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All Journal Jurnal Surya Medika Martabe : Jurnal Pengabdian Kepada Masyarakat PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Bali Medika Jurnal Borneo Journal of Medical Laboratory Technology Journal of Community Development Majalah Cendekia Mengabdi
Hidayani, Al
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences

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Journal : Borneo Journal of Medical Laboratory Technology

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Imunogenesitas Protein Pilli Salmonella Typhi Pada Mencit Balb/C Dengan Mengukur Kadar TNF-α: Imunogenesity Of Protein Pilli Salmonella typhi in BALB/c Mice by Measuring TNF-α Controls Hidayani, Al; Darmawati, Sri
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6074

Abstract

Typhoid fever, often called typhoid disease, is caused by Salmonella enterica subspecies enterica serevoar typhi (Salmonella typhi). The virulence of S.typhi bacteria depends on its ability to invade host cells consisting of several components such as cell wall antigens, flagellum antigens, Vi antigens and pilli antigens. Pilli is one of the virulence factors of S.typhi bacteria, which functions as a tool for adhesion or interaction between bacteria. The study was conducted to determine how the immunogenicity of pilli protein of S.typhi bacteria isolate BA07.4 was immunized in Balb/C mice at varying doses (2-6μg) by measuring TNF- α. The analysis carried out obtained the optimum results of TNF-α levels at a dose of 3μg with an average level of 62.152 pg / ml. In the research conducted, it was found that S.typhi pilli protein can induce specific antibodies against pilli antigens. This shows that the pilli protein is immunogenic, can cause an immune response and can induce antibodies that can recognize the immunized pilli protein sub-unit.
Lama Pemanasan Carbol Fuchsin Pada Pewarnaan Preparat BTA: Heating Time Carbol Fucshin In BTA Staining Preparations Maulida, Riska; Sartika, Fera; Hidayani, Al
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.7099

Abstract

Tuberkulosis (TB paru) adalah penyakit infeksi menular yang menyerang paru-paru di sebabkan oleh bakteri Mycrobacterium tubercolusis. Salah satu pemeriksaan laboratorium yang digunakan untuk diagnosa Tubercolusis yaitu metode mikroskopis dengan pewarwaan Ziehl neelsen komponen dalam pewarnaan ini seperti Carbol Fuchsin membutuhkan adanya proses pemanasan dimana pemanasan itu akan menyerap zat warna dan akan tahan diikat tanpa mampu dilunturkan oleh peluntur yang kuat sekalipun seperti asam alkohol, tujuan penelitian ini untuk mengetahui lama pemanasan Carbol Fuchsin pada pewarnaan preparat dengan variasi waktu lama pemanasan yaitu 0 detik, 10 detik, 45 detik dan 120 detik. Sampel yang digunakan adalah sputum BTA positif yang berjumlah 6 sampel. Hasil penelitian menunjukan waktu pemanasan larutan Carbol fuchsin yang baik adalah direntang waktu 10-45 detik dimana hasil yang didapatkan warna BTA terlihat jelas, leukosit dan BTTA terlihat jelas dibandingkan dengan waktu 0 detik dan 120 detik terlihat jelas dan ada bercak merah dapat menggangu pemeriksaan.
Efektivitas Daya Hambat Ekstrak Lidah Buaya (Aloe vera) Terhadap Pertumbuhan Bakteri Staphylococcus aureus Dengan Metode Difusi: The Effectiveness Of Aloe Vera Extract On The Growth Of Staphylococcus aureus Bacteria Using Diffusion Method Hidayani, Al; Sukmawati, Vina Anggraini; Rahmah, Windya Nazmatur
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 1 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i1.7670

Abstract

Staphylococcus aureus is one of the gram-positive bacteria that causes infection in the world. Extracts from the aloe vera plant contain several ingredients, one of which is saponins, anthraquinones, and tannins. This type of research is an experiment with a laboratory observation approach because the researcher only wants to know the effectiveness of the inhibition of aloe vera extract as a natural antibiotic on the growth of Staphylococcus aureus bacteria by diffusion method. Researchers used concentrations of 75%, 50% and 25% in the diffusion method. The results showed that the average resistance obtained at a concentration of 25% was 7 mm, a concentration of 50% and 75% was 0 mm (Negative). So based on the category according to CLSI the inhibitory ability of aloe vera (Aloe vera) extract against the growth of Staphylococcus aureus bacteria is resistant, which means that aloe vera (Aloe vera) extract at concentrations of 25%, 50% and 75% is not able to inhibit the growth of Staphylococcus aureus bacteria.
Co-Authors Achmad Imam Santoso Ardina, Rinny Astuti, Mia Fauzia Dhymas Sulistyono Putro Dwi Purbayanti ERMA WIDYA LESTARI, ERMA Fera Sartika Fitria Hariati Ramdhani Gela Setya Ayu Putri Hariati Ramdhani, Fitria Hikmayanti, Hikmayanti Maulida, Riska Noor Fadillah Rezqi Handayani Sri Darmawati Suhartati, Sri Sukmawati, Vina Anggraini Wijayanti, Nira Meirita Windya Nazmatur Rahmah