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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Published by Universitas Jenderal Soedirman
ISSN : 08531629     EISSN : 08531629     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
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Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries) Herdis Herdis; Maman Surachman; Muhammad Rizal; Arief Boediono; Yulnawati Yulnawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.142

Abstract

This study was aimed to examine the quality of chilled-cement of Garut ram which was diluted with Tris extender and various trehalose concentrations.  Cement was collected from three mature rams using an artificial vagina once a week.  After initial evaluation, the cement was divided into four parts and diluted with Tris extender only (control), Tris extender + 0.20% (TR0.2), Tris extender + 0.40% (TR0.4), and Tris extender + 0.60% (TR0.6) trehalosa respectively.  The extended-cement was stored at 5oC.  Parameters of chilled-cement quality examined were percentage of sperm motility, live sperm, and intact plasma membrane (IPM), which were evaluated every day for four days.  The  results showed that addition of trehalose did not significantly improved  the percentage of sperm motility, live sperm, and IPM (P>0.05) of Garut ram sperm stored in refrigerator at 5oC for 4 days.  In conclusion, the addition of trehalose does not enhance the quality of chilled-cement of Garut ram.
Karakter Antomi Daun Tumbuhan Mangrove Akibat Pencemaran di Hutan Mangrove Kabupaten Cilacap Siti Samiyarsih; Tata Brata Suparjana; Juwarno Juwarno
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 1 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.1.288

Abstract

This research was aimed to observe the anatomical character of mangrove plants leave and examine the differences from leaves anatomical character of mangroves in Donan river and Tritih forest in Cilacap regency as an self-adaptation indicator. The method which is used was survey method by collecting leaves sample of each mangrove plants. The sample was preparated using embedding method, and stained with safranin 1% disolved in alcohol 70%. The parameter of variable which is observed in leaves anatomical character are thickness of leaves,  leaves mesophyll thickness, cuticle thickness, stomata length size, and amount of stomata and trichome per leaves unit area. The data were analyzed with t-test for examining anatomical character difference. The results showed that four usual types of mangrove plants such as Achantus ilicifolius, Rhizospora mucronata, Aegiceros corniculatum, and Bruguiera gymnorrhiza have similar leaves anatomical structure. The anatomical character from polluted habitat were different from unpolluted habitat. This differences was showed in leaves mesophyll thickness, cuticle thickness, stomata length size, and amount of stomata and trichome per mm2 leaves unit area. 
Respon beberapa Varietas Padi terhadap Stress Garam Sugiyono Sugiyono; Siti Samiyarsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 2 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.2.93

Abstract

A study on the Responses of Several Rice Varieties toward Salt Stress has been carried out with a view to: 1) study the effect of salt stress on the physiological and anatomical responses of several rice varieties; 2) determine salt tolerant rice variety(ies). Research results showed that there were both physiological and anatomical changes on salt-stressed rice.  Those changes were determined by the level of salt concentration given.  The higher the concentration the more obvious the changes appeared.  There was a dramatic decrease in the total chlorophyll content of the salt-stressed leaf, coupled by a sharp decrease in the chlorophyll a/b ratio. Salt stress also resulted in the decrease in mesophyll thickness, and in turn leads to the decrease of leaf’s length and width.  In general, salt stress resulted in the decrease of plant growth, as indicated by the decrease of plant’s fresh and dry weights, eventual plant’s death was therefore imminent. The rice varieties used were sensitive to salt stress since an exposure of 50 mM NaCl has already resulted in the interference of rice growth.
Isolasi, Populasi, dan Karakterisasi Bakteri Rhizobium pada Daerah Perakaran dan Tanah dari Bengkulu, Sumatra Sri Purwaningsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.191

Abstract

A study was conducted in order to know the isolation, population and characterization of Rhizobium bacteria on the soil and rhizosphere plant from Bengkulu, Sumatra. The purpose of the study to know the population of Rhizobium bacteria from rhizosphere plant, and the get pure culture.           The sample was taken from 25 sample soil from rhizosphere of plant. Isolation was done in Rhizobium standart medium (YEMA) and the population was done with plate count methods. Incubation in room temperature of 27-280 C, after 7 days.            The results showed that the population of Rhizobium bacteria the range 18-87 X 105 CFU/g soil. The highest population from the rhizosphere of Zea mays plant. Seventy five gave of pure culture.  The growth characteristic the culture pure was observed by using YEMA medium mixed respectively with Brom Thymol Blue and Congo Red as indicators. Seventy five gave of culture pure, nine isolates can be grouped slow growing, while sixty six can be grouped as fast growing.
Eksplorasi Bakteri yang Berasosiasi dengan Karang Lunak sebagai Alternatif Sumber Senyawa Bioaktif: Uji Hayati Antibakteri Esti Harpeni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.283

Abstract

Soft coral has a significant function in the ecology of coral reef which produces secondary metabolites. One of very serious problems with coral reef ecosystem related to secondary metabolite improvement was coral supply because of need of many soft corals producing a little amount of secondary metabolites. The purposes of this research were to isolate and to select soft coral bacteria that have ability to produce antibacterial having been used to inhibit Escherichia coli and Staphylococcus aureus. The research was conducted from March 31th to November 2nd, 2005 by using description method. Sampling was carried out at Pulau Tegal Waters on two different coral genera, namely Briareum and Sinularia at three different sites. Laboratory studies were performed at Environment and Healthy Monitoring Fish Laboratory at Lampung Marine Agricultural. The results showed that five of 125 isolates obtained had potential of producing antibacterial that have been used to inhibit E.coli and S. aureus. The coral ability to inhibit E.coli and S. aureus was indicated by inhibition zone around soft coral bacteria colonies. Morphology and biochemistry characterization of potentially inhibiting isolates against E. coli and S. aureus resulted in strains Staphylococcus sp. (isolate code B1I10), Plesiomonas sp. (isolate code B1I11), Actinobacillus sp. (isolate code B2I6), Actinomyces sp. (isolate code B1II4), and    Aerococcus sp. (isolate code S1III2). 
Kondisi Hutan Mangrove di Kawasan Taman Wisata Alam Teluk Youtefa, Kota Jayapura Baigo Hamuna; Annisa Novita Sari; Ruth Megawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1028.529 KB) | DOI: 10.20884/1.mib.2018.35.2.611

Abstract

Ekosistem mangrove memiliki keanekaragaman hayati baik dari manfaat ekologi maupun sosial. Kondisi mangrove di kawasan Taman Wisata Alam Teluk Youtefa, baik secara kualitatif maupun kuantitatif terus menurun dari tahun ke tahun. Penurunan kualitas mangrove menjadi perhatian serius seiring dengan penyusutan luasnya. Perubahan kerapatan tajuk merupakan salah satu indikasi untuk memantau kualitasnya. Seiring dengan berkembangnya citra satelit seri Landsat, maka pada penelitian ini dilakukan pengolahan citra Landsat 8 akuisisi tanggal 21 Januari 2017 untuk menganalisis sebaran dan kerapatan mangrove menggunakan analisis indeks vegetasi di Taman Wisata Alam Teluk Youtefa, Jayapura. Tahapan identifikasi mangrove dilakukan dengan menggunakan komposit band RGB 564, kemudian dilakukan pemisahan obyek mangrove, non-mangrove dan badan air dengan menggunakan metode klasifikasi supervised. Tahap selanjutnya adalah melakukan analisis kerapatan mangrove dengan menggunakan formula Normalized Difference Vegetation Index (NDVI). Hasil penelitian menunjukkan bahwa luasan mangrove di Taman Wisata Alam Teluk Youtefa sebesar 233,12 Ha dengan tingkat akurasi sebesar 85,45%. Hasil analisis indeks vegetasi pada area mangrove menunjukkan bahwa kondisi kerapatan mangrove didominasi dengan kerapatan sedang dengan luas 152,73 Ha, sedangkan mangrove kerapatan padat 38,63 Ha dan kerapatan jarang 41,76 Ha
Perkembangbiakan Perkici Pelangi (Trichoglossus haematodus) di Laboratorium Penangkaran W. Widodo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.175

Abstract

The research was conducted to understand the breeding aspects of the Rainbow Lorikeet (Trichoglossus haematodus), which were caged in flocks and as a pair, in two aviaries of the Animal House Laboratory of Zoological Division, the Research Center of Biology, Indonesian Institute of Sciences (LIPI), Cibinong. From January 2002 until January 2005, 11 birds of the Rainbow Lorikeets were used in this research,. The birds were divided into two groups, the first group (9 individuals)  were caged in the first aviary  and the second one (2 individuals) were caged in the second aviary. The  size of each aviary room (LxWxH) was 3.32 m x 1.82 m x 2.85 m. The aviary rooms were equiped with the roosting, nest boxes, feeding and drinking water places. Foods and water were given in cafetaria everyday, starting from about 08.00 a.m. The foods consisted of bird food/”pur” (150 grams), white bread (15 grams), cooked quail eggs (60-70 grams), the lampung bananas (300 grams), slices of corn (100 grams), bean sprouts (50 grams), and palm sugar (50 grams). All of the igredients were mixed with 450-500 ml water and were then pulverized. The birds were also given some vegetables. The results were as follows: the Rainbow Lorikeets caged in flocks have produced two chicks and those in a pair only produced one chick. The clutchs consisted of  1-2 eggs. Eggs were white, smooth and their shape ovale. Average weight of the eggs was 7-8 grams. Weight of day old chicks was about 8 grams, those chicks were still blind and with out featheres (“altricial”). Incubation periods was 25-26 days, and incubated either by the female or the male. Fledging period was 71 days or more. The growing of the featheres and young birds of the Rainbow Lorikeets will be also discussed in this paper.
Hubungan Kekerabatan Fenetik Jamur Shiitake (Lentinula edodes (Berk.) Pegler) berdasarkan Karakter Morfologi Nuraeni Ekowati; Rina Sri Kasiamdari; Nursamsi Pusposendjojo; C. J. Soegihardjo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.2.267

Abstract

Phenetic relationship of shiitake mushroom (Lentinula edodes (Berk.) Pegler) cultivated in Indonesia especially in Java is still undisclosed. In order to increase adaptability and high production most of cultivators in Indonesia make inter-isolates breeding. Consequently  L. edodes isolates grown in Indonesia may have morphological and genetic diversity. Diversity among the isolates of L.edodes can be determined by morphological and molecular assessments. Isolates originating from different locations can show specific morphological characters, and requires more in-depth assessment of the macroscopic and microscopic morphological characters. Four  L.edodes isolates from Malang, Cianjur, Lembang and Yogyakarta were understudy to determine their morphological characters and phenetic relationship. Degree of similarity between the isolates was carried out through numerical taxonomy approach and the data obtained were analyzed using the Numerical taxonomy program and multivariate analysis system, version 2.1. The results showed that there was diversity of morphology of the four isolates and based on the results of data analysis obtained, the degree of similarity between the isolates ranged between 83-95%. Isolates of L. edodes from Malang to Cianjur showed the closest phenetic relationship (95%) and isolates from Malang to Yogyakarta showed the most distant phenetic relationship (83%). Based on the morphological characters obtained from this research revealed the diversity and phenetic relationship among the four isolates of L. edodes.
Upaya Pemanfaatan Rekaman Suara Burung dan Analisis Spektrogram untuk Menyusun Metode Klasifikasi Berdasarkan Suara (Sonotaksonomi) Marwan Irwandi; A. Hadi Mahmud; Abdulah Abdulah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.79

Abstract

This research explores possibility to classify bird species based on it sound.  Sound data (time-domain) plotting and its spectrum (frequency domain) are used to identify five kind of bird consist of Loriculus galgulus, Chloropsis sonnerati, Pycnonotus melanicterus, Lanius schach, and Sturnus contra.  There are indications that exist of equality sound parameter from the samples such as number of element, number of peak spectrum, dominant frequency and maximum frequency. This result open the possibility to develop method of bird species classification based on bird sound (sono-taxonomy).
Perancangan Primer Oligonukleotida untuk Polimerisasi in Vitro Gen Sukrosa Sintase Pieter Agustinus Riupassa
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.071 KB) | DOI: 10.20884/1.mib.2009.26.3.182

Abstract

The most important problems in using polymerase chain reaction (PCR) are the efficiency of energy, cost and time due to gene amplification. Oligonucleotide primer design of sucrose synthase gene was conducted as a model of preliminary experiment to amplify gene using PCR. In plant cells, this gene plays an important role in carbohydrate metabolism, a sucrose molecule break down into glucose. This design involved some computer software as bioinformatics tools. Five data sequences of legumes were downloaded from gene bank using accession number of AF030231, AJ311496, X92378, X69773, and D10266 belongs to soybean, pea, alnus bean, fava bean, and mung bean, respectively. After sequences alignment, some conservative regions were determined as the basis to construct forward and reverse primer candidates. Furthermore, the candidates were tested for compatibility. The results showed that the oligonucleotide primers can amplify sucrose synthase gene with ± 1462 bp fragment size using 5’-AACTTTgTgCTTgA-3’ and 5’-TCCTTTgACTCCTTC-3’ for forward and reverse primer, respectively. Even the PCR process weren’t applied, those primers might be universal primers to amplify sucrose synthase gene of legume plants.

Page 12 of 35 | Total Record : 342

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