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Journal : Leuser Journal of Environmental Studies

Utilization of Empty Palm Fruit Bunches as a Carbon Source for Cellulase Production to Reduce Solid Waste from Palm Oil Amraini, Said Zul; Nazaris, Nazsha Nayyazsha; Andrio, David; Mardhiansyah, Muhammad; Helwani, Zuchra
Leuser Journal of Environmental Studies Vol. 1 No. 1 (2023): July 2023
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/ljes.v1i1.41

Abstract

Cellulase enzymes are widely used in textile, food, detergent, pulp and paper industries and biofuel, so the need for enzymes continues to increase every year. One of many biomass wastes found in Indonesia is empty fruit bunches (EFB) that can be used as a carbon source as a substitute for expensive pure cellulose (CMC) and Bacillus subtilis isolates. This study aims to obtain the optimum conditions the production of cellulase enzymes with variations in the pre-treatment of EFB and pH variations of the medium using Bacillus subtilis. Pre-treatment was carried out to hydrolysed lignocellulosic biomass was more easily and increased glucose levels which would enter the next production stage. Variations in pre-treatment were carried out by adding acids, bases and organosolv process, as well as variations in pH at 6.5; 7.0 and 7.5, respectively. Enzyme activity was calculated using the Nelson-Somogyi method. When using acid, the enzyme activity is 0.041, while using organosolv, it is obtained 0.057 each at pH 7. The results showed that the highest enzyme activity was obtained at a pH of 7.0 and a temperature of 40 ºC on EFB substrate pretreated with a base of 0.204 U/ml. These findings emphasize the potential benefits of using EFB waste as a substrate for cellulase enzyme production, by providing an alternative approach to decrease raw material expenses and mitigate environmental pollution.
Sustainable Biohydrogen Production from Palm Oil Mill Effluent: Effect of Hydraulic Retention Time in a Hybrid Anaerobic System Ahmad, Adrianto; Evelyn, Evelyn; Andrio, David; Avriliani, Dini; Dalil, M.; Hamzah, Amir
Leuser Journal of Environmental Studies Vol. 4 No. 1 (2026): April 2026
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/ljes.v4i1.379

Abstract

Currently, the world is facing two crises: a shortage of fossil fuels and global climate change. Climate change is linked to increased environmental damage from fossil fuel use and the effects of greenhouse gases. Therefore, it is important to achieve breakthroughs to develop alternative energy sources that can replace fossil fuels. One of these is biohydrogen, which plays an important role in future energy because it is environmentally friendly, renewable, and sustainable. In addition, Indonesia is the world's largest producer of palm oil, which naturally generates liquid waste. Using palm oil mill liquid waste to produce biohydrogen via an anaerobic hybrid bioreactor during the acidogenesis phase is the best solution to address environmental impacts while simultaneously providing a clean energy source. This research aims to produce biohydrogen from palm oil mill liquid waste. This was done using an anaerobic hybrid bioreactor during the acidogenesis phase, with hydraulic retention times of 6, 12, and 18 hours. The research results show that the best hydraulic retention time is 18 hours, with a VSS removal efficiency of 98% and biogas production of 5.0 L/day, yielding 64% biohydrogen.
Co-Authors Achmad Noerkhaerin Putra Adillah, Muhammad Iqbal Adinda Ryvania Abrir Adrianto Ahmad Adrianto Ahmad Agustinus Lamhot Viraro Aini, Keke Nur Alfi Aulia Rahman Amarullah Amarullah Amir Hamzah Amir Hamzah Andhini Getha Kusuma Anggraini, Melina Dwi Annisa Nur’alifa Anysha Lankari Halim Aprilya, Sondang Ita Aryo Sasmita Aurelia, Keyla Avriliani, Dini Azzah Fizda Bagus Anugrah Bahruddin Basopi, Hasbi Cahyani, Anugrah Christiani Widia BR Karo Dalil, M. Daniel Ilham Wahyudi Davidson Davidson DESSY NATALIA Dessy Natalia Dewi Fitria Dikki Awanda Dinil Fitri, Shafira Dzaki Furqoon Edward HS Eko Hendi Saputra, Eko Hendi Elvi Yenie, Elvi Ester Melinda EVELYN EVELYN Evelyn Evelyn Fachry Abda El Rahman Fadhila Fadhila Fadlilah, Lilya Irsianti Fatra, Waman Ferdy Ashari Syawal Fikri Julian Fri Murdiya, Fri Gusti Rahayu Hadi Purnama Putra, Hadi Purnama Hari Rionaldo Hasmad Setyadi Ikhwanul Ikhsan Indra Hidayatul Mubarah Intan Sri Rahmi, Intan Sri Ivnaini Andesgur Jecky Asmura Keke Nur Aini Kesni savitri Kesni Savitri Khalidazia Putri Kiki Ramadanti Kiki Ramadanti Laksono Trisnantoro Lita Darmayanti M Caesar Grendi M Kamal Syah Mahardika, Dewa Ayu Divia Pradhaswari Marisa Handajani Marisa Handajani Maulani, Annisa Maulidya, Syukri May Kristina Maya Ratna Sari Medio Novindra Putra Bayu Mindriany Syafila Mora Rahmayuanda Muhamad Ghifar Alharis Muhamad Gifari Muhammad Al- Hafizd Muhammad Azmi Muhammad Faisal Dharma Muhammad Mardhiansyah Muhammad Reza nadeak, Parade Naomi Ebinasari BR Sembiring Natalina, Riani Nazaris, Nazsha Nayyazsha Nazaris, Nazsha Nayyazsha Niko Ronaldo P. S Novalina Annisa Yudistira Nurul Annisa Pagattari, Hirda Nopma Parlaungan Hasibuan Prayoga Wiguna Priyambada, Gunadi Putri, Gustriana R. Rindu Permatasari Rahmadini Setianingsih Ramadhan, Gentha Ramida Elisa Kristiani Simanjuntak Reno Susanto Retna Julita Retno Dwi Pangastuti Rika Kristin Riri Shania Risma Nopita Risma Nopita Rizaldi Riski Irawan Rudhi Andreas Komang, Rudhi Andreas Sanny Amir Arasy Saputra, Muhammad Ramadhan Sari, Sunitha Shella Zahrawani Shifa Rayni Efendi Shinta Elystia Shinta Nurdiyanti Sinta Sinta Sisri Wantri Sanjaya Manalu Siti Aisyah Sukma Sri Rezeki Muria Sunitha Sari SUSANTO, RENO Syamsiar, Syamsiar Syarfi Daud tania, Nindy Tanjung, Munir Topan Herianto Trio Yudha Putra Ulfah Nurulita Ulva Seprizal Waman Fatra Wanda Suwarno Yohanes Yohanes Z Zulfansyah Zafira, Nesa Zuchra Helwani Zuchra Helwani, Zuchra