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INDONESIA
JURNAL ILMIAH PLATAX
Published by Universitas Sam Ratulangi
ISSN : 23023589     EISSN : -     DOI : -
Core Subject : Social,
Environmental Science
Mencakup Penulisan yang berhubungan dengan pelaksanaan penelitian yang dilakukan secara mandiri, atau kelompok, dan berdasarkan Ruang Lingkup Pengelolaan Wilayah Pesisir, Konservasi, Ekowisata, dan Keanekaragaman Hayati Perairan.
Arjuna Subject : -
Articles 488 Documents
 19   20   21   22   23 
Lift Net Fisheries an it’s Continuity In Local Fishermen Community In North Sulawesi Province (Case study in Labuan Uki Bolaang Mongondow) Ridwan Lasabuda; Lawrence J. L. Lumingas; Rose O. S. E Mantiri
Jurnal Ilmiah PLATAX Vol. 2 No. 1 (2014): Edisi Januari - April 2014
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.2.1.2014.4407

Abstract

Penelitian ini mendeskripsikan aktivitas kehidupan komunitas nelayan tangkap lokal yang beroperasi di kawasan teluk Labuan Uki, Kabupaten Bolaang Mongondow (pesisir Utara  perairan  Sulawesi Utara, Laut Sulawesi). Dalam rangka mengungkap permasalahan yang dialami oleh nelayan  lokal teluk Labuan Uki, metode yang digunakan dalam penelitian ini adalah metode survey, jenis studi kasus, dimana informasi dari responden (nelayan lokal teluk Labuan Uki)  dikumpulkan dengan menggunakan kuesioner, wawancara, pengamatan dan observasi.  Sedangkan metode analisis untuk menjawab tujuan penelitian yaitu analisis deskriptif. Adapun hasil yang ingin dicapai dalam penelitian ini adalah : 1) bagaimana  kondisi sosial ekonomi  nelayan lokal Teluk Labuan Uki, Kabupaten Bolaang Mongondow; 2) tingkat produktivitas alat dan peralatan tangkap yang digunakan, serta jenis ikan tangkapan; 3) Mengetahui sistem pemasaran dan pengolahan hasil tangkapan ikan nelayan lokal Teluk Labuan Uki, kabupaten Bolaang Mongondow.   Keyword : nelayan lokal, alat tangkap bagan, teluk labuan uki   ABSTRACT This study was done to investigate the socio-economical conditions of the local fishermen of Labuan Uki Bay , Bolaang Mongondow ; productivity of tools and fishing equipment used and the type of fish catches and marketing systems, and processing of the catch . The method used in this study was a survey method , type of case study , which collected information from respondents using questionnaires, interviews, and observation. Methods of analysis to answer the research purpose was descriptive analysis. The results showed that socio-economical conditions of local fishermen of  Labuan Uki Bay were still low , where the majority of their houses were huts and made of wood ; the largest percentage of low educational level of elementary and secondary school . Fishing gear used was “bagan” boat with a length of between 7-18 m, using an engine of 5.5 OD ( katinting ), and a net of 4.5 to 10 m depth, run by one person, usually done by the owner . The average of catching period was 12 hours per trip, starting from 18.00 PM until 06.00 AM, while the majority of fish caught were anchovy ( Stelopohorus spp ) . In peak season , the catch reaches 100 bowls ( 10 bowls = 1 kg ) per trip , while in the bad and  transitional season ranged from  5 to 40 bowls per trip. Anchovy catches were dried using sun heat and placed on a rack , processed products were then sold to middlemen ( tibo - tibo ) at a price of Rp 15,000 per kg . There were some processed through boiling and then dried with a tool , and sold at Rp . 35.000 per kg . Keyword : local fishermen , “bagan” fishing gear, Labuan Uki Bay
The Use Of Marine Sponge Crude Extract To Improve The Resistance Of Tilapia Fish (Oreochromis niloticus) To Streptococcus agalactiae Infections Shifa A. Schram; Reiny A. Tumbol; Reni L. Kreckhoff
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23723

Abstract

This study aims to examine the effect of the use of crude marine sponge extract on the resistance of streptococcus agalactiae infection in tilapia, Oreochromis niloticus, and to establish the effective dose of crude sponge extract in improving the immune system and the growth of the fish. The sponge used in the study was Cribrochalina sp. taken from Malalayang waters, Manado. The fish were taken from Freshwater Aquaculture Center, Tatelu. The fish were acclimatized for a week. After being acclimatized the fish were given feed added with sponge crude extract as a treatment with different concentrations of 20 g, 40 g and 60 g / Kg of feed for 14 days as much as 5% / body weight / day with the frequency of feeding twice a day at 10:00 am and at 5:00 p.m. After being treated, the fish was challenged with S. agalactiae. The data collected consisted of tilapia resistance, Total Leukocyte Count (TLC) as immune parameters and absolute growth. The results showed that the addition of crude extracts of Cribrochalina sp. into feed can increase TLC and growth of tilapia (p <0.05). The best results were achieved in fish fed with the addition of sponge crude extract of 40 g/kg feed. The survival rate of tilapia fed with treatment diet then challenged with pathogenic bacteria S. agalactiae showed the best results (100% survival rate) compared to controls (75%). In conclusion, feeding with a crude extract of Cribrochalina sp. has the potential to increase the immune system and growth of tilapia.Keywords: Crude Extract, Marine Sponges, Cribrochalina sp., Tilapia, Resistance, Streptococcus agalactiaeABSTRAKPenelitian ini bertujuan untuk mengkaji pengaruh penggunaan ekstrak kasar spons laut terhadap resistensi ikan nila dalam menghadapi serangan Streptococcus agalactiae, mengidentifikasi spons yang digunakan, serta mengukur pengaruh serta menetapkan dosis pemberian ekstrak kasar spons untuk meningkatkan sistem imun dan pengaruhnya terhadap pertumbuhan ikan nila. Spons yang digunakan dalam penelitian adalah spons Cribrochalina sp. yang diambil dari perairan Malalayang. Ikan uji diambil dari Balai Budidaya Air Tawar Tatelu, Provinsi Sulawesi Utara. Ikan diaklimatisasi selama seminggu. Setelah diaklimatisasi ikan diberi pakan yang ditambahkan dengan ekstrak kasar spons sebagai perlakuan dengan konsentrasi berbeda yaitu 0 g, 20 g, 40 g dan 60 g/kg pakan selama 14 hari sebanyak 5%/berat tubuh/hari dengan frekuensi pemberian pakan dua kali sehari yaitu jam 10.00 pagi dan jam 17.00 sore. Setelah diberi perlakuan, ikan diuji tantang dengan bakteri S. agalactiae. Data yang dikumpulkan terdiri dari kelangsunganhidup ikan nila. Total leukosit sebagai parameter imun dan pertumbuhan mutlak. Hasil penelitian mendapatkan bahwa penambahan ekstrak kasar spons Cribrochalina sp. ke dalam pakan mampu meningkatkan total leukosit dan pertumbuhan ikan nila (p<0.05). Dimana hasil terbaik dicapai pada ikan yang diberi pakan dengan penambahan ekstrak kasar spons sebanyak 40 g/kg pakan. Kelangsungan hidup ikan nila yang diberi pakan perlakuan yang diuji tantang dengan bakteri patogen menunjukkan hasil yang paling baik (tingkat kelangsungan hidup 100%) dibandingkan dengan kontrol (75%). Sebagai kesimpulan bahwa pemberian pakan dengan ekstrak kasar spons Cribrochalina sp. berpotensi untuk meningkatkan sistem kekebalan tubuh dan pertumbuhan pada ikan nila.Kata Kunci: Ekstrak kasar, Spons laut, Cribrochalina sp., Tilapia, Resistensi, Streptococcus agalactiae
Community Structure of Gastropod in Seagrass on Intertidal Area in The Tongkeina Village of Manado City Gladys L Saripantung; Jan FWS Tamanampo; Gaspar Manu
Jurnal Ilmiah PLATAX Vol. 1 No. 3 (2013): EDISI MEY - AGUSTUS 2013
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.1.3.2013.2567

Abstract

ABSTRACT Gastropod community is one of the important components of the food chain in the seagrass beds. The purpose of this study is to identify the types of gastropods and to determine population density, relative population density, species diversity, dominance and species associations of gastropod community at three study sites in Tongkeina, Manado city. The study sites were Bahowo, Batu Meja and Rap-Rap. Data were collected by sampling methods using quadratic transect technique. The study was conducted from November to December 2012. Over all, 20 species of gastropods comprising 7 families in 4 orders were collected. Among all sites, the highest density of 35,64 individuals/m2 was found in Bahowo site and the lowest density of 14,84 individuals/m2 was found in Rap-Rap site. The highest relative density was found at Batu Meja site, shown by Columbella versicolor species with relative density of 55.9%. Diversity index ranged from 1,4786 (Batu Meja) – 1,9382 (Rap-Rap). Batu Meja site shown an index value of C = 0,35 which indicating that there is dominance by 2 species, i.e.Columbella vesicolor and Columbella rusticoides, in that site.Associations between gastropod species at all three locations formed either positive or zero association, whereas negative associations between gastropod populations were not found. Keywords : community structure, gastropods, seagrass, Tongkeina, Manado  ABSTRAK Komunitas gastropoda merupakan salah satu komponen penting dalam rantai makanan di padang lamun.  Tujuan penelitian ini adalah mengidentifikasi jenis gastropoda dan mengetahui kepadatan populasi, kepadatan relative populasi, keanekaragaman spesies, dominasi dan asosiasi antar spesies gastropoda yang telah dilakukan pada tiga lokasi penelitian di kelurahan Tongkeina kota Manado. Pengambilan data dilakukan dengan metoda sampling yang menggunakan teknik transek kuadrat. Waktu penelitian pada bulan November sampai Desember 2012.  Berdasarkan hasil penelitian, diperoleh 20 spesies gastropoda yang terdiri dari 7 famili dalam 4 ordo. Kepadatan tertinggi dari semua stasiun adalah Stasiun Bahowo yakni 35,64 ind/m2, Stasiun Rap-Rap adalah stasiun yang memiliki kepadatan terendah dari ketiga stasiun penelitian yakni 14,84 ind/m2. Kepadatan relative tertinggi terdapat di Stasiun 1 (Batu Meja) yaitu pada spesies Columbella versicolor dengan kepadatan relatif  55,9 %. Indeks keanekaragaman berkisar 1,4786 (Batu Meja)–1,9382 (Rap-Rap). Stasiun Batu Meja dengan nilai indeks C=0,35 menunjukan 2 spesies yang mendominasi yakni Columbella vesicolor dan Columbella rusticoides. Asosiasi antara spesies gastropoda di ketiga lokasi membentuk asosiasi positif dan asosiasi nol, sedangkan asosiasi negative antara populasi gastropoda tidak ditemukan. Kata kunci : struktur komunitas, gastropoda, lamun, Tongkeina, Manado
DNA Isolation And Amplification of the rbcL (ribulose-1,5- bisphosphate carboxylase/oxygenase large subunit) gene of Caulerpa sp., Gracilaria sp., And Sargassum sp. Biondi Tampanguma; Grevo S. Gerung; Veibe Warouw; Billy Th Wagey; Stenly Wulllur; Deiske A. Sumilat; Hens Onibala
Jurnal Ilmiah PLATAX Vol. 8 No. 2 (2020): ISSUE JULY-DECEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.8.2.2020.30003

Abstract

DNA isolation and gene amplification of algae are significantly influenced by various factors such as characteristics and components of the algae cell wall. Therefore techniques and methods of DNA isolation in certain algae, sometimes only succeed in one particular species and can not be applied to another algae species. Based on that issue, this study was conducted with the aims to determine the succeed of DNA isolation and amplify the rbcL gene as a target gene for identification. Algae DNA was isolated by using innuPrep Plant DNA commercial kit, and the second one with a modified conventional Cetyl Trimetyl Ammonium Bromide (CTAB) method,  for the amplification process was using rbcL gene (ribulose-1,5-bisphosphate carboxylase / oxygenase large subunit) with two pairs of primers : rbcL 7F-753R and rbcL 577F-rbcSR. The results showed that the DNA of Gracilaria sp was succeed isolated by using CTAB method and it was denoted by the presence of DNA bands in agarose gel. Meanwhile DNA amplification for Gracilaria sp., and Sargassum sp., were succeed amplified with the appearance of DNA bands. But in algae Caulerpa sp., was only succeed on 1 pair of primary rbcL 7F and 7.Keywords : DNA, gene rbcL, algae Caulerpa sp., Sargassum sp., Gracilaria sp;AbstrakIsolasi DNA dan amplifikasi gen pada alga sangat dipengaruhi oleh beberapa faktor seperti karakter dan komponen pada dinding sel alga. Oleh karena itu proses isolasi DNA terkadang bisa berhasil pada satu jenis alga, namun tidak berhasil pada jenis alga lainnya. Oleh karena alasan tersebut, maka penelitian ini dilakukan untuk menentukan keberhasilan Isolasi DNA dan mengamplifikasi gen rbcL sebagai gen target identifikasi. Penelitian ini dilakukan dengan tahapan awal Isolasi DNA yang menggunakan kit komersil innuPrep Plant DNA Kit, dan metode konvensional Cetyl Trimetyl Ammonium Bromide (CTAB) yang telah dimodifikasi. Sedangkan untuk proses amplifikasi, menggunakan gen rbcL (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit) digunakan dua pasang primer yaitu rbcL 7F-753R dan rbcL 577F-rbcSR. Hasil isolasi DNA dari alga Gracilaria sp berhasil diisolasi menggunakan metode CTAB yang ditandai dengan adanya pita DNA pada gel agarose. Amplifikasi DNA pada alga Gracilaria sp., dan Sargassum sp., berhasil diamplifikasi dengan munculnya pita DNA. Namun pada alga Caulerpa sp. hanya berhasil pada 1 pasang primer rbcL 7F dan753R.Kata kunci : DNA, gen rbcL, alga Caulerpa sp., Sargassum sp., Gracilaria sp.
Lactococcus garvieae Isolates from Rainbow Trout (Oncorhynchus mykiss, W.) Compared by PLG and SA1B10 PCR Primer Pairs Altan, Erbülent; Korun, Jale
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.32561

Abstract

The aim of this study was to identify and compare Lactococcus garvieae strains using the PLG primer pair and SA1B10 primer pair. Also, the antibiotic resistance of the strains was investigated in the study. For this aim, commercial trout farms at Kemer, Korkuteli, and Manavgat, Antalya Province, Turkey were visited every month from June to September 2018. Thirty sick fish were sampled in the study. Lethargy, anorexia, darkening of skin color, unilateral or bilateral exophthalmos, opacification and hemorrhages in the eyes of the sick fish, and abdominal dropsy were observed. At necropsy, hemorrhages in the internal organs, splenomegaly, and darkening of the spleen and ascites were present. Seventy-five strains from sick fish samples verified on the basis of the biochemical characterization and PCR studies. The strains showed homogeneity in terms of phenotypic characteristics and all strains were identified as Lactococcus garvieae. The PCR technique was applied to 50 of 75 strains using PLG primer pair. While 47 of 50 strains gave positive results, amplification was not observed in 3 strains. When the PCR technique using SA1B10 primer pair was applied to a total of 25 strains, 3 of which were negative, 22 of which gave positive amplification, and 25 strains gave positive results. Resistance was determined by the disk diffusion method. All seventy-five strains were resistant to ampicillin and all strains were susceptible to bacitracin and tetracycline. It was found that the resistance and susceptibility of the strains showed variation to other antibiotics used in the study.Keywords: Lactococcus garvieae; Rainbow Trout; PCR; Antibiotic
Heavy Metal (Ni, Fe) Concentration in Water and Histopathological of Marine Fish in the Obi Island, Indonesia. Muhammad Aris; Tamrin Tamrin
Jurnal Ilmiah PLATAX Vol. 8 No. 2 (2020): ISSUE JULY-DECEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.8.2.2020.30673

Abstract

This study aims to determine the content of nickel (Ni) and iron (Fe) as well as histopathological analysis of marine fish in Obi Island waters as a bioindicator of pollution. Besides, water quality conditions were carried out in-situ and ex-situ observations. The parameters observed were temperature, brightness, salinity, pH, dissolved oxygen, nitrate, orthophosphate, ammonia, iron (Fe), and nickel (Ni). The results showed the temperature range between 26.48 ℃ to 27.99 ℃ below the quality standard or low temperature. The brightness of the relationship between 12 m and 13 m is under quality standards. The salinity range between 31.01 ppt to 32.13 ppt below the quality standard. The pH range is from 8.6 to 8.7 in high or alkaline conditions. Ammonia range between 0.4 mg / L to 0.7 mg / L exceeds the quality standard. The range of nitrate between 0.009 mg / L to 0.012 mg / L exceeds the quality standard. The range of phosphate between 0.016 mg / L to 0.019 mg / L exceeds the quality standard. The DO range between 3.68 mg / L to 3.77 mg / L lower than the quality standard. The metal range of 0.6 mg / L to 0.9 mg / L exceeds the quality standard. The range of Ni metal between 0.06 mg / L to 0.09 mg / L exceeds the quality standard. Histopathological analysis showed that the liver had a hemorrhage, degeneration of blood vessels, vacuolate degeneration, necrosis, or cell death. The muscles experience edema, degeneration of muscle fibers, atrophy of muscle bundles, vacuolar degeneration of muscle Bundles, hemorrhage, infiltration of lymphocytes, and necrosis. The intestine experience infiltration of lymphocytes, melanomacrophages, and necrosis. While P. tayenus fish ovaries showed necrosis structure oocytes. This research can be a reference for warning of heavy metal pollution in Obi Island waters, binding to the nature of heavy metals that can accumulate in fish tissue.Keywords: Water quality; Heavy Metal; Pollution; Histopathological; Obi Island.
Mangrove Community Structure Morowali District, Central Sulawesi Province, Case In Maratape Village, Lafeu Village, And Labota Village Joshian Nicolas William Schaduw
Jurnal Ilmiah PLATAX Vol. 8 No. 2 (2020): ISSUE JULY-DECEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.8.2.2020.31538

Abstract

The research purpose aims at analyzing the structure of the existing mangrove communities along the coastal area of Morowali District, Central Sulawesi Province. The sampling was taken from three villages, i.e. Maratape Village, Lafeu Village, and Labota Village. This research used the quadrant line transect method. The analysis and description were made after the method had been conducted. The composition of mangrove species found in this research was 3 (three) types, i.e. Rhizophora apiculata, Sonneratia alba, and Rhizophora stylosa. Of the three species the research finds, the dominant ones are Rhizophora apiculata and Sonneartia alba. The highest species density values were found at Station 1 (M01), i.e. R. apiculata. The frequency value had the highest value found at Station 2 (M02), i.e. R. apiculata type, and the highest relative frequency was R. apiculata. The highest closing value, i.e. S. albater type could be found at Station 2 (M02). The importance value index of Rhizophora apiculata type at the Station 1 (M01) had the highest value and followed in Station 3 (M03), i.e. Sonneratiaalba type, the lowest INP analysis in all stations was Rhizophora type stylosa. The dominant mangrove in 3 (three) Morowali District Stations were Rhizophora apiculata and Silneratia alba types. Based on the research results conducted on the structure of mangrove ecosystem communities in Morowali District, Central Sulawesi Province (Maratape Village Menui Kepulauan Sub-District (station 1), Lafeu Village Bungku Pesisir Sub-District (station 2) and Labota Village Bungku Tengah Sub-District (station 3) are definitely classified as natural and have not experienced significant degradationKeywords: Community Structure, Mangrove, Morowali, Central SulawesiAbstrakKajian ini bertujuan untuk menganalisis struktur komunitas mangrove yang ada disepanjang pesisir Kabupaten Morowali Provinsi Sulawesi Tengah. Sampling dilakukan pada tiga desa yaitu Desa Maratape, Desa Lafeu, dan Desa Labota. Kajian ini menggunakan metode line transek kuadaran dilanjutkan dengan analisissnya, kemudian dideskripsikan. Komposisi jenis mangrove yang ditemukan sebanyak 3 (tiga) jenis yaitu Rhizophora apiculata, Sonneratia alba, dan Rhizophora stylosa. Dari ketiga jenis yang ditemukan yang dominan yaitu Rhizophora apiculata dan Sonneartia alba. Nilai kerapatan jenis tertinggi terdapat di Stasiun 1 (M01) yaitu R. apiculata yaitu. Nilai frekuensi memiliki nilai tertinggi terdapat di Stasiun 2 (M02) yaitu jenis R. apiculata, dan frekuensi relatif tertinggi R. apiculata. Nilai penutupan tertinggi yaitu jenis S. alba terdapat di Stasiun 2 (M02) Indeks nilai penting jenis Rhizophora apiculata di Stasiun 1 (M01) memiliki nilai tertinggi dan di ikuti Stasiun 3 (M03) jenis Sonneratia alba, analisis INP yang terendah dari semua stasiun yaitu jenis Rhizophora stylosa. Mangrove dominan di 3 (tiga) stasiun Kabupaten Morowali  adalah dari jenis  Rhizophora apiculata dan Sonneratia alba. Berdasarkan hasil penelitian yang telah dilakukan terhadap struktur komunitas ekosistem mangrove di Kabupaten Morowali Provinsi Sulawesi Tengah (Desa Maratape Kecamatan Menui Kepulauan (stasiun 1), Desa Lafeu Kecamatan Bungku Pesisir (stasiun 2) dan Desa Labota Kecamatan Bungku Tengah (stasiun 3)  masih tergolong alami dan belum mengalami degradasi yang berarti.Kata kunci : Struktur komunitas; Mangrove; Morowali; Sulawesi Tengah
PCR-RFLP Design for Authentication of Red Snapper Species Based on CYB Gene Timbuleng, Kevin; Kolondam, Beivy J; Katili, Deidy
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.33541

Abstract

The PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technique is a species identification method that can facilitate food inspection agencies to overcome mislabelling. In addition, this technique is simple, fast, powerful, and cheap compared to DNA barcodes. This study aimed to accommodate the problem in determining restriction endonuclease enzymes for the digestion of PCR end products through the design of snapper species authentication using the CYB gene in silico. Differentiation of CYB gene sequences with DNA barcoding showed that all species could be differentiated with the highest similarity level in Red Fish (Sebastes) species by 98.9% and snapper species between L. malabaricus and L. erythropterus by 99.8%. Enzyme tracing based on sequences variation of snapper species with substitution species found three potential enzymes from the CYB gene sequence, namely, Accl, Fnu4HI, and Tsp45I. Where all these enzymes can discriminate red snapper species among other.Key words: PCR-RFLP; CYB gene; Red snapperAbstrakTeknik PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) merupakan metode identifikasi spesies yang dapat memfasilitasi lembaga inspeksi makanan untuk mengatasi mislabelling. Selain itu, teknik ini sederhana, cepat, kuat, dan murah dibandingkan dengan barcode DNA. Penelitian ini bertujuan untuk mengakomodasi masalah dalam menentukan enzim restriksi endonuklease untuk digesti produk akhir PCR lewat perancangan autentikasi spesies ikan kakap menggunakan gen CYB secara in silico. Diferensiasi sekuens gen CYB dengan DNA barcoding menunjukkan semua spesies dapat dibedakan dengan tingkat kesamaan tertinggi pada spesies Red Fish (Sebastes) sebesar 98,9% dan spesies kakap antara L. malabaricus dan L. erythropterus sebesar 99,8%. Penelusuran enzim berdasarkan variasi sekuens spesies kakap dengan spesies substitusi ditemukan sebanyak tiga enzim yang berpotensi yaitu, Accl, Fnu4HI, dan Tsp45I. Semua enzim tersebut dapat memdiskriminasikan spesies kakap merah dari spesies lainnya.Kata kunci: PCR-RFLP; gen CYB; ikan kakap merah
Health Condition of Tridacna sp. in the waters of Obi Island, Indonesia Tamrin Tamrin; Muhammad Aris
Jurnal Ilmiah PLATAX Vol. 8 No. 2 (2020): ISSUE JULY-DECEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.8.2.2020.30672

Abstract

Tridacna sp. is one of the protected heritage in Indonesia, because its population has declined dramatically. Tridacna sp. is the largest type of shellfish in water. Like a clam, Tridacna sp. can be used as a bioindicator of the aquatic environment because it can accumulate more heavy metals than other aquatic organisms due to its nature as a filter feeder. This study aims to determine the health condition of Tridacna sp. in the waters of Obi Island with a histopathological analysis approach. The histopathological analysis aims to see the level of tissue damage due to the accumulation of heavy metals. Observation of water quality was also observed in this study. Water quality parameters observed in-situ are temperature, brightness, salinity, pH, and dissolved oxygen. While the water quality parameters observed ex-situ are nitrate, orthophosphate, ammonia, iron (Fe), and nickel (Ni). Observations show that the water temperature is at 27.99 ℃. Water brightness is at 13 m. Salinity is at 32.13 mg / l. The acidity of the waters is 8.64. Ammonia is at 0.4 mg / L. Nitrate is at 0.009 mg / L. Orthopedics are at 0.016 mg / L. Dissolved oxygen waters are at 3.77 mg / L. The iron (Fe) level of water is at 0.6 mg / L. The level of nickel (Ni) waters is at 0.06 mg / L. This study shows the health condition of Tridacna sp. the histological approach shows that the condition of the network has changed. Symptoms of this change indicate the condition of the Tridacna sp. degeneration and cell necrosis. This change is thought to be influenced by heavy metals. Heavy metal content in liquids exceeds the quality standard threshold.Keywords:  Tridacna sp.; Heavy metal; Water quality; histopathological.
Community Structure of Zooplankton in the waters of Kampung Ambong Likupang, North Minahasa Rizki Antuke; Gaspar D. Manu; Rose O. S. E. Mantiri; Fransine B. Manginsela; Adnan S. Wantasen; Deiske A. Sumilat
Jurnal Ilmiah PLATAX Vol. 8 No. 2 (2020): ISSUE JULY-DECEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.8.2.2020.31629

Abstract

Zooplankton has an important role as a linking chain between primary producers and biota at higher trophic levels. This organism is a component in the food chain that has a dual role as both the first consumer and the second consumer. They are intermediaries between plankton and nekton groups. This research was conducted at the field station of Fisheries and Marine Sciences Faculty of Sam Ratulangi University, which is located in Kampung Ambong, Likupang, North Minahasa Regency, to identify the species and to determine the structure of the Zooplankton community through: Density, Relative Density, Diversity (H '), Dominance (e ), and Uniformity (C). Based on the results of species identification, 20 species from 15 genera in 3 classes were obtained, namely Hexanauplia (13 genus 18 species), Crustacea (1 genus 1 species), and Malacostraca (1 genus 1 species). The results of the community structure analysis obtained moderate uniformity values at points 1, 2, and 3. The dominance index shows low values at all three points, where point 2 C = 0.17 and at points 1 and 3 C = 0.20 This means that there is no types of plankton that dominate the area. Temperature 28 ° C, Salinity 300/00, and pH = 7 indicate conditions that are still in a good range as a place to live and grow Zooplankton.Keywords: Zooplankton; Diversity; Community Structure; LikupangAbstrakZooplankton mempunyai peran penting sebagai rantai penghubung produser primer dengan biota pada tingkat trofik yang lebih tinggi, juga merupakan salah satu komponen dalam rantai makanan yang berperan ganda baik sebagai konsumen tingkat pertama maupun konsumen tingkat ke dua, selanjutnya merupakan penghubung antara plankton dan nekton. Penelitian ini dilakukan di Laboratorium Basah Fakultas Perikanan dan Ilmu Kelautan Universitas Sam Ratulangi, Kampung Ambong, Likupang, Kabupaten Minahasa Utara, untuk mengetahui jenis dan struktur komunitas Zooplankton melalui: Kepadatan, Kepadatan Relatif, Keanekaragaman (H’), Dominansi (e), dan Keseragaman (C). Berdasarkan hasil Identifikasi jenis, diperoleh 20 spesies dari 15 genus dalam 3 klas, yaitu Hexanauplia (13 genus 18 spesies), Crustacea (1 genus 1 spesies), dan Malacostraca (1 genus 1 spesies).  Hasil analisis struktur komunitas diperoleh nilai keseragaman yang sedang pada titik 1, 2, dan 3. Indeks Dominansi menunjukkan nilai yang rendah  pada ketiga titik, dimana titik 2  C = 0,17 dan pada titik 1 dan 3 C = 0,20  Artinya tidak terdapat jenis plankton yang mendominsi daerah tersebut. Suhu 28°C, Salinitas 300/00, dan pH= 7  menunjukkan kondisi yang masih dalam kisaran baik sebagai tempat hidup dan bertumbuhnya Zooplankton.Kata kunci: Zooplankton; Keanekaragaman; Struktur Komunitas; Likupang

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