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Journal : MPSJ

Analisis Kandungan Kimia Dan Penetapan Kadar Polifenol Daun Lerak (Sapindus rarak DC) Dengan Metode Spektrofotometri UV-Vis Salim, Medina; Maryam, St.; Muflihunna, A.
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 3 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i3.290

Abstract

MEDINA SALIM. Chemical Content Analysis and Determination of Lerak Leaf Polyphenol Content (Sapindus rarak DC) by UV-Vis Spectrophotometry Method (Guided by St. Maryam and A. Muflihunna). Lerak contains polyphenolic compounds that reduce blood sugar levels. This study aims to determine the chemical content contained in lerak leaf extract (Sapindus rarak DC) and find out how much polyphenol compounds in the sample. The samples were macerated for 3 days using 96% ethanol solvent, the extraction process was carried out until obtaining an extract yield of 9.323 grams, then two tests were carried out, namely qualitative and quantitative, The qualitative test uses a wet reagent while the quantitative test uses the UV-Vis spectrophotometry method. The maximum wavelength used was 768 nm with gallic acid as a comparison. Based on the study, the results of ethanol extract of lerak leaves (Sapindus rarak DC) contain secondary metabolites, namely saponins, steroids, polyphenols and tannins and the result of polyphenol content is 21,168 mgGAE/g.
analisis kadar analisis kadar senyawa tanin pada teh daun tanaman kakao (theobroma cacao L.) secara spektrofotometri UV-Vis arifin, nurrizky afiah; Suhaenah, Asriani; Maryam, St.
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 3 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i3.291

Abstract

Cocoa leaves (Theobroma cacao L.) contain secondary metabolite compounds, namely flavonoids, saponins, tannins, theobromine, caffeine, anthocyanins, leucoanthocyanins and catechol. This research aims to determine the levels of tannin compounds contained in tea preparations from the leaves of the cocoa plant (Theobroma cacao L.) based on a comparison of growing places, namely Wajo, Jeneponto and Malino. The research was carried out qualitatively using wet reagents and quantitatively using the UV-Vis spectrophotometric method. Based on the qualitative test results, FeCl3 1% reagent produces a green solution blackish, the gelatin test produces precipitation, while the potassium ferricyanide and ammonia reagent produces a dark brown color, these results indicate that cocoa leaves contain tannin compounds, for the quantitative test it is measured using UVVis spectrophotometry at a maximum wavelength of 772 nm with a reference, namely gallic acid. . The results obtained for the Wajo area were 1,618mgGAE/g, Jeneponto 2,334mgGAE/g, and Malino 1,030mgGAE/g, based on these data it can be concluded that cocoa leaves contain tannin compounds and the highest levels are in the Jeneponto area.