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All Journal Jurnal Pharmacia Mandala Waluya Jurnal MediLab Mandala Waluya
Syawal Abdurrahman
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Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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DETEKSI GEN TEMONEIRA (TEM) PADA URIN PENDERITA GAGALGINJAL KRONIK (GGK) SEBAGAI PENANDA RESISTENSI ANTIBIOTIK DENGANMENGGUNAKAN METODE POLYMERASE CHAIN REACTION (PCR) DI RSUD BAHTERAMAS PROVINSI SULAWESI TENGGARA Syawal Abdurrahman; Sanatang; Shalsa Juniastrid Masiar
Jurnal MediLab Mandala Waluya Vol. 9 No. 2 (2025): JURNAL MEDILAB MANDALA WALUYA
Publisher : Prodi D4 Teknologi Laboratorium Medis, Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54883/medilab.v9i2.1420

Abstract

Chronic kidney disease (CKD) is a condition in which the kidneys experience a decline in kidney function. Chronic kidney disease (CKD) causes a decline in immunity, metabolism, and catheter use, which can increase the risk of urinary tract infections (UTIs). Urinary tract infections (UTIs) are common infections caused by the pathogenic bacterium Escherichia coli. The Temoneira (TEM) gene is one of the genes specifically associated with resistance to β-lactamase antibiotics. The objective of this study is to detect the TEM gene as a marker of antibiotic resistance in the urine of patients with chronic kidney disease using the Polymerase Chain Reaction (PCR) method. The type of research conducted was quantitative research. The population in this study consisted of 12 respondents with chronic kidney disease (CKD) at the Bahteramas Regional General Hospital in Southeast Sulawesi Province, while the sample size was 10 respondents, representing the entire population. The research methods included DNA isolation, DNA concentration measurement, TEM gene amplification, and visualization of the results using agarose gel electrophoresis. Based on the absorbance measurements of the DNA isolates, a DNA purity ratio below 1.8 was obtained, indicating protein contamination. DNA visualization results showed that there were 10 samples that exhibited an 861 bp DNA band, indicating the presence of the TEM gene as an antibiotic resistance marker. The conclusion of this study is that out of 10 respondents, there were 10 positive samples of the TEM gene, marked by the appearance of an 861 bp DNA band. Further research is recommended to continue using the DNA sequencing method to confirm the variation of the TEM gene mutation..
Penentuan Kadar Flavonoid Total Dan Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Batang Gayam (Inocarpus fagiferus Fosb.) Dengan Metode CUPRAC (Cupric Ion Reducing Antioxidant Capacity) Ayu Anastarisa Ayu; Syawal Abdurrahman; Firhani Anggriani Syafrie; Putri Mega Wijayanti; Fitriani W. Alani; Wa Ode Ida Fitriah
Jurnal Pharmacia Mandala Waluya Vol. 5 No. 3 (2026): Jurnal Pharmacia Mandala Waluya
Publisher : Prodi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54883/jpmw.v5i3.649

Abstract

Indonesia memiliki kekayaan hayati yang melimpah, termasuk tanaman gayam (Inocarpus fagiferus Fosb.) yang diketahui mengandung senyawa flavonoid dengan aktivitas antioksidan. Flavonoid berperan penting dalam menangkal radikal bebas yang dapat memicu berbagai penyakit degeneratif. Penelitian ini bertujuan untuk menentukan kadar flavonoid total dan menguji aktivitas antioksidan ekstrak etanol kulit batang gayam menggunakan metode Cupric Ion Reducing Antioxidant Capacity (CUPRAC). Penelitian dilakukan secara eksperimental dengan ekstraksi simplisia kulit batang gayam menggunakan metode maserasi dengan pelarut etanol 96%. Kadar flavonoid total diukur menggunakan standar kuersetin melalui spektrofotometri UV-Vis, sedangkan uji aktivitas antioksidan dilakukan dengan metode CUPRAC dan dinyatakan dalam nilai IC50. Data dianalisis dengan regresi linier untuk memperoleh hubungan antara konsentrasi dan persentase inhibisi. Hasil penelitian menunjukkan rendemen ekstrak sebesar 5,76% dengan kadar flavonoid total sebesar 6,525% (6,525 mg QE/g ekstrak). Aktivitas antioksidan ekstrak menghasilkan nilai IC50 sebesar 3,833 μg/mL yang tergolong kategori sangat kuat, sedangkan vitamin C sebagai pembanding memiliki nilai IC50 sebesar 2,957 μg/mL. Nilai R² yang tinggi menunjukkan hubungan linier yang baik antara konsentrasi ekstrak dan persentase inhibisi. Sehingga dapat disimpulkan bahwa ekstrak etanol kulit batang gayam memiliki kadar flavonoid yang cukup tinggi dan aktivitas antioksidan yang sangat kuat, sehingga berpotensi dikembangkan sebagai sumber antioksidan alami untuk aplikasi kesehatan dan farmasi.
Co-Authors Ayu Anastarisa Ayu Dinda Rizki Amalia Firhani Anggriani Syafrie Fitriani W. Alani Ishma Marsidin Mien Asrha Suhardin Mus Ifaya Putri Mega Wijayanti Rasyid, Sri Anggarini Safitriyani Samsudin Sanatang Shalsa Juniastrid Masiar Tasman Ulis Safitri Wa Ode Ida Fitriah Wa Ode Nur Intan Yayu Sri Rahayu Yunita Amraeni Yusuf Useng