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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 542 Documents
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Front Cover JBBI Vol 6, No 1, June 2019 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (885.549 KB) | DOI: 10.29122/jbbi.v6i1.3704

Abstract

Front Cover JBBI Vol 1, No 1, December 2014 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 20140
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (905.939 KB) | DOI: 10.29122/jbbi.v1i1.1038

Abstract

Back Cover JBBI Vol 4, No 1, June 2017 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 1 (2017): June 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.547 KB) | DOI: 10.29122/jbbi.v4i1.2252

Abstract

EVALUASI AKTIVITAS INHIBISI XANTIN OKSIDASE DAN KANDUNGAN SENYAWA POLIFENOL DARI EKSTRAK SAPPAN Ningsih, Sri; Churiyah, .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2349.438 KB) | DOI: 10.29122/jbbi.v5i2.2880

Abstract

Evaluation of xanthine oxidase inhibitory activity and polyphenolic content of sappan extractABSTRACTHyperuricemia is a disease that is characterized by a high uric acid level, in which the number of patients tends to increase every year. This research was intended to evaluate the xanthine oxidase (XO) inhibitory activity and determinate the total polyphenol content of heartwood sappan (Caesalpinia sappan L.) extract. The extract was prepared by macerating the dry powder wood using 70% ethanol at room temperature. The quality of ethanolic extract obtained was evaluated based on BPOM guideline. XO inhibitory power was determined by measuring uric acid produced in the xanthine/XO system in vitro. The polyphenol content of the extract was measured using Folin-Ciocalteu reagent spectrophotometrically. The results showed that the quality of sappan semisolid extract fulfilled the required standard. Sappan extract inhibited XO activity by 98% relative to the positive control, allopurinol, at the final extract concentration of 100 µg/mL. The total polyphenol content was 26% of the crude extract. It could be concluded that sappan ethanolic extract has the potential to be developed as an ingredient for hyperuricemia treatment.Keywords: hyperuricemia, sappan, total polyphenol, xanthine, xanthine oxidase ABSTRAKHiperurisemia adalah penyakit yang dicirikan dengan kadar asam urat tinggi dimana prevalensi penderita cenderung meningkat. Penelitian ini bertujuan untuk mempelajari aktivitas esktrak kayu sappan (Caesalpinia sappan L.) dalam menginhibisi enzim XO (xantin oksidase) secara in vitro dan penentuan kadar senyawa polifenol total yang terkandung di dalamnya. Ekstrak dibuat dengan cara maserasi serbuk kayu menggunakan pelarut etanol 70% pada suhu kamar. Kualitas ekstrak dievaluasi mengacu pada parameter karakterisasi ekstrak yang ditetapkan oleh BPOM. Aktivitas inhibisi XO ditetapkan dengan mengukur kadar asam urat yang terbentuk pada sistem xantin/XO in vitro. Kadar polifenol ekstrak diukur menggunakan pereaksi Folin-Ciocalteu secara spektrofotometri. Hasil analisis menyatakan bahwa kualitas ekstrak kental sappan memenuhi persyaratan yang ada. Pengujian inhibisi XO dengan pembanding positif allopurinol pada konsentrasi akhir ekstrak sebesar 100 µg/mL menunjukkan bahwa ekstrak mempunyai kekuatan menginhibisi XO sebesar 98% relatif terhadap pembanding positif allopurinol. Kadar senyawa polifenol total dalam ekstrak sappan sebesar 26% dari ekstrak kasar. Dari penelitian ini dapat disimpulkan bahwa ekstrak sappan mempunyai potensi untuk dikembangkan sebagai bahan untuk mengatasi hiperurisemia.Kata Kunci: hiperurisemia, polifenol total, sappan, xantin, xantin oksidase
EKSTRAK KAYU TEGERAN (Cudrania javanensis Trécul) SEBAGAI ANTI JAMUR Peniophora sp. Darsih, Cici; Ilyas, Muhammad; Rosyida, Vita Taufika; Pratiwi, Diah; Indrianingsih, A. Wheni; Hernawan, .; Apriyana, Wuri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.684 KB) | DOI: 10.29122/jbbi.v6i1.3168

Abstract

Antifungal Activity of Tegeran Wood (Cudrania javanensis Trécul) Extracts against Fungus Peniophora sp.  ABSTRACTThe fungus Peniophora sp. can degrade natural dyes, causing discoloration on batik cloth. This study was aimed to evaluate the antifungal activity of tegeran wood (Cudrania javanensis) extracts against Peniophora sp. isolated from the aqueous extract of mahogany (Swietenia mahagoni) bark. Aqueous and methanol extraction procedures gave tegeran wood extracts whose concentrations were then varied into 100, 250 and 500 ppm. Phytochemical analysis of the extracts were carried out, and the results showed that the average values of the total polysaccharides and terpenoids of both aqueous and methanol extracts did not differ significantly (p<0.05), whereas those of polyphenols did (p<0.05). The total polyphenols of the methanol extract (484.723 mg GAE/g) was higher than that of the aqueous extract (389.903 mg GAE/g). Results showed that the methanol extract of tegeran wood showed higher antifungal activity against Peniophora sp. than the aqueous extract, and was also higher than ketoconazole control (100 ppm). The minimum inhibitory concentration of the methanol extract against Peniophora sp. growth was of 500 ppm with the antifungal activity value (AFA) of 76.30%.Keywords: antifungal activity, Cudrania javanensis, Peniophora sp., Swietenia mahagoni, wood extract ABSTRAKJamur Peniophora sp. dapat mendegradasi zat warna alam sehingga warna yang dihasilkan pada kain batik lebih pudar. Penelitian ini dilakukan untuk mempelajari aktivitas anti jamur dari ekstrak kayu tegeran (Cudrania javanensis) terhadap Peniophora sp. yang diisolasi dari ekstrak air kulit mahoni (Swietenia mahagoni). Ekstraksi menggunakan metanol dan air, menghasilkan ekstrak kayu tegeran, yang kemudian konsentrasinya divariasi menjadi 100, 250 dan 500 ppm. Analisis fitokimia ekstrak kayu tegeran dilakukan, dan hasilnya menunjukkan bahwa nilai rata-rata total polisakarida dan terpenoid ekstrak metanol dan air kayu tegeran tidak berbeda nyata (p<0,05). Sedangkan total polifenol kedua ekstrak berbeda nyata (p<0,05). Total polifenol ekstrak metanol kayu tegeran (484,723 mg GAE/g) lebih besar dibandingkan dengan ekstrak air (389,903 mg GAE/g). Hasil menunjukkan bahwa aktivitas anti jamur ekstrak metanol lebih baik dibandingkan dengan ekstrak air kayu tegeran, dan juga lebih tinggi dibandingkan dengan kontrol ketoconazole (100 ppm). Konsentrasi minimum ekstrak metanol kayu tegeran yang dapat menghambat pertumbuhan jamur Peniophora sp. adalah 500 ppm dengan nilai penghambatan (AFA) sebesar 76,30%.Kata Kunci: aktivitas anti jamur, Cudrania javanensis, ekstrak kayu, Peniophora sp., Swietenia mahagoni
PRELIMINARY CYTOTOXIC EVALUATION OF Andrographis paniculata IN BREAST CANCER CELL LINES ., Tarwadi; ., Churiyah; Pongtuluran, Olivia Bunga; Juniarti, Fifit; Wijaya, Fery Azis
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 1 (2015): June 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (937.695 KB) | DOI: 10.29122/jbbi.v2i1.533

Abstract

Sambiloto (Andrographis paniculata) banyak digunakan untuk mengobati berbagai penyakit di Indonesia dan negara-negara Asia lainnya. Dalam studi ini, ekstrak metanol dan etanol sambiloto yang diperoleh dari B2PTO Tawangmangu telah diuji terhadap sel lini kanker payudara T47D dan MCF-7 dan sel lini normal fibroblast HFL-1 menggunakan reaksi enzimatik 3-(4,5-dimethylthiazoyl-2-yl) 2,5-diphenyltetrazoliumbromide (MTT). Uji in vitro terhadap sel lini normal fibroblast HFL-1 menunjukkan bahwa 50 ppm ekstrak metanol sambiloto tidak menghambat pertumbuhan sel. Tetapi, ekstrak metanol dan etanolnya menghasilkan IC50 yang relatif rendah pada sel lini kanker payudara, yaitu 111 ppm dan 122 ppm pada sel lini MCF-7 dan 70 ppm dan 197 ppm pada sel lini T47D. Selain itu, campuran ekstrak sambiloto yang mengandung 25% ekstrak Thyponium divaricatum dan Anredera cordifolia memberikan daya hambat pertumbuhan pada sel kanker payudara MCF-7 yang lebih besar, dengan nilai IC50 masing-masing adalah 68 ppm dan 34 ppm. Kesimpulannya, total ekstrak metanol atau etanol sambiloto yang diperoleh dari Tawangmangu memiliki potensi sebagai sumber senyawa anti-kanker serta perlu kajian lebih lanjut.Kata kunci: Ekstrak Andrographis paniculata, MTT, sel lini normal, sel lini kanker, aktivitas anti kanker ABSTRACTSambiloto (Andrographis paniculata) is widely used as medicine to treat various diseases in Indonesia and other Asian countries. In this study, methanolic and ethanolic extracts of sambiloto collected from B2PTO Tawangmangu have been tested againts breast cancer cell lines of T47D and MCF-7 and normal fibroblast cell line of HFL-1 using enzymatic reaction of 3-(4,5-dimethylthiazoyl-2-yl) 2,5-diphenyltetrazoliumbromide (MTT). In vitro assay performed on normal fibroblast of HFL-1 cell line showed that 50 ppm of methanolic extract of sambiloto did not inhibit cell growth. However, methanolic and ethanolic extracts of sambiloto gave relatively low of IC50 on breast cancer cell lines which were 111 ppm and 122 ppm on the MCF-7 cell lines and 70 ppm and 197 ppm on the T47D cell lines, respectively. In addition, the mixture of sambiloto extract containing 25% of Thyponium divaricatum and Anredera cordifolia extracts confered greater growth inhibition on breast cancer cell line of MCF-7, where IC50 values were 68 ppm and 34 ppm, respectively. In conclusion, the total methanolic or ethanolic extract of sambiloto collected from Tawangmangu has potency as a source of anti-cancer compounds and needs further study.Key words: Andrographis paniculata extract, MTT, normal cell line, cancer cell lines, anti-cancer activity
Appendix JBBI Vol 3, No 2, December 2016: Keyword Index and Author Index Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.522 KB) | DOI: 10.29122/jbbi.v3i2.1513

Abstract

Front Cover JBBI Vol 5, No 1, June 2018 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (777.736 KB) | DOI: 10.29122/jbbi.v5i1.2952

Abstract

MORFOLOGI SPORA DAN PERKEMBANGAN GAMETOFIT Davallia denticulata dan Davallia trichomanoides Meliza, Rezika; Chikmawati, Tatik; Sulistijorini, Sulistijorini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.519 KB) | DOI: 10.29122/jbbi.v6i1.2607

Abstract

Spore Morphology and Gametophyte Development of Davallia denticulata and Davallia trichomanoidesABSTRACTDavallia denticulata and D. trichomanoides are two attractive and decorative fern species for ornamental. Spore morphology has an important role in fern taxonomy, while media composition has important role in the growth and development of their gametophytes. Such information on the two fern species was lacking. Therefore, this study aimed to reveal the information of the spore morphology and gametophyte developmental stages of D. denticulata and D. trichomanoides on three different media. The spores were collected from Bogor, West Java. The spores were sown in three sterile media. Spore morphology and gametophyte development were observed under a stereoscopic microscope. Both gametophyte species reached their mature stage at 25 weeks after planting on the different media compositions. D. denticulata showed the best gametophyte development, and formed mature gametophytes on the media of vermiculite, sphagnum moss, and perlite, while D. trichomanoides grew best into maturity stage on the media containing vermiculite, and sphagnum moss. Thus, the presence of sphagnum moss in the media is an important material for the growth and development of Davallia gametophyte.Keywords: Davallia, development, gametophyte, growth, media  ABSTRAKDavallia denticulata dan D. trichomanoides merupakan dua spesies tumbuhan paku yang menarik dan indah untuk tanaman hias. Morfologi spora memiliki arti penting dalam taksonomi tumbuhan paku, sedangkan komposisi media berperan penting untuk pertumbuhan dan perkembangan gametofitnya. Informasi seputar hal ini terkait dua spesies tumbuhan paku tersebut belumlah ada. Penelitian ini bertujuan untuk mengungkap informasi mengenai ciri morfologi spora dan tahapan perkembangan gametofit D. denticulata dan D. trichomanoides pada tiga komposisi media berbeda. Pengambilan spora dilakukan di Bogor, Jawa Barat. Spora ditumbuhkan pada tiga media steril. Morfologi spora dan perkembangan gametofit diamati menggunakan mikroskop stereo. Kedua spesies memiliki waktu perkembangan terbaik untuk mencapai tahap gametofit dewasa yaitu 25 minggu pada komposisi media yang berbeda. D. denticulata berkembang dengan baik, dan membentuk gametofit dewasa pada media vermiculite, lumut sphagnum, dan perlite. D. trichomanoides berkembang hingga tahap gametofit dewasa dengan baik pada media vermiculite, dan lumut sphagnum. Dengan demikian keberadaan lumut sphagnum pada media sangat penting untuk pertumbuhan dan perkembangan gametofit Davallia.Kata Kunci: Davallia, gametofit, media, perkembangan, pertumbuhan
KAJIAN ELONGASI PADA TANAMAN IN VITRO GAHARU (Aquilaria beccariana van Tiegh) Fauzan, Yusuf Sigit Ahmad; Sandra, Edhi; Mulyono, Daru
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (737.168 KB) | DOI: 10.29122/jbbi.v2i2.511

Abstract

The population density of natural agarwood (Aquilaria beccariana) in Indonesia decreased to less than one tree per hectare. Efforts have been carried out on ex situ conservation of agarwood despite facing many obstacles. In vitro propagation is one alternative to speed up the recovery of natural agarwood populations. The purpose of this study was to obtain optimal elongation media for in vitro culture with addition of auxin and cytokinin, namely IBA, BAP and kinetin. The results showed that the best auxin-cytokinin combination was IBA 0.1 mg/L and BAP 0.05 mg/L. This combination increased the height and number of segments of A. beccariana with an average height of 1.64 cm and average number of sections of 6.40. It is suggested that this combination of IBA and BAP was the most effective compared to the other treatments. In addition, the combination of IBA 0 mg/L and BAP 0.03 mg/L gave rise to the best response to increase the number of shoots with an average of 1.91 shoots.Keywords: Aquilaria beccariana, shoot, elongation, auxin, cytokinin ABSTRAKKepadatan populasi gaharu (Aquilaria beccariana) alam di Indonesia kurang dari satu pohon per hektar. Upaya pelestarian gaharu ex situ telah banyak dilakukan tetapi masih banyak kendala. Perbanyakan gaharu in vitro merupakan salah satu cara alternatif untuk mempercepat pemulihan populasi gaharu alam. Tujuan penelitian ini adalah untuk memperoleh media elongasi yang optimal pada kultur in vitro gaharu dengan penambahan kombinasi zat pengatur tumbuh auksin dan sitokinin. Pada penelitian ini digunakan auksin IBA, serta sitokinin BAP dan Kinetin. Hasil penelitian elongasi diperoleh kombinasi auksin dan sitokinin terbaik yaitu, IBA 0,1 mg/L dan BAP 0,05 mg/L. Kombinasi ini meningkatkan tinggi dan jumlah ruas Aquilaria beccariana dengan tinggi rata-rata sebesar 1,64 cm dan jumlah ruas rata-rata sebesar 6,40 ruas. Pada kombinasi dan taraf ini diduga mekanisme kerja IBA dan BAP paling efektif dibanding perlakuan yang lain. Sedangkan kombinasi IBA 0 mg/L dan BAP 0,03 mg/L memberikan respon terbaik terhadap peningkatan jumlah tunas dengan rata-rata sebanyak 1,91 tunas.Kata Kunci: Aquilaria beccariana, tunas, elongasi, auksin, sitokinin

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