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Probiotic Concentrations Effect on Density and Compotition of Infusoria Grown on Banana Leaf Substrate (Musa paradisiaca) Laras Puput Insanni; Sucahyo Sucahyo; Desti Christian Cahyaningrum
Jurnal Ilmiah Sains Volume 22 Nomor 2, Oktober 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (798.828 KB) | DOI: 10.35799/jis.v22i2.39785

Abstract

This study aims to determine the effect of probiotic concentration on the density and composition of infusoria grown on banana leaf substrate (Musa paradisiaca). This research is an experimental study in a one-factor Completely Randomized Design (CRD) with five levels of treatment, namely the probiotic concentration 0 ml/l,  0,5 ml/l, 1 ml/l,  2 ml/l and 4 ml/l with 4 repetitions for each treatment. Observations were made on days 2, 4, 6, 8, 10, 12, and 14 to obtain data in the form of infusoria density, infusoria species diversity and the number of individuals per species. These data were used to determine the density, diversity index (H'), dominance index, and the composition of infusoria species in each treatment. The results showed that the concentration of probiotics had a significant effect on the density of insuffocia (sig 0.010) based on One Way ANOVA analysis. The infusoria diversity index was included in the low category in all treatments. In line with these results, the dominance index in all treatments was in the high category. The results showed that Paramecium sp dominated the composition of the infusoria at all concentrations. The probiotic concentration was 0,5ml/l; 1ml/l; stimulated the growth of Chlorococcum and was replaced by Trichocerca tenuior populations at concentrations of 2 ml/l and 4 ml/l.Keywords: Antioxidant; chromatography profil; DPPH method; white frangipaniPengaruh Konsentrasi Probiotik Terhadap Kepadatan dan Komposisi Infusoria yang Ditumbuhkan pada Substrat Daun Pisang (Musa paradisiaca)ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh konsentrasi probiotik terhadap kepadatan dan komposisi infusoria yang ditumbuhkan pada subtrat daun pisang (Musa paradisiaca). Penelitian ini merupakan penelitian eksperimental Rancangan Acak Lengkap (RAL) satu faktor dengan lima taraf perlakuan yaitu konsentrasi probiotik 0 ml/l, 0,5 ml/l, 1 ml/l, 2 ml/l,  dan 4 ml/l dengan pengulangan sebanyak 4 kali untuk setiap perlakuan.  Pengamatan dilakukan pada hari ke 2, 4, 6, 8, 10, 12, dan 14 untuk memperoleh data berupa kepadatan infusoria, keanekeragaman spesies infusoria beserta jumlah individu per spesies. Data tersebut digunakan untuk mengetahui kepadatan, indeks keanekaragaman (H’), indeks dominansi, serta komposisi spesies infusoria pada setiap perlakuan. Hasil penelitian menunjukkan bahwa konsentrasi probiotik berpengaruh signifikan terhadap kepadatan insufosia (sig 0,010) berdasarkan analisis One Way ANOVA. Indeks keanekaragaman infusoria termasuk dalam kategori rendah pada semua perlakuan. Sejalan dengan hasil tersebut, indeks dominansi pada semua perlakuan berada dalam kategori tinggi. Hasil penelitian menunjukkan bahwa Paramecium sp mendominasi komposisi infusoria pada semua konsentrasi probiotik. Konsentrasi probiotik sebesar 0,5 ml/l,  1 ml/l memicu pertumbuhan Chlorococcum dan digantikan populasi Trichocerca tenuior pada konsentrasi 2 ml/l dan 4 ml/l. Kata kunci: dominansi, infusoria, keanekaragaman, probiotik 
Effect of Feeding Fermented Catfish Pellets with Probiotics on Population Growth of Water Fleas (Daphnia Magna) Cantika Glodia; Sucahyo
Britain International of Exact Sciences (BIoEx) Journal Vol 4 No 3 (2022): Britain International of Exact Sciences Journal, September
Publisher : Britain International for Academic Research (BIAR) Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33258/bioex.v4i3.736

Abstract

D. magna as feed has advantages: easy to digest, the size is in accordance with the larval mouth opening, has 42.65% protein in the form of nutrition, contains a number of digestive enzymes, does not degrade water quality and can be cultivated in bulk. In this study, commercial catfish pellets fermented with probiotics were used as feed for D. magna. The probiotic that is often used in aquaculture is EM4 (Effective Microorganism 4). EM4 is able to play a role in increasing protein levels and with the right dose, EM4 becomes the main determining factor in growth productivity. The purpose of this study was to determine the good duration of EM4 fermentation in population growth of D. magna. This study is an experimental study (CRD), using D. magna as a test animal, with 5 different treatments. The results of the density of D. magna which were given different treatments gave different results. The highest density was found in treatment 3 and the treatment that had the lowest density was in treatment 5. The results of the Nonparametric test (Friedman-Test) showed significant results (P=0.00<0.05). Significantly different treatments were in treatment 3 and 5, while treatment 2 and treatment 4 had no significant difference. The results of the study the highest specific growth rate obtained was in treatment 4 of 0.223%/day, then followed by treatment 3 of 0.211%/day, while other treatments were relatively lower. Thus, feeding in the form of catfish pellets fermented with EM4 on the population growth of Daphnia magna gave a significant effect on treatment 3 and treatment 5 with the highest specific growth rate in treatment 4.
PENGARUH Mangan (Mn) TERHADAP OKSIDASI KROMIUM TRIVALEN DAN PERTUMBUHAN Tagetes sp. Margareta Prameswati; Sri Kasmiyati; Sucahyo Sucahyo
BERITA BIOLOGI Vol 21, No 3 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4190

Abstract

Chromium can be found in 2 oxidation states, trivalent chromium (Cr3+) and hexavalent chromium (Cr6+) in environment. Trivalent chromium can be oxidized by manganese oxide to hexavalent chromium (Cr6+). The aim of the study was to determine the effect of Mn on the oxidation of Cr³⁺and plant growth of Tagetes. The study was conducted experimentally using a completely randomized design. The Tagetes plants grown in media with 2 treatment factors, namely Cr3+ (CrCl3) with 3 concentrations of 0, 100 and 500 mg/l) and Mn (MnSO4) with 4 concentrations of 0, 1, 5 and 10 mg/l. The plant growth and Cr6+ content in roots and shoots were measured to determine the effect and to detect the occurrence of Cr3+ oxidation. The addition of Mn in the media of Tagetes containing Cr3+ increased the Cr3+ oxidation process, indicated by the detection of Cr6+ in the roots and shoots of Tagetes. The highest Cr6+ content was found in the roots and shoots of Tagetes sp. treated with Cr3+ 500 mg/l and Mn 10 mg/l at 0.059 and 0.035 g/l, respectively. The interaction of Cr3+ and Mn treatments significantly affected the growth of Tagetes plants. Cr3+ and Mn at high concentrations increased plant height, root length, root and shoot dry weight. The interaction of Cr3+ and Mn at high concentrations inhibited the increase in the number of leaves of Tagetes.
Efek Probiotik EM4 pada Sistem Akuaponik terhadap Pertumbuhan, dan Mortalitas Ikan Guppy, serta Pertumbuhan Tanaman Mint Dimas Andre Adi Wibowo; Sucahyo Sucahyo; Djohan Djohan
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 1: March 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i1.19330

Abstract

Penggunaan Air Limbah Rendaman Kedelai yang Difermentasi dalam Budidaya Kutu Air (Daphnia magna) Rahel Rosana Pramudiva; Sucahyo Sucahyo
Buletin Anatomi dan Fisiologi Volume 8, Nomor 1, Tahun 2023
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.8.1.2023.1-9

Abstract

Ketersediaan air limbah rendaman kedelai yang melimpah dapat dijadikan sebagai pakan alternatif bagi D. magna. Peningkatan nutrisi dapat dilakukan melalui penambahan probiotik EM4. Tujuan penelitian ini adalah untuk mengetahui pengaruh perbedaan konsentrasi pemberian pakan air limbah rendaman kedelai terfermentasi dan periode waktu terhadap pertumbuhan D. magna. Penelitian dilaksanakan secara eksperimental di Laboratorium Akuakultur, Fakultas Biologi UKSW. Hasil pengamatan pertumbuhan D. magna tertinggi ditunjukkan oleh P3 (pakan terfermentasi 10 ml) sebanyak 922 ind/L, sedangkan perlakuan yang tidak mengalami pertumbuhan berarti ditunjukkan oleh P6 (pakan terfermentasi 80 ml) dan P1 sebagai kontrol. Konsentrasi terbaik ditunjukkan oleh P3 (pakan terfermentasi 10 ml). Pemberian air limbah rendaman kedelai terfermentasi sebagai asupan pakan D. magna lebih efektif dibandingkan dengan pakan tanpa fermentasi.The abundant availability of soy soaking wastewater could be used as an alternative feed for D. magna. Improving the feed’s nutrition could be done through by adding EM4 probiotics. This study aimed to determine the impact of differences in the concentration of fermented soy soaking wastewater feed and the growth period on the growth of D. magna.  The study was conducted experimentally in the Aquaculture Laboratory, Faculty of Biology UKSW. The observation revealed that the highest D. magna growth was demonstrated by P3 (10 ml of fermented feed) with 922 ind/L. In comparison, the treatment that did not experience significant growth was shown by P6 (80 ml fermented feed) with  P1 as the control.  The best feed concentration was  P3 (10 ml fermented feed). To conclude, the provision of fermented soy soaking wastewater as a D. magna’s feed intake was more effective compared to non-fermented feed intake.
The Effect Of Business Risk, and Financial Risk on Profitability in Mining Sector Companies Listed on The Indonesia Stock Exchange (IDX) for The 2018-2021 Period Jesyca Ireyne Duru; Usil Sis Sucahyo
Return : Study of Management, Economic and Bussines Vol. 2 No. 9 (2023): Return : Study of Management, Economic And Bussines
Publisher : PT. Publikasiku Academic Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57096/return.v2i9.153

Abstract

The purpose of this study is to determine how much influence Total Asset Turnover, Debt to Equity Ratio, has on Return On Equity in mining sub-sector companies listed on the Indonesia Stock Exchange (IDX) for the 2018-2021 period. The researcaes take a total of 60 Sample is done by purposive sampling technique. This research uses quantitative methods with descriptive analysis techniques using SPSS version 20 software.the data used is secondary data and obtained through the official website of the Indonesia Stock Exchange (IDX) in the form of annual financial statements (www.idx.co.id). Through this research, it was found that Total Asset Turnover had a significant positive effect on Return On Equity in mining sub-sector companies. Meanwhile, the Debt to Equity Ratio has a significant negative effect on Return On Equity in mining sub-sector companies.
Growth Response of Sorghum bicolor (L.) Moench. Cultivars to Trivalent Chromium Stress Sri Kasmiyati; Santosa Santosa; Irfan Dwidja Priyambada; Kumala Dewi; Sucahyo Sucahyo; Rintawati Sandradewi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5178

Abstract

One of heavy metal pollutants in the soil that can be absorbed by the sorghum is chromium (Cr). The study was conducted to determine the growth response of Cr3+ stress of sorghum cultivars. Two chemical compounds Cr3+ and 3 level concentrations were exposed to sorghum cultivars. The research was conducted in two separate experiments i.e. during seed germination and early seedling development stages. The parameters measured were radicle/root length, seedling length, fresh weight, dry weight, and stress tolerance index (STI) value. The results showed that Cr3+ either in form of CrCl3 or KCr(SO4)2 significantly reduced the seedling growth of sorghum cultivars. The growth responses of sorghum cultivars toward Cr3+ stress showed differences both on stage of the germination and early seedling. Based on the average of STI value, four sorghum cultivars (Badik, Keris, Keris M3 and Numbu) were classified as very strong tolerant, 4 cultivars (Hegari, Mandau, Sangkur and Gambela) were categorized as moderate tolerant, two cultivars (UPCA and Selayer) were weak tolerant, and 2 cultivars (Kawali and Batari) were sensitive ones, under stress condition of Cr3+.The results of this study are expected to provide the scientific basis of the physiological and tolerance responses of sorghum cultivars toward Cr3+ stress condition.How to CiteKasmiyati, S., Santosa, S., Priyambada, I., Dewi, K., Sucahyo, S., Sandradewi, R. (2016). Growth Response of Sorghum bicolor (L.) Moench. Cultivars to Trivalent Chromium Stress. Biosaintifika: Journal of Biology Biology Education, 8(1), 71-84.
DETEKSI CEKAMAN OKSIDATIF AKIBAT TOKSISITAS KROM PADASonchus oleraceus L. MELALUI PENENTUAN SPESIES OKSIGEN REAKTIF SECARA SPEKTROFOTOMETRI DAN HISTOKIMIA Sri Kasmiyati; - Sucahyo
Agric Vol. 26 No. 1 (2014)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2014.v26.i1.p85-98

Abstract

Increased production of reactive oxygen species or ROS is one of the common responses to a wide range of biotic and abiotic stresses. Increased production of ROS is outstripping endogenous antioxidant defense systems has been referred to as oxidative stress. Heavy metals are known to initiate ROS generation which is implicated as a oxidative stress. Cr is a toxic heavy metal that can generate ROS like H2O2 and O2 - which cause oxidative stress. In this study, chromium toxicity was studied to detect the oxidative stress on Sonchus oleraceus weed plants by the detection of superoxide anion and H2O2. Superoxide anion was detected by staining techniques with nitroblue tetrazolium (NBT) and hydrogen peroxide by Diaminobenzidine tetrahydrochloride (DAB) staining. Results indicated that the plants were grown in sand media generate the highest (0.89 A/g FW and 3.23 mol/g FW) than in soil media (0.23 A/g FW and 2.11 mol/g FW) superoxide anion (*O- 2) and H2O2 and soil containing textile sludge (0.18 A/g FW and 2.66 mol/g FW), respectively. At application of 10 mg Cr6+/L and 250 mg Cr3+/L, the production of *O- 2 and H2O2 in leaves of sonchus plants were significantly increased compared with the control plants. The highest production of H2O2 and *O- 2 were showed in the leaves of sonchus plants grown in sand media with Cr6+ application. In this study, either Cr3+ or Cr6+ caused oxidative stress in Sonchus oleraceus weed plants. The result also showed that sonchus plants esposed to toxic Cr can suffer from oxidative stress leading to reduction of its fresh and dry plants biomass. NBT and DAB in an appropriate probe and significant value for monitoring the formation of *O- 2 and H2O2 in plants.
Biology learning innovation in the water pollution sub material based on sustainable development goals (SDGs) using the problem-based learning Nabila Nabila; Marisa Christina Tapilouw; Sucahyo Sucahyo
BIO-INOVED : Jurnal Biologi-Inovasi Pendidikan Vol 5, No 3 (2023): October 2023
Publisher : Master Program of Biology Education, Universitas Lambung Mangkurat, Banjarmasin, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/bino.v5i3.16718

Abstract

Sustainable Development Goals (SDGs) are a global issue initiated by the United Nations. One goal of SDGs is "clean water and sanitation" regarding water pollution issues. This research aims to develop learning media on the material "Water pollution" in the form of 360° Videos and water purification tools to introduce Sustainable Development Goals (SDGs), determine the effectiveness of learning innovations in improving learning outcomes, and also determine the feasibility of 360° Video learning media and water purification tools. This research is developing learning media with 4D models (Define, Design, Development, and Disseminate). The media developed are 360° Video and water purification devices. The value of 360° video validation is 93.3%, and the water purification device is 91.25% with the very valid category. The learning media developed was able to improve learning outcomes with an N-Gain value of 0.508 in the medium category. The value of effectiveness is 80.66% with the effective category. 360° Videos and water purification tools improved student learning outcomes, SDGS understanding of the clean water and sanitation goals, and student enthusiasm.Abstrak Sustainable Development Goals (SDGs)  merupakan isu global yang diinisiasi oleh PBB. Salah satu tujuan SDGs yaitu Clean water and Sanitation, berdasarkan masalah pencemaran air.  Tujuan penelitian ini yaitu untuk mengembangkan media pembelajaran pada materi “pencemaran air” dalam bentuk Video 360° dan alat penjernihan air untuk memperkenalkan Sustainable Development Goals (SDGs), dan menentukan efektivitas inovasi pembelajaran dan kelayakan Video 360° dan alat penjernihan air. Penelitian ini merupakan pengembangan media pembelajaran dengan model 4D (Define, Design, Development, dan Disseminate). Media yang dikembangkan yaitu Video 360° dan alat penjernihan air. Hasil validasi video 360 yaitu 93,3% dan alat penjernihan air yaitu 91,25% dengan kategori sangat valid. Media pembelajaran yang dikembangkan mampu meningkatkan hasil belajar dengan nilai N-Gain sebesar 0,508 pada kategori sedang.  Pada aspek efektivitas, mendpatkan nilai 80,66% dengan kategori efektif.  Video 360° dan alat penjernihan air mampu meningkatkan hasil belajar siswa, pemahaman SDGs Clean water and Sanitation dan antusiasme siswa.
Study of Gastropod Community Structure After Development of Tourism Activities on the Senjoyo River Marsahanda Astri Ramagita; Sucahyo Sucahyo; Desti Christian Cahyaningrum
Jurnal Natur Indonesia Vol 21, No 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.2.124-133

Abstract

It is thought that the development of the Senjoyo River as a tourist attraction in Semarang Regency will cause changes to the natural ecosystem in the area, one of which is changing the structure of the existing gastropod community. This research is a quantitative analysis research using a survey method that aims to examine the structure of the gastropod community after the development of tourism activities on the Senjoyo River. Gastropod samples were taken from 10 different observation stations along the Senjoyo River, which are located at 7˚22'23"S and 110˚31'37"E. Observation station locations were determined by the purposive random sampling method, based on tourism activity density. The result showed types of gastropods that were found in the Senjoyo River namely, Sulcospira testudinaria, Tarebia granifera, Brotia costula, Thiara scraba, and Melanoides tuberculata with clustered distribution patterns (Id>1). Statistical analysis showed that location affected gastropod abundance (Asymp. Sig of 0.043), with the highest abundance found at station seven (45,11 individuals/m2). So station seven, with sandy and muddy substrate conditions, moderate current speed (0.257 m/s), and rarely found human activity, is the most suitable location to support the survival of gastropods in the Senjoyo River. Furthermore, the gastropod community structure indicates environmental changes at observation stations nine and ten, the locations where the most activities and construction of tourism facilities were found. That was indicated by the low diversity (H’= 0.00-0.01) and evenness (E = 0.00) index of gastropods, and there is dominance by Sulcospira testudinaria (C= 0.98-1.00).