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All Journal Jurnal Labora Medika Jurnal Biomedika Jurnal Medikes (Media Informasi Kesehatan) Health Information : Jurnal Penelitian PUINOVAKESMAS Jurnal Info Kesehatan Jurnal Teknokes
Zulfikar Husni Faruq
Poltekkes Kemenkes Yogyakarta
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Isolation, Quantification, and Plaque Morphology Analysis of Lytic Bacteriophages from River Water Targeting Clinical MDR Klebsiella pneumoniae Using the Double-Layer Agar Method Aminah, Aminah; Trisna, Citra; Sugianto, Fitri Alina; Amalia, Gadis; Martiningsih, M Atik; Faruq, Zulfikar Husni
Jurnal Teknokes Vol. 18 No. 4 (2025): Desember
Publisher : Jurusan Teknik Elektromedik, Politeknik Kesehatan Kemenkes Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/jteknokes.v18i4.113

Abstract

Antimicrobial resistance is a growing global health threat and is projected to cause up to 10 million deaths per year by 2050. Klebsiella pneumoniae is a priority pathogen due to its multidrug resistance (MDR) mechanisms, such as extended-spectrum β-lactamases and carbapenemases, which significantly limit therapeutic options and increase the need for antimicrobial alternatives. This study aimed to isolate and quantify active lytic bacteriophages capable of infecting clinical MDR K. pneumoniae from river water samples. Water samples were processed by centrifugation and membrane filtration to remove debris and bacterial cells, then incubated with MDR K. pneumoniae in Luria broth at 37°C to enhance phage adsorption and amplification. Phage detection and enumeration were performed using the double-layer agar method. Plaque morphology was observed to confirm lytic activity, while serial dilutions were used to determine phage titer. Several lytic bacteriophages were successfully isolated from river water samples. The plaques formed were clear, spherical, and well-defined, with some exhibiting halos indicative of possible depolymerase activity. Phage titers ranged from 1.28 × 10³ to 2.00 × 10⁶ PFU/mL, indicating efficient replication against MDR K. pneumoniae without repeated enrichment processes. River water is a potential source of lytic bacteriophages capable of infecting MDR K. pneumoniae. These findings emphasize the role of aquatic environments as natural reservoirs of phages with potential use in the development of future antimicrobial or biocontrol strategies and support the need for further studies on the host range, stability, and therapeutic applications of the isolated phages.
Co-Authors Amalia, Gadis Aminah Aminah Atik Ismiyati Budi Setiawan Fitri Nuroini Heppinsky Puspita Kinasih Kasiyati, Menik M Atik Martiningsih, M Atik Martsiningsih, Marta Atik Muji Rahayu Naili Hilda Atifa Pudyastuti, Rita Rena Rahmad Rahmawati, Ully Sistiyono Sistiyono, Sistiyono Siti Nuryani, Siti Sugianto, Fitri Alina Suyana Suyana, Suyana Trisna, Citra Ullya Rahmawati