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Anticancer Activity Of Apis Dorsata Binghami Nest Extract From West Uluway, Tana Toraja District Patandean, Selvrining; Mokosuli, Yermia Semuel; Ogi, Nova LIM; Djarang, Rievo H; Manampiring, Nonny; Moko, Emma Mauren
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12750

Cancer is the leading cause of death in the world, with a high prevalence in Indonesia, especially breast cancer. Conventional therapies such as chemotherapy and radiotherapy have various limitations, including severe side effects and cancer cell resistance. This study aims to explore the anticancer potential of the ethanol extract of Apis dorsata Binghami nests originating from Uluway Barat Village, Tana Toraja Regency. Extraction was carried out using the maceration method with 96% ethanol solvent, with a yield of 51.42%, and bioactive compounds were identified through GC-MS analysis. Anticancer activity tests were carried out in vitro on MCF-7 breast cancer cells using the resazurin assay method . The results showed that the extract contained various bioactive compounds such as flavonoids, furanones, and aromatic aldehydes which are known to have antioxidant and anticancer activities. The IC50 value obtained was 434.30 µg/mL, indicating weak cytotoxic activity. Nevertheless, this extract showed significant ability to kill cancer cells at certain concentrations, especially 15.63 µg/mL which reduced cell viability by 44.39%. These results indicate that A. dorsata Binghami nests contain compounds that have the potential as natural anticancer agents, but require further research for fractionation and identification of more specific active compounds and further testing in vivo

EFFECT OF GEL FORMULATION CONTAINING Tridax procumbens AND Aloe vera LEAF EXTRACT ON THE HEALING OF SECOND-DEGREE BURNS IN MICE (Mus musculus) Maturbongs, Marsela; Setyawati, Iriani; Manampiring, Nonny; Lawalata, Helen; Taulu, Marthy; Rompas, Christny
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/n2vkg912

Tridax procumbens L. and Aloe vera L. are plants commonly used in the treatment of burns. This study was conducted to determine the effect of giving a combination gel of T. procumbens and A. vera leaf extract on second-degree burn healing in mice. This study used 24 male mice (Mus musculus) which were divided into four treatment groups, namely positive control (KP, Bioplacenton®); negative control (KN, gel base); leaf extract of T. procumbens 0.5% and A. vera 0.5% (P1); and leaf extract of T. procumbens 1% and A. vera 0.5% (P2). The process of inducing burns on the backs of mice requires the use of a 1x1 cm iron, which is subjected to heat in a fire for three minutes. The iron is placed on the mice's back for five seconds, which leads to the development of a second-degree burn. The gel was applied twice a day for 21 days. The highest level of burn wound healing was recorded with the Bioplacenton® treatment, which achieved a full recovery of 100% on day 15. Following this, the combined gel treatment of 1% T. procumbens leaf extract and 0.5% A. vera also reached 100% healing by day 18. While it may not be as potent as Bioplacenton®, the gel formulation containing 1% T. procumbens and 0.5% A. vera leaf extract presents a viable alternative for treating second-degree burns.

EFFECT OF GEL FORMULATION CONTAINING Tridax procumbens AND Aloe vera LEAF EXTRACT ON THE HEALING OF SECOND-DEGREE BURNS IN MICE (Mus musculus) Maturbongs, Marsela; Setyawati, Iriani; Manampiring, Nonny; Lawalata, Helen; Taulu, Marthy; Rompas, Christny
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/n2vkg912

Tridax procumbens L. and Aloe vera L. are plants commonly used in the treatment of burns. This study was conducted to determine the effect of giving a combination gel of T. procumbens and A. vera leaf extract on second-degree burn healing in mice. This study used 24 male mice (Mus musculus) which were divided into four treatment groups, namely positive control (KP, Bioplacenton®); negative control (KN, gel base); leaf extract of T. procumbens 0.5% and A. vera 0.5% (P1); and leaf extract of T. procumbens 1% and A. vera 0.5% (P2). The process of inducing burns on the backs of mice requires the use of a 1x1 cm iron, which is subjected to heat in a fire for three minutes. The iron is placed on the mice's back for five seconds, which leads to the development of a second-degree burn. The gel was applied twice a day for 21 days. The highest level of burn wound healing was recorded with the Bioplacenton® treatment, which achieved a full recovery of 100% on day 15. Following this, the combined gel treatment of 1% T. procumbens leaf extract and 0.5% A. vera also reached 100% healing by day 18. While it may not be as potent as Bioplacenton®, the gel formulation containing 1% T. procumbens and 0.5% A. vera leaf extract presents a viable alternative for treating second-degree burns.

Pengembangan Modul Berbasis Riset Keanekaragaman Spesies Ikan di Danau Tondano terhadap Literasi Sains Mahasiswa Pendidikan Biologi Manampiring, Nonny; Mamahit, Jimmi Andrew; Posumah, Dany Ch.
SCIENING : Science Learning Journal Vol. 4 No. 1: Juni 2023
Publisher : Jurusan Pendidikan IPA FMIPAK Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/slj.v4i1.6629

Penelitian ini bertujuan untuk mengembangkan produk berupa modul pembelajaran berbasis hasil riset keanekaragaman spesies ikan di danau Tondano pada mata kuliah Biodiversitas Sulawesi Penelitian ini menggunakan jenis penelitian dengan desain pengembangan ADDIE analyze design development implementation evaluation Pengembangan modul ajar ini dilakukan dalam menjawab permasalahan terhadap kendala mahasiswa dalam memperoleh sumber referensi belajar yang lebih spesifik dikarenakan pada mata kuliah tersebut belum melakukan pengembangan tentang biodiversitas fauna khususnya keanekaragaman ikan di daerah danau Tondano Modul yang dikembangkan melaui penyusunan storyboard terlebih dahulu untuk memudahkan menyusun produk akhir Modul hasil pengembangan terlebih dahulu divalidasi oleh ahli materi dengan hasil validasi sebesar 97 48 dengan kategori sangat valid dan validasi ahli media dengan hasil validasi sebesar 93 90 dengan kategori sangat valid Uji efektivitas modul terhadap literasi sains dengan perolehan nilai N Gain 87 70 dengan kategori sangat efektif dan pembagian penskoran memperoleh nilai N Gain 0 88 dengan kategori tinggi Hasil respon mahasiswa terhadap modul menunjukkan nilai sebesar 95 61 dengan kategori sangat praktis < p>

Effectiveness of Sea Cucumber (Holothuria atra) Extract Ointment from the Talaud Islands on Burn Wound Healing in Mice (Mus musculus) Mumu, Trivena Vannesa; Roring, Verawati Ida Yani; Manampiring, Nonny
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p061-070

Sea cucumber (Holothuria atra) has been traditionally used for wound treatment; however, scientific evidence supporting its effectiveness in burn wound healing remains limited. This study presents a novel investigation of the wound healing potential of H. atra extract sourced specifically from the Talaud Islands and formulated into multiple topical ointment concentrations for second-degree burn treatment. The study aimed to evaluate the effectiveness of H. atra extract ointment in accelerating second degree burn wound healing in mice. The extract was obtained by maceration using 95% ethanol and formulated into ointments at concentrations of 10%, 15%, and 20%. Twenty-five male mice were randomly assigned to five groups: positive control (1% silver sulfadiazine), negative control, and three treatment groups receiving H. atra extract ointments at different concentrations. Parameters observed included wound diameter reduction, physical characteristics of the ointment, phytochemical screening, and pain-related behavioral responses. The results demonstrated that the 15% H. atra extract ointment produced the highest wound healing effectiveness, with a wound contraction rate of 96% on day 14, followed by the 10% (84%) and 20% (75%) formulations. Phytochemical analysis revealed the presence of flavonoids, saponins, steroids, and alkaloids, which are associated with anti-inflammatory, antimicrobial, and tissue regenerative activities. The ointment formulations met homogeneity and organoleptic requirements, although their spreadability values were below the recommended standard. These findings highlight the novelty of identifying an optimal concentration (15%) of H. atra extract ointment that provides superior burn wound healing activity compared with other tested concentrations. In conclusion, the 15% H. atra extract ointment shows potential as an alternative topical therapy for second-degree burn wounds.

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