capacity of Saga leaf (Abrus Precatorius L) towards Candida Albicans testing NA, Muslim Paranto; Harjadinata, Karlina; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 19, No 2 (2007): July 2007
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.535 KB) | DOI: 10.24198/pjd.vol19no2.14180

Abstract

The aim of this study was to evaluate the antifungal effect, by determining the Minimum Inhibitory Concentration (MIC) of saga leave extract against Candida albicans, as a causal of oral candidiasis. The saga left was extracted following maceration method. Candida albicans, as a tested fungal was isolated from patients at the Oral and Dental Hospital Faculty of Dentistry Universitas Padjadjaran. They were cultured on Sabouraud Agar plate, incubated at 37°C for 18-24 hours. The isolation and the identification of Candida albicans were based on colonial morphology and the fermentation of glucose, maltose, saccharose, dan lactose at the Microbiology Laboratory. This experimental laboratory study was conducted according to a serial dilution method from 16 mg/ml up to 0,25 mg/ml of saga leave extract with two-fold dilution in three repetitions. The result showed that the Saga leaves extract to be able to inhibit Candida albicans at minimum 2 mg/ml concentration. As a conclusion, the concentration of 2 mg/ml or more of saga leave extract has antifungal effect against Candida albicans.
Bacteriology in Diagnosing Oral Odontogenic Infections: A Scoping Review Hazhiyyah, Nabila Ghassania; Dewi, Warta; Balafif, Felisha Febriane
Journal of Syiah Kuala Dentistry Society Vol 8, No 2 (2023): December 2023
Publisher : Dentistry Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jds.v8i2.32710

Abstract

Background: Odontogenic infection is an invasion of pathogenic microbes that originate from the tooth or its supporting structures and can spread to the surrounding tissue. Bacteriological examination to support the diagnosis. Objective: This study aims to discuss the role of bacteriological examination in helping to diagnose odontogenic infections. Method: Scoping review research type. Search for research gaps using the PCC strategy. PubMed, Scopus, ScienceDirect and Google Scholar databases were used to search for articles by entering keywords using Boolean Operators. Reporting of scoping review results using PRISMA-ScR guidelines. Results: Oral Streptococcus species in caries samples included S. mutans, S. salivarius, S. mitis and S. sanguis with the dominant bacteria being S. mutans. Results: The highest bacterial prevalence of odontogenic infections was Staphylococcus bacteria with high sensitivity to penicillin antibiotics. Periodontal pocket samples showed the highest differences in bacterial prevalence from the three articles, including Staphylococcus aureus, Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans. Enterococcus was the dominant bacteria in two articles with samples taken from root canals. Odontogenic abscess samples showed that Gram-positive bacteria were more dominant than Gram-negative bacteria, which were the most common in the culture results of abscess patients. The spread of odontogenic infections in the facial space shows that Methicillin resistant Staphylococcus aureus (MRSA) strains are the dominant bacteria found. Conclusion: Bacteriological examination should be carried out to show the dominant bacteria that cause odontogenic infections, so that it has benefits to support the diagnosis and treatment plan by the dentist