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Description Of C-Reactive Protein (CRP) In Patients With Type 2 Diabetes Mellitus At Mulyorejo Health Center In Malang City Febriyanti Wora Kapero; Erni Yohani Mahtuti; Previta Zeizar Rahmawati
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.320

Diabetes Mellitus Type 2 is a metabolic disorder condition characterized by hyperglycemia, due to decreased insulin secretion. Many cytokines play a role in the acute phase response, so that markers such as CRP increase in patients with type 2 DM. The type of research used in this study is descriptive research, which describes the results of CRP examination in patients with type 2 diabetes. Examination of respondents' serum C-Reactive Protein (CRP) levels was carried out qualitatively using the Latex Agglutination method and followed by a semi-quantitative method, namely dilution to determine the level of inflammation. The results of CRP examination on 30 serum samples that have been carried out on patients with Type 2 Diabetes Mellitus found that 29 (96.7%) were positive and 1 (3.3%) of respondents were negative. The group of CRP levels for titer 12 was 5 samples (16.7%), titer 24 was 17 samples (56.7%), titer 48 was 5 samples (16.7%), and titer 96 was 2 samples (6.7). %). The results of the four groups with the highest CRP levels, namely a titer of 24 mg/L as many as 17 samples (56.7%) showed that some of the CRP levels were higher than normal. Conclusion Almost all respondents with type 2 Diabetes Mellitus had positive C-Reactive Protein examination results and a small proportion of type 2 Diabetes Mellitus patients had negative C-Reactive Protein examination results.

Utilization Of Coconut Dregs As An Alternative Medium For The Growth Of Bacillus Subtilis And Escherchia Coli Dewita Nggua; Erni Yohani Mahtuti
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.331

Coconut dregs are waste produced after processing coconuts into coconut oil. Coconut pulp has a high nutritional content which can be used for bacterial growth. The nutritional content of coconut dregs is 38.1% carbohydrates, 5.6% crude protein, 16.3% crude fat, 31.6% crude fiber, 2.6% ash content and 5.5% water content. The research used coconut dregs as an alternative medium for the growth of gram-negative and gram-positive bacteria. In the trials, B. subtilis and E. coli bacteria were used. The type of research used was a true experiment with a completely randomized design. The results of the research showed that the highest average number of colonies on the coconut dregs alternative media was in treatment A1, namely the average of B.subtilis colonies was 50.6 x 10² CFU/mL and E.coli 36 .6 x 10² CFU/mL while the control media averaged B.subtilis colonies 75 x 10² CFU/mL and E.coli 86.3 x 10² CFU/mL. The results of statistical tests using anova p<0.05 with a sig value of 0.001 show that there is an influence of differences in the concentration of coconut dregs and agar as an alternative medium for bacterial growth. The post hoc test compares the concentration between treatment A1 and control with a sig p value of >0.05 (0.250), namely There is no significant difference, while the concentration between treatments A2, A3 and A4 with control has a sig p value <0.05, namely 0.000, meaning there is a significant difference. So treatment A1 is the best treatment for the growth of B.subtilis and E.coli bacteria.

Utilization Of Coconut Dregs As An Alternative Medium For The Growth Of Bacillus Subtilis And Escherchia Coli Nggua, Dewita; Mahtuti, Erni Yohani
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.331

Coconut dregs are waste produced after processing coconuts into coconut oil. Coconut pulp has a high nutritional content which can be used for bacterial growth. The nutritional content of coconut dregs is 38.1% carbohydrates, 5.6% crude protein, 16.3% crude fat, 31.6% crude fiber, 2.6% ash content and 5.5% water content. The research used coconut dregs as an alternative medium for the growth of gram-negative and gram-positive bacteria. In the trials, B. subtilis and E. coli bacteria were used. The type of research used was a true experiment with a completely randomized design. The results of the research showed that the highest average number of colonies on the coconut dregs alternative media was in treatment A1, namely the average of B.subtilis colonies was 50.6 x 10² CFU/mL and E.coli 36 .6 x 10² CFU/mL while the control media averaged B.subtilis colonies 75 x 10² CFU/mL and E.coli 86.3 x 10² CFU/mL. The results of statistical tests using anova p<0.05 with a sig value of 0.001 show that there is an influence of differences in the concentration of coconut dregs and agar as an alternative medium for bacterial growth. The post hoc test compares the concentration between treatment A1 and control with a sig p value of >0.05 (0.250), namely There is no significant difference, while the concentration between treatments A2, A3 and A4 with control has a sig p value <0.05, namely 0.000, meaning there is a significant difference. So treatment A1 is the best treatment for the growth of B.subtilis and E.coli bacteria.

Description Of C-Reactive Protein (CRP) In Patients With Type 2 Diabetes Mellitus At Mulyorejo Health Center In Malang City Kapero, Febriyanti Wora; Mahtuti, Erni Yohani; Rahmawati, Previta Zeizar
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.320

Diabetes Mellitus Type 2 is a metabolic disorder condition characterized by hyperglycemia, due to decreased insulin secretion. Many cytokines play a role in the acute phase response, so that markers such as CRP increase in patients with type 2 DM. The type of research used in this study is descriptive research, which describes the results of CRP examination in patients with type 2 diabetes. Examination of respondents' serum C-Reactive Protein (CRP) levels was carried out qualitatively using the Latex Agglutination method and followed by a semi-quantitative method, namely dilution to determine the level of inflammation. The results of CRP examination on 30 serum samples that have been carried out on patients with Type 2 Diabetes Mellitus found that 29 (96.7%) were positive and 1 (3.3%) of respondents were negative. The group of CRP levels for titer 12 was 5 samples (16.7%), titer 24 was 17 samples (56.7%), titer 48 was 5 samples (16.7%), and titer 96 was 2 samples (6.7). %). The results of the four groups with the highest CRP levels, namely a titer of 24 mg/L as many as 17 samples (56.7%) showed that some of the CRP levels were higher than normal. Conclusion Almost all respondents with type 2 Diabetes Mellitus had positive C-Reactive Protein examination results and a small proportion of type 2 Diabetes Mellitus patients had negative C-Reactive Protein examination results.

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