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Aktivitas Antioksidan dan Penghambatan Enzim α-amilase Ekstrak Air Kombinasi Daun Papasan (Coccinia grandis [L.]) dan Daun Sembung (Blumea balsamifera [L.] DC) Atikawati, Atikawati; Kusumawati, I Gusti Ayu Wita; Putra, I Made Wisnu Adhi; Yogeswara, Ida Bagus Agung
Jurnal Media Sains Vol 3, No 2 (2019): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.915 KB) | DOI: 10.36002/jms 3.v3i2.911

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui aktivitas antioksidan dan penghambatan ?-amilase pada kombinasi ekstrak air daun papasan dan daun sembung. Ekstrak dibuat dengan cara menyeduh serbuk daun papasan dan daun sembung dengan berbagai rasio (1:3, 1:1, dan 3:1). Penyeduhan dilakukan menggunakan 100 ml air mendidih (100?C) selama 5 menit. Hasil penelitian menunjukkan bahwa aktivitas antioksidan dalam menangkal radikal bebas DPPH tertinggi terdapat pada sampel kombinasi 1:3 yang dinyatakan dengan IC50 sebesar 1,58?0,06 mg/ml danaktivitas penghambatan ?-amilase tertinggi juga terdapat pada sampel kombinasi rasio 1:3 dengan nilai IC50 sebesar 1,29?0,02 mg/ml. Secara umum, semua kombinasi berpotensi sebagai minuman fungsional antidiabetes.Kata kunci: Daun papasan, daun sembung, aktivitas antioksidan, aktivitas penghambatan ?-amilase, ekstrak air.ABSTRACTThis study aims to determine the antioxidant and inhibition of ?-amylase activities of the combination of Coccinia grandis [L.] and Blumea balsamifera [L.] DC water extracs. The extracts wass made by decoctingthe leaves powder in various ratios (1: 3, 1: 1, and 3: 1). Decoction process was done using 100 ml of infuced water (100?C) for 5 minutes. The results showed that thethe highest antioxidant activity in counteracting free radicals DPPH was found in combination of 1:3 expressed by IC50 of 1.58?0.06 mg/ml and the highest ?-amylase inhibitory activity was also found in combination of 1:3sample with IC50 values of 1.29?0.02 mg/ml. In general, all combinations have potential to be antidiabetic functional drinks.Keywords: Coccinia grandis [L.], Blumea balsamifera [L.] DC, antioxidant and inhibition of ?-amylase, water extract.
Efektifitas sinar ultraviolet terhadap cemaran bakteri patogen pada makanan cair sonde untuk pasien immune-compremissed Sulatri, Ni Luh; Yogeswara, Ida Bagus Agung; Nursini, Ni Wayan
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 5, No 2 (2017)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.018 KB) | DOI: 10.14710/jgi.5.2.112-118

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Background : Food safety remained as critical concern to immune-compremissed patient. Food safety assurance can be achieved through inhibition of pathogenic bacteria by physical treatment such as UV light radiation. However, a study regarding the effect of UV light on growth of pathogenic bacteria in contaminated liquid food are scarce.Objective : To determine the effectiveness of UV light on contamination of pathogenic bacteria in liquid food for immune-compremissed patient.Methods : Randomized design with two factor which were holding time for 60 and 120 minutes and radiation exposure (0, 5, 10 and 15 minutes). The data was analyzed using ANOVA Result : The viscosity and pH of liquid foods were 20 centipoise and 7,15 respectively. Radiation of UV light on contaminated food that have been incubated for 60 and 120 minutes at 37oC showed significant increase (1-2 log cycle) on growth of pathogenic bacteria.Conclusion : Radiation of UV light on contaminated liquid food were not effective to inhibit or kill pathogenic bacteria during holding time (60 and 120 minutes).
Tingkat konsumsi zat besi (Fe), seng (Zn) dan status gizi serta hubungannya dengan prestasi belajar anak sekolah dasar Wadhani, Luh Putu Prema; Yogeswara, Ida Bagus Agung
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 5, No 2 (2017)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.069 KB) | DOI: 10.14710/jgi.5.2.82-87

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Background : The main problem faced by Indonesia especially in the education field is the achievement of learning in children is still low in Elementary School. Several factor that influence the learning achievement in children are internal factors, external factors and the learning methods. Nutritional status, sufticient iron (Fe) and zinc (Zn) intake is one of the important indicators to improve the growth and development of brain in children.Objective :The purpose of this study to determine the correlation between level of iron and zinc consumption and nutritional status with children’ learning achievement in Elementary School no. 1 Buduk and Elementary School no. 2 Abianbase.Methods : design of this study was used an Observational Analytics with Cross Sectional Design which has been implemented in April-May 2017 with Simple Random Sampling Technique in the 5th grade students (57 samples).Result : The result of this study showed that most of the iron (Fe) and zinc (Zn) consumption in medium category that is 64,9% and 42,1%, while the nutritional status in normal category that is 64,9%  and the learning achievement includes in good category that is 84,2%. The learning achievement related to iron (Fe) and nutritional status (p=0,000) and not associated with the level of zinc (Zn) consumption (p=0,447).Conclusion : The study showed a correlation between the level of iron (Fe) consumption and nutritional status with learning achievement and there is no correlation between the level of zinc (Zn) consumption.
Pengembangan pangan fungsional berbasis tepung okara dan tepung beras hitam (Oryza sativa L. indica) sebagai makanan selingan bagi remaja obesitas Adiari, Ni Wayan Lisa; Yogeswara, Ida Bagus Agung; Putra, I Made Wisnu Adhi
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 6, No 1 (2017)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.13 KB) | DOI: 10.14710/jgi.6.1.51-57

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Background : Obesity is an excessive build up of fat in the body thus causing weight far above normal and it can improve the risk of degenerative dissease. Increasing food consumption of high protein, fiber and antioxidants could  solve obesity problem. Soyabean milk waste product (okara) has high protein content but low economic value. Black rice flour has high antosianin, antioxidan and fiber. Functional food with main composition okara and black rice flour could  be an alternative as a  functional food for this matter.Objective : To investigate right formulation of snack bar based on nutritional content and  sensory caracteristic for obese adolescent.Methods : This type of research is an experimental study using Complete Randomized Design with three treatments (Formula I, II, and III) and performed two replications (duplo). Nutritional value data between group  was analyzed using Analysis of Variance (ANOVA) while sensory characteristic were tested by calculating hedonic quality test score.Result : Statistical analysis of nutritional content showed there were significantly different between group (p<0.05), but for antioxidants and phenolics levels and analysis of the sensory quality test were not significantly different (p>0.05). Formula I was most preferred and favored by the panelists with energy content 125.64 g, 9.89% water, 3.13% ash, 19.6% protein, 12.41% fat, 45.07% Carbohydrate, 16.44% total sugar, 15.58 % crude fiber, 208.35 mg/L GAEAC antioxidant, 1.01 mg/100 g anthocyanin and 61.05 mg/100 GAE phenolic.Conclusion : Formula I was the most preferred snack bar by panelist. This formula recommended for obese adolescent.
VIABILITAS DAN STABILITAS BAKTERI PROBIOTIK L. acidophillus FNCC 0051 PADA SUSU KEDELAI FERMENTASI SELAMA DI SALURAN CERNA IN VITRO DAN PENYIMPANAN Yogeswara, Ida Bagus Agung; Kusumawati, I Gusti AYu Wita; Nursini, Ni Wayan
Prosiding Seminar Nasional MIPA 2014: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2014
Publisher : Prosiding Seminar Nasional MIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bakteri asam laktat merupakan mikroorganisme yang berperan penting dalam fermentasi pangan dan memberikan efek kesehatan terhadap yang mengkonsumsi. Untuk menjadi organisme probiotik, bakteri asam laktat harus bertahan selama di saluran cerna dan memiliki stabilitas dan viabilitas yang baik selama penyimpanan. Tujuan dari penelitian ini adalah untuk mempelajari viabilitas dan stabilitas L. acidophillus FNCC 0051 pada susu kedelai fermentasi selama di saluran cerna in vitro dan penyimpanan. Strain L. acidophillus FNCC 0051 memiliki viabilitas dan stabilitas yang baik selama 10 hari penyimpanan pada suhu 50 C dan 250 C. Strain L. acidophillus FNCC 0051 mengalami penurunan 1,9 log cycle pada simulated gastric juice selama 10 hari penyimpanan dan di fase simulated intestinal juice. Nilai total asam dan pH susu kedelai fermentasi menunjukkan peningkatan yang signifikan selama 10 hari penyimpanan yaitu 2.5% dan 3,4 berturut turut. Penelitian ini menunjukkan bahwa strain L. acidophillus FNCC 0051 memiliki viabilitas dan stabilitas yang baik selama di saluran cerna dan penyimpanan.Kata-kata kunci: probiotik, susu kedelai fermentasi, penyimpanan, simulasi saluran cerna in vitroAbstract: Lactic acid bacteria as probiotic organism play an important role in food fermentation and gives beneficial effect to the host. In order to be a probiotic organism, lactic acid bacteria must survive through the digestive tract and has a good stability and viability during storage and under simulated gastrointestinal conditions. The aim of this research is to investigate viability and stability of L. acidophillus FNCC 0051 in fermented soymilk during storage. L. acidophillus FNCC 0051 in fermented soymilk has a good viability and stability during 10 days of storage at 50 C and 250 C. L. acidophillus FNCC 0051 decreased 1,9 log cycle in simulated gastric juice during 10 days of storage as well as in simulated intestinal juice. The acidity and pH of fermented soymilk exhibited a significant increase during 10 days of storage i.e. 2.5% and 3.4 respectively. This research has shown that L. acidophillus FNCC 0051 has a good viability and stability in fermented soymilk during storage and under simulated gastrointestinal conditions.Keyword: probiotic, fermented soymilk, storage, simulated gastrointestinal juice
PEMANFAATAN LOLOH SEMBUNG (BLUMEA BALSAMIFERA) SEBAGAI WELCOME DRINK I Gusti Ayu Wita Kusumawati; Ida Bagus Agung Yogeswara
Jurnal Pariwisata Vol 7, No 2 (2020): Jurnal Pariwisata
Publisher : LPPM Universitas Bina Sarana Informatika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.593 KB) | DOI: 10.31294/par.v7i2.8288

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ABSTRAK Loloh sembung merupakan salah satu minuman tradisional bali yang memiliki efek kesehatan sebagai antihipertensi dan mengobati demam. Loloh sembung kurang populer di masyarakat karena rasanya yang pahit, sehingga perlu dilakukan pengembangan dan uji organoleptik dari loloh sembung. Tujuan dari penelitian ini adalah mempelajari karakteristik organoleptik loloh sembung yang telah dimodifikasi. Penelitian dilakukan dengan metode kuantitatif menggunakan kuesioner dan uji organoleptik yang meliputi rasa, warna, tekstur dan aroma. Analisis statistik menggunakan uji chi-square. Penambahan gula kelapa pada loloh sembung secara signifikan lebih disukai oleh panelis dibandingkan loloh sembung tanpa pemanis. Hasil korelasi Spearman menunjukkan bahwa usia panelis memberikan korelasi yang positif terhadap warna dan rasa loloh sembung masing-masing sebesar 0,046 dan 0,092. Sedangkan aroma dan tekstur loloh sembung memberikan nilai korelasi negatif sebesar -0,069 dan -0,011. Hasil ini menunjukkan bahwa usia panelis memiliki pengaruh kesukaan terhadap loloh sembung. Loloh sembung memiliki potensi untuk dapat dikembangkan sebagai welcome drink yang dapat dijadikan ikon suatu daerah wisata di Bali. Kata kunci: Loloh Sembung, Welcome Drink, Minuman Tradisional, Uji Organoleptic Abstract Loloh sembung is a traditional herbal Balinese drink that possess antihypertensive effect  and use to cure fever. Loloh sembung is less popular in the society due to unpleasant taste and thus, it is necessary to develop and conduct an organoleptic test of loloh sembung. The purpose of this study was to study the level of preference from panelists on modified loloh sembung. Quantitative method was performed and the data was collected using questionnaires and organoleptic tests which including taste, color, texture and aroma. The data was statistically tested using chi-square test. The Addition of coconut sugar into loloh sembung was significantly preferred by panelists compared to unsweetened loloh sembung. The results of the Spearman correlation showed that the age of panelist gave a positive correlation with color and taste of loloh  sembung were 0.046 and 0.092 respectively. In contrast, aroma and texture showed negative correlation values of -0.069 and -0.011 respectively. These results indicate that the ages factor of the panelist affects the preference of loloh sembung. The studied suggest that loloh  sembung has a potential to be developed as a welcome drink that can be used as an icon of tourism in Bali. Keywords: Loloh Sembung; Welcome Drink; Traditional Drink; Organoleptic Test
Identification of ɤ-Aminobutyric Acid (GABA) in Loloh as A Potential Drink as Antihipertension I Gusti Ayu Wita Kusumawati; Ni Made Indri Sugiantari; I Gede Ariyasa; Ida Bagus Agung Yogeswara
Majalah Obat Tradisional Vol 23, No 1 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.868 KB) | DOI: 10.22146/mot.28516

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Loloh sembung (Blumea balsamifera) is a functional drink that is widely consumed by Balinesse people and exhibit health-promoting effect. The purpose of this study was to identify GABA of loloh sembung which has physiological effect as antihypertensive. Proximate analysis was performed to determine nutritional value content of loloh sembung. Atomic Absorption Spectrophotometric (AAS) method was employed to determine potassium and sodium content, and identificatio of ɤ-aminobutyric acid (GABA) using Thin Layer Chromatography (TLC) method. In this study, water content of loloh sembung was 99.2324%, ash 0.0117. Fresh sembung leaves have water content 83.8112%, ash content 2.0617%, protein 0.9659%, fat 2.850%, and carbohydrate 10.3109%. Loloh sembung contained sodium, 51.65 mg/L and potassium 1353.10 mg/L. Subsequently, fresh sembung leaves contained sodium 579.72 mg/L and pottasium 1353.19 mg/L respectively. In this study, GABA was detected in loloh sembung and has promising prospect as antihypertensive-functional drink.
In Vitro ACE Inhibitory Activity and Bioactive Compounds of Aqueous Extract of Citrus amblycarpa I Gusti Ayu Wita Kusumawati; I Made Wisnu Adhi Putra; Ida Bagus Agung Yogeswara
Majalah Obat Tradisional Vol 26, No 2 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.65028

Abstract

Citrus amblycarpa contain flavonoid-rich compounds and play important role in suppressing the conversion of angiotensin-converting enzyme 1 (ACE 1) to angiotensin-enzyme 2 (ACE 2). This study aimed to determine the bioactive compounds in the lime peel extract and their ability as in vitro ACE inhibitor activity. The lime peel extract was obtained by boiling the dried peel and dried leaves for 7 min at 70oC. The bioactive compound of the peel and the leaves were compared. The total phenolic, quercetin, rutin, and GABA were further quantified using spectrophotometer UV vis. The aqueous extract of C. ambylcarpa peel showed a high concentration of phenolic, quercetin, rutin, and GABA than that of the leaves extract. Furthermore, the peel extract at low concentration (0.0001 g/mL) has high efficiency in inhibiting ACE activity up to 133%. It can be concluded that the peel of C. amblycarpa is a good candidate for the management of hypertension.
VIABILITAS DAN STABILITAS BAKTERI PROBIOTIK L. acidophillus FNCC 0051 PADA SUSU KEDELAI FERMENTASI SELAMA DI SALURAN CERNA IN VITRO DAN PENYIMPANAN Ida Bagus Agung Yogeswara; I Gusti AYu Wita Kusumawati; Ni Wayan Nursini
Prosiding Seminar Nasional MIPA 2014: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2014
Publisher : Prosiding Seminar Nasional MIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bakteri asam laktat merupakan mikroorganisme yang berperan penting dalam fermentasi pangan dan memberikan efek kesehatan terhadap yang mengkonsumsi. Untuk menjadi organisme probiotik, bakteri asam laktat harus bertahan selama di saluran cerna dan memiliki stabilitas dan viabilitas yang baik selama penyimpanan. Tujuan dari penelitian ini adalah untuk mempelajari viabilitas dan stabilitas L. acidophillus FNCC 0051 pada susu kedelai fermentasi selama di saluran cerna in vitro dan penyimpanan. Strain L. acidophillus FNCC 0051 memiliki viabilitas dan stabilitas yang baik selama 10 hari penyimpanan pada suhu 50 C dan 250 C. Strain L. acidophillus FNCC 0051 mengalami penurunan 1,9 log cycle pada simulated gastric juice selama 10 hari penyimpanan dan di fase simulated intestinal juice. Nilai total asam dan pH susu kedelai fermentasi menunjukkan peningkatan yang signifikan selama 10 hari penyimpanan yaitu 2.5% dan 3,4 berturut turut. Penelitian ini menunjukkan bahwa strain L. acidophillus FNCC 0051 memiliki viabilitas dan stabilitas yang baik selama di saluran cerna dan penyimpanan.Kata-kata kunci: probiotik, susu kedelai fermentasi, penyimpanan, simulasi saluran cerna in vitroAbstract: Lactic acid bacteria as probiotic organism play an important role in food fermentation and gives beneficial effect to the host. In order to be a probiotic organism, lactic acid bacteria must survive through the digestive tract and has a good stability and viability during storage and under simulated gastrointestinal conditions. The aim of this research is to investigate viability and stability of L. acidophillus FNCC 0051 in fermented soymilk during storage. L. acidophillus FNCC 0051 in fermented soymilk has a good viability and stability during 10 days of storage at 50 C and 250 C. L. acidophillus FNCC 0051 decreased 1,9 log cycle in simulated gastric juice during 10 days of storage as well as in simulated intestinal juice. The acidity and pH of fermented soymilk exhibited a significant increase during 10 days of storage i.e. 2.5% and 3.4 respectively. This research has shown that L. acidophillus FNCC 0051 has a good viability and stability in fermented soymilk during storage and under simulated gastrointestinal conditions.Keyword: probiotic, fermented soymilk, storage, simulated gastrointestinal juice
Pemberdayaan Menu Sebagai Upaya Pengentasan Gizi Buruk Dan Obesitas Balita Di Desa Catur, Kintamani, Bangli, Bali Ni Ketut Wiradnyani; Ni Wayan Nursini; I Gede Mustika; Ida Bagus Agung Yogeswara; I Gusti Ayu Wita Kusumawati; Ni Putu Eny Sulistyadewi; Purwaningtyas Kusumaningsih; Dylla Hanggaeni Dyah Puspaningrum
Bhakti Persada Jurnal Aplikasi IPTEKS Vol 7 No 1 (2021): Mei 2021
Publisher : P3M Politeknik Negeri Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31940/bp.v7i1.2397

Abstract

Pengabdian masyarakat prodi Gizi Universitas Dhyana Pura dilakukan di Desa Catur, Bangli Bali bertujuan sebagai upaya memberikan edukasi dan skill kepada masyarakat ibu asuh yang mempunyai anak balita, dalam rangka memperdayakan menu sebagai upaya pengentasan gizi kurang dan lebih. Program ini diberikan untuk mengatasi masalah yang diprioritaskan yaitu: 1) kurangnya pengetahuan para ibu asuh balita mengenai bahan-bahan makanan yang mempunyai potensi untuk meningkatkan maupun menurunkan berat badan, 2) keterbatasan ketersediaan alat dan kurangnya pemahaman mengenai pengolahan makanan menjadi menu yang dapat memperbaiki status gizi anak balita, 3) kurangnya pengetahuan mengenai jenis-jenis menu sebagai asupan gizi yang harus diberikan kepada anak-anak balita gizi kurang dan lebih, 4) kurangnya kemampuan mengolah makanan menjadi menu yang menarik berbasiskan bahan lokal yang sudah ada dan melimpah, 5) kurangnya ketrampilan mengolah makanan dengan menu yang sederhana dalam waktu yang sangat singkat untuk pemenuhan gizi seimbang anak asuh balita. Pengabdian dilakukan dengan metode learning by doing, memberikan materi ceramah diikuti dengan praktek pembuatan menu sebagai upaya pemenuhan gizi seimbang balita yang diikuti oleh ibu asuh balita Desa Catur. Hasil yang diperoleh adalah peningkatan pengetahuan dan skill dalam pembuatan menu gizi seimbang.