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All Journal MEKANIKA - JURNAL TEKNIK MESIN Makara Journal of Science
Prayoga, Indrajid
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Energy Engineering Mechanical Engineering

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Journal : Makara Journal of Science

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Construction and Characterization of Conductometric Biosensor for Determination of the Diazinon Concentration Prayoga, Indrajid; Mulyasuryani, Ani; Prasetyawan, Sasangka
Makara Journal of Science Vol. 8, No. 1
Publisher : UI Scholars Hub

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Abstract

Excessive diazinon residue in vegetables can endanger human health. Therefore, a simple, fast, and accurate method is needed to detect residue. A conductometric biosensor is a good choice because it also offers high selectivity and sensitivity. The principle of detection of the conductometric biosensor is based on enzymatic hydrolysis of diazinon into O,O diethyl phosphorothiate,2-isopropyl-6-methylpyrimidin-4-ol, and H+ catalyzed by organophosphate hydrolase (OPH). The optimum amount of organophosphate hydrolase added to the screen-printed carbon electrode (SPCE) modified with BSA-glutaraldehyde is 118.5 µg, while the optimum pH is 8.5. This biosensor has a response time of 30 sec, a linear dynamic range of 0 to 1 ppm, sensitivity of 42.21 µS/ppm, and limit of detection of 0.19 ppm.
Construction and Characterization of Conductometric Biosensor for Determination of the Diazinon Concentration Prayoga, Indrajid; Mulyasuryani, Ani; Prasetyawan, Sasangka
Makara Journal of Science Vol. 18, No. 1
Publisher : UI Scholars Hub

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Abstract

Excessive diazinon residue in vegetables can endanger human health. Therefore, a simple, fast, and accurate method is needed to detect residue. A conductometric biosensor is a good choice because it also offers high selectivity and sensitivity. The principle of detection of the conductometric biosensor is based on enzymatic hydrolysis of diazinon into O,O diethyl phosphorothiate,2-isopropyl-6-methylpyrimidin-4-ol, and H+ catalyzed by organophosphate hydrolase (OPH). The optimum amount of organophosphate hydrolase added to the screen-printed carbon electrode (SPCE) modified with BSA-glutaraldehyde is 118.5 µg, while the optimum pH is 8.5. This biosensor has a response time of 30 sec, a linear dynamic range of 0 to 1 ppm, sensitivity of 42.21 µS/ppm, and limit of detection of 0.19 ppm.
Co-Authors Albab, Adib Ulil Anas, M Khoirul Ani Mulyasuryani Djoko Sulistyono, Djoko Edi Santoso Eryanto, Dimas I Made Kastiawan, I Made Ichlas Wahid, Ichlas Ida Ayu Putu Sri Widnyani Ilhamsyah, Garuda Cakra Indah Nurpriyanti Mastuki Mastuki Maula Nafi Mufti, Mohammad Ninik Martini Razaq, Nuradi Mulya Sasangka Prasetyawan Setiawan, Anugrah Dwi Sulistyorini, Elisa