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Isolasi bakteri asam laktat asal feses bayi dan potensinya dalam menghambat pertumbuhan Escherichia coli Hasbi, Nurmi; Rosyunita; Rahim, Adelia Riezka; Ayunda, Rahmah Dara; Parwata, Wayan Sulaksmana Sandhi; Farras, Afif; Raihan, Al Fikar; Billah, Muhammad Azim
Jurnal Kedokteran Universitas Palangka Raya Vol 12 No 1 (2024): Jurnal Kedokteran Universitas Palangka Raya
Publisher : Fakultas Kedokteran, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/jkupr.v12i1.12852

Abstract

Lactic acid bacteria (LAB) play an important role in improving performance and mitigating disease because of their ability to maintain the physiological balance of the gastrointestinal tract and resistance to pathogenic bacteria. LAB is one of the bacteria found in the digestive tract. LAB must be isolated from their hosts to increase their efficiency as probiotic ingredients. This study aims to obtain local isolates of LAB a from baby feces through phenotypic identification and antibacterial test. The sample used in the research was the feces of babies aged 1-6 months that patients from Mataram University Hospital. Isolation was carried out by growing bacteria from feces using the Total Plate Count method on MRSA media plus CaCO3. Phenotypic characterization is morphology test, gram stain and biochemical tests. The results of bacterial isolation obtained 8 isolates of LAB. LAB produced a clear zone in the MRSA media, because these bacteria were able to secrete acid in the MRSA. The results of gram staining show that all isolates are gram positive bacteria. Based on the morphology of LAB had characteristic 6 isolates of cocci and 2 isolates of bacilli. All isolates was able to produce an inhibition zone against E. coli with 3 isolates in the moderate category and 5 isolates in the weak category. The best inhibition zone in the medium category is for cocci-shaped bacteria.The existence of LAB isolates from baby feces can be used as a reference source for further research on probiotics such as other antibacterial test
Antibacterial Assay of Lactic Acid Bacteriea from Infant Feces on Staphylococcus aureus ATCC 25923 Raihan, Al Fikar; Rahim, Adelia Riezka; Hasbi, Nurmi
PROMOTOR Vol. 8 No. 3 (2025): JUNI
Publisher : Universitas Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/pro.v8i3.1184

Abstract

The prevalence of antibiotic resistance is a serious global health problem and a threat to human health. Staphylococcus aureus is a Gram-positive bacterium that can cause various infections in humans, such as skin infections, sepsis, endocarditis, osteomyelitis, and toxins that cause food poisoning. One way to overcome the problem of antibiotic resistance is to use Lactic Acid Bacteria (LAB). LAB has many benefits, including being able to function in food fermentation, produce antimicrobial compounds, and act as probiotics. This study aims to determine the inhibitory effect of LAB from baby feces as a natural antibacterial agent against S. aureus. The method used in this study was the agar well diffusion method. The number of LAB isolates used was 8 consisting of seven cocci and two bacilli forms and also used 2 controls, namely, negative control NaCl 0.9% and Positive Control Ciprofloxacin. This study consisted of six stages including media preparation, preparation of test bacteria, sample preparation, making suspensions of S. aureus and LAB, making Mcfarland solution, and antibacterial testing using the agar well diffusion method for 3 repetitions of the treatment. The results obtained showed that all LAB isolates were able to produce an inhibition zone against Staphylococcus aureus bacteria with the results of the isolates in diameter 01A 10-5 (7.67mm), 01A 10-6(2) (6mm), 03A10-7(1) (6.67mm), 01F 10-6(2) (2mm), 01F10-7(2) (1.67mm), 02AF10-7(1) (4.33mm), 03AF10-7(2) (4mm), 04AF10-7 (3.67mm). Based on the results of the Mann-Whitney test, it was found that all LAB isolates had significant differences from the positive control Ciprofloxacin. So it can be seen that LAB from baby feces is less effective in replacing antibiotics.
Deep Vein Thrombosis (DVT): A Literature Review Kamila, Alya Syafa; Ginuluh, Gintis Dhimar; Syakir, Syahda Aqila; Abida, Aisya Nur; Balqis, Ananda Amirah; Karlina, Fairuz; Murniatin, Rinesita; Farras, Afif; Raihan, Al Fikar; Putra, Rian Akka Jaya; Arsyatt, Maz Isa Ansyori
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8008

Abstract

Deep Vein Thromboembolism (DVT) occurs due to the formation of blood clots in the veins, characterized by the formation of thrombi along with an inflammatory response in the inner walls of the veins. DVT most commonly occurs in the deep veins of the lower extremities but can also occur, albeit less frequently, in other areas such as the arms or other forms of superficial thrombosis. This article aims to review the epidemiology, clinical presentation, diagnosis, pathophysiology, and management of deep vein thrombosis. The literature review was conducted by searching for relevant articles on deep vein thrombosis published in the past fifteen years. Literature searches were performed using Google Scholar, PubMed, and NCBI databases. The findings highlight the clinical manifestations of DVT and its potential to cause serious complications, particularly post-thrombotic syndrome. Post-thrombotic syndrome is a debilitating condition that can significantly impair patients' quality of life. Management of DVT involves both pharmacological and non-pharmacological treatments. Understanding the mechanisms of deep vein thrombosis and its management is crucial in minimizing the complications associated with this condition.