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ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA) Julianti, Elin; Fathurohman, Mochamad; Damayanti, Sophi; Kartasasmita, Rahmana Emran
Marine Research in Indonesia Vol 43, No 2 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum.

ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA) Julianti, Elin; Fathurohman, Mochamad; Damayanti, Sophi; Kartasasmita, Rahmana Emran
Marine Research in Indonesia Vol 43 No 2 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum.

UJI IDENTIFIKASI ASAM LEMAK TIDAK JENUH DARI MIKROALGA Thraustochytrids MENGGUNAKAN METODE EKSTRAKSI TRANSETERIFIKASI IN SITU Fathurohman, Mochamad; Yuliana, Anna; Aldiany, Elsa; Pratita, Anindita Tri kusuma
Journal of Pharmacopolium Vol 4, No 1 (2021)
Publisher : P3M STIKes Bakti Tunas Husada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36465/jop.v4i1.717

PENINGKATAN KESEHATAN MASYARAKAT MELALUI PENGENALAN DIVERSIFIKASI PRODUK NUTRASETIKAL DI DESA BUNDER JAWA BARAT Fathurohman, Mochamad; Pratita, Anindita Tri Kusuma; Wardani, Gatut Ari; Setiawan, Fajar; Lestari, Tresna; Nofianti, Tita; Ruswanto, Ruswanto; Nurviana, Vera; Rahmiyani, Ira; Nurdianti, Lusi
JMM (Jurnal Masyarakat Mandiri) Vol 5, No 4 (2021): Agustus
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v5i4.5113

Abstrak: Tujuan pengabdian kepada masyarakat ini untuk memberikan informasi tentang pangan fungsional dan suplemen makanan serta memperkenalkan contoh produknya yaitu susu kedelai berbasis mikroalga. Metode kegiatan dalam bentuk sosialisasi dengan cara diskusi dan pembagian produk. Sasaran kepada masyarakat terutama ibu rumah tangga dengan jumlah yang hadir 43 orang. Untuk mengetahui pemahaman peserta dilakukan evaluasi dalam bentuk kuisioner sertatanya jawab terkait materi yang disampaikan. Hasil yang telah dicapai bahwa peserta dapat meningkatkan pengetahuan terkait produk nutrasetikal dengan presentase rerata 80-90%, serta diharapkan dapat membuat atau mengembangkan inovasi produk lainnya.Â Abstract: This community service aims to provide information about functional foods and food supplements and introduce microalgae based soy milk products. Methods in socialization activities by discussion and product distribution. The target for the community, especially recitation participants, with the number of people attending, is about 43 people. To find out the understanding of the evaluation participants in the form of questionnaires and questions and answers related to the material presented. The results can increase knowledge related to nutraceutical products with a precented of 80-90%, and create or develop other product innovations.Â

PENYULUHAN PENCEGAHAN PENYAKIT PASCA BANJIR DENGAN MENERAPKAN POLA PHBS DI DUSUN RANGKASAN Pratita, Anindita Tri Kusuma; Mabruro, *De Hiban Fauzan; Bahri, Heri Syaiful; Amrullah, Muhammad Muzaki Fikri; Rabbani, Dzilla Fadillah; Fauziah, Siska Rahmatul; Jenika, Vika; Fathurohman, Mochamad
Kumawula: Jurnal Pengabdian Kepada Masyarakat Vol 6, No 3 (2023): Kumawula: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kumawula.v6i3.47787

Bencana banjir yang terjadi di Dusun Rangkasan, Desa Tarisi, Kecamatan Wanareja menyebabkan timbulnya penyakit pasca banjir yang dialami oleh masyarakat. Penyakit pasca banjir adalah suatu penyakit yang timbul setelah terjadinya bencana banjir diantaranya penyakit kulit (kurap), diare, demam dan lainnya yang terjadi apabila turunnya hujan deras setiap tahun, yang mengakibatkan air sungai meluap. Penelitian ini bertujuan untuk mengetahui perbandingan tingkat pengetahuan dan memberikan kontribusi dalam pemahaman masyarakat Dusun Rangkasan mengenai cara penanggulangan terhadap penyakit pasca banjir dengan menerapkan pola PHBS. Metode dalam penelitian ini menggunakan One Group Pretest Posttest Design dengan menganalisis data secara Deskriptif. Hasil yang didapatkan dengan adanya perbandingan usia, yaitu antara usia 22-27 tahun diperoleh nilai persentase tertinggi 30,43478 %. Sedangkan hasil dengan perbandingan pendidikan terakhir terdapat dalam pendidikan SMP dengan nilai persentase tertinggi 56,5 %. Dapat disimpulkan terdapat 23 responden yang menghadiri penyuluhan ini dengan adanya perubahan signifikan sangat mempengaruhi terhadap data hasil perbandingan tingkat pengetahuan antar usia dengan pendidikan terakhir berdasarkan pengetahuan sebelum dan sesudah penyampaian materi. The flood disaster that occurred in Rangkasan Hamlet, Tarisi Village, Wanareja District caused the emergence of post-flood diseases experienced by the community. Post-flood disease is a disease that arises after a flood disaster, including skin diseases (ringworm), diarrhea, fevers, and others that occur when there is heavy rain every year, which causes river water to overflow. This study aims to compare the level of knowledge and contribute to the understanding of the people of Rangkasan Hamlet regarding how to deal with post-flood diseases by applying the PHBS pattern. The method in this study used the One Group Pretest Posttest Design by analyzing the data descriptively. The results obtained by comparison of age, namely between the ages of 22-27 years, obtained the highest percentage value of 30.43478%. While the results with the last comparison of education are in junior high school education with the highest percentage value of 56.5%. It can be concluded that there were 23 respondents who attended this counseling with a significant change that greatly affected the data resulting from a comparison of knowledge levels between ages and recent education based on knowledge before and after delivering the material.Bencana banjir yang terjadi di Dusun Rangkasan, Desa Tarisi, Kecamatan Wanareja menyebabkan timbulnya penyakit pasca banjir yang dialami oleh masyarakat. Penyakit pasca banjir adalah suatu penyakit yang timbul setelah terjadinya bencana banjir diantaranya penyakit kulit (kurap), diare, demam dan lainnya yang terjadi apabila turunnya hujan deras setiap tahun, yang mengakibatkan air sungai meluap. Penelitian ini bertujuan untuk mengetahui perbandingan tingkat pengetahuan dan memberikan kontribusi dalam pemahaman masyarakat Dusun Rangkasan mengenai cara penanggulangan terhadap penyakit pasca banjir dengan menerapkan pola PHBS. Metode dalam penelitian ini menggunakan One Group Pretest Posttest Design dengan menganalisis data secara Deskriptif. Hasil yang didapatkan dengan adanya perbandingan usia, yaitu antara usia 22-27 tahun diperoleh nilai persentase tertinggi 30,43478 %. Sedangkan hasil dengan perbandingan pendidikan terakhir terdapat dalam pendidikan SMP dengan nilai persentase tertinggi 56,5 %. Dapat disimpulkan terdapat 23 responden yang menghadiri penyuluhan ini dengan adanya perubahan signifikan sangat mempengaruhi terhadap data hasil perbandingan tingkat pengetahuan antar usia dengan pendidikan terakhir berdasarkan pengetahuan sebelum dan sesudah penyampaian materi. Kata Kunci: Penyakit Pasca Banjir; PHBS; Usia; Pendidikan Terakhir

PENGARUH PENAMBAHAN PEKTIN DARI KULIT PISANG KAPAS (Musa acuminata Colla) TERHADAP MUTU SELAI LEMBARAN MANGGA HARUMANIS (Mangifera indica L.) Tuslinah, Lilis; Rahmawati, Sita; Fathurohman, Mochamad
ARGIPA (Arsip Gizi dan Pangan) Vol 8 No 2 (2023)
Publisher : UHAMKA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/argipa.v8i2.12664

Pectin is a polymer of D-galacturonic acid linked by α-1,4 glycosidic bonds. Pectin is widely used in the pharmaceutical, food, and beverage industries as a gelling agent and stabilizing agent. This study aims to utilize cotton banana skin waste as an alternative source of pectin and determine the levels of pectin added to make the best quality fragrant mango slice jam. Pectin from cotton banana peels was obtained by extraction using HCl solvent with a pH of 2.5 at 100°C for 120 minutes. The yield obtained was 8.67%. The pectin obtained was analyzed to meet the quality requirements for pectin, namely water content 9.85%, ash content 6.73%, equivalent weight 6,460.485 mg, methoxyl content 2.85%, galacturonic content 75.73%, degree of esterification 21.4 % and spectrum analysis Fourier Transform Infrared (FTIR) and the best slice jam product was obtained with the addition of 1.5% pectin. Then the slice jam was analyzed, and the results obtained were 17.55% water content, 84.56% texture analysis, 70% total dissolved solids, and hedonic tests on the organoleptic of slice jam, namely color, aroma, taste, and texture, which, in general, the subject liked fruit slice jam fragrant mango with the addition of 1.5% pectin from cotton banana peels.

Potential of Dimethyl Sulfoxide Modified Kaolin and Cetyl Trimethylammonium Bromide as Amoxycillin Adsorben Wardani, Gatut Ari; Damayanti, Ratih; Fathurohman, Mochamad; Hidayat, Taufik; Nofiyanti, Estin
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 9, No 1 (2024): February 2024
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v9.i1.18

The large use of amoxicillin allows this antibiotic to enter the environment in large quantities and cause pollution. The adsorption technique can be used as a method to remove amoxicillin contaminants in wastewater by utilizing kaolin as an adsorbent. This study aims to determine the characteristics of kaolin as an adsorbent and determine the ability of kaolin to adsorb amoxicillin. Kaolin was activated with hydrochloric acid and modified using Dimethyl sulfoxide (DMSO) and cetyl trimethylammonium bromide (CTAB). The results showed that the characteristics of DMSO and CTAB-modified kaolin showed a new peak which was an O-H bending vibration in functional group analysis using FTIR. X-ray diffraction results show that the distance between planes in the kaolin structure is larger. Meanwhile, the results of the SEM analysis showed that the surface morphology of kaolin had a higher level of crystallinity than before which proved an increase in the adsorption capacity of kaolin. The adsorption kinetics follows the Santosa kinetic equation model with an adsorption rate of 0.004 min-1 and an equilibrium constant value of 0.007 L.mol-1. The adsorption isotherm test follows the Freundlich isotherm equation model with an adsorption constant value of 561.694 L.mg-1 and an empirical constant value of 0.270. Keywords: Antibiotics, Adsorption, Batch, Isotherms, Kinetics

EDUKASI PEMANFAATAN TANAMAN OBAT KELUARGA SEBAGAI UPAYA PENINGKATAN KESEHATAN MASYARAKAT DI JAWA BARAT Fathurohman, Mochamad; Aulia, Mila
JMM (Jurnal Masyarakat Mandiri) Vol 9, No 4 (2025): Agustus
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstrak: Tanaman Obat Keluarga (TOGA) merupakan alternatif pengobatan tradisional yang aman, murah, dan mudah diakses oleh masyarakat. Namun, tingkat pemanfaatannya masih rendah akibat kurangnya pengetahuan masyarakat mengenai jenis, manfaat, dan cara pengolahannya. Kegiatan pengabdian ini bertujuan untuk meningkatkan pemahaman masyarakat terhadap TOGA melalui penyuluhan interaktif di Desa Mekarjaya, Kecamatan Padakembang, Kabupaten Tasikmalaya. Metode yang digunakan meliputi ceramah, diskusi, serta evaluasi menggunakan pre-test dan post-test yang terdiri dari 10 pertanyaan dan diberikan kepada 32 responden. Hasil evaluasi menunjukkan adanya peningkatan pemahaman peserta, dengan rata-rata skor pre-test sebesar 37,81% dan post-test sebesar 74,37%, menunjukkan peningkatan sebesar 36,56% poin. Kegiatan ini membuktikan bahwa penyuluhan berbasis interaksi langsung efektif dalam meningkatkan kesadaran masyarakat akan pentingnya TOGA sebagai solusi pengobatan mandiri berbasis potensi local.Abstract: Family Medicinal Plants (TOGA) serve as a traditional, safe, affordable, and easily accessible alternative medicine. However, community utilization remains low due to limited knowledge regarding the types, benefits, and processing methods. This community service program aimed to enhance public understanding of TOGA through interactive education in Mekarjaya Village, Padakembang District, Tasikmalaya Regency. The method used included lectures, discussions, and evaluations using pre-tests and post-tests consisting of 10 questions and given to 32 respondents. Evaluation results showed a significant improvement in understanding, with the average pre-test score of 37.81% increasing to 74.37% in the post-test, indicating a gain of 36.56% points. This activity demonstrates that interactive educational approaches are effective in raising community awareness regarding TOGA as a locally based self-medication solution.

PENYULUHAN DIVERSIFIKASI NUTRISI PRODUK SEBAGAI UPAYA PENCEGAHAN STUNTING PADA IBU HAMIL Fathurohman, Mochamad; Ramadhani, Melinda Putri; Lucky, Priya Auliyaa; Setiawan, Rio Anggi; Mustika, Shara Liviany; Azzahra, Shofiatunnisa Husna
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 5 (2024): Oktober
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i5.25765

Abstrak: Tujuan penyuluhan dengan konsep pengabdian kepada masyarakat ini untuk memberikan informasi megenai peningkatan nutrisi pada dengan konsep diversifikasi produk dalam upaya mencegah stunting dengan sasaran khusus pada ibu hamil dan umum pada masyarakat luas. Hasil produk yang dihasilkan yaitu olahan makanan kudapan dengan kandungan protein hewani yang sangat tinggi yaitu pudding telur. Metode kegiatan dalam bentuk sosialisasi dan penyuluha dengan cara diskusi dan pembagian produk. Sasaran kepada masyarakat terutama ibu hamil dan Masyarakat umum yang hadir berjumlah 22 orang. Untuk mengetahui pemahaman pentingnya asupan nutrisi seimbang pada peserta dilakukan evaluasi dalam bentuk kuisioner serta tanya jawab terkait materi yang disampaikan. Hasil yang telah dicapai bahwa peserta dapat memahami pentingnya pengetahuan terkait nutrisi seimbang dalam konsep preventif suatu penyakit dan contoh produk diversifikasinya dengan presentase rata-rata 94%, serta diharapkan dapat menerapkan dengan konsisten dalam pemenuhan gizi seimbang dalam pencengahan stunting.Abstract: The purpose of counseling with the concept of community service is to provide information on improving nutrition with the concept of product diversification in an effort to prevent stunting with special targets for pregnant women and the general public. The resulting product is processed snack food with a very high animal protein content, namely egg pudding. The method of activity is in the form of socialization and education by means of discussion and product distribution. The target audience, especially pregnant women and the general public, totaled 22 people. To determine the understanding of the importance of balanced nutritional intake in participants, an evaluation was carried out in the form of questionnaires and questions and answers related to the material presented. The results that have been achieved are that participants can understand the importance of knowledge related to balanced nutrition in the concept of preventing a disease and examples of diversification products with an average percentage of 94%, and are expected to apply consistently in fulfilling balanced nutrition in preventing stunting.

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