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Studi In Silico Potensi Ekstrak Lipida Tetraselmis chuii Sebagai Antioksidan Bintari, Yoni Rina
JU-ke: Jurnal Ketahanan Pangan Vol 2, No 1 (2018): JU-Ke
Publisher : Universitas Islam Malang

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Tetraselmis chuii merupakan mikroalga laut yang potensial sebagai sumber antioksidan. Dari penelitian sebelumnya T. chuii yang diekstrak dengan metode Micowave Assisted Extraction (MAE) didapatkan senyawa aktif linoleic acid, linolenic acid, oleic acid, palmitic acid, palmitoleic acid, stearic acid, dan tetradecanoic acid. Studi pendahuluan potensi senyawa aktif ekstrak T.chuii dengan metode in silico menggunakan bantuan software pyrex autodock vina, dengan bantuan visualisasi menggunakan biovia discovery studio dan pymol. Hasil yang diperoleh dari studi in silico menunjukkan bahwa ekstrak T. chuii potensial sebagai senyawa antioksidan. Hal ini ditunjukkan nilai binding affinity dengan protein reseptor SOD, memiliki nilai lebih negative dibandingkan dengan kontrolnya.Â Interaksi ekstrak T. chuii cukup potensial sebagai antioksidan, melalui ikatan hidrogen dengan residu asam amino Val 8 dan Val 149.Â Kata kunci: Tetraselmis chuii, in silico, SOD, antioksidan, lipid

Ekstraksi Lipida Dengan Metode Microwave Assisted Extraction Dari Mikroalga Yang Potensial sebagai Biodiesel Bintari, Yoni Rina; Haryadi, Winarto; Rahardjo, Tri Joko
JU-ke: Jurnal Ketahanan Pangan Vol 2, No 2 (2018): JU-Ke
Publisher : Universitas Islam Malang

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Important stages of the production of biodiesel from microalgae is a lipid extraction step. Microwave Assisted Extraction (MAE) is an extraction technique utilizing microwave energy using water solvent. Excellence MAE method is to minimize the use of organic solvents that are toxic, no ecofriendly and unrenewable. Extraction of lipids from microalgae using MAE method leads to a green process to produce bioenergy. This study aims to obtain the optimum extraction conditions of S. platensis lipids and T. chuii using MAE method, the effect of the addition of n-hexane at a post extraction and conversion to biodiesel.Â The results showed that the optimum of lipid extraction conditions from S. platensisÂ at microwave power of 560 Watts, the addition of water to the volume of microalgae 6.67 mL/gram dry weight of S. platensis and extraction time of 510 seconds resulting lipid yield of 1.27%. The optimum of lipid extraction condition from T. chuii at 800 Watts of power, the addition of water to the volume of microalgae 8.3 mL/gram dry weight T. chuii and extraction time for 510 seconds resulting lipid yield of 1.25%. Increasing the number of n-hexane at a post extraction from S. platensis effective in adding volume 5 mL, where the addition of 7 mL to 15 mL have no significant increase in yield. Conversion of S. platensis lipids and T. chuii produced a high yield of biodiesel in comparison to the amount of lipid methanol 1:173 to yield 66,7% and 75% respectively.

Ekstraksi Senyawa Bioaktif dari Cladophora sp. Dengan Metode Solvent Free Microwave Assisted Extraction (SFMAE) Yoni Rina Bintari; Helmin Elyani
JIMR - Journal of Islamic Medicine Research Vol 1, No 1 (2017)
Publisher : Published by Faculty of Medicine Universitas Islam Malang

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Introduction: Extraction stages is important, in order to discovery of bioactive compounds that have pharmalogical effects. Solvent Free Microwave Assisted Extraction (SFMAE) is alternative procedure with criteria of green chemistry and sustainability. Methods: In order the examine the effect of microwave power, six power level 0%, 20%, 40%, 60%, 80%, and 100% (0 W, 200 W, 400 W, 600 W, 800 W and 1000 W) were studied. The variation of adding water 10 ml/g, 15 ml/g, 20 ml/g and 30 ml/g were studied. Optimum condition of extraction from the highest yield. Phytochemichal screening using specific reagent to know bioactive compounds group.Results: The result obtained is the highest yield of 3,7333% with 400 W microwave power and 30 ml/g adding water in 360 seconds. The results of phytochemical screening of Cladophora Sp extract contain secondary metabolites of alkaloid, phenolic, terpenoid and saponin groups.Conclusion: From phytochemical screening, Cladophora sp. has potential of antioxidant and antibacterial activity, so the next research focus to antioxidant and antibacterial activity.Keywords: Extraction, SFMAE, Cladophora sp., Phytochemical

In Silico Studies on Bidara (Ziziphus mauritiana) Leaves Ethanol Extract Bioactive Ligands compared to Acarbose toward ?-glucosidase Enzyme Merlita Herbani; Yoni Rina Bintari
JIMR - Journal of Islamic Medicine Research Vol 1, No 2 (2017)
Publisher : Published by Faculty of Medicine Universitas Islam Malang

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ABSTRACTIntroduction : Diabetes mellitus has been widely concerned in human health. Diabetes mellitus has a huge amount of sufferers. Carbohydrate diet, which is needed for energy supply, contributes to uncontrolled postprandial blood glucose which worsens diabetes mellitus. ?-glucosidase is an enzyme that had been targeted to modify oligosaccharides, to decrease postprandial blood glucose. This study aims to predict the ability of the active compound contained in Z. mauritiana leaves to inhibit ?-glucosidase protein through an in silico study.Methods : Identified local Z. mauritiana leaves simplicial were extracted with 96% p.a ethanol solvent. The extract then analyzed with LCMS to confirm 6 active compound in the extract. Confirmed active compounds then undergo docking procedure, by downloading active compounds and acarbose molecular structure via PubChem, then converted to be 3D structure in pdb format by Pymol. Protein target ?-glucosidase molecular structure were obtained from Uniprot, and 3D structure built by Swissmodel. Docking process held by Pyrex by AutoDock Vina. Protein interaction ligands were visualized by Biovia Discovery Studio.Results : LCMS analysis revealed that there are apigenin, quercetin, routine, kaempferol, isovitexin and quercetin-3-rhamnoside in the extract. Molecular docking analysis explained that rutin has higher binding affinity than acarbose as the control. Rutin shared the same hydrogen bonding with acarbose in Arg 275 and Val 544.Conclusion : In conclusion, Z. mauritiana have potential as ?-glucosidase inhibitor.KEYWORDS : Ziziphus mauritiana ethanol extract Active Compound, Acarbose, in silico, ?-glucosidase

The growth rate of S. aureus when treated with Azithromycin alone and combined with Syzygium cumini decoctation Rio Risandiansyah; Yoni Rina Bintari
JIMR - Journal of Islamic Medicine Research Vol 1, No 2 (2017)
Publisher : Published by Faculty of Medicine Universitas Islam Malang

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ABSTRACTIntroductiom: Resistance to antibiotics is the result of adaptive and spontaneous mutations, which can be suppressed by use of combinatory antibiotics. Synergistic effects between certain plants in combination with antibiotics is known, yet the mutation frequency of such combinations is still unexplored. Therefore, this study aims to measure the spontaneous mutational frequency of S. aureus for Azithromycin (AZM) resistance alone and in combination with Syzygium cumini decocta (SCD), known for its antimutagenic effect. Method: This study uses fluctuation analysis with 39 replicate cultures in selective media using AZM alone, SCD alone, and AZM combined with SCD at 1x MIC and analysing resistant strains based on its log phase delay in liquid media in a 24-hour period. AZM and SCD showed antibiotic activity against S. aureus. Results: Time-point growth comparison showed two cultures in AZM selective medium with no delayed log phase between S. aureus in non-selective medium and absorbance >1.0 after 24 hours, indicating resistance to AZM. Conclusion: Increased growth suppression was observed in combination between AZM combined with SCD, compared to AZM and SCD alone. The spontaneous mutation frequency of S. aureus against AZM was 0.182, while both SCD and AZM combined with SCD had zero mutational events for antibiotic resistance.Keywords:Antibiotic Resistance, Combinatorial Antibiotic Therapy (CAT), Mutation frequency, Syzygium cumini.

In Silico Studies on Bidara (Ziziphus mauritiana) Leaves Ethanol Extract Bioactive Ligands compared to Acarbose toward ?-glucosidase Enzyme Merlita Herbani; yoni Rina Bintari
JIMR - Journal of Islamic Medicine Research Vol 1, No 2 (2017)
Publisher : Published by Faculty of Medicine Universitas Islam Malang

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ABSTRACTIntroduction : Diabetes mellitus has been widely concerned in human health. Diabetes mellitus has a huge amount of sufferers. Carbohydrate diet, which is needed for energy supply, contributes to uncontrolled postprandial blood glucose which worsens diabetes mellitus. ?-glucosidase is an enzyme that had been targeted to modify oligosaccharides, to decrease postprandial blood glucose. This study aims to predict the ability of the active compound contained in Z. mauritiana leaves to inhibit ?-glucosidase protein through an in silico study.Methods : Identified local Z. mauritiana leaves simplicial were extracted with 96% p.a ethanol solvent. The extract then analyzed with LCMS to confirm 6 active compound in the extract. Confirmed active compounds then undergo docking procedure, by downloading active compounds and acarbose molecular structure via PubChem, then converted to be 3D structure in pdb format by Pymol. Protein target ?-glucosidase molecular structure were obtained from Uniprot, and 3D structure built by Swissmodel. Docking process held by Pyrex by AutoDock Vina. Protein interaction ligands were visualized by Biovia Discovery Studio.Results : LCMS analysis revealed that there are apigenin, quercetin, routine, kaempferol, isovitexin and quercetin-3-rhamnoside in the extract. Molecular docking analysis explained that rutin has higher binding affinity than acarbose as the control. Rutin shared the same hydrogen bonding with acarbose in Arg 275 and Val 544.Conclusion : In conclusion, Z. mauritiana have potential as ?-glucosidase inhibitor.KEYWORDS : Ziziphus mauritiana ethanol extract Active Compound, Acarbose, in silico, ?-glucosidase

Studi Insilico Potensi Minyak Astiri dan Ekstrak Etanol Daun Annona muricata Sebagai Calon Herbal Terstandart Untuk Analgesik dan Antiinflamasi Reza Hakim; Yoni Rina Bintari; Dini Sri Damayanti
Jurnal Kesehatan Islam : Islamic Health Journal Vol 7, No 1 (2018): Jurnal Kesehatan Islam : Islamic Health Journal
Publisher : Publikasi oleh Fakultas Kedokteran Universitas Islam Malang

ABSTRACTInflammatory reactions give rise to a series of processes and symptoms that can cause pain and damage to nearby cells. Inflammatory reactions are mediated by inflammatory and proinflammatory cytokines. NSAID class (non-steroidal anti-inflammatory drug) drugs have been developed to inhibit the inflammatory process by suppressing COX-1 and / or COX-2 enzyme activity. However, this drug can cause gastric mucosal irritation. the ethanol extract of Annona muricata leaf has an analgesic and anti-inflammatory effect[3].The purpose of this research is to know analgesic and antiinflamatory activity from ethanol extract and essential oil of Annona muricata leaf.The method used was the study of in silico by molecular docking of active compounds in ethanol extracts and essential oils to the COX-1, COX-2, phospolipase-A, and TNF-alpha receptor molecules as target molecules. The molecular docking results show that Annona muricata ethanol extract can act as an anti-inflammatory and analgesic, especially through strong bonds between "rutine" compounds with COX-2 receptors with affinity -10 kcal / mold and "quercetin" with affinity bindings -9.8 kkal.mol, better than dexametason (binding affinity -9.6 kcal / mol). Keywords:Inflamation, COX-1, COX-2, phospolipase-A, TNF-alfa, docking

PENGARUH VARIASI KONSENTRASI SUKROSA TERHADAP DERAJAT KEASAMAN DAN TOTAL BAKTERI ASAM LAKTAT KOMBUCHA DAUN SIRSAK (Annona muricata L.) Rosita Sari; Yoni Rina Bintari; Dini Sri Damayanti
Jurnal Kedokteran Komunitas Vol 10, No 1 (2022)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

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ABSTRAKPendahuluan: Kombucha daun sirsak merupakan minuman probiotik yang berasal dari hasil fermentasi teh daun sirsak dan sukrosa oleh starter kombucha yaitu SCOBY (Symbiotic Culture of Bacteria and Yeast). Daun sirsak dapat digunakan sebagai bahan dasar untuk pembuatan kombucha karena mengandung senyawa polifenol yang tinggi. Salah satu bakteri pada kombucha yang mempunyai peran sebagai probiotik adalah bakteri asam laktat, sedangkan yang mempengaruhi pertumbuhan dari bakteri asam laktat adalah sukrosa. Penelitian ini bertujuan untuk mengukur total bakteri asam laktat dan derajat keasaman pada kombucha daun sirsak dengan variasi konsentrasi sukrosa berbeda.Metode: Kombucha daun sirsak dibuat dengan menambahkan sukrosa dengan konsentrasi 5%, 10%, 15% (b/v) serta SCOBY dan difermentasi selama 7 hari. Pengukuran derajat keasaman menggunakan pH meter yang telah dikalibrasi. Penghitungan total bakteri asam laktat dinyatakan dalam Colony Forming Unit (CFU)/ml. Hasil dianalisis secara statistik menggunakan uji one way ANOVA dengan taraf signifikansi p≤0.05.Hasil: Tidak terdapat perbedaan secara signifikan variasi kadar sukrosa terhadap derajat keasaman dan total bakteri asam laktat kombucha daun sirsak. Kadar sukrosa 10% (b/v) pada kombucha daun sirsak mempunyai derajat keasaman 3,30 ± 0,06 dan total bakteri asam laktat sebanyak 9,03 x 106 ± 5,36 x 106 CFU/ml yang sesuai untuk dikonsumsi.Kesimpulan: Kombucha daun sirsak konsentrasi gula 10% (b/v) mempunyai derajat keasaman dan total bakteri asam laktat lebih tinggi jika dibandingkan dengan kombucha daun teh sehingga lebih berpotensi sebagai probiotik.Kata Kunci: Kombucha, daun sirsak, probiotik, derajat keasaman, total bakteri asam laktat.

Piper betle L. SEBAGAI PEWARNA BAKTERI : UJI AKURASI DAN PRESISI WARNA PADA Staphylococcus aureus DAN Escherichia coli Annur Affin Niswah; Yoni Rina Bintari; Rio Risandiansyah
Jurnal Kedokteran Komunitas Vol 10, No 2 (2022)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

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ABSTRAKPendahuluan: Tanaman Piper betle L.(PB) memiliki kandungan pigmen antosianin dan tannin warna hijau kecoklatanyang dapat digunakan sebagai zat warna pada pewarnaan bakteri sederhana untuk mengurangi dampak negatifpenggunaan pewarna sintesis. Namun, tingkat efektivitas PB sebagai pewarnaan sederhana bakteri belum diketahuisehingga perlu dilakukan uji akurasi dan presisi PB sebagai pewarnaan sederhana bakteri.Metode: Penelitian eksperimental in vitro menggunakan bakteri S. aureus dan E. coli yang dilakukan pewarnaansederhana dengan ekstrak metanol PB (2,5%, 5%, 10%)v/v, methylene blue (kontrol positif) dan metanol (kontrolnegatif). Hasil pewarnaan diamati secara deskriptif kualitatif oleh tiga pengamat pada lima lapang pandang dari gambarmikroskop cahaya. Perhitungan kuantitatif dilakukan dengan imageJ yang dihitung nilai akurasi dan presisinya secaramatematis. Hasil dianalisa dengan Mann-Whitney dan signifikasi ditetapkan pada p<0.05.Hasil: Pewarna methylene blue (MB) memberikan hasil pewarnaan kualitatif 100% sedangkan metanol 0%. Pewarnaanekstrak metanol PB konsentrasi 2,5%, 5%, 10% menunjukkan hasil pengamatan deskriptif kualitatif yaitu 2,40, 5,38,3,22 pada S. aureus dan 2,77, 4,94, 2,68 pada E. coli. Hasil kuantitatif didapatkan nilai akurasi S. aureus yakni 240,51%,158,49%, 160,91% dan 81,22%, 107,02%, 63,01% pada E. coli dengan nilai presisinya yaitu 35,21%, 26,28%, 31,51%pada S. aureus dan 42,14%, 23,78%, 26,23% pada E. coli. Hasil analisa kualitatif dan kuantitatif menunjukkan bahwaekstrak metanol PB 5% memiliki kualitas lebih baik daripada 2,5% dan 10%. Adanya bias berupa genangan minyak(micelles) diperkirakan mengganggu perhitungan bakteri dengan imageJ dan kelemahan dalam pemilihan metodepenelitian.Kesimpulan: Ekstrak metanol PB mengandung pigmen zat warna. Namun, uji akurasi dan presisi menunjukkan lebihrendah daripada kontrol positif.Kata Kunci: Daun sirih, pewarnaan sederhana, S. aureus, E. coli, akurasi, presisi

EFEK KOMBINASI FRAKSI ALANG-ALANG (Imperata cylindrica, L.) DENGAN Amoxicilin ATAU Chloramphenicol TERHADAP DAYA HAMBAT Staphylococcus aureus Faris Akbar Maulana; Rio Risandiansyah; Yoni Rina Bintari
Jurnal Kedokteran Komunitas Vol 9, No 1 (2021)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

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Pendahuluan: Penelitian sebelumnya menyebutkan ekstrak alang-alang berpotensi meningkatkan kerja antibiotik. Belum diketahui senyawa aktif spesifik yang berperan. Tujuan penelitian untuk mendapatkan senyawa aktif dengan metode fraksinasi untuk melihat daya hambat tunggal dan kombinasinya dengan amoxicillin atau chloramphenicol terhadap S. aureus.Metode: Ekstraksi dilakukan dengan maserasi selama 24 jam menggunakan metanol, dipisahkan cair-cair menggunakan heksana, diuapkan pada 55oC. Fraksinasi dilakukan menggunakan aquadest (fraksi 1), etil asetat (fraksi 2) dan methanol (fraksi 3). Uji fitokimia secara kualitatif dengan melihat isi senyawa aktif. Uji Zone of Inhibition (ZOI) dilakukan untuk mengetahui efek kombinasi fraksi fenolik alang-alang dengan antibiotik terhadap S. aureus dengan metode Kirby-Bauer. ZOI diukur menggunakan jangka sorong satuan mm. Interpretasi hasil berdasarkan metode Ameri-Ziaei Double Antibiotic Synergism Test.Hasil: : Pada semua fraksi ditemukan senyawa aktif alkaloid. Kombinasi fraksi 1 (9,26 ± 0,89 mm) dan 3 (7,65 ± 1,88 mm) dengan amoxicillin (9,56 ± 2,37 mm) menunjukkan penurunan zona bening dengan p valeu 0,986 dan 0,175. Fraksi 2 (9,56 ± 1,38 mm) menunjukkan rata-rata yang sama dengan p valeu 1,000. Kombinasi fraksi 1 (13,46 ± 0,23 mm) dengan chloramphenicol (13,26 ± 1,82 mm) menunjukkan peningkatan zona bening dengan p valeu 0,709. Fraksi 2 (10,91 ± 0,31 mm) dan 3 (13,15 ± 0,6 mm) dengan chloramphenicol menunjukkan penurunan zona bening dengan p valeu 0,806 dan 0,851.Kesimpulan: Kombinasi fraksi 1-3 Imperata cylindrica, L. dengan antibiotik amoxicillin atau chloramphenicol terhadap bakteri Staphylococcus memiliki jenis interaksi yang not distinguishable (ND).

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