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MORPHOLOGICAL IDENTIFICATION OF FUNGAL PATHOGENS ASSOCIATED WITH POST-HARVEST DISEASES Ulilalbab, Azizah Ridha; Cahyasita, Dhika; Pratiwi, Nova Wahyu; Ajri, Miftahul; Hanikaf, Uliyatul Muhmiroh; Kuncoro, Seto Agung
Jurnal Agrivet Vol 29 No 2 (2023): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v29i2.11001

Abstract

Post-harvest products are perishable and vulnerable to diseases that lead to quality deterioration and yield loss. One of the primary diseases found in most post-harvest products is caused by fungal pathogens. This study identified fungal pathogens associated with post-harvest products through morphological characterization. Fruit and vegetable samples were collected from traditional markets and fruit stores in Central Java. The results of fungal pathogens identification causing disease on post-harvest products showed that Pestalotiopsis sp and Neopestalotiopsis sp. were found on guava with white blackish mycelium, present concentric ring and blackspot, Aspergillus sp. on tomato with yellow-greenish mycelium, present concentric ring only, Botrytis sp. with grey mycelium and Rhizoctonia solani with white greyish mycelium and present of a concentric ring on apple, Rhizoctonia solani with greyish black and present of blackspot on mango, and Colletotrichum sp. with white greyish mycelium and present of a concentric ring, conidiomata and blackspot on citrus. This study concluded that the most fungal pathogens on post-harvest that we found were Pestaloptia sp., Rhizoctonia solani, Aspergillus sp., Botrytis sp., and Colletotrichum sp.
Diseases and Pests Associated with Avocado Plantation Intercropping with Mango and Durian in Watulembu, Wonogiri Poerwanto, Mofit Eko; Wicaksono, Danar; Ulilalbab, Azizah Ridha; Ajri, Miftahul
Jurnal Agrivet Vol 30 No 2 (2024): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v30i2.13049

Abstract

One of strategic fruit commodities was avocado that had high economic value. Pest and diseases were main problems faced by farmers. This study identified diseases and pest associated with avocado plantation intercropping with mango and durian in Watulembu district, Wonogiri Regency through symptoms and its controls. The observation was done to the abnormalities avocado plants and the field condition surrounding the planting area. It was conducted by visual observation, recorded using a digital camera, and then described and identified based on their symptoms and a literature review. The results of our observation were Algal leaf spot, Anthracnose, Sooty mold, and Planococcus citri. Pest and plant disease could be controlled by cultural technique, avoid plant stress, and also using chemical pesticide.
Repellent Ability of Encapsulated Guava Leaves Extract and Horticultural Mineral Oil (HMO) on the Feeding Behaviour of Diaphorina citri Poerwanto, Mofit Eko; Danar, Wicaksono; Ajri, Miftahul; Ulilalbab, Azizah Ridha
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.455-465

Abstract

Repellency is one of strategies to reduce Diaphorina citri attack. Guava leaf extract (GLE) and horticultural mineral oil (HMO) are well-known as repellents against D. citri but have short persistence. This research was conducted to increase the persistence of GLE and HMO extracts by using simultaneous co-delivery as part of environmentally friendly control of D. citri. The treatments used were 5% GLE, 5% HMO, 5% GLE + 5% HMO, 5% encapsulated GLE, 5% encapsulated HMO, 5% encapsulated GLE + 5% encapsulated HMO, and aquadest as control. The result shows that 5% HMO, 5% GLE + 5% HMO, and 5% encapsulated GLE have a repellency effect on D. citri. In the non-encapsulated treatments, the percentage of D. citri stayed at treated citrus leaves increased significantly at 9 and 12 hours after application except 5% GLE + 5% HMO. Exposure to 5% GLE, 5% HMO, and 5% encapsulated GLE. The most dominant of GLE compounds was lactose and d-Glycero-l-gluco-heptose. The most dominant of HMO compounds were 17-Pentatriacontene, Tetrapentacontane 1,54-dibromo- and tert-Hexadecanethiol. Encapsulation of 5% GLE can increase the persistence of GLE as a repellent compound for D. citri, but this effect does not occur in HMOs.
IDENTIFIKASI SECARA MORFOLOGI DAN MORFOMETRI NEMATODA ENTOMOPATOGEN PADA LAHAN PERTANAMAN JAGUNG DI KABUPATEN GUNUNGKIDUL Kholilurrahman Fathani, Kholil; Ajri, Miftahul
Jurnal Agrivet Vol 31 No 3 (2025): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v31i3.15952

Abstract

Corn productivity often declines due topest attacks, but can be controlled through the use of biological agents such asentomopathogenic nematodes (EPN). This study aims to explore andidentify EPN in corn fields in Gunungkidul Regency morphologically and morphometrically. Soil samples were taken in five districts, namely Wonosari, Playen, Patuk, Karangmojo, and Semanu. NEP trapping was carried out using Tenebrio molitor larvae, then isolatedusing the white head tray method and observed morphologically and morphometrically using a microscope. The results showed that the highest NEP population NEP population was found in Playen Subdistrict (462.67 NEP/5 ml) and the lowest in Patuk Subdistrict (7.33 NEP/5 ml). Population variation is thought to be influenced by differences in soil texture and cultivation systems. Based on the morphological and morphometric characteristics of the NEP found in Gunungkidul corn fields, they were identifiedas Heterorhabditis indica, with a body length ranging from 438.77–501.9 µm. These findings indicate the potential of H. indica as a biological agentin pest control in corn farming ecosystems in the Gunungkidul. Keywords : morphology, morphometry, population, Tenebrio molitor, white head tray
Exploration of Entomopathogenic Nematodes in Organic Rice Field in Sleman Regency Miftahul Ajri; Dinda Dewi Aisyah; Azizah Ridha Ulilalbab
SEAS (Sustainable Environment Agricultural Science) Vol. 7 No. 2 (2023)
Publisher : Warmadewa University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/seas.7.2.8266.153-160

Abstract

Entomopathogenic nematodes (EPNs), consisting of genera Steinernema and Heterorhabditis are biological agents proven effective in killing insect pests. Organic rice fields and sandy soil textures are potential areas for exploring the presence of EPNs. This research aims to determine the diversity of genera and populations of entomopathogenic nematodes in organic rice fields in Sleman Regency. The study was conducted on organic rice fields and the UPN "Veteran" Yogyakarta Plant Protection Laboratory from July – August 2023. EPNs were obtained from Prambanan Sleman and Ngemplak Sleman, with six plots as sample units in every location. EPNs were isolated from soil samples by using Tenebri molitor larvae. The Whitehead tray method was used to isolate EPNs from the dead larvae, and the population of EPNs was counted. EPNs were identified based on the symptoms of color changes on the cuticle and morphological characters. The result showed that the cuticles of T. molitor larvae that died were blackish brown in Prambanan and Ngaliyan isolates, referred to Steinernema genera. EPNs were found in Prambanan_6, Ngaliyan_1, and Ngaliyan_2. The highest population of EPNs was on Ngaliyan_1 (38,00 EPNs/ml), and the lowest was on Prambanan_6 (2,13 EPNs/ml). Based on the morphological characters of EPNs, on the Prambanan_4, Ngaliyan_1, and Ngaliyan_2, EPNs were identified as Steinernema spp. The characteristics of Steinernema in the infective juvenile stage found are: slender body, anterior end slightly rounded and fused with the body, cylindrical stoma, no second cuticular sheath, not annulated, and conoid tail.
Kelimpahan Nematoda Parasit dan Nonparasit pada Tanaman Pisang (Musa paradisiaca L.) di Kabupaten Sleman dan Bantul, Daerah Istimewa Yogyakarta Miftahul Ajri; Azizah Ridha Ulilalbab; Mofit Eko Poerwanto; Danar Wicaksono; Ayu Kumala Sari; Septi Sri Rahmawati
Jurnal Ilmu-Ilmu Pertanian Vol. 33 No. 1 (2026): Jurnal Ilmu Ilmu Pertanian
Publisher : Politeknik Pembangunan Pertanian Yogyakarta-Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55259/jiip.v33i1.400

Abstract

Tanaman pisang (Musa paradisiaca L.) merupakan komoditas hortikultura penting yang produktivitasnya sering menurun akibat serangan nematoda parasit pada sistem perakaran. Penelitian ini bertujuan untuk mengidentifikasi nematoda parasit dan nonparasit yang berasosiasi dengan tanaman pisang di beberapa wilayah Kabupaten Sleman dan Bantul, Daerah Istimewa Yogyakarta. Sampel tanah dan akar tanaman pisang diambil dari lima lokasi, yaitu Turi, Pakem, Ngaglik, Condongcatur, dan Banguntapan. Ekstraksi nematoda dilakukan menggunakan metode Whitehead tray yang dimodifikasi, sedangkan identifikasi dilakukan berdasarkan karakter morfologi dengan menggunakan mikroskop. Data faktor lingkungan seperti pH tanah, kelembapan, suhu, ketinggian tempat, dan koordinat lokasi digunakan sebagai data pendukung dalam analisis deskriptif. Hasil pengamatan menunjukkan bahwa keberadaan nematoda parasit dan nonparasit pada sampel tanah dan akar bervariasi antarlokasi. Nematoda parasit didominasi oleh Pratylenchus spp. dan Helicotylenchus spp., dengan populasi tertinggi ditemukan di Ngaglik pada sampel tanah maupun akar. Genus Pratylenchus spp. dominan ditemukan pada sampel akar, sedangkan Helicotylenchus spp. lebih banyak ditemukan pada sampel tanah. Nematoda nonparasit yang ditemukan meliputi Mononchus sp., Steinernema sp., Rhabditis sp., dan Metarhabditis sp. Keberadaan Mononchus sp. menunjukkan potensi interaksi predator–mangsa dalam komunitas nematoda tanah, sedangkan Steinernema sp. berpotensi sebagai agen pengendali hayati. Variasi populasi nematoda antarlokasi diduga dipengaruhi oleh faktor lingkungan seperti pH tanah, kelembapan, suhu, dan ketinggian tempat. Penelitian ini memberikan informasi dasar mengenai keberadaan nematoda pada pertanaman pisang di Kabupaten Sleman dan Bantul yang dapat dimanfaatkan sebagai acuan dalam pengelolaan nematoda secara berkelanjutan.