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Potensi Antibakteri Isolat Actinomycetes terhadap Aktivitas Proteolitik dan Amilolitik Escherichia Coli ATTC 25922 Bahar, Meiskha; Zulfa, Fajriati
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (546.699 KB) | DOI: 10.29238/teknolabjournal.v7i1.101

Abstract

Occurs E. coli resistance to class 3 cephalosporin class antibiotics and fluoroquinolone groups. The antibiotic resistance that occurs has narrowed the choice of therapy. This study aims to determine the effect of Actinomycetes isolates on proteolytic and amylolytic enzymes E. coli ATCC 25922. This research is experimental research, qualitative tests of protease and amylase enzymes from E. coli ATCC 25922 shown by clear zones around the growing colonies. The result of ANOVA One-Way test showed a significant difference in the width of clear zone, colony zone and PER and AER score with p-value < 0,05. This indicates that Actinomycetes isolates contain compounds that can act as inhibitors of protease and amylase enzymes from E.coli ATCC 25922. It is hoped that there will be research about the identification of Actinomycetes species isolates in Bogor Botanical Garden so that later can be cultivated and produced as an antibiotics alternative.
PKM PENINGKATAN PENGETAHUAN DAN KETRAMPILAN MEMBUAT MINUMAN JASULE (JAHE SUSU LEMON) PADA MASYARAKAT UNTUK MENINGKATKAN IMUNITAS DIMASA PANDEMI COVID-19 DI DESA PANGKALAN JATI CINERE DEPOK Susantiningsih, Tiwuk; Setyaningsih, Yuni; Irmarahayu, Agneta; Zulfa, Fajriati
Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Vol 5 No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jss.v5i2.234

Abstract

Dimasa pandemi Covid-19 ini, imunitas yang baik sangat diperlukan untuk menghindari tertularnya infeksi virus corona. Salah satu upaya mencegah infeksi virus corona adalah dengan meningkatkan status imunitas tubuh. Imunitas tubuh yang baik bisa didapatkan dari mengkonsumsi makanan sehat, istirahat yang cukup dan menghindari stress/tekanan batin. Satu kearifan lokal masyarakat di Indonesia dalam meningkatkan imunitas adalah kebiasaan mengkonsumsi minuman herbal yang dipercaya dapat meningkatkan imunitas tubuh karena terdapat beberapa kandungan yang sangat bermanfaat. Salah satu minuman herbal yang berasal dari bahan jahe dan lemon adalah Jasule yaitu minuman jahe susu dengan ditambahkan lemon. Dalam pelatihan ini peserta penyuluhan diberikan materi tentang pentingnya kandungan minuman herbal Jasule Jahe Susu Lemon. Pentingnya menanam jahe dan lemon sebagai salah satu tanaman obat di pekarangan. Serta diberikan pelatihan pembuatan minuman Jahe Susu Lemon sampai semua peserta benar benar bisa membuatnya sendiri. Kegiatan penyuluhan dan pelatihan pembuatan minuman herbal Jasule Jahe Susu Lemon untuk meningkatkan imunitas tubuh dimasa Pandemi Covid-19 dilaksanakan di Desa Pangkalan Jati Cinere Depok. Kegiatan ini dapat diterima dengan baik oleh peserta penyuluhan terlihat dengan antusiasme dan pertanyaan yang diajukan. Peserta mendapatkan tambahan pengetahuan dan ketrampilan dalam membuat minuman herbal Jasule Jahe Susu Lemon dapat dilihat dengan terdapatnya peningkatan nilai post test setelah dilakukan penyuluhan dan pelatihan pembuatan minuman herbal Jasule Jahe Susu Lemon untuk meningkatkan imunitas tubuh dimasa Pandemi Covid-19 di Desa Pangkalan Jati Cinere Depok sebesar 42,4% yaitu dari 47,9% menjadi 90,3%.
MANFAAT ASAM FOLAT UNTUK MENCEGAH OROFACIAL CLEFT PADA KOMUNITAS SATU SENYUM Fauziah, Cut; Thadeus, Maria Selvester; Bahar, Meiskha; Sukrisno, Adi; Zulfa, Fajriati
Jurnal Pengabdian Masyarakat AbdiMas Vol 8, No 03 (2022): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v8i03.5185

Abstract

Kasus bayi lahir dengan bibir sumbing masih banyak terjadi di Indonesia. Bibir sumbing merupakan kelainan berupa celah pada bibir atas. Celah ini bisa terjadi pada bagian langit-langit rongga mulut (cleft palate), bisa juga pada bagian bibir saja (cleft lip). Di beberapa kasus juga bisa terjadi pada kedua bagian. Namun pada umumnya, hampir separuh kasus bibir sumbing melibatkan celah pada bibir atas serta atap rongga mulut. Pemberian asam folat pada ibu hamil bertujuan untuk mencegah terjadinya kelainan kongenital yang terjadi selama masa perkembangan embryo , khususnya di trimester pertama. Kelainan kongenital yang selama ini berhubungan dengan kondisi folat ibu adalah Neural Tube Defect. Penelitian penelitian selanjutnya juga menyatakan bahwa ternyata asam folat juga berpengaruh terhadap kegagalan pembentukan jantung yang sempurna, kejadian celah bibir dan/atau langit. Metode penyuluhan dilakukan dengan metode ceramah secara daring via zoom, menggunakan slide power point tentang manfaat asam folat bagi ibu ibu anggota komunitas yang masih ingin memiliki anak dan tentang mempersiapkan kehamilan yang sehat dan menghadapi seribu hari pertama kehidupan. Keberhasilan dari kegiatan ini adalah terpenuhinya target dari jumlah peserta, serta ketercapaian tujuan dari kegiatan penyuluhan ini juga dapat dikatakan baik dari hasil pretest dan postest dan ketercapaian target materi  (100%).  Kesimpulan  dari  kegiatan  ini  adalah  terjadi peningkatan pengetahuan pada  ibu ibu anggota komunitas dapat  tentang manfaat asam folat untuk pencegahan orofacial cleft pada kehamilan berikutnya. Kata Kunci: Asam folat, orofacial cleft, komunitas
Nicotiana tabacum var. Virginia Bio Oil-based Pyrolysis Extraction Have Prominence Antimicrobial Potential Compared to Ethanol Heat Reflux Extraction (EHRE) Andri Pramesyanti Pramono; Basra Ahmad Amru; Halimah Anggi Rahmani; Sheila Azelya Fernanda; Yudhi Nugraha; Muhammad Yusuf Arya Ramadhan; Andre Fahriz Perdana Harahap; Ahmad Fauzantoro; Nasihin Saud Irsyad; Meiskha Bahar; Oktania Sandra Puspita; Fajriati Zulfa; Kori Yati; Mahdi Jufri; Misri Gozan
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.515-525

Abstract

Tobacco leaf contains antibacterial secondary metabolite compounds, such as phenol, alkaloids, and essential oils. This study compares the potential antibacterial effects of Indonesian tobacco leaf extracted using the heat reflux method (producing an extract) and pyrolysis method (providing a bio-oil). The tobacco leaf extract was challenged against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853. The bio-oil from the pyrolysis method showed inhibitory Kirby Bauer zones higher than those of the extract from heat reflux method, with the maximum results in the pyrolysis method indicating zones of 6.35 mm (S. aureus), 5.90 mm (E. faecalis), 3.97 mm (E. coli), and 5.025 mm (P. aeruginosa). Further study analyzed the effectiveness of the disc and well diffusion antibacterial test methods for measuring the antibacterial effect of bio-oils against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. The bio-oil used in the well diffusion test method showed the most significant antibacterial effectiveness. It showed the biggest inhibition zone, with a maximum of 11.65 mm and 8.90 mm for E. coli and P. aeruginosa. Our results showed Nicotiana tabacum var. Virginia Bio Oil from Ponorogo (Indonesia) is a strong potential antimicrobial, especially using well diffusion test.
Peningkatan Ketrampilan Membuat Minuman Jasule Untuk Imunitas Dimasa Pandemi Covid-19 Di Pangkalan Jati Cinere Depok Tiwuk Susantiningsih; Yuni Setyaningsih; Agneta Irmarahayu; Fajriati Zulfa
Jurnal Pemberdayaan Masyarakat Vol 6 No 1 (2021): Mei
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jpm.v6i1.5056

Abstract

During the Covid-19 pandemic, good immunity was needed to avoid the corona virus infection. One of the efforts to prevent corona virus infection was to increase the body's immune status. High body immunity can be obtained from eating healthy foods, getting adequate rest and avoiding stress / mental stress. One local wisdom of the people in Indonesia to increasing immunity level was the habit of consuming herbal drinks which were believed to increase immunity level because there were several very useful ingredients. One of the herbal drinks made from ginger and lemon was JASULE (Jahe Susu Lemon), which was a ginger milk drink with lemon added. In this training, the participants were given material about the importance of the herbal drink JASULE (Jahe Susu Lemon). The importance of growing ginger and lemon as a medicinal plant in the yard. Also given training in making JASULE drink until all participants can make it themselves. The training activities for making herbal drinks JASULE to increase body immunity level during the Covid-19 Pandemic were held in Pangkalan Jati Cinere, Depok. This activity was well received by all participants with enthusiasm and the questions asked. Participants gained additional knowledge and skills in making herbal drinks JASULE, which can be seen from the increasing in post test scores after counseling and training in making herbal drink JASULE to increasing body immunity level during the Covid-19 Pandemic in Pangkalan Jati Cinere, Depok was 42.4% ie from 47.9% to 90.3%.
Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study Muhammad Zaidan Tsani Ariandi; Meiskha Bahar; Hany Yusmaini; Fajriati Zulfa; Cut Fauziah; Andri Pramesyanti
Jurnal Biologi Tropis Vol. 21 No. 1 (2021): Januari - April
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i1.2466

Abstract

Actinomycetes are found in soils with loose, humus, dry characteristics and around plant roots. Actinomycetes produce secondary metabolite compounds as antibacterial. Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi are Gram negative bacteria that can cause infection in humans. This study aims to determine the ability of the Actinomycetes metabolite filtrate from Bogor Botanical Gardens as an antibacterial agent against the growth of E. coli, P.aeruginosa and S. typhi in vitro. This study used an experimental design with samples of Actinomycetes isolats originating from the Bogor Botanical Gardens soil using the disc diffusion method on Muller Hinton Agar (MHA) media by looking at the clear zone of bacterial growth around the disc paper. Of the three concentration groups, namely 50%, 60%, and 70%, the largest average inhibition zone is found at a concentration of 70% with the average for the three test bacteria E. coli, P.aeruginosa and S. typhi respectively: 4.23 mm; 3.0 mm and 8.43 mm. The results of the Kruskal - Wallis test with p value = 0.01 showed that there was an effect of the Actinomycetes metabolite filtrate on the growth of the three tested bacteria as antibacterials.  
Potensi Daya Hambat Filtrat Zat Metabolit Actinomycetes dari Kebun Raya Bogor terhadap Pertumbuhan Candida albicans dan Malassezia furfur Venita Octavia Tambunan; Meiskha Bahar; Andri Pramono; Cut Fauziah; Hany Yusmaini; Fajriati Zulfa
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 1 (2022): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i1.4792

Abstract

Candida albicans and Malassezia furfur are types of fungi that often cause infections in humans. Widespread fungal infections and lack of therapeutic options can develop fungal resistance and become serious problems in the future. Actinomycetes are Gram-positive bacteria that can produce secondary metabolites such as antifungal, antibacterial, and anticancer. The study aimed to determine the effectiveness of the metabolite filtrate Actinomycetes as an antifungal agent against the in vitro growth of  C.albicans and  M. furfur. This type of research is a laboratory experimental study with a post-test group design only using metabolite concentrations Actinomycetes of 25%, 50%, 75%, 100%, antifungal testing is done using the agar diffusion method on Sabouraud Dextrose Agar Media. Of the four concentrations, the average diameter of the clear zone for C.albicans  is  11.65mm; 12.48mm; 13.63mm and 13.80mm and for M.furfur is  6,025mm; 8,05mm; 9,1mm  and  9,9mm. There was a significant difference between each treatment group with a p-value of < 0.05. The compound Metabolite filtrate  Actinomycetes derived from  Actinomycetes can potentially be antifungal with one of its mechanisms binding to ergosterol in the cell wall that will interfere with the integrity of the fungal cell wall.
MCKENZIE EXERCISE DAN WILLIAM’S FLEXION EXERCISE EFEKTIF MENURUNKAN INTENSITAS LOW BACK PAIN Muhammad Afrian Wiji Pratama; Nurfitri Bustamam; Fajriati Zulfa
Jurnal Kesehatan Kusuma Husada Vol. 12 No. 1, Januari 2021
Publisher : Universitas Kusuma Husada Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.608 KB) | DOI: 10.34035/jk.v12i1.547

Abstract

Low Back pain (LBP) merupakan sindroma klinis yang ditandai dengan nyeri pada regio punggung bawah dengan atau tanpa disertai nyeri tungkai. Pembatik berisiko mengalami LBP. McKenzie Exercise dan William’s Flexion Exercise merupakan back exercise yang mudah dilakukan. Penelitian ini bertujuan membandingkan efektivitas McKenzie Exercise dan William‘s Flexion Exercise untuk menurunkan intensitas LBP pada pembatik. Penelitian menggunakan two-group pretest-posttest design. Sebanyak 20 orang pembatik di Kampung Batik Giriloyo dipilih dengan teknik purposive sampling. Subjek penelitian dibagi menjadi dua, yaitu kelompok yang melakukan McKenzie Exercise dan kelompok yang melakukan William’s Flexion Exercise. Kedua kelompok melakukan latihan tersebut dengan frekuensi tiga kali seminggu selama dua minggu. Sebelum dan sesudah latihan, intensitas LBP diukur menggunakan Numeric Rating Scale. Uji Wilcoxon digunakan untuk mengetahui apakah terdapat perbedaan intensitas LBP sebelum dan sesudah latihan. Uji Mann-Whitney digunakan untuk mengetahui apakah terdapat perbedaan efektivitas McKenzie Exercise dan William’s Flexion Exercise dalam menurunkan intensitas LBP. Analisis data menunjukkan intensitas LBP pada kedua kelompok sebelum melakukan back exercise adalah 5 (4-6). Pada kelompok yang melakukan McKenzie Exercise maupun kelompok yang melakukan William’s Flexion Exercise didapatkan penurunan intensitas LBP menjadi 2 (1-3). Terdapat perbedaan intensitas LBP sebelum dan sesudah latihan pada kelompok McKenzie Exercise (p = 0,004) dan pada kelompok William Flexion Exercise (p = 0,004). Tidak ada perbedaan penurunan intensitas LBP antara kelompok McKenzie Exercise dan kelompok William’s Flexion Exercise (p = 0,328). Low Back Pain (LBP) is a clinical syndrome that consists of pain at the lower back region with or without limb pain. Batik workers were at risk of LBP. McKenzie Exercise and William’s Flexion Exercise are an easy back exercise. This study was aimed to compare the effectiveness of McKenzie Exercise and William’s Flexion Exercise for reducing LBP among batik workers. This study used a two-group pretest-posttest design. A total of 20 batik workers in Kampung Batik Giriloyo were selected by purposive sampling technique. The research subjects were divided into two, namely the group doing the McKenzie Exercise and the group doing the William’s Flexion Exercise. Both groups did the exercise three times a week for two weeks. Before and after the exercise, the intensity of LBP was measured using the Numeric Rating Scale. The Wilcoxon test was used to determine whether there were differences in LBP intensity before and after exercise. The Mann-Whitney test was used to determine whether there was a difference in the effectiveness of the McKenzie Exercise and William's Flexion Exercise in reducing LBP intensity. Data analysis showed LBP intensity in both groups before doing back exercise was 5 (4-6). In the group that did the McKenzie Exercise and the group that did the William’s Flexion Exercise, the LBP intensity decreased to 2 (1-3). There were differences in LBP intensity before and after exercise in the McKenzie Exercise group (p = 0.004) and the William’s Flexion Exercise group (p = 0.004). There was no difference in the decrease in LBP intensity between the McKenzie Exercise group and the William’s Flexion Exercise group (p = 0.328).
PELATIHAN PENGENALAN TANDA DINI DEMAM BERDARAH DENGUE PADA MASYARAKAT PESISIR DESA PABEAN UDIK INDRAMAYU Erna Harfiani; Sri Wahyuningsih; Hany Yusmaini; Meiskha Bahar; Fajriati Zulfa; Taufiq Fredrik Pasiak
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 7, No 1 (2023): March
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v7i1.12158

Abstract

ABSTRAKKasus Demam Berdarah Dengue (DBD) di Desa Pabean Udik Kabupaten Indramayu masih tinggi. Penyakit Demam Berdarah Dengue (DBD) merupakan penyakit menular yang masih banyak menimbulkan kesakitan dan kematian. Sampai saat ini belum ada pengobatan penyebab, hanya pengobatan simptomatis dan suportif. Tujuan kegiatan adalah untuk meningkatkan tingkat pengetahuan tentang DBD dan tanaman obat pada masyarakat pesisir desa Pabean Udik Indramayu sebagai upaya pengendalian dan pencegahan DBD. Metode kegiatan berupa penyuluhan tentang DBD dan tanaman TOGA, pelatihan pemantauan jentik (JUMANTIK), dan pemberian beberapa jenis TOGA untuk ditanam di lingkungan rumah. Sebelum kegiatan dilakukan pretes dan setelah penyuluhan dilakukan posttest untuk mengetahui keberhasilan kegiatan. Lokasi penelitian di Desa Pabean Udik Kabupaten Indramayu pada bulan Oktober 2022 dengan peserta berjumlah 30 orang dan menggunakan media penyuluhan PowerPoint. Hasil pengabdian didapatkan bahwa karakteristik peserta sebagian besar responden berusia 36-45 tahun (33,3%), berpendidikan SD (40,7%). Terdapat peningkatan tingkat pengetahuan 10,19 (16.79 %), dimana nilai rata-rata setelah promosi kesehatan 70,89 sedangkan nilai rata-rata sebelum kesehatan promosi 60,70. Pelatihan ini akan dapat membantu tercapainya lingkungan yang sehat sehingga nyamuk tidak berkembang biak dan kejadian demam berdarah akan dapat berkurang. Kata kunci: DBD; pengetahuan; promosi kesehatan ABSTRACTDengue Hemorrhagic Fever (DHF) is an infectious disease that still causes a lot of morbidity and mortality. Until now, there has been no causative treatment, only symptomatic and supportive treatment. According to a report from the Indramayu District Health Office, DHF cases in Pabean Udik Village are still high. The purpose of this study was to determine the level of knowledge before and after health promotion. This study used an observational analytic design with a cross-sectional approach. The research location was in Pabean Udik Village, Indramayu Regency, in October 2022. Respondents were residents of Pabean Udik Village, Indramayu Regency, totaling 30 people. The dependent variable is the level of knowledge. The independent variable is health promotion using PowerPoint. Data collection using a questionnaire. Most of the respondents were aged 36-45 years (33.3%), had primary school education (40.7%), The average value after health promotion was (Mean = 70.89; SD = 1.805; p = 0.000) while the average value before health promotion (Mean = 60.70; SD = 1.708 ;  p = 0.000) answers to questions that increased sharply about first aid if were felt by DHF syndrome. Health promotion increases the level of knowledge of the residents of Pabean Udik Village, Indramayu Regency Keywords: DHF; knowledge; health promotion
Ekstrak Aloe vera dan Isolat Actinomycetes Sebagai Antimikroba untuk Bakteri Escherichia coli Monica, Astried; Bahar, Meiskha; Pramesyanti, Andri; Fauziah, Cut; Yusmaini, Hany; Zulfa, Fajriati
2-TRIK: TUNAS-TUNAS RISET KESEHATAN Vol 13, No 3 (2023): Agustus 2023
Publisher : FORUM ILMIAH KESEHATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/2trik13305

Abstract

Escherichia coli is one of the causes of diarrhea among other causes of diarrhea. Researchers are searching for various types of antibiotics by utilizing bioactive compounds from other living creatures, both plants and animals, and even bacteria such as Actinomycetes and Aloe vera isolates which have been proven to reduce diarrheal diseases, especially those caused by Escherichia coli. The aim of this review was to understand the antibacterial activity of Aloe vera leaf extract and Actinomycetes isolates against Escherichia coli microbes. This study was classified as a systematic review by conducting a literature search from various databases, namely PubMed, Ebsco and National Library E-Resources. The literature was limited to discussions of Escherichia coli, Aloe vera and Actinomycetes. Furthermore, there were 6 articles reporting that Aloe vera could inhibit Escherichia coli and there were also 6 articles reporting that Actinomycetes could inhibit Escherichia coli. Furthermore, it was concluded that Actinomycetes isolates and Aloe vera extracts could be used as alternative antibacterials in the treatment of diarrhea, especially those caused by Escherichia coli.Keywords: Actinomycetes; Aloe vera; antimicrobial; Escherichia coli ABSTRAK Escherichia coli merupakan salah satu suatu penyebab terjadinya diare di antara penyebab diare yang lain. Para peneliti melakukan pencarian berbagai jenis antibiotik dengan memanfaatkan senyawa bioaktif dari sesama makhluk hidup, baik dari tumbuhan maupun dari hewan, bahkan bakteri seperti isolat Actinomycetes dan Aloe vera yang telah terbukti dapat mengurangi penyakit diare, terutama yang disebabkan oleh Escherichia coli. Tujuan dari review ini adalah memahami tentang aktivitas antibakteri ekstrak daun Aloe vera dan isolat Actinomycetes untuk mikroba Escherichia coli. Studi ini tergolong sebagai systematic review dengan melakukan pencarian literatur dari berbagai database yaitu PubMed, Ebsco dan E-Resources Perpusnas. Literatur dibatasi pada pembahasan tentang Escherichia coli, Aloe vera dan Actinomycetes. Selanjutnya didapatkan 6 artikel yang melaporkan bahwa Aloe vera dapat menghambat Escherichia coli dan didapatkan pula 6 artikel yang melaporkan bahwa Actinomycetes dapat menghambat Escherichia coli. Selanjutnya disimpulkan bahwa isolat Actinomycetes dan ekstrak Aloe vera dapat digunakan sebagai anti bakteri alternatif dalam pengobatan diare, khususnya yang disebabkan oleh Escherichia coli.Kata kunci: Actinomycetes; Aloe vera; antimikroba; Escherichia coli