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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Published by Universitas Jenderal Soedirman
ISSN : 08531629     EISSN : 08531629     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
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Induksi Pematangan Gonad dengan Ovaprim-C: untuk Penyediaan Benih Belut Sawah (Monopterus albus) Berkelanjutan Priyo Susatyo; Sugiharto Sugiharto; Elly Tuti Winarni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 3 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.3.247

Abstract

Rice eel (Monopterus albus Zuiew), have economical value is high in Banyumas, national to international markets, but rarely is grown intensively, the seed is still dependent on the catch. Reproduction of swamp eel unlike other fish species in the presence of phase teleostei  intersex / hermafroditus and in nature only spawn once a year. This study will seek to accelerate the scale seed production with natural canvas with several techniques triggers lust and sex organ maturation eels based preparedness aspects of reproductive anatomy, hormones estradiol and testosterone profiles and the intrinsic need for hormonal regulation and the mechanism for the reproductive cycle of the eel rice. In the first year aims to determine (1) the profile of estradiol and testosterone hormonal parent during the natural reproductive cycle, (2) histologic gonadogenesis (development of the testes and ovaries) eel rice during the natural reproductive cycle was evaluated by measuring fecundity. Results: In the control group, body weight gradually decreased during the study. Unlike the control group, the treatment groups were observed every two weeks showed an increase in body weight; Results of the study, in the control group (A0), the value of female eels GSI average of the first two weeks until the fourth two weeks is 0.67 % and 1.78%. While the treatment group A1 was 1.42% - 4.28% (DM-1 s / d DM-4), for the treatment A2 GSI average value is 2.52% - 7.05%; fecundity eggs increased during induction period; calibration titer two types of hormones in the first two weeks to the fourth two weeks showed improvement when compared with the control group; histological profile of rice eel gonad after induction of ovaprim-C show that up to the fourth two weeks, gonad has reached late-stage yolk globule (advanced primary oocyte), even the most mature oocyte has reached the stage: in male eels up to the fourth two weeks has reached the stage of spermatozoa
Serapan Fosfor dan Pertumbuhan Kedelai(Glycine max) pada Tanah Ultisol dengan Pemberian Asam Humat Wahyuningsih Wahyuningsih; Elly Proklamasiningsih; Murni Dwiati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.911 KB) | DOI: 10.20884/1.mib.2016.33.2.345

Abstract

Ultisol merupakan salah satu jenis tanah asam yang memiliki kelarutan Al, Fe, dan Mn tinggi, serta kandungan P dan Moyang rendah. KelarutanAl dan Fe yangtinggiakan menjerap fosfat, sehingga ketersediaan dan serapan P bagi tanaman menjadi rendah. Salah satu upaya untuk mengatasi ketidaktersediaan P pada tanah Ultisol yaitu dengan penambahan asam humat. Asam humat merupakan makromolekul polielektrolit yang memiliki gugus fungsional seperti -COOH, -OH fenolat, maupun -OH alkoholat,sehingga asam humat memiliki kemampuan untuk membentuk kompleks dengan ion logam. Penelitian ini dilakukan dengan tujuan untuk: (1) mengetahui pengaruh asam humat terhadap serapan P dan pertumbuhan tanaman kedelai (2) menentukan konsentrasi asam humat yang paling baikdalam meningkatkan serapan P dan pertumbuhan tanaman kedelai. Metode penelitian yang digunakan adalah eksperimental dengan Rancangan Acak Kelompok (RAK). Pengelompokan didasarkan atas perbedaan intensitas cahaya yang ada di rumah kaca. Perlakuan berupa konsentrasi asam humat, dengan 5 konsentrasi yang berbeda yaitu 0 ppm, 400 ppm, 800 ppm, 1200 ppm dan 1600 ppm. Parameter yang diukur meliputi tinggi tanaman, luas daun, bobot basah, dan bobot kering, P tersedia serta P tanaman. Data yang diperoleh dianalisis menggunakananalisis ragam pada tingkat kepercayaan 95% dan 99%. Apabila hasil perlakuan berpengaruh nyata, maka dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa asam humat berpengaruh terhadap serapan P dan pertumbuhan kedelai pada tanah Ultisol. Asam humat 1200 ppm merupakan perlakuan paling baik dalam meningkatkan ketersediaan P, sehingga mampu meningkatkanpertumbuhan kedelai pada tanah Ultisol.
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhyncos) Setelah Penyimpanan dalam Medium Berbeda Dikombinasi Krioprotektan Kuning Telur Berbagai Konsentrasi Yusni Atifah; Dadang Mulyadi Saleh; Hendro Pramono; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.118

Abstract

The purpose of this study is to asses motility and viability of domestic duck (Anas platyrhyncos) spermatozoa after 6 day storing in preservation medium of different egg yolk cryoprotectant concentration in three different medium Tris, PBS or Ringer lactate. In this experiment, we applied 3 combination of factors as treatments : extender (Tris, PBS or Ringer lactate), egg yolk (EY) concentration (0, 5, 10, 15 or 25%) and storage time (0,1,2,3,4,5,6 days). Spermatozoa was obtained by massage technique, ejaculate then were swup up in the extender, the swup-up sperm then were incubated in preservation medium as each treatments subjected to motility and viability assesment before and after storage at refrigerator temperature (50C). The results showed that sperm motility and viability were highly significantly different (P<0.01) among 107 treatments. Extender factor as well as storage duration time were highly significantly different (P<0.01) affected the motility and viability of the treated sperm. EY concentration was significantly (P<0.05) affected viability, however there were no factor (P>0.05) affected motility of the sperm. The best treatment resulted in promising quality having the highest motility of 46,67 ± 32,15 5 motile was from the treatment of 10% EY in PBS stored for 4 days. The best viability result was from the tretament of 25% EY in PBS after 4 days storage resulted in 50,00 ± 36,05 % of viabie sperm. In conclusion this protocol could be applied for artificial insemination trial.
Pemetaan Distribusi dan Densitas Monyet Hitam Sulawesi (Macaca Nigra) di Sulawesi Utara Saroyo Saroyo; Roni Koneri
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 3 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.3.203

Abstract

A study on mapping of distribution and population of Sulawesi crested black macaques (Macaca nigra) has been carried out to evaluate the current condition of distribution and density. This research was conducted from January to November 2009 at 24 locations in North Sulawesi Province. Determination of  M. nigra distribution was based on the information provided by people surrounding the sites and field observation. Line transect was used to evaluate the density of the monkey with the length based on the habitat condition and the width was 100 m. The results showed that monkeys were not found at all of the locations and based on categories, high density of monkey was only found at Tangkoko-Batuangus Nature Reserve  Two serious problems responsible to monkey population decreasing were hunting for consumption and habitat destruction. It can be concluded that distribution of M. nigra in North Sulawesi is meta-population and the current status of the species as critically endangered is accepted; the conservation of this species depends on the active management and terminating the factors of population decreasing.
Keragaman Plankton dan Kondisi Perairan Tambak Intensif dan Tradisional di Probolinggo Jawa Timur Utojo Utojo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 2 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.2.299

Abstract

This study aims to estimate the wealth and stability of traditional and intensive brackishwater pond waters through biological index calculation (diversity, uniformity and dominance of plankton) in traditional and intensive brackishwater pond waters of Probolinggo Regency, East Java Province. Sampling plankton and water from the site, considered to represent the diversity of plankton and water stability of traditional and intensive brackishwater pond. Plankton was collected using a plankton net no. 25, then preserved using 1% lugol solution. Measurement of water quality variables include temperature, salinity, dissolved oxygen and pH, whereas the laboratory analyzes that Total Organic Matter (TOM), NO2, NO3, NH3, PO4, Total Suspended Solids (TSS), and Fe. Plankton identification using microscope and calculation with cell counting method. The results of analysis of plankton in intensive brackishwater pond waters are obtained as much as 23 genera consisting of 16 genera of phytoplankton were included into three classes, namely Bacillariophyceae as much as 5 genera, Cyanophyceae 3 genera, Dinophyceae and Chlorophyceae, each of the four genera, whereas zooplankton consisting of 7 genera are included into two classes, namely Crustaceae 6 genera and Rotatoria 1 genus. In traditional brackishwater pond earned as much as 13 genera consisting of 8 genera of phytoplankton were included into Bacillariophyceae 5 genera, Cyanophyceae 2 genera, and Dinophyceae 3 genera, whereas zooplankton consisting of three genera are included into class Crustaceae 2 genera and Rotatoria 1 genus. Plankton abundance in intensive brackishwater pond ranges 702 - 4269 ind./L, diversity index of 0.26 -2.38, uniformity index of 0.10 - 1.5, and dominance index of 0.02 - 0.68. In traditional brackishwater pond have an abundance of plankton range 134 - 776 ind./L, diversity index of 0.10 - 1.97, uniformity index of 0.08 - 1.0, and dominance index of 0.01 - 0.75. Traditional and intensive brackishwater pond waters condition still within the limits that can be tolerated by the culture organism. Based on the index value of biological, diversity of genus in the intensive brackishwater pond is relatively higher than traditional brackishwater pond and the community stability moderate, whereas traditional brackishwater pond unstable. On average, the uniformity of the genus in both  brackishwater ponds are relatively evenly and genus of plankton to dominate the others genus.
Aktivitas Harian Monyet Hitam Sulawesi (Macaca nigra) di Cagar Alam Tangkoko-Batuangus, Sulawesi Utara Saroyo Saroyo; Sri Supraptini Mansjoer; Rudy C Tarumingkeng; Dedy Duryadi Solihin; Kunio Watanabe
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.145

Abstract

This study was conducted on Rambo II Group at Tangkoko-Batuangus Natural Preservation, North Sulawesi, from January to February 2004. Data were collected using scan sampling with two minute interval. The results showed that the daily activities of the monkey group consisted of feeding and foraging (34.55%), moving (18.70%), resting (28.65%), and social behaviour (18.10%). It could be concluded that there was no difference between the daily activities of Rambo II Group and those of Rambo Group, the original group before being fragmented into Rambo I and Rambo II Groups.
Biologi Ikan Uceng (Nemachilus fasciatus C.V.) di Sungai Banjaran Kabupaten Banyumas Slamet Risyanto; Erwin Riyanto Ardli; Isdy Sulistiyo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 1 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.1.235

Abstract

A research on fish biology Nemachilus fasciatus CV was conducted in River Banjaran Banyumas. The purpose of this study was to assess the biological aspects, including the nature of growth, Gonado somatic index, fecundity, sex ratio and natural food habits. The method used is survey, the technique used was stratified random sampling.  Banjaran river was divided into nine stations: three stations on the upstream side of the river, three stations in the middle of the river and three stations on the river downstream. The results showed the data aspects of the biology of N. fasciatus in rivers Banjaran was allometrik growth, the gonado somatic index ranged from 5.40 to 17.46%. The highest fecundity of 7,567 items found in fish N. fasciatus which has a total length of 74 mm and 3.25 g body weight. Fecundity was as low as 1,665 points with fish body length of 58 mm and 2.02 g body weight.  The sex ratio is 1:1,25.  The natural food with the highest frequency of occurrence was on Pediastrum and low frequency of occurrence is in Cosmarium and Bronchionus.
Analisis Fenetik Kultivar Krisan (Chrysanthemum morifolium Ramat.) Indah Anugrahsari; Sukarsa Sukarsa; Siti Samiyarsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.1.325

Abstract

Chrysanth (Chrysanthemum morifolium Ramat.) atau dikenal sebagai Seruni merupakan tanaman herba hias. Chrysanth merupakan salah satu komoditas tanaman hias utama yang memiliki keragaman kultivar, seperti dapat dilihat dari bentuk dan variasi warna bunganya. Penelitian ini bertujuan untuk menyelidiki keragaman dan hubungan kesamaan kultivar chrysanth. Metode penelitian yang digunakan purposive sampling. Data dari chrysanth kultivar morfologi dan anatomi daun karakter dianalisis berdasarkan hubungan kemiripan dengan UPGMA (Unweighted Pasangan Metode Group dengan Arithmetic mean) metode menggunakan 5.05 software MEGA. Berdasarkan penelitian, delapan kultivar chrysanth diperoleh, yaitu C. morifolium 'Cut Nyak Dien', C. morifolium 'Dewi Ratih', C. morifolium 'Dwina Kencana', C. morifolium 'Dwina Pelangi', C. morifolium 'Elora ', C. morifolium' Pasopati ', C. morifolium' Puspita Nusantara ', dan C. morifolium' Swarna Kencana '. Kesamaan terdekat adalah antara C. morifolium 'Dwina Kencana' dan C. morifolium 'Dwina Pelangi' kultivar, dengan indeks ketidaksamaan terkecil 0,152, sedangkan kesamaan paling jauh adalah antara C. morifolium 'Elora' dan C. morifolium 'Swarna Kencana 'kultivar, dengan indeks ketidaksamaan terbesar 2,256.
Identifikasi Senyawa Alkaloid dari Akar Piper sarmentosum Roxb. Ex Hunter dan Uji Aktivitasnya terhadap Jamur Candida albicans Hartiwi Diastuti; Sadiah Achmad; Enny Ratnaningsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 2 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.2.96

Abstract

Piper sarmentosum Roxb. Ex Hunter or “Sirih duduk” has long been used for traditional medicine to cure various diseases, such as fungus infections. The investigation of the bioactive compounds of P. sarmentosum roots has not been carried out. This research was aimed to isolate the bioactive compounds from P. sarmentosum roots. The results showed that methanol extracts of P. sarmentosum roots have an activity on Candida albicans. The separation a bioactive compounds from methanol extracts of P. sarmentosum roots was performed by column chromatography, thin layer chromatography and recrystalizations. The identifications of the bioactive compounds were carried out using ultra violet spectrometry, infrared spectrometry, gas chromatography-mass spectrometry and nuclear magnetic resonance spectrometry. The results indicated that from methanol extracts, an alkaloid compound of piperoylpyrol derivative was 5-hydroxy-5- (3,4-methylenedioxyphenyl)-2-pentenoyl pyrol, could be purely isolated. Examination of bioactivity at concentration 0.10-2.50 mg/ml showed that this compound had an activity on C. albicans.
Pola Dominansi Capit pada Uca spp. (Dekapoda:Ocypodidae) Dewi Citra Murniati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 2 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.2.194

Abstract

Uca spp. are unique crab species marked with asymmetry cheliped. Major and minor cheliped has different function. The size of Major cheliped are several times of the minor ones (cheliped dominance pattern). Each species has different pattern of cheliped dominance. This study was to know the pattern of cheliped dominance on three Uca species. Major and minor cheliped length from twenty male individuals of U. dussumieri, U. vocans and U. perplexa were examined. The pattern of Cheliped dominance was obtained by comparing the major cheliped length to minor cheliped length. The two-tailed single sample t test showed similar cheliped dominance pattern within Uca species (P>0.001). While Kruskal-Wallis test showed different cheliped dominance pattern between Uca species (P>0.001). The cheliped dominance pattern of U. dussumieri, U. vocans and U. perplexa are 5.09, 4.24 and 4.75.

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