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All Journal Pro-Life Paspalum: Jurnal Ilmiah Pertanian
Ervinda Yuliatin
Program Studi Biologi, Jurusan Biologi, FMIPA Universitas Mulawarman, Jl. Barong Tongkok No.4 Kampus Gunung Kelua, Samarinda, Indonesia 75123
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Respon Pertumbuhan Protocorm Like Bodies (PLB) Anggrek Tebu (Grammatophyllum scriptum Blume) terhadap Modifikasi Media Murashige and Skoog (MS) secara In Vitro Ratna Kusuma; Samsurianto Samsurianto; Wafif Azizah; Ervinda Yuliatin
Paspalum: Jurnal Ilmiah Pertanian Vol 11, No 1 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35138/paspalum.v11i1.538

Abstract

Anggrek Tebu (Grammatophyllum scriptum) propagation can be improved optimally by adding plant growth regulators such as BAP and NAA through in vitro technique. Murashige and Skoog (MS) medium is an essential medium for plant tissue growth that needs another complex mineral and vitamin to develop the protocorm-like bodies (PBL) of Anggrek Tebu. Hence, this study aimed to gain the appropriate composition of modified MS by adding BAP and various coconut water concentrations and to evaluate their effect on PBL of Anggrek Tebu growth (budding time, number of buds, and leaves). The research was conducted using a completely randomized design with two factorials such as modified MS medium (MS (M1), ½ MS (M2), and ¼ MS (M3))and coconut water concentration (0 ml/L (C0), 100 ml/L (C1), 200 ml /L (C2), and 300 ml/L (C3)) with triplication. The result showed that the fastest budding time was remarkable growth from M2C1 offered the bud at three weeks after planting (WAP). In the number of budding, M3C2 showed excellent results and significantly increased the budding growth at 8.33 ± 0.88  in 10 WAP. Meanwhile, the highest leave number was M2C1 at 23.00 ± 3.60, followed by M2C2 and M3C2, respectively, at 20.67 ± 1.45 and 20.67 ± 1.33. This result indicated that modifying MS medium composition by adding the coconut water could assist the PBL of Anggrek Tebu growth in a specific concentration. 
Karakteristik dan Potensi Bakteri Rhizosfer Penghasil Indole Acetic Acid yang Berasosiasi dengan Tanaman Kopi (Coffea spp.) Ervinda Yuliatin; Mamluatul Faizah
Pro-Life Vol. 10 No. 2 (2023): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v10i2.4899

Abstract

Coffee (Coffea spp.) consumption increased faster than five years ago. However, coffee production availability declined due to the limited field, soil nutrient deficiency, and chemical fertilizer. The aimed research was to obtain a bacteria-producing IAA hormone isolate and to evaluate its density and diversity in improving soil quality and fertility for coffee growth. The rhizosphere soil samples were collected around the coffee plantation in UBF at Sumberwangi, Malang. The sample was isolated using serial dilution on 0.85% NaCl and spread on a Tryptic soy agar medium. The result revealed that the total number of bacteria-producing IAA was 36 isolates, and two isolates (TAS1.2 and TAS 2.6) had higher IAA hormone production at 60 ppm. The selected bacteria can be optimized for a candidate of bacteria-based fertilizer coffee production. Keywords: IAA, density, diversity index, PGPR
Co-Authors Faizah, Mamluatul Ratna Kusuma Samsurianto Wafif Azizah