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All Journal Bioma : Berkala Ilmiah Biologi Jurnal Biogenerasi BIO-EDU: Jurnal Pendidikan Biologi Biofarm : Jurnal Ilmiah Pertanian Kenanga : Journal of Biological sciences and Applied Biology
Lestari, Septi Wahyu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Other

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Journal : Bioma : Berkala Ilmiah Biologi

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Supporting Environmental Resilience through Biological Approach: Initial Step for Building Robusta Coffee Germplasm Database Rustiati, Elly Lestari; Priyambodo, Priyambodo; Pratiwi, Dian Neli; Srihanto, Eko Agus; Lestari, Septi Wahyu; Sandra, Shifa; Winarno, Winarno; Thesalonika, Natasya
Bioma : Berkala Ilmiah Biologi Vol. 26 No 2, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.65548

Abstract

In response to environmental challenges and the critical imperative for sustainable progress, rural regions including agriculture practices are important in ecological robustness. It can be achieved through a synergistic relationship between rural development sustainability and the biological sciences. Concurrently, research in stewardship approaches are underway to amass data on robusta coffee, Coffea canephora, species. In collaboration with Lampung Disease Investigation Centre and local coffee farmers in Pesawaran, Lampung, and under DIPA BLU Universitas Lampung year 2024, initial ongoing work on coffee germ plasm data is done. Robusta coffee leaves were sampled from 2 blocks of traditional coffee plantations, Wiyono and Bogorejo, Pesawaran, Lampung.  Of 18 robusta leaves’ samples DNA extraction, electrophoresis showed 14 samples with good results. The optimum temperature for amplification is 62 °C and 63 °C.  The results will be sequenced for its species confirmation.  This project is expected to contribute bottom up towards inclusive and sustainable rural futures at the local level.
Co-Authors Adelia, Ersa Imelda Afandi, Aril Alfandi, Aril Ashari, Minanti Mayda Azzahra, Laila Salwa Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Febriansyah, Muhammad Gina Dania Pratami, Gina Dania Maharani, Annisa Lidya Permatasari, Nindy Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Raden Mohamad Herdian Bhakti Ramadan, Viki Rustiaati, Elly Lestari Sandra, Shifa Suhada Suhada, Suhada Thesalonika, Natasya WINARNO Winarno Winarno Yuliana Andriyani