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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Published by Universitas Jenderal Soedirman
ISSN : 08531629     EISSN : 08531629     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
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Pengaruh Kadar Lemak Berbeda Terhadap Produksi dan Kualitas Telur Induk Ikan Nilem (Osteochilus hasselti) Hidayat Djajasewaka; Reza Samsudin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 2 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.2.274

Abstract

The study was conducted on the effect of different levels of  lipid in diet production and egg quality of Osteochilus broodstock reared in fiberglass. Broaders with 95 gram for females, were fed diets containing different levels of lipid, 4%,  6%, 8% and 10%. The protein contain of the diet for 42 %. The density of 20 females  per fiberglass and daily fed ratio of 1.0 – 2.0% of body weight. The results  indicated that all diet gave weight gain, fed convertion ratio, egg production, GSI, gonada weight and the containts  protein and lipid of Osteochilus eggs were not significantly different (P>0.05). The eggs content completely amino, fatty and glutamic acids, histidine, proline, linoleic and linolenic acids, leads to good taste and delicious of eggs.
Deteksi Gen Litik BRLF1 Epstein-Barr Virus pada Penderita Karsinoma Nasofaring Tri Yulia Ningsih; Daniel Joko Wahyono; Nur Signa Aini Gumilas
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.1.517

Abstract

Rosenmuller fossa. Epithelial malignancy is often found in Chinese populations and Southeast Asia including Indonesia. Undifferentiated nasopharyngeal carcinoma (NPC WHO-3) type is 100% associated with Epstein-Barr virus (EBV) infection. Bam-HI R Leftward Reading Frame 1 (BRLF1) lytic gene has an important function as a transition mediator of latent phase to the lytic phase in EBV cycle. Detection of BRLF1 gene by PCR can be used for NPC diagnosis. The aim of this study is to identify BRLF1 lytic genes as molecular markers of Epstein-Barr virus in nasopharyngeal carcinoma patients with conventional PCR method and to determine the sensitivity of conventional PCR method to detect BRLF1 gene. The research design was cross sectional study. A total of 22 DNA samples were isolated from venous blood of NPC patients from RSUD Prof dr Margono Soekarjo, Purwokerto with informed consent. BRLF1 gene identification is done with conventional PCR technique. The results of this research showed that BRLF1 genes as molecular markers lytic cycle of Epstein-Barr virus in nasopharyngeal carcinoma patients can be identified conventional PCR technique that will produced DNA 157 bp. BRLF1 gene was detected in 16 samples (72.73%) of 22 samples of this study.
Variasi Genetik pada Marga Dicksonia Koleksi Kebun Raya Bali – Deteksi dengan PCR - SSCP Wenni S. Lestari; Bayu Adjie
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 3 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.3.166

Abstract

Dicksonia is one of tree ferns species that can be found in Indonesian highlands. Due to their wide distribution this species was suggested to have genetic variation among their population. Samples from Bali, West Java (Cibodas) and East Nusa Tenggara were compared using the PCR-SSCP methods to detect genetic variation of trnL-trnF intergenic spacer from the cpDNA plastid genome. Sample from Bali and West Java (Cibodas) did not morphologically and genetically variated, but they were variated to samples from East Nusa Tenggara.
Kualitas Perairan di Selat Makassar Ditinjau dari Aspek Bakteriologi Djoko Hadi Kunarso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 1 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.1.258

Abstract

Makassar strait is one of the important and strategic straits in Indonesia as it constitutes interislands and international sealanes. Its water condition is under the influence of both Kalimantan and Sulawesi islands, so that the dynamics in the region of marine and coastal environment becomes more complex. A study on water quality of bacteriological points of view in Makassar strait was conducted in October 2003. Seawater and sediment samples were collected by using the Research Vessel Baruna Jaya VIII at 16 stations. The aims of this study is to find out the water quality based on the contents of coliform bcteria and pathogenic bacteria Salmonella and Vibrio. The analysis of coliform bacteria was conducted by using membrane filter technique, while the pathogenic bacteria Salmonella and Vibrio were cultivated in the specific media Selenith broth and TCBS agar. The results showed that coliform bacteria at the surface layers depth varied from 80– 824 CFU/100mL with an average of 245 CFU/100mL, while those at the middle layer varied from 0–60 CFU/100mL with an average of 23 CFU/100mL. Seven pathogenic bacteria were isolated from seawater sample, i.e. Citrobacter spp., Edwardsiella spp., Proteus spp., Pseudomonas spp., Providencia spp., Shigella spp. and Vibrio spp, while eight pathogenic bacteria were obtained at sediment sample, i.e. Aeromonas spp., Citrobacter spp., Proteus spp., Pseudomonas spp., Providencia spp., Shigella spp., Yersenia spp. and Vibrio spp. These results indicated that coliform bacteria content in Makassar strait is under the threshold of Indonesian standard, i.e. Ministry Decree of Environment No: 51, 2004. Vibrio spp. was the pathogenic bacteria found both from seawater and sediment samples. In general, however, pathogenic bacteria was dominated by low pathogenic bacteria. Therefore, it can be concluded that from bacteriological point of view, water quality of Makassar strait is still relatively good.
Keanekaragaman Jenis Amfibi untuk Mendukung Kegiatan Ekowisata di Desa Braja Harjosari Kabupaten Lampung Timur Angga Arista; Gunardi Djoko Winarno; Rudi Hilmanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.458

Abstract

Amphibian life in nature is an interesting case which can be an ecotourism interest. The life cycle as well as the uniqe behaviours of the amphibi, is an interesting case to support the ecotourism activities. This research intends to determine the richness of amphibian minds, the diversity of amphibian kinds, and the amphibian distribution in order to support the ecotourism activities and to determine the society perception in utilizing the amphibi for the ecotourism activities. A combination of line transect method and Visual Encounter Survey method were used to know the species richness diversity and the distribution. One score one indicator quisioner was used to know the society perception against the amphibi to support ecotourism. The research was conducted in Braja Harjasari Village, East Lampung Regency during March to April 2017. Data analyzing was used a Shannon-wiener and Margalef index. The sosciety perception was analyzed descriptively and showed in a chart. The result showed that the amphibian richness was 0,6 which mean low, the diversity was 1,0 which meant medium, the shannon-wiener and margalef index was 0,9 which mean low community was stable and each kind of the amphibi was distributed equally. The society perception against the amphibi to support the ecotourism activities was mostly positive.
Isolasi dan Identifikasi Jamur Endofit Tanaman Manggis (Garcinia mangostana L.) Iva Akmalasari; Endang Sri Purwati; Ratna Stia Dewi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.2.131

Abstract

Mangosteen (Garcinia mangostana L.) is one of Indonesia's tropical horticultural commodities with high economic value. Mangosteen also has a lot of functions such as a producer of a wide variety of functional compounds such as anticancer, antiviral, antibacterial and antifungal that have some bioactive component i.e., micotoxin, enzyme, and antibiotics. The research was carried out to investigate the endophyte fungi in mangosteen plant tissue. Endophyte fungal isolation from mangosteen plant tissue resultsed 11 isolates which have identified Phoma sp., Acremonium sp., 2 isolates of Penicillium sp., Geotrichum sp., Pestalotiopsis sp., Botryosphaeria sp., Colletotrichum sp., Chrysosporium sp., Aspergillus sp. and Blastomyces sp., and 2 isolates have not been identified.
Sexing pada Ikan Brek Puntius orphoides (Valenciennes, 1863) Menggunakan Metode Truss Morfometrics Suhestri Suryaningsih; Mahmed Sagi; Kamiso H. N.; Suwarno Hadisusanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.1.219

Abstract

The red chick barb (Puntius orphoides) is one of fresh water ichtiofauna included in family Cyprinidae found a lot in River Klawing, the biggest and richest river in Purbalingga with 18 species of fish.  The aim of this study was to find out the differences between male and female red chick barb by truss morphometrics and to find out truss morphometrics distance.  The material used were sex mature fish from River Klawing.  The variables meassured included morphometry based on truss morphometrics point, number of male and female fish.  Truss distance was then compared to the total length so that the truss distance ratio was determined.  Next, a statistical test i.e. t test was performed between the male and female fish.  The results of this study showed that the male and female red chick barb can be distinguished by truss morphometrics method.  The distance ratio to be used as diagnostic character were 11 and 24 ration of truss morphometrics distance compared found in the head, body, and tail.
Struktur Komunitas Kepiting Intertidal pada Mangrove yang Terdegradasi di Segara Anakan Cilacap Marista Zalindri; Moh. Husein Sastranegara
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.338

Abstract

Makrobenthos intertidal crabs were found living in the sediment and its presence greatly affect the primary productivity in the mangrove ecosystem of Segara Anakan Cilacap. This mangrove area is the largest in the island of Java.  Human activities have caused a lot of pressure. The objective of this study is 1) to determine the structure of the different intertidal crab communities on degraded mangrove; 2) to know the different sediment texture on degraded mangrove in Segara Anakan Cilacap. This research was carried in two mangrove areas based on mangrove canopy cover percentage namely 80% and 30% canopy cover.  The research method for crab community structure was survey conducted by cluster sampling method from January-March 2013. The structure of the intertidal crab of different communities in normal mangrove areas and damaged mangroves. In the normal mangrove area, the number of species found in normal intertidal crab included 15 species consisting of 592 individual, while in the damaged mangrove area there were 14 species consisting of 845 individual. The normal mangrove area has clayey silt sediment texture, while the damaged mangrove area has the sand sediment texture of silt clay.
Keanekaragaman Genetik Rayap Tanah Genus Coptotermes (Isoptera: Rhinotermitidae) di Pulau Jawa Niken Subekti; Dodi Nandika; Dedy Duryadi Solihin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.156

Abstract

Subterranean termite Coptotermes spp. has been known as the most economically important structural pest in Indonesia. Due to morphological ambiguity, traditional identification of Coptotermes spp. has always been difficult and unreliable.  Through molecular diagnostic method, a study was conducted to determine genetic variation of Coptotermes spp. occurring in Java Island.  Termite specimens were collected from Banten 1, Banten 2, DKI 1, DKI 2, Jabar 1, Jabar 2, Jateng 1, Jateng 2, Yogya 1, Yogya 2, Jatim 1, and Jatim 2.  The method for identification was PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis using four restriction enzymes each of which was applied to CO II amplicon for all the Coptotermes spp. being analyzed.  The results showed the existence of two species of Coptotermes in Java which are different from both Coptotermes gestroi and Coptotermes formosanus.
Dekolorisasi Beberapa Macam Limbah Cair Batik menggunakan Limbah Baglog Pleurotus ostreatus dengan Waktu Inkubasi Berbeda R. Roro Theresia Sorta; Sri Lestari; Ratna Stia Dewi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 3 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.3.249

Abstract

Liquid waste of batik is dyes waste that will pollute the environment, lowers the quality of water and are toxic to living things. Spent mushroom of  Pleurotus ostreatus can be used as an alternative method decolorize of batik liquid waste. The research aimed to determine the spent mushroom of P. ostreatus ability as an absorbent of Zn and decolorize agent of batik waste, i.e: napthol, indigosol and mixed waste. This research used experimental methods in Completely Randomized Design (CRD) with 9 treatments and 3 replicates. The results showed that the spent mushroom P. ostreatus was able to decolorizing some liquid waste of batik. Optimum incubation time on the decolorization liquid waste of batik on incubation time of 72 hours.

Page 13 of 35 | Total Record : 342

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