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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 342 Documents
Molecular Analysis of Synedrela Nodiflora (L.) Gaertn. Resistance Against Fomesafen using RAPD Markers Murni Dwiati; Agus Hery Susanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.290

Abstract

Both morphological and protein studies reveal that differences are observed between resistant Synedrella nodiflora against fomesafen and its susceptible wildtype. These are, however, more or less influenced by environmental factors, so that molecular analysis employing DNA markers is necessarily required. The methods involved total genomic DNA extraction using modified CTAB protocol following Doyle & Doyle (1990), RAPD marker amplification and visualization of RAPD markers prior to data analysis. Ten RAPD markers were used, but only seven of them showed polymorphism.  Calculation of genetic distance and variation was carried out employing PopGen software.Based on the RAPD markers used in this study, it can be concluded that genetic distance between susceptible and resistant S. nodiflora is higher than that within susceptible samples supporting our previous morphological and protein data, although genetic variation among susceptible individuals seems to be significantly high.
Distribusi dan Jenis Sampah Laut serta Hubungannya terhadap Ekosistem Terumbu Karang Pulau Pramuka, Panggang, Air, dan Kotok Besar di Kepulauan Seribu Jakarta Yayan Mardiansyah Assuyuti; Reza Bayu Zikrillah; Muhammad Arif Tanzil; Azkiya Banata; Pangestuti Utami
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (984.15 KB) | DOI: 10.20884/1.mib.2018.35.2.707

Abstract

Ekosistem terumbu karang di kepulauan Seribu mengalami dampak dari kegiatan manusia seperti sampah laut. Penelitian ini dilakukan di pulau Pramuka, Panggang dan Air pada bulan Maret 2015 dan Kotok Besar pada bulan Juli 2016, kepulauan Seribu, Jakarta. Pengambilan data penutupan terumbu karang dan jumlah sampah dilakukan di dua kedalaman yaitu 3-5 m dan 10-13 m dengan 4 stasiun, kecuali Kotok Besar dengan 2 stasiun dan 1 kedalaman. Tujuan dari penelitian ini adalah mengetahui distribusi dan jenis sampah laut, persentasi penutupan substrat terumbu karang, faktor kimia fisik perairan dan hubungan sampah terhadap penutupan substrat terumbu karang. Hasil penelitian menunjukkan jenis sampah laut yang paling banyak ditemukan adalah plastik di kedalaman 3m yang berada di pulau Pramuka dan Panggang. Regresi linear jumlah sampah dengan terumbu karang terdapat di kedalaman 3m di tiga pulau dan 10m di satu pulau. Persentasi penutupan substrat terumbu karang di dua kedalaman dan masing-masing pulau tidak berbeda nyata. Hal ini karena faktor kimia fisik perairan mendukung terhadap pertumbuhan terumbu karang. 
Kondisi Terumbu Karang di Pulau Sembilan Kabupaten Sinjai Sulawesi Selatan Suharyanto Suharyanto; Utojo Utojo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.178

Abstract

Sembilan Island waters is one of the catch fishery production centers in Bone Bay, South Sulawesi. It was informed that the capture has decreased since 1999, which might be caused by  coral reef damage although the damage precentage was still unknown. An observation on coral reef condition was conducted in the waters of Sembilan Island, South Sulawesi, on June and November 2000 using scuba diving set and under water writing tools. Monitoring of coral reef condition was conducted in 7 m depth up to 100 m length of shore line in seven stations employing a line intercept transect method. The data of coral reef were analysed by using dBase III and lifeform programs. The results showed that living coral around station I, III, and IV were generaly in good condition except in the stations V, VI and VII, which were in middle condition and in station II, which was in bad condition. The lifeform of coral reef in Sembilan Island were predominated by Acropora Branching (ACB), Nonacropora Branching (CB), Coreal Foliose (CF), and Coral Massive (CM) meanwhile other benthic fauna were predominated by Soft Coral (SF) and Sponge (SP).
Pengaruh Pemupukan terhadap Pertumbuhan Bibit Garut (Maranta arundinacea L.) di Bawah Naungan 50% Ninik Setyowati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 3 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.3.270

Abstract

A study on the effect of fertilizer on the growth of arrowroot (Maranta arundinacea L.) seedling under 50% shading was conducted in the Experimental Garden of Research Center for Biology-LIPI, Cibinong. The research was conducted with Factorial patterns in Randomized Completely Block Design with 2 factors and 5 replications.  The first treatment was fertilizing with 2 levels of factors i.e. W1 = 1x fertilizing in the early of planting and W2 = 2x fertilizer (the early and 5 month age of seedling). The second treatment was the kinds of fertilizer with 5 levels of factors i.e. P1 = Control (without fertilizer), P2 = manure of goat dunk, P3 = manure of chicken dung, P4 = manure of cow dung, P5 = compost fertilizer.  The result showed that two time application of manure of goat dung (P2) is the best for growing the arrowroot seedling (98.6 cm of plant height, 100 sheet of leaf number, and 8 clumps).  The lowest data was seen at control with 1 time fertilizing i.e. 39.6 cm of plant height, 28 sheet of leaf number, but the clump is 3.2 more than the compost fertilizer (2.8 of clumps). This treatment was also seen to increase the plant height adding of arrowroot seedling during observation.  The frequency of fertilizer was significantly effect the growth of vegetative parts of plant, i.e. 2x fertilizer better than 1x fertilizer.
Stok Rumput Laut Alami di Perairan Teluk Prigi Trenggalek Jawa-Timur Achmad Kadi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.341

Abstract

Wild seaweed is natural source in term of seed supply on aquaculture activity. In recent years, seaweed is the important aquaculture comodity from Indonesian provinces.  Observation on wild seaweed in Prigi Bay, conducted in may 2011. The aim of the observation was to find out the condition and to measure the density of seaweed in Prigi Bay-Trenggalek East Java waters. Observation was carried out through survey method. Five observation stations were made the distance within the station was 1000 m parallel  with the beach line, then divided in 100 m to become 10 applying points on each stations. Quadrat transect in located perpendicular from the beach line to slope area, 1x1m² frame placed on each 10 meter by Saito and Watanabe. Species biodiversity, density and dominancy were measure from raw data obtained and using the computing formula according Michael, Kusmana, Soerianegara and Indrawan. Three species were found: Chlorophytaceae and Phaeophytaceae. The total of wild seaweeds in density of observation site was 239,53 g/m² and Padina australis has the highest density among other ie 82,01 g/m² and dominant value of the species was 30,23 g/m².  Seaweed which have economic value in observation site was Caulerpa, Halimeda, Padina and Sargassum.
Isolasi dan Karakterisasi Mikrob Pendegradasi Senyawa Nitril Ahmad Thontowi; Bambang Sunarko
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.87

Abstract

Biotransformation of nitrile compound using microbe represents the best way in eliminating  toxicity. Besides, microbes and enzymes of nitriles degradation can be used in synthesis of various chemical compounds with a high economic value. The purpose of this research is to obtain microbial potential for nitriles degradation, to obtain information of physiological of chosen microbe and the characteristic of its enzyme. We have conducted are isolation and selection of microbial nitriles degradation, perception of growth pattern, determination of influence various and concentration of nitriles on microbial growth, determination of enzymes specificity and inductivity, and determination of optimum pH and temperature. The result of microbial isolation from various sources of waste obtained by 12 isolates at acetonitrile, 18 isolates at benzonitrile, 8 isolates at adiponitrile, 1 isolate at lactonitrile, and 1 isolate at ß- aminopropionitrile. GLB5 isolate was selected from 46 isolates, because this isolate can grow at some tested nitriles. The best growth of GLB5 isolate in acetonitrile 360 mM, benzonitrile 30 mM, 3-sianopiridin 15 mM, and ß- aminopropionitrile 140 mM. The growth of GLB5 isolate in acetonitrile 360 mM were experienced a lag phase during 8 hours, and an exponential phase during 40 hours. Time of cell doubling during 7 hours and 42 minutes, and specific growth rate (µ) equal to 0,09 h-1. The character of nitriles degradation enzymes from GLB5 whole cell was inductive, with optimum activity at pH and temperature equal to 7.2 and 40oC.
Hubungan Genetika Pria Masyarakat Sembiran dan Tri Wangsa di Bali I Ketut Junitha; Bambang Suryobroto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.185

Abstract

Balinese community has evolved since the prehistoric era. The present community is structured into Hinduism caste (Brahmana, Kesatria, Wesia and Sudra), but remnants of their ancient communities, collectively called Bali Aga, have not undergone this structure. The first three castes of modern Balinese that comprises Tri Wangsa gentry were derived from JavanesekingdomofMajapahit(1343 AD). One of Bali Aga communities is Sembiran of Northern Bali. Using chromosomal microsatellite DNA, allelic variability of Sembiran and Tri Wangsa communities was reported. Both of them had the same high frequency of alleles which suggested genetic intermixing or sharing of common ancestor. However, a few alleles were unique for each community. This uniqueness was related to patrilineal system of Balinese community and to high mutation rate of Y chromosomal microsatellite DNA.
Budidaya Ikan Kerapu (Serranidae) pada Keramba Jaring Apung (KJA) Langkosono Langkosono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 2 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.2.277

Abstract

Groupers are commercial fish belonging to Serranidae family.  Some species such as barramundi cod (Cromileptes altivelis) and flowery cod (Epinephelus fuscoguttatus) have been cultured in floating net cages. This study was carried out to determine the growth rate in terms of length and weight of both species as well as water quality of coastal Kombal Bay, West Pemenang Village, West Lombok. The results showed that the growth rate of both species was approximately the same as each other. Nevertheless, the weight increase of flowery cod seemed to be slightly faster than that of barramundi cod. Water quality in the location of the fish culture supported sufficiently  the growth of both grouper species.
Aktivitas Antioksidan Ekstrak Fenol Daun Gayam (Inocarpus fagiferus Fosb) Dwi Marga Lestari; Nurul Mahmudati; Sukarsono Sukarsono; Nurwidodo Nurwidodo; Husamah Husamah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.1.596

Abstract

This study aims to determine the total phenolic content and antioxidant activity in gayam leaf extract (Incarpus fagiferus Fobs). The research method used is a quasi-experiment that aims to predict the situation to be achieved through actual experiments but no treatment. The sample used is old gayam leaves, with the characteristic of dark green leaf and rough leaf surface. The process of preparing simplicia, ie preparing fresh gayam leaves, dried in an oven temperature 45-50oC, and then dried to produce gayam leaf powder. Samples were extracted with methanol solvent and ethanol for 5 days. The total phenol assay method uses Folin-Ciocalteau method and antioxidant activity test using DPPH free radical retardation method (1,1-diphenyl-2-picrylhydrazyl). The results showed that the total phenolic content of gayam leaf extract with ethanol and methanol solvent was 313,704 GAE (Gallic Acid Equivalent) and 273,913 GAE, respectively. Antioxidant activity as a free antidote to free radical DPPH is known to be valued with IC50 (inhibitory concentration).
Beberapa Faktor yang Berhubungan dengan Kontaminasi Mikroorganisme pada Jamu Gendong Di Kota Semarang Sulistiyani Sulistiyani; Siti Thomas Zulaikhah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 3 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.3.169

Abstract

The objective of this study was to know some factors related to microbial contamination in herbal medicine products at Semarang City. This study was conducted by descriptive method employing cross sectional design in which samples were taken by purposive random sampling. The results showed that Escherichia coli, Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa were identified. The number of fungi exceeded the standard indicating that 62.5% of the herbal medicines surveyed did not meet the required standard of Kepmenkes no. 661/Menkes/SK/VII/1994. The relationship between the quality of herbal row material, processing, and serving to the microbial contamination in herbal medicine products was observed.

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