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BIOEDUSCIENCE
ISSN : 2614154X     EISSN : 26141558     DOI : -
BIOEDUSCIENCE is an open access journal that publishes research in the field of Biology and Biosain Education such as: Applied and implemented in education and learning, Botany, Zoology, Microbiology, Ecology, Biotechnology, Molecular Biology, Genetics, Bioinformatics, Cell and Developmental Biology, Biodiversity and Bioconservation. BIOEDUSCIENCE is published by Biology Education Study Program, Universitas Muhammadiyah Prof. Dr. Hamka, Indonesia regularly in June and December. ISSN : 2614-1558; P-ISSN : 2614-154X.
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Articles 11 Documents
Search results for , issue "Vol 9 No 3 (2025): BIOEDUSCIENCE" : 11 Documents clear
Early Detection of Pathogenic Fungi Curvularia sp. on Rice Seeds (Oryza sativa) Based on Modified Infrared Image Analysis Anggraini, Novita; Nirwanto, Herry; Mujoko, Tri
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/17568

Abstract

Background: Curvularia sp. is a seed-borne pathogenic fungus that can reduce rice plant productivity. At the same time, conventional seed health testing methods still rely on visual observation and require a relatively long incubation time. This study aims to evaluate the potential of infrared image analysis based on a modified imaging system for early detection of Curvularia sp. infection in rice seeds. Methods: Seed health testing was performed using the blotter test method. At the same time, image acquisition was performed with a digital microscope equipped with an infrared light source, and images were analyzed using pseudocoloring and RGB-based color segmentation. Results: The results showed differences in infrared signal intensity patterns in Curvularia sp.-inoculated seeds, which could be identified on the fourth day after inoculation, earlier than visual observation, which showed symptoms on the fifth day. Detection accuracy was calculated using a confusion matrix based on visual observation as the reference method, with a sample size of 50 seeds per observation day, yielding an average detection accuracy of 91% over seven days of observation. Conclusions: The modified infrared image analysis method has the potential to serve as an early detection method for Curvularia sp. infection in rice seeds, although its performance depends on the limitations of the imaging system and the validation method used.
The Effect of Insect Flour on The Growth Media of Beauveria bassiana in Controlling the Pupa Stage of Bactrocera carambolae Choerunnisa, Agustya Salma; Rahmadhini, Noni; Windriyanti, Wiwin
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/17713

Abstract

Background: One effective method for controlling Bactrocera carambolae is the use of Beauveria bassiana. However, a common challenge is the decline in the conidial quality and virulence due to the lack of chitin and protein sources. The insect Tenebrio mollitor and the pupal casings of Hermetia illucens are rich in chitin and have the potential to enhance the growth medium. Methods: The study used RAKF consisting of 3 factors and 3 replications. The first factor was the Type of Flour (T) consisting of T1: T. mollitor and T2: H. illucens. The second factor was Flour Concentration (K), which consisted of K1: 0.5% K2: 1% and K3: 1.5%. The third factor was Drying Temperature (S), of S1: 1000C S2: 1100C and S3: 1200C. Results: The results of the study show that the treatment caused symptoms of infection in the form of mycelium growth on the pupal cuticle and abnormalities in the emergence imago. The best combination is T2K2S2 (1% H. illucens flour at 1100C) which gives a mortality rate up to 76.7%, pupal duration of 9.3 days, and viability rate of 92.2%. Conclusions: The combination of H. illucens flour at a concentration of 1% and a drying temperature of 1100C is quite effective in increasing the ability of B. bassiana in controlling B. carambolae pupae in vitro.
In Vitro Antifungal Activity of Ethanolic Stem Extract of Tinospora crispa Against Candida albicans and Malassezia furfur Mumtaz, Syifa Afifah; Zulfa, Fajriati; Thadeus, Maria Selvester; Setyaningsih, Yuni
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/17984

Abstract

Background: Fungal infections such as candidiasis caused by Candida albicans and pityriasis versicolor caused by Malassezia furfur are highly prevalent in Indonesia. Conventional antifungal treatments often face challenges like resistance and adverse side effects. As an alternative, brotowali stem (Tinospora crispa) extract, rich in alkaloids, flavonoids, and tannins, shows promise as an antifungal agent. This research investigates the in vitro antifungal efficacy of ethanol extract from brotowali stem against C. albicans and M. furfur. Methods: A laboratory experimental design utilizing the well-diffusion method was applied, measuring inhibition zone diameters on Sabouraud Dextrose Agar after 2x24 hours at extract concentrations of 45%, 60%, 75%, and 90%. Results: The ethanol extract of brotowali stem exhibited statistically significant but relatively low antifungal activity based on inhibition zone diameters against C. albicans (0,002) and M. furfur (0,001), with higher concentrations showing greater antifungal. The largest inhibition zones measured 5.93 mm for C. albicans and 3.97 mm for M. furfur. Conclusions: The extract concentrations of 90% concentration exhibited moderate antifungal activity against C. albicans. For M. furfur, all concentrations demonstrated weak antifungal activity.
The Effect of Packaging and Encapsulation Temperature of Red Chili Seeds with Trichoderma sp. on Viability and Inhibition of Fusarium sp. Ratu, Dewanggie Sasmita; Nirwanto, Hery; Mujoko, Tri
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18020

Abstract

Background: Fusarium sp. is one of the main threats to chili cultivation, thereby reducing its economic value. Therefore, the use of biological microorganisms, such as Trichoderma sp., is an environmentally friendly alternative for controlling Fusarium sp. Application techniques for Trichoderma sp. biological agents include seed coating or encapsulation. However, ensuring the quality and effectiveness of encapsulated products during storage and distribution remains a concern. Thus, this study aims to examine the effects of packaging type and storage temperature on the viability and efficacy of Trichoderma sp. biological agents encapsulated in red chili seeds (Capsicum annuum L.) for inhibiting Fusarium sp. Methods: This study applied a completely randomized design (CRD) factorial with two factors, namely packaging material (aluminum foil and plastic) and storage temperature (5°C, 28°C, and 36°C), resulting in 7 treatments with three replications plus a control, resulting in a total of 21 experimental units. Results: Aluminum foil packaging stored at 28°C showed the highest viability of Trichoderma sp. and the most effective antagonistic activity against Fusarium sp. Conclusion: These results provide a sustainable, environmentally friendly solution for biological-agent-based seed storage.
Diversity of Fern Types (Pteridophyta) in The Grojogan Sewu, Parang Ijo, and Jumog Waterfall Tourist Areas of Karanganyar Regency Sholikah, Siti Amaliatus; Muzzazinah , Muzzazinah
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18421

Abstract

Background: The condition of the area in the three waterfall areas in Karanganyar Regency is very suitable for the growth of various organisms, both flora and fauna, especially ferns. These three areas have different environmental factors and regional topography, which allow various types of Pteridophyta to occur. This study aims to determine differences in fern diversity across three waterfall locations in Karanganyar Regency: Grojogan Sewu, Jumog, and Parang Ijo. Method: This research uses a purposive sampling method with plot and roaming techniques. Results: The research identified 35 terrestrial and epiphytic Pteridophyta, comprising 15 families and 6 orders: Polypodiales, Cyatheales, Gleicheniales, Lindseales, Salviniales, and Marattiales. The highest diversity index (H') and evenness (E) of ferns are found in the Parang Ijo Waterfall area, which are 2.14 and 0.71. The Parang Ijo Waterfall area has various environmental and topographic factors that support fern adaptation. The highest dominance index (C) is 0.51 in the Grojogan Sewu Waterfall area. Conclusion: The Grojogan Sewu Waterfall area has a more homogeneous and extensive habitat, thus supporting the dominant types of ferns to grow in large numbers.
Pengaruh Dosis Pupuk Silika terhadap Pertumbuhan dan Hasil Tiga Varietas Kacang Hijau (Vigna radiata) Nurwendah, Miftah Azzura; Koentjoro, Yonny; Suhardjono, Hadi
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18459

Abstract

Background: Mung beans (Vigna radiata) are one of the plants cultivated in Indonesia because they have many benefits, so cultivating green beans can be a profitable prospect. The use of superior varieties can increase better production results, in addition to the use of additional fertilizer besides basic fertilizer. Silica fertilizer is a fertilizer that has benefits for improving harvest quality, strengthening plant stems, etc. This study examined by 2 treatments: the dose of silica and 3 types of mung bean plant varieties, which aiming to identify the interaction and the right combination between the dose of silica fertilizer and superior varieties of mung beans to produce better production results. Method: This study was using a two-factorial completely randomized design. The first factor is silica dosage (Si0 : 0cc/l; Si1 : 1cc/l; Si2 : 2cc/l; and Si3 : 3cc/l) and the second factor is mung bean variety (V1 : Vima-1; V1 : Vima-2 ; and V1: Kutilang). Results: The results of the study revealed that Si2V2 treatment is the most effective to increase the production of green bean plants. Conclusion: The applications of silica fertilizer and superior varieties of mung beans had a significant impact on the observed parameters and the best results combination was revealed in the Si2V2 treatment (Silica 2cc and Vima-2).
The Effect of Problem Oriented Project Based Learning Model Assisted by Artificial Intelligence in Biology Learning on Science Literacy and Collaboration Skills of Grade XI MA Students Adawiyah, Rabiatul; Ibrohim, Ibrohim; Prabanintyas, Sitoresmi
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18707

Abstract

Background: Science literacy and collaboration skills are skills that students must be empowered with in the 21st century. Science literacy refers to understanding science issues and applying them in everyday life, while collaboration skills relate to the ability to work together to achieve goals or solve problems. Students must have these abilities so that empowering them can be done by applying innovative learning methods, strategies or models. POPBL is one of the models that can develop the skills needed by students. The effectiveness of the POPBL model is expected to increase with the integration of Aritifical Intelligence (AI). The role of AI as a tool to facilitate students in learning. This study aims to determine the effect of the AI-assisted POPBL model on science literacy and collaboration skills on circulatory system and respiratory system material. Methods: The research method used was quasi-experiment with nonrandomised control group pretest-posttest design. Involving three treatment classes namely POPBL class assisted by AI, POPBL, and regular class (lecture and assignment method). The instruments used were science literacy questions and collaboration skills questionnaire. Data analysis used ANCOVA test, before that the prerequisite test was carried out, namely homogeneity test and normality test. Results: The prerequisite tests conducted proved that the data were normally distributed and the data variance was homogeneous. The results of the ANCOVA data analysis value have a significance value of the learning model treatment which is p-value = 0.000. This shows that AI-assisted POPBL has an effect on science literacy and collaboration skills Conclusion: AI-assisted POPBL has an effect on science literacy and collaboration skills.
Production and Characterization of Nanoparticles from Citronella (Cymbopogon nardus) Extract Based on Chitosan Compounds Damayanti, Alfa Dirsista; Rahmadhini, Noni; Kusuma, Ramadhani Mahendra
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18769

Abstract

Background: The use of nanoparticles in Indonesia has been developing and is now being applied across sectors, including agriculture, health, and industry. Nanoparticles derived from Cymbopogon nardus can be used as nanopesticides because they contain several compounds that suppress pathogen growth and reduce pest populations. Chitosan is an additional compound used in the fabrication of these nanoparticles. Methods: This research used materials and tools, including a PSA (Particle Size Analyzer), a SEM (Scanning Electron Microscope), Cymbopogon nardus, and chitosan. The research stages included extraction of Cymbopogon nardus, nanoparticle fabrication, PSA analysis, zeta potential measurement, and SEM observation. Results: The PSA test revealed that the Cymbopogon nardus nanoparticles had an average size of 907.2 nm. The zeta potential of the nanoparticles was –40.62 mV, indicating good electrostatic stability. SEM images showed that the nanoparticles had an irregular shape. Conclusions: The Cymbopogon nardus nanoparticles produced in this study, as characterized by PSA, zeta potential, and SEM analyses, demonstrated the expected characteristics of nanoparticles.
Carbon Dynamics in Various Agroforestry Systems: A Study of Agrosilvopastural and Two Types of Agrosilvikultural Systems Wafiqah, Hidayatul; Widjajani, Bakti Wisnu; Wijayanti, Fitri
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/19941

Abstract

Background: Global climate change significantly affects carbon dynamics in perennial crop ecosystems, including agroforestry systems. This study aims to analyze carbon dynamics in three types of agroforestry systems: agrosilvopastoral, agrosilvicultural based on horticultural crops, agrosilvicultural based on woody plants, and to identify the diversity and types of vegetation. Methods: The study was conducted from January to March 2025 using field surveys and purposive sampling in Wonosalam, East Java, Indonesia. Laboratory analyses included physical properties (soil texture and bulk density), chemical properties. Results: The regression model showed R2 =0,419 Idicating that 41,9% of soil orgnaik carbon variation is explained by plant biomass carbon stock, highlighting its significant contribution to soil carbon accumulation. Conclusions: The agrosilvicultural system based on woody plants provides the highest contribution to carbon dynamics compared to other types of agroforestry, due to its vegetation composition dominated by woody and perennial plants. The even distribution of the Importance Value Index (IVI) reflects a stable and balanced ecosystem, as observed in plot 3, where excelsa coffee (Coffea excelsa) has an IVI of 37.78%, Durian (Durio zibethinus) has 35.56%, and banana (Musa spp) also has 37.78%. Keywords: Agroforestry; Biomass; Carbon Dynamics
Pengaruh Ekstrak Daun Kelor sebagai Priming Organik terhadap Perkecambahan Biji Kopi Arabika Sari, Fanny Septiani; Triani, Nova; Djarwatiningsih, Djarwatiningsih
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/20002

Abstract

Background: Hard Arabica coffee seeds cause slow germination, but this can be accelerated by increasing their viability through seed priming techniques. The objective of this study was to determine the effect of concentration and duration of immersion in an organic priming solution extracted from moringa leaves on germination results and to identify the appropriate concentration and duration of immersion. Methods: This study was conducted from October to December 2024 at the Greenhouse in Sidorejo Village, Krian District, Sidoarjo, and the Production Laboratory of the Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur. The study involved two treatment factors applied to Arabica coffee seeds: the concentration of the organic priming solution made from moringa leaf extract and the soaking duration. Results: The combination of treatment factors—concentration of moringa leaf extract solution and soaking duration—showed a significantly higher number of normal seedlings compared to abnormal seedlings, dead seeds, hard seeds, and fresh seeds. Conclusions: The optimal concentration of moringa leaf extract solution is 12%, and the optimal soaking duration is 38 hours

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