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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Published by Universitas Jenderal Soedirman
ISSN : 08531629     EISSN : 08531629     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
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Zoea Syndrome (ZS) pada Larva Udang Vannamei (Litopenaeus vannamei) Rubiyanto Widodo Haliman
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.77

Abstract

White shrimp (Litopenaeus vannamei) culture in Indonesia has been well developed since 1999.  Among the problems occurred during the culture period in the hatchery, Zoea Syndrome (ZS) is the most serious.  An observation has been done to determine the prevalence and the clinical signs of this syndrome. Nauplii were collected from hatcheries in East Java (3 hatcheries) and Central Java (1 hatchery) and subject to PCR test in order to obtain TSV free nauplii.  The results showed that TSV free nauplii could also be infected by ZS, where the prevalence could reach 100%.  There was also a possibility that Vibrio sp. was involved in  ZS infection.
Reproductive Characteristics and Induced Breeding in Heterobranchus longifilis Jojo Subagja; Rudhy Gustiano
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.180

Abstract

Two experiments were conducted in the IRD-Gamet Laboratory, Montpellier, France. The first was to evaluate the recovery of Heterobranchus longifilis female gonad after induced ovulation, under tropical condition. Oocyte diameter was used as indicator of the recovery rate following by induced breeding, hatching rate and abnormality of larvae. The results showed that the recovery was about 28 to 35 days after ovulation. The second was carried out to improve the milt production by hormone treatments. Volume of intratesticular sperm and the number of spermatozoa were observed. The results indicated that the treatmens did not prove any significant difference.
Analisis Variasi Bentuk Karapaks Kuya Batok (Cuora amboinensis Daudin, 1802) dan Bajuku (Orlitia borneensis Gray, 1873) dengan Metode Elliptic Fourier Descriptors Tony Febri Qurniawan; Dina Rusiana; Abdul Rachman
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 3 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.3.272

Abstract

Turtles is easily identified because of its shield. The dorsal shield or commonly called carapace is one of characteristics that can be used for turtle’s identification. People and researchers usually can distinguish the turtles just by looking at the carapace valve, but not in carapace shape. Both of them sometimes have their own interpretation about it, because carapace shape has a variation. This research was aimed to obtain some information about differences of carapace shape variation between Coura amboinensis and Orlitia borneensis quantitatively and also to investigate about characteristic of variation on carapace shape of a species. The specimens were taken from Pusat Penyelamatan Satwa Jogja. The picture of carapace shape of the turtles was changed into bitmap then processed used SHAPE ver 1.3. The variation of carapace contour were extracted by image processing, described and analyzed by Elliptic Fourier Descriptors. Mathematically independent shape characteristics were then indentified by Principal Component Analysis of The Elliptic Fourier Descriptors. Principal components represented the aspect ratio of the broad size, length size, and curve or roundness margin of the carapace. Based on result showed that intraspecies variation shape of carapace on C. amboinensis and on O. borneensis can be analysis into 3 principal component. The principal component 1st (PC1), 2nd (PC2) and 3rd (PC3) components accounted for 82.77%, 10.3% and 4.73%  of total shape variation on C. amboinensis. The 1st, 2nd and 3rd components accounted for 88.1%, 7.02% and 3.71% of total shape variation on O. borneensis. The contribution ratio of PC1 was over 80% on both of species, showing that most of the variation on carapace shape could be explained by the aspect ratio. The PC2 and PC3 were less than 20%, indicated that there is small asymmetric variation degree of roundness on carapace.
Ekspresi mRNA BRLF1 Virus Epstein-Barr dari Biopsi Jaringan Tumor Formalin-Fixed Paraffin Embedeed sebagai Petanda Biologi Molekul Diagnosis Karsinoma Nasofaring Arin Herkilini; Pancrasia Maria Hendrati; Nur Signa Aini Gumilas; Hidayat Sulistyo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.508

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor that grows in the nasopharynx with a predilection in the fossa Rosenmuller. Epithelial malignancies are often found in populations of China and Southeast Asia, including Indonesia. The NPC incidence in year 2008 as many as 84,400 cases and 51,600 of these cases resulted in death. A total of 120 new cases per year NPC found in hospitals Prof. dr. Margono Soekarjo (RSMS), Purwokerto. The NPC is difficult to be diagnose caused its primary tumor lies closed to the skull base as well as various structures of vital organs. Therefore, methods that can detect early NPC required for inspection.The etiology of NPC is multifactorial consisting of genetic factors, factors of infection Epstein-Barr Virus (EBV) and environmental factors.EBV has two phases in the cycle of infection that is the phase of lytic and latent phase.  BRLF1 has an important function as  mediator transition from latent e NPC. The research aimed  to analysis mRNA BRLF1 expression as a biomarker of  NPC diagnosis by RT-PCR and to determine the positivity of RT-PCR method to detect the expression of mRNA BRLF1. The research design was cross sectional study. Samples were FFPE tumor biopsy of NPC WHO III  and the total samples were 22 individu from Department of Pathology Anatomy,  Prof. Dr. Margono SoekarjoHospital, Purwokerto with informed consent. The positivity of mRNA BRLF1 from FFPE tumor biopsy of NPC WHO III  was in 63.6%indicating a high expression.
Komunitas Biota Hewan Bentik Pada Danau Paparan Banjir Di Kalimantan Timur Lukman Lukman; T Chrismadha; Muhammad Fakhrudin; J Sudarso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.192 KB) | DOI: 10.20884/1.mib.2009.26.3.164

Abstract

Pemacuan Pembungaan Tanaman Lengkeng (Euphoria longana Lam.) untuk Produksi Buah di Luar Musim Theresia Prawitasari; Aris Munandar; Mursal Mursal
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 2 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.2.256

Abstract

The objectives of this research were to analyze the effect of paclobutrazol and ethepon in substituting climate factors effect in flowering and fruiting processes of longan, to determine the application way and dozes of paclobutrazol, as well as the timing of ethepon application after paclobutrazol to stimulate ‘off-session’ longan fruiting. Three combined factors treatment namely: the way of paclobutrazol application (soil drench and foliar spray); dozes of paclobutrazol (control, 1 g, and 2 g paclobutrazol per tree); and the timing of ethepon application after paclobutrazol, were designed in randomized block design and applied at 20-30 years old longan trees, which were grouped into 3 group according to the amount of flushing twigs among all of the twigs in the whole canopy.     Result of the research showed that specific climate conditions (2-3 months dry which was followed by temperature decrease until 15-22oC) as pre condition for the happening of natural flowering on longan might be substituted by paclobutrazol and ethepon. The application of 1 g paclobutrazol in foliar spray method, which was followed by application of 400 mg/l ethepon 1 month after paclobutrazol, might stimulated 20-30 years old longan trees, flowering 37.5 day after paclobutrazol application, while control trees did not flower until 120 days observation.The trees got foliar spray application way of paclobutrazol was induced after 29.8 DAPA, while the trees under soil drench were induced after 42.0 DAPA. Dozes 1 and 2 g paclobutrazol induced flowering after 15.9 and 16.9 DAPA, while control trees were induced after 74.8 DAPA. The fastest achieving time of the induction stage (17.7 DAPA) were shown in the trees under foliar spray application way of 1 g paclobutrazol which was followed by the application of 400 mg/l ethepon 1 month after paclobutrazol application (C2D1W1). Furthermore, the plants under the same treatment combination were differentiated faster (37.5 DAPA). The fastest induction and differentiation stage achieving time were strongly related to the highly of the leaf chlorophyll content (1.33 mg/l) as well as C/N ratio (9.67) which were the highest value in both of the variables. The average of the blossom achieving time was 52.3 DAPA. The differentiation and the blossom stage were shown only in the plants were in flushing condition, with 25-75% of flushing twigs. The decrease of twigs long addition parallel to the paclobutrazol dozes and vice versa to the ethepon application time. The faster the ethepon application time, the higher the twigs long addition.               Finally, the result of the research showed that if the humidity of flowering and fruiting-plants canopy, as the result of paclobutrazol and ethepon application during the differentiation processes until ‘fruit set’ was keep protected, the retardant application in February (the middle of raining session in the research area) might supply longan fruits in June (the middle of dry session, when the longan fruits supply were very limited).
Intensitas dan Variasi Morfometrik Trichodina sp. pada Benih Ikan Gurami (Osphronemus gouramy Lacepede) Pendederan I yang Dijual di Pasar Ikan Purwonegoro Kabupaten Banjarnegara Nurul Anisah; Rokhmani Rokhmani; Edy Riwidiharso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.349

Abstract

Purwonegoro Fish Market, Banjarnegara district is the largest fish market in Central Java southern and western parts. This fish market serves as a center for buying and selling fish and fish seed traffic in a particular place to the location of the fish farmers. It will possibly spread a particular type of disease from one area to another. Trichodina sp. is found in the seed of gouramy fish hatchery center area with the pathogenicity level reached 80%. Trichodina sp. has a big role in the cultivation of gouramy production by reducing the physical condition of the fish so dangerous and cause death of gouramy in the seed phase. The results showed that as many as 150 gouramy fish fry were examined, 56 gouramy fish fry were infected Trichodina sp. All of Trichodina sp. are found as many as 573 individual from the gouramy seeds infected. Total intensity of Trichodina sp. from gouramy seeds amounted to 10.97 individual. The measurement results morphometric characters Trichodina sp. show the variation of morphometric with body diameter from 47.5 to 112.5 μm; denticle ring diameter from 22.5 to 47.5 μm; denticle diameter of 12.5 to 42.5 μm, adhesive disc diameter 37.5 to 65 μm; membrane width 2.5-7.5 μm and the number of denticle 13-28. Trichodina sp. allegedly found there are three types of T. heterodentata, T. nigra, and T. acuta.
Keanekaragaman Kultivar Salak Pondoh di Banjarnegara Annisaurrohmah Annisaurrohmah; Wiwik Herawati; Pudji Widodo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.2.216

Abstract

Snake fruit (Salacca zalacca (Gaertner) Voss) is originated from Indonesia. The aim of this study was to find out the cultivar diversity of salak pondoh in Banjarnegara, and to determine the similarity relatiinshipa between cultivars pondoh in Banjarnegara. The method used was exploratory survey with purposive random sampling. Morphological characteristics observed included 50 characters in the form of stems, leaves, flowers, fruits, seeds, and spines. Samples were taken at 5 districts namely Madukara, Banjarmangu, Sigaluh, Pagentan and Banjarnegara. The result of this research showed that there were six cultivars of Salacca zalacca namely cultivars ‘Pondoh Nglumut’, ‘Linting’, ‘Ketek’, ‘Manggala’, ‘Madu’, and ‘Super’. Whereas in S. zalacca 'Pondoh Ketek' has various number of seeds in the fruit. The most closely related cultivars were ‘Pondoh Nglumut’ and ‘Super’. The most distinct cultivars were S. zalacca ‘Manggala’ and ‘Ketek’.
Distribusi Syzygium cumini (L) Skeels di Aceh Besar Afridah Afridah Rosannah; Nursahara Pasaribu; Saleha Hannum
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.336

Abstract

The jamblang distribution has been studied in the southern part of  Aceh Besara, which is in the South of Jhanto district, Northern Krueng Raya, Southern Masjid Raya and Ujung Pancu district. The aims ofthis study was to determine the distribution area of jamblang based on rainfall, soil type, and lands cover . The data were collectedby observation. Distribution data are presented in the  form of map using ArcView 3.3 Software. The results of the analysis showed that the common jamblang was distributedin the rainfall region of 1500-2500 mm/year in dryland farming and the chromic luvisols, humic acricols, dystric fluvisols, dan rendzinas.
Genetic Variability in the Indonesian Giant Clam (Tridacna crocea and Tridacna maxima) Populations: Implication for Mariculture and Restocking Program Agus Nuryanto; Dedy Duryadi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 2 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.2.154

Abstract

Tridacna crocea and T. maxima are relatively abundant in the Indonesian coral reef. These two species are, however, under high presure due to exploitation for food, industry, and aquarium trade.  It is, therefore, necessary to understand their biology, such as genetic variability within and between populations, before utilizing them for strain improvement and restocking, prior to the extinction of the populations of T. crocea and T. maxima.  Here we amplified a length of 456 bp of the mitochondrial DNA cytochrome c oxidase I gene from Tridacna crocea and of 484 bp from T. maxima to asses the genetic variability within and between populations of both species.  The results showed that both species have high genetic diversity and polymorphism within each local population. This provides a sufficient basis for selection of improved strain of T. crocea and T. maxima for mariculture. However, if the genetic variation led to genetic differentiation among populations due to the result of evolutionary adaptation, mixing genetically different populations may result in the break up of co-adaptation gene complexes.  This might result in the loss of the physiological capacities of the parental populations.

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