cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta pusat,
Dki jakarta
INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 542 Documents
 20   21   22   23   24 
ANALISIS FILOGENETIK BEBERAPA KLON KARET DENGAN MARKA AFLP (AMPLIFIED FRAGMENT LENGTH POLYMORPHISM) Suparningtyas, Juniza Firdha; Pramudyawardhani, Okky Dwi; Purwoko, Devit; Tajuddin, Teuku
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (940.1 KB) | DOI: 10.29122/jbbi.v5i1.2544

Abstract

Phylogenetic Analysis of Rubber Tree Clones using AFLP (Amplified Fragment Length Polymorphism) MarkerNational rubber productivity is lower than other rubber producing countries in the world. The DNA marker-based plant breeding program is required to increase latex production and other superior characters. Breeding process by identifying genetic diversity can be done using AFLP marker. The aim of this study was to analyze the phylogenetic six rubber clones. Pre-amplification and amplification process were performed using 64 primer pair combinations followed by electrophoresis in 6% polyacrylamide gel. A total of 2806 AFLP fragments have been detected. Phylogenetic tree of six clones showed 60% of genetic similarity, which was consisted of two groups. GT 1 clone in the first group and was separated from other clones in the second group. Sub-group at the phylogenetic peak contained IRR 104 and RRIM 600 clones with genetic similarity of 74%. The information obtained from this study showed the genetic diversity of the six rubber clones. The unique bands were obtained as marker specific which could be used to identify clones.Keywords: AFLP, DNA marker, phylogenetic tree, polymorphism, rubber clones ABSTRAKProduktivitas karet nasional masih lebih rendah dibanding dengan negara-negara penghasil karet lainnya di dunia. Diperlukan program pemuliaan tanaman karet yang berbasis marka DNA untuk meningkatkan produksi lateks serta karakter unggul lainnya. Proses pemuliaan untuk mengidentifikasi keragaman genetik bisa dilakukan dengan marka AFLP. Tujuan studi ini adalah untuk menganalisis filogenetik enam klon karet berdasarkan produktivitasnya. Proses pre-amplifikasi dan amplifikasi dilakukan dengan menggunakan 64 kombinasi pasangan primer yang dilanjutkan dengan analisis hasil elektroforesis pada gel poliakrilamid 6%. Sebanyak 2806 pita AFLP telah dihasilkan. Kekerabatan keenam klon menunjukkan kemiripan genetik sebesar 60%. Pohon filogenetik membentuk dua kelompok, yang memisahkan GT 1 dengan klon-klon lainnya. Sub-kelompok pada puncak filogenetik terdiri dari klon IRR 104 dan RRIM 600 dengan kemiripan genetik sebesar 74%. Informasi yang diperoleh telah menunjukkan keragaman genetik keenam klon karet yang bersifat polimorfis. Diperoleh pita-pita unik dari pasangan primer tertentu yang dapat berperan sebagai marka spesifik dan dapat digunakan untuk mengidentifikasi klon.Kata Kunci: AFLP, klon karet, marka DNA, pohon filogenetik, polimorfisme
KAJIAN PROSES PRODUKSI PUPUK HAYATI BIO-SRF DAN PENGUJIAN EFEKTIVITASNYA PADA TANAMAN BAWANG MERAH Sukmadi, R Bambang; Supriyo, Agus; Rupaedah, Bedah; Mira, Farida Rosana; Bakhtiar, Yenni; Ali, Asep; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 1 (2016): June 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.342 KB) | DOI: 10.29122/jbbi.v3i1.46

Abstract

This study aimed to assess the production process of biofertilizer Bio-SRF and determine its effectiveness on the growth and productivity of shallot plants. The Study of biofertilizer Bio-SRF production covering the cultivation of microbial cell biomass, granulation, and formulation of  biofertilizer products. Testing the effectiveness of biofertilizers on shallot plants using a randomized complete block design (RCBD) with five replicates, involving nine biofertilizers treatments and one control. The results showed that the population of cells on the granulated biofertilizer Bio-SRF was Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3.8 x 107 cfu/g, Burkholderia seminalis 7.4 x 108 cfu/g, Pseudomonas stutzeri 4.5 x 108 cfu/g and 60 mycorrhizal spores/g products. The effectiveness test showed that the biofertilizer treatments significantly affected plant height, the number of bulbs, weight of wet and dried bulbs produced. Application of biofertilizer Bio-SRF on shallot plants gave the best results of plant height 34.80 cm at harvest time, the number of bulbs per plant 4.78 bulb, weight of wet bulbs 3,81 kg/m2, weight of dried bulbs 3,27 kg/m2 and increased the yield of shallot production by 55.71% compared with no biofertilizer application.Keywords: Biofertilizer, Bio-SRF, production process, effectivity test, shallot plant ABSTRAKBio-SRF merupakan formula produk pupuk hayati yang mengandung campuran beberapa jenis mikroba penyubur tanah. Penelitian ini bertujuan untuk mengkaji proses produksi pupuk hayati Bio-SRF dan mengetahui efektivitasnya terhadap pertumbuhan dan produktivitas tanaman bawang merah. Kajian produksi pupuk hayati Bio-SRF meliputi perbanyakan biomassa sel mikroba, granulasi dan formulasi produk. Hasil penelitian menunjukkan bahwa populasi sel pada produk pupuk hayati Bio-SRF bentuk granul adalah Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3,8 x 107 cfu/g, Burkholderia seminalis 7,4 x 108 cfu/g, Pseudomonas stutzeri 4,5 x 108 cfu/g dan mikoriza 60 spora/g produk. Hasil uji efektivitas menunjukkan bahwa perlakuan pupuk hayati berpengaruh nyata terhadap tinggi tanaman, jumlah umbi, bobot basah dan bobot kering umbi bawang merah yang dihasilkan. Aplikasi pupuk hayati Bio-SRF pada tanaman bawang merah memberikan hasil terbaik yaitu dengan tinggi tanaman saat panen 34,80 cm, jumlah umbi per tanaman 4,78 umbi, berat basah umbi 3,81 kg/m2, berat kering umbi 3,27 kg/m2 dan dapat meningkatkan hasil produksi bawang merah sebesar 55,71% dibandingkan dengan tanpa aplikasi pupuk hayati.Kata kunci: Pupuk hayati, Bio-SRF, proses produksi, uji efektivitas, bawang merah
IDENTIFIKASI BAKTERI PATOGEN PENYEBAB PENYAKIT PURPLE SYNDROME PADA KARANG FUNGIA DI PULAU HARI SULAWESI TENGGARA Palupi, Ratna Diyah; Sadarun, Baru; Sawonua, Paiga Hanurin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1443.583 KB) | DOI: 10.29122/jbbi.v6i2.3116

Abstract

IDENTIFIKASI BAKTERI PATOGEN PENYEBAB PENYAKIT PURPLE SYNDROME PADA KARANG FUNGIA DI PULAU HARI SULAWESI TENGGARA Nowadays coral disease is one of the causes of damage to coral reefs in Indonesia. Causative agents were found for some types of coral disease. This study aims to identify the type of pathogenic bacteria that cause purple syndrome which attacks Fungia corals. The study was conducted using descriptive exploratory methods. Corals infected with purple syndrome were collected on Pulau Hari, Southeast Sulawesi, through scuba diving. Then, microbiological analysis was carried out which included isolation using the scatter method, purification using a scratch method, a challenge test (antagonistic), a Koch Postulate test, and DNA analysis of putative bacterial isolates. Results showed that 5 bacterial isolates lived in symbiosis with the corals infected with purple syndrome (PSMH1, PSMH2, PSMH3, PSMH4, and PSMH5). Based on the Koch postulate test, 2 bacterial isolates which were pathogenic were obtained, namely PSHM2 and PSHM4 isolates. These bacteria infected the test corals with the characteristics of coral skeleton damage and coral bleaching (dead). Based on biomolecular testing, the two isolates were members of Enterobacter cloacae with a 99% similarity level.Keywords: Coral disease; Enterobacter cloacae; Fungia coral; Hari island; Purple syndromeABSTRAKSaat ini penyakit karang menjadi salah satu penyebab kerusakan terumbu karang di Indonesia. Penyebab pembawa untuk beberapa jenis penyakit karang sudah ditemukan. Penelitian ini bertujuan untuk mengidentifikasi jenis bakteri patogen penyebab penyakit purple syndrome yang menyerang karang Fungia. Penelitian dilakukan menggunakan metode deskriptif eksploratif. Sampel karang yang terinfeksi purple syndrome diambil di Pulau Hari, Sulawesi Tenggara, melalui scuba diving. Selanjutnya, analisis mikrobiologi dilakukan yang meliputi isolasi menggunakan metode sebar, purifikasi menggunakan metode gores, uji tantang (antagonistik), uji Postulat Koch, dan analisa DNA isolat bakteri yang diduga bersifat patogen. Hasil penelitian menemukan 5 isolat bakteri yang bersimbiosis dengan karang yang terinfeksi penyakit purple syndrome (PSMH1, PSMH2, PSMH3, PSMH4, dan PSMH5). Berdasarkan uji postulat Koch, 2 isolat bakteri yang bersifat patogen didapatkan, yaitu isolat PSHM2 dan PSHM4. Bakteri tersebut menginfeksi karang uji dengan ciri kerusakan skeleton karang dan pemutihan karang (mati). Berdasarkan uji biomolekuler kedua isolat tersebut merupakan anggota Enterobacter cloacae dengan tingkat kemiripan 99%.
Back Cover JBBI Vol 2, No 2, December 2015 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.832 KB) | DOI: 10.29122/jbbi.v2i2.1055

Abstract

EFFECT OF NITROGEN SUPPLY IN CULTURE MEDIA AND LIGHT INTENSITY ON PHOTOSYNTHESIS OF Chlamydomonas reinhardtii Rupaedah, Bedah; Takahashi, Yuichiro
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 2 (2017): December 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (945.676 KB) | DOI: 10.29122/jbbi.v4i2.15

Abstract

Pengaruh Suplai Nitrogen pada Media Kultur dan Intensitas Cahaya Terhadap Proses Fotosintesis Chlamydomonas reinhardtiiOrganisms use nitrogen to produce, among others, amino acids, proteins, and nucleic acids. In this study, the effects of various concentrations of ammonium in culture media on the photosynthetic performance of Chlamydomonas reinhardtii were done under two light conditions: low and high intensity. The microbes were grown at low (75% NH4Cl dosage), normal (100% NH4Cl dosage, which was 2 M NH4Cl), and high (125% NH4Cl dosage) nitrogen content. Cells density and chlorophyll content were quantitatively determined. Immunoblotting technique was used to separate proteins based on molecular mass. In both low and high light intensity, cells grown in 75% NH4Cl dosage culture medium showed lower cell density and chlorophyll concentration than those grown in 100% and 125% NH4Cl dosage media. The later two media produced almost the same amount of cell density and chlorophyll concentration. In conclusion, 75% NH4Cl dosage was insufficient for C. reinhardtii cells to grow well. The results also showed that accumulation of photosystem I (PsaA and PsaD/F) and light harvesting complex II (LHCII) were higher in low light than in high light intensity.AbstrakOrganisme menggunakan nitrogen diantaranya untuk memproduksi asam amino, protein, dan asam nukleat. Dalam percobaan ini pengaruh berbagai konsentrasi amonium dalam media pada fotosintesis Chlamydomonas reinhardtii dilakukan di bawah dua kondisi cahaya: intensitas rendah dan tinggi. C. reinhardtii ditumbuhkan dalam medium dengan dosis nitrogen (N) rendah (75% dosis NH4Cl), normal (100% dosis NH4Cl, yakni NH4Cl 2 M), dan tinggi (125% dosis NH4Cl). Parameter yang diukur adalah  kepadatan sel dan konsentrasi klorofil. Analisis protein dilakukan dengan imunobloting untuk memisahkan protein berdasarkan massa molekul. Pada intensitas cahaya rendah dan tinggi, sel-sel pada medium dengan 75% NH4Cl menunjukkan kepadatan sel dan konsentrasi klorofil lebih rendah dibandingkan dengan 100% NH4Cl dan 125% NH4Cl, di mana kedua media ini menghasilkan kepadatan sel maupun konsentrasi klorofil yang hampir sama. Dengan demikian dapat disimpulkan bahwa 75% NH4Cl tidak cukup bagi C. reinhardtii untuk tumbuh dengan baik. Selain itu, akumulasi fotosistem I (PsaA dan PsaD/F) dan kompleks pemanenan cahaya II (LHCII) lebih tinggi pada sistem fotosintesis dengan intensitas cahaya rendah dibandingkan cahaya tinggi.Kata kunci: Chlamydomonas reinhardtii, fotosintesis, intensitas cahaya, klorofil, nitrogen
PENGARUH PEMBERIAN MANUR BROILER DENGAN FERMENTASI Lactobacillus casei TERHADAP KONVERSI PAKAN AYAM KAMPUNG Nururrozi, Alfarisa; Indarjulianto, Soedarmanto; Ramandani, Dhasia; Yanuartono, Yanuartono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (995.536 KB) | DOI: 10.29122/jbbi.v5i2.2774

Abstract

The Effect of Broiler Manure with Lactobacillus casei Fermentation on the Kampung Chicken Feed Convertion Ratio ABSTRACTHusbandry of kampung chicken is constrained by high feed prices and poor productivity. This study aims to utilize alternative feed materials derived from broiler manure to obtain a cheaper feed with good quality. Manure contains high nutrients. Manure was fermented using Lactobacillus casei to improve feed conversion. Two hundred chickens were divided into 4 groups (n = 50). Groups P1, P2, and P3 were given 4%, 8%, and 12% fermentation of L. casei, respectively. Group P0 was given a regular feed without L. casei. Each treatment group consisted of four replicates and were maintained for 60 days. The research design used was Completely Randomized Design subjected to analysis of variant (ANOVA) followed by Duncan test. The feed conversion values of groups P0, P1, P2, and P3 were 4.46; 4.38; 4.21; and 4.54, respectively. The results showed that the feed conversion was not significant in all groups. It was concluded that L. casei fermenter could not improve the feed conversion ratio (FCR).Keywords: FCR, fermentation, kampung chicken, Lactobacillus casei, manure ABSTRAKBudidaya ayam kampung terkendala tingginya harga pakan dan rendahnya produktivitas. Penelitian ini bertujuan memanfaatkan pakan alternatif bersumber manur (limbah kotoran) ayam broiler untuk memperoleh pakan murah dengan kualitas baik. Manur broiler masih mengandung nutrisi yang tinggi. Manur difermentasi menggunakan Lactobacillus casei untuk memperbaiki konversi pakan. Dua ratus ekor ayam dibagi menjadi 4 kelompok (n=50 ekor). Kelompok P1, P2, dan P3 masing-masing diberi ransum yang ditambah fermentasi L. casei sebanyak 4%, 8%, dan 12%. Kelompok P0 diberikan pakan biasa tanpa penambahan L. casei. Setiap kelompok perlakuan terdiri dari empat ulangan dipelihara selama 60 hari. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan analisis ragam yang dilanjutkan dengan uji Duncan. Konversi pakan dari kelompok P0, P1, P2, dan P3 berturut-turut 4,46; 4,38; 4,21; dan 4,54.  Hasil penelitian menunjukkan konversi pakan tidak berbeda nyata pada semua kelompok perlakuan. Dari hasil penelitian disimpulkan penggunaan fermenter L.casei pada pakan belum mampu memperbaiki konversi pakan.Kata Kunci: ayam kampung, FCR, fermentasi, Lactobacillus casei, manur
PERBANDINGAN TIGA KIT EKSTRAKSI RNA UNTUK ANALISIS TRANSKRIPTOMIKA PADA KELAPA SAWIT (Elaeis guineensis Jacq.) Zulaeha, Siti; Purwoko, Devit; Cartealy, Imam; Tajuddin, Teuku; Karyanti, .; Khairiyah, Hayat
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.283 KB) | DOI: 10.29122/jbbi.v6i1.3372

Abstract

Comparison of Three RNA Extraction Kits for Transcriptome Analysis of Oil Palm (Elaeis guineensis Jacq.) ABSTRACTObtaining high-quality RNA is very important at an early stage of molecular biology research. To isolate RNA, high skill and caution are required in following laboratory procedures because RNA is easily degraded, especially samples from plant tissue culture. One of the parameters used to check the total RNA quality is RIN (RNA Integrity Number). The aim of this study was to obtain RNA extraction methods on oil palm leaves, callus and somatic embryos that were of good quality and high concentrations for transcriptomic analysis. RNA extraction was carried out using Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) and RibospinTM Plant (Geneall) kit methods. The results showed that oil palm leaf, callus and somatic embryo RNA were successfully extracted using the RibospinTM (Geneall) kit. Based on the total RNA number of more than 4 μg and the RIN value of more than 7, the extracted RNA could be used in RNA sequencing for transcriptomic analysis. Keywords: callus, oil palm, RNA analysis, RNA quality, somatic embryo ABSTRAKMenghasilkan RNA berkualitas tinggi sangatlah penting pada tahap awal penelitian biologi molekuler. Untuk mengisolasi RNA diperlukan keterampilan dan kehati-hatian tinggi dalam mengikuti prosedur di laboratorium karena RNA lebih mudah terdegradasi, khususnya sampel hasil kultur jaringan tanaman. Salah satu parameter yang digunakan pada pengecekan kualitas RNA total adalah RIN (RNA Integrity Number). Penelitian bertujuan mendapatkan metode ekstraksi RNA pada daun, kalus dan embrio somatik kelapa sawit yang berkualitas baik dan memiliki konsentrasi tinggi untuk analisa transkriptomika.  Ekstraksi RNA dilakukan menggunakan metode kit Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) dan RibospinTM Plant (Geneall). Hasil menunjukkan bahwa RNA daun, kalus dan embrio somatik kelapa sawit telah berhasil diekstraksi dengan menggunakan kit RibospinTM (Geneall). RNA hasil ekstraksi tersebut dapat digunakan untuk sekuensing RNA dengan tujuan analisis transkriptomika, dilihat dari jumlah total RNA yang lebih dari 4 μg dan nilai RIN lebih dari 7. Kata Kunci: analisis RNA, embrio somatic, kalus, kelapa sawit, kualitas RNA 
A REVISED METHOD FOR SUCKER STERILIZATION TO SUPPORT THE IN VITRO PROPAGATION OF SAGO PALM (Metroxylon sagu Rottb.) Tajuddin, Teuku; ., Karyanti; Sukarnih, Tati; Haska, Nadirman
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.57 KB) | DOI: 10.29122/jbbi.v1i1.548

Abstract

Hutan sagu (Metroxylon sagu Rottb.) dapat ditemukan dalam area yang cukup luas di wilayah Maluku dan Papua. Besarnya keanekaragaman hayati dari pohon sagu dapat dilihat di areal ini. Pohon sagu tumbuh secara alami terutama di daerah dataran atau rawa dengan sumber yang air melimpah. Tanaman sagu dapat diperbanyak dengan metode generatif melalui biji, dan vegetatif melalui tunas anakan. Dalam rangka mendukung perbanyakan pohon induk yang unggul secara in vitro dalam skala besar, perbaikan metode sterilisasi tunas anakan mutlak diperlukan. Tunas anakan muda (15-20 cm) yang diperoleh dari Propinsi Papua digunakan sebagai eksplan. Tujuan percobaan sterilisasi ini dilakukan untuk mendukung perbanyakan pohon sagu secara in vitro. Pada percobaan ini antibiotik digunakan untuk membersihkan jaringan internal eksplan dari jamur dan bakteri. Hasil percobaan ini menunjukkan bahwa campuran alkohol dan antibiotik dapat menekan pertumbuhan kontaminan.Kata kunci: Antibiotik, kontaminan jamur dan bakteri, kultur in vitro, metode sterilisasi, sagu ABSTRACTNatural sago (Metroxylon sagu Rottb.) forest can be found in large area in Maluku and Papua regions. There are wide genetic diversities of sago palm found in these areas. This palm grows along riverbanks and in swampy areas which are not suitable for other crops. Sago palm is propagated generatively by seed and vegetatively by suckers. With the purpose of establishing the in vitro culture method for a large-scale of mass clonally propagation of superior genotypes of sago palm, generating sterilized explants are very important. Young suckers (15-20 cm) obtained from areas of Papua Province were used as explants. The sterilization experiments were carrying out to support the tissue culture of sago palm. Sterilization was conducted using antibiotics in order to get rid of fungi and bacteria from inner part of explants tissues. The results showed that from all sterilization methods tested, the best result was treatment using alcohol and antibiotic as disinfectant agents.Keywords: Antibiotics, fungi and bacteria contaminants, in vitro culture, sterilization method, sago palm
Appendix JBBI Vol 4, No 2, December 2017: Keyword Index and Author Index Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 2 (2017): December 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.005 KB) | DOI: 10.29122/jbbi.v4i2.2619

Abstract

EKSTRAK DAUN SEMBUNG (Blumea balsamifera) MEMPERBAIKI HISTOLOGI TESTIS TIKUS WISTAR YANG DIINDUKSI PAKAN TINGGI LEMAK Widhiantara, I Gede; Permatasari, Anak Agung Ayu Putri; Siswanto, Ferbian Milas; Dewi, Ni Putu Eny Sulistya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1063.993 KB) | DOI: 10.29122/jbbi.v5i2.2868

Abstract

Sembung (Blumea balsamifera) Leaf Extract Improves Testis Histology of High-Fat Diet-Induced RatsABSTRACTHigh fat and high cholesterol diet cause hyperlipidemia, leading to various health problems including reproductive health. The purpose of this study was to examine the effect of sembung (Blumea balsamifera) leaf extract on testicular histology profile of high-fat-diet-induced wistar. This research used 16 adult male rats (Rattus norvegicus), aged 3-4 month, weighing 150-200 g, and randomly divided into two groups. Eight rats were treated with distilled water and eight rats were treated with 2 mg/mL B. balsamifera extract. High-fat diet was a 30 days of porcine fat feed. The results showed that the diameter of seminiferous tubules, the number of spermatogenic cells of spermatogonium A, spermatocytes Pakiten and spermatid 16 were increased by giving sembung leaf extract for 50 days (p <0.05). These results suggested that the sembung leaf extract improves testis histology of high-fat diet-induced rats. Keywords: high-fat diet, rat, sembung leaf, seminiferous tubules, spermatogenic cellsABSTRAKMakanan tinggi lemak dan tinggi kolestrol menyebabkan hiperlipidemia yang menimbulkan masalah pada sistem reproduksi. Penelitian ini bertujuan untuk mengetahui manfaat pemberian ekstrak daun sembung (Blumea balsamifera) terhadap profil tubulus seminiferus dan sel-sel spermatogenik pada tikus wistar yang diinduksi pakan tinggi lemak. Sebanyak 16 ekor tikus wistar jantan dewasa (Rattus norvegicus) umur 3-4 bulan, berat 150-200 g, secara random dibagi dua kelompok, yaitu 8 ekor tikus kelompok kontrol (aquades steril) dan 8 ekor tikus kelompok perlakuan (ekstrak daun sembung dosis 2 mg/mL). Tikus diinduksi pakan tinggi lemak berupa lemak babi selama 30 hari. Pada prettest dilakukan pemeriksaan diameter tubulus seminiferus dan sel-sel spermatogenik. Hasil penelitian menunjukkan bahwa rata-rata diameter tubulus seminiferus tikus meningkat signifikan setelah pemberian ekstrak daun sembung. Peningkatan secara signifikan juga diikuti oleh spermatogonium A, spermatosit Pakiten dan spermatid 16 (p<0,05). Dapat disimpulkan bahwa ekstrak daun sembung memperbaiki histologi testis tikus wistar yang diinduksi pakan tinggi lemak.Kata Kunci: daun sembung, pakan tinggi lemak, sel spermatogenik, tikus, tubulus seminiferus

Page 22 of 55 | Total Record : 542

 20   21   22   23   24 

Filter by Year

2014 2023


Reset
Filter By Issues
All Issue Vol. 10 No. 1 (2023): Juni 2023 Vol. 9 No. 2 (2022): December 2022 Vol. 9 No. 1 (2022): June 2022 Vol. 8 No. 2 (2021): December 2021 Vol. 8 No. 1 (2021): June 2021 Vol. 7 No. 2 (2020): December 2020 Vol. 7 No. 1 (2020): June 2020 Vol. 6 No. 2 (2019): December 2019 Vol. 6 No. 1 (2019): June 2019 Vol 6, No 1 (2019): June 2019 Vol. 5 No. 2 (2018): December 2018 Vol 5, No 2 (2018): December 2018 Vol 5, No 1 (2018): June 2018 Vol. 5 No. 1 (2018): June 2018 Vol. 4 No. 2 (2017): December 2017 Vol 4, No 2 (2017): December 2017 Vol. 4 No. 1 (2017): June 2017 Vol 4, No 1 (2017): June 2017 Vol. 3 No. 1 (2016): June 20160 Vol 3, No 1 (2016): June 20160 Vol. 3 No. 2 (2016): December 2016 Vol 3, No 2 (2016): December 2016 Vol. 3 No. 1 (2016): June 2016 Vol 3, No 1 (2016): June 2016 Vol. 2 No. 2 (2015): December 20150 Vol 2, No 2 (2015): December 20150 Vol. 2 No. 1 (2015): June 20150 Vol 2, No 1 (2015): June 20150 Vol 2, No 2 (2015): December 2015 Vol. 2 No. 2 (2015): December 2015 Vol 2, No 1 (2015): June 2015 Vol. 2 No. 1 (2015): June 2015 Vol. 1 No. 1 (2014): December 20140 Vol 1, No 1 (2014): December 20140 Vol 1, No 1 (2014): December 2014 Vol. 1 No. 1 (2014): December 2014 More Issue