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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Omni-Akuatika Aquacultura Indonesiana Journal of Aquaculture and Fish Health Jurnal Lemuru : Jurnal Ilmu Perikanan dan Kelautan Indonesia Jurnal Komunitas: Jurnal Pengabidian Kepada Masyarakat Molekul: Jurnal Ilmiah Kimia Indonesian Journal of Forestry Research
Norman Arie Prayogo, Norman Arie
Depatrment of Aquatic Resources Management Faculty of Fisheries and Marine Science, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Public Health Veterinary Other

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Effects of Photoperiods on the Cloned GtH Genes in Hard-Lipped Barb (Osteochillus hasseltii) Prayogo, Norman Arie; Pramono, Taufik Budi; Siregar, Asrul Sahri; Sukardi, Purnama; Kawaichi, Masashi
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.3.1100

Abstract

Photoperiod is one of the factors that regulate endocrine gland hormone production necessary for gonadal growth and development, gametogenesis, and reproductive cycles in fish. However, studies on the influence of photoperiod on tropical fishes are still limited. Therefore, this study investigated the effects of photoperiod on the hard-lipped barb, particularly the gene expression of its cloned gonadotropin hormones I (GtH-I) and II (GtH-II). The experiment was designed using three treatments: 14L:10D (control), 8L:16D (short photoperiod), and 18L:6D (long photoperiod). Four aquariums with nine fish per tank were used as replicates. The fish were maintained under these photoperiod treatments for eight weeks. Pituitary activity was observed by measuring GtH gene expression. The length of cDNA GtH-I was 222 bp, and the cDNA GtH-II was 354 bp. The GtH-I precursor encoded by the cDNA consisted of 67 amino acids, including the mature peptide. The level of GtH gene expression significantly increased as longer photoperiods were applied. The results indicated that photoperiod length affects the reproductive performance of the hard-lipped barb.
Spirulina platensis Diet for Milkfish, Chanos chanos, Larvae Sukardi, Purnama; Yansah, Noprie; Winanto, Tjahyo; Marnani, Sri; Prayogo, Norman Arie; Harisam, Taufan; Sudaryono, Agung
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.841 KB) | DOI: 10.11598/btb.2019.26.3.1103

Abstract

In aquaculture, Spirulina platensis is used as a feed supplement as it contains the amino acid phenylalanine. This study was conducted to evaluate the differences in the effects of spirulina-based microcapsules and commercial diets on the absolute, daily, and specific growth rates, as well as the survival rates, of milkfish larvae. The larvae were fed with Spirulina platensis as a core diet in microcapsules with different matrix (wall) compositions. The first capsule wall consisted of gelatin and fish oil, while the second capsule wall contained gelatin, fish oil, and whole egg. The control group was fed a commercial diet. A total of 1,200 larvae were used in this experiment using a recirculating system. The experiment was conducted over 42 days of culture. The larvae were fed three times per day, and the feed amount was increased regularly in accordance with larval growth. The results showed that the effects of both spirulina-based microcapsule diets on absolute growth rate (AGR), specific growth rate (SGR), and average daily growth rate (ADGR) of Chanos chanos larvae were comparable to those fed a commercial diet. The survival rates were 80.6 ± 11.17% for larvae fed Spirulina platensis with a gelatin and fish oil wall, 84.6 ± 8.44% for those fed Spirulina platensis with a gelatin, fish oil, and whole egg wall, and 83.8 ± 16.50% for those fed the commercial diet. This study demonstrated that spirulina-based microcapsules had similar effects to commercial feed on the growth of milkfish larvae, indicating that this diet could potentially replace commercial feed.
Genetics Identification of Sea Holly (Acanthus ilicifolius) Through Dna Barcoding From Coastal Cilacap, Central Java, Indonesia Harisam, Taufan; Haryono, Florencius Eko Dwi; Marhaeni, Bintang; Amron, Amron; Prayogo, Norman Arie; Sukardi, Purnama
BIOTROPIA Vol. 27 No. 1 (2020): BIOTROPIA Vol. 27 No. 1 April 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2020.27.1.1105

Abstract

Sea holly (Acanthus ilicifolius) is an important true mangrove species commonly growing in wetlands at river mouths and coastal areas. Very limited information is available on the molecular taxonomy of sea holly growing along the coasts of Cilacap, Central Java, Indonesia. The present study aimed to identify sea holly in coastal Cilacap and to produce a reference library on the molecular characteristics of the species. The recently recorded species were utilized for the barcoding investigation. Genetic identification was evaluated using the rbcL and matK genes. Young leaf samples of A. ilicifolius were collected for DNA extraction, isolation, and amplification using rbcL and matK primers. The length of the rbcL gene was 608 bp and the matK gene was 970 bp. The evolutionary history was constructed using the Neighbor-Joining method. The barcode sequences of rbcL and matK were analyzed using BLAST and MULTALIN. The sequences were also submitted to NCBI. Genus Acanthus (Acanthaceae) and other genera were clustered in the same clade with high bootstrap values. The results indicated that the rbcL and matK loci cannot be used for species differentiation within Acanthus; however, these genes can be used to distinguish the genus level within Acanthaceae.
Co-Authors Agung Sudaryono Agus Nuryanto Amron Amron, Amron Ardiyanto, Anjalli Tasha Arif Mahdiana, Arif Asrul Sahri Siregar Bessho, Yasumasa Cahyo, Tri Nur Endang Hilmi Fianjani, Ajeng Sekar Fitriadi, Ren Harisam, Taufan Harisman, Taufan Haryono, Florencius Eko Dwi Junaidi, Teuku Kurniawati, Mutiara Ayu Lilik Kartika Sari, Lilik Kartika Marhaeni, Bintang Masashi Kawaichi, Masashi Muhamad Baedowi Muslih Muslih Nuning Vita Hidayati Pramono, Taufik Budi Purnama Sukardi Putra, Rifky Raihady Danu Rose Dewi Rosita, Rita Eka Sari, Fitra Amalia Sesilia Rani Samudra Sri Marnani SRI RAHAYU Syakuri, Hamdan Taufik Budhi Pramono Winanto, Tjahyo Yansah, Noprie