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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 342 Documents
Biodiversitas Belalang (Acrididae : Ordo Orthoptera) pada Agroekosistem (Zea mays L.) dan Ekosistem Hutan Tanaman Bagas Prakoso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.490

Abstract

Penelitian ini bertujuan untuk mengetahui keanekaragaman belalang (Acrididae: Ordo Orthoptera) pada agroekosistem (Zea mays L.) dan ekosistem hutan tanaman serta menentukan peran belalang pada kedua ekosistem. Penelitian ini dilakukan dengan metode survai lapangan. Parameter yang diamati pada setiap lokasi meliputi keanekaragaman vegetasi tumbuhan, pengumpulan belalang Acrididae ordo Orthoptera dan pengamatan langsung terhadap belalang Acrididae ordo Orthoptera. Keanekaragaman belalang yang ditemukan pada ekosistem dianalisis dengan indeks keanekaragaman meliputi: indeks keanekaragaman (H’), kemerataan (E) dan indeks kesamaan sorensen (C) serta analisis korelasi dan regresi. Sampel diambil dari agroekosistem (Zea mays L.) dan ekosistem hutan tanaman yang selanjutnya diulang sebanyak empat kali. Penelitian dilakukan pada bulan April s/d Juli 2014. Hasil penelitian ini ditemukan sebanyak 3.097 individu Orthoptera yang termasuk dalam Famili Tetrigidae, Acrididae dan Pyrgomorphidae yang terdiri dari 7 genus yaitu Atractomorpha, Criotettix, Gesunola, Hesperotettix, Miramella, Oxya, dan Valanga dengan 7 spesies. Pada agroekosistem ditemukan 3 spesies dengan 1.030 individu sedangkan pada hutan tanaman ditemukan 5 spesies dengan  2.067 individu. Hasil nilai indeks keanekaragaman Shannon-Weinner pada ekosistem hutan tanaman nilai keanekaragamannya lebih tinggi (0,6307) jika dibandingkan dengan agroekosistem (0,5325). Berdasarkan hal tersebut maka ekosistem hutan tanaman memiliki biodiversitas belalang yang lebih tinggi daripada agroekosistem (Zea mays L.).
Isolasi dan Identifikasi Kapang Saprofitik pada Sampel Tanah di Sekitar Kawasan Gunung Gamalama, Ternate Muhammad Ilyas
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 3 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.3.204

Abstract

Microorganisms, such as mould and bacteria, have important roles in organic matter decomposition; and this process is fundamental to maintain soil structure and fertility. Moulds have hypha with filament structures that can penetrate to the substrates. Their enzymatic capability is higher than that of bacteria, especially in decomposing organic compounds like lignin and cellulose. The objective of the study was to isolate and identify saprophytic mould inhabiting soil of Mount Gamalama, Ternate, North Moluccas. The mould isolation was based on sample dillution method with Rose Bengal Cloramphenicol Agar isolation media. The abundance of saprophytic mould counted by measuring the average Colony Forming Unit (CFU)/ml of all mould colonies which grown on isolated media by Total Plate Count (TPC) method. The diversity of isolated mould was identified based on phenotypic characters by observing both macroscopic and microscopic morphology. The result showed that the average of mould colonies were between 13,30 x104 and 78,15 x104 CFU/ml. Identification based on morphology from selected mould isolates showed that 25 fungal taxa and two groups of unidentified fungal isolates were identified.
Beberapa Aspek Pertumbuhan Lamun Enhalus acoroides (Linn. F) Royle di Pulau Barrang Lompo Makassar Supriadi Supriadi; Dedi Soedharma; Richardus F Kaswadji
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.139

Abstract

A study has been conducted to investigate some aspects related to seagrass Enhalus acoroides growth in Barrang Lompo Island Makassar by using a modified tagging method.  Tagging was applied monthly to 20 seagrass stands for seven months with weekly observations. The results showed that Enhalus acoroides growth had a quadratic pattern and was strongly affected by exposed environmental condition, turbidity, nutrient, and temperature.
Pertumbuhan dan Laju Sintasan Krablet Kepiting Bakau (Scyla serrata) dengan Pemberian Jenis Pakan Rucah yang Berbeda Suharyanto Suharyanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 1 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.1.229

Abstract

The aim of this experiment was to find out information on suitable trash fish for the growth and survival rate of mud crabs (Scyla serrata). The research was conducted in Marana Brackishwater pond research station of the Research Institute for Coastal Aquaculture, Maros, South Sulawesi for 28 days. Twelve  aquaria of 60 x 40 x 40 cm3 were used in this experiment using Completely Randomized Design. Treatments tested were four differents species of trash fishes i.e. A; sibula   (Sardinella sp), B; tembang  (Clupea sp), C; Anchovy (Stolephorus sp) and D; Tilapia  (Tilapia mosambica), each with three replications. Twentyfive pieces of crablets  of  5,9 + 0,2 mm in carapace width and 0,06 + 0,02 g in body weigth were stocked in each  aquarium.  Crablets  were then fed with trash fish with the dosage of 15% of total biomass per days  given twice a day. The results showed that the growth of crablets fed with tilapia was the best trash fish (P>0.05), among the treatments which were tested. The use of other fishes were not significantly different  (P>0.05) on the crablet growth.
Keanekaragaman Tumbuhan Pakan Bagi Tapir (Tapirus indicus), Kijang (Muntiacus muncak), Kukang (Nycticebus coucang) dan Kondisi Habitat di Kawasan Gunung Tujuh, Taman Nasional Kerinci Seblat, Jambi W. Rosa Farida; Wirdateti Wirdateti; H. Dahruddin; G. Sumaatmadja
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.163

Abstract

A study on the diversity of feed plants of tapir (Tapirus indicus), barking deer (Muntiacus muncak), and slow loris (Nycticebus coucang) as well as their habitat condition has been conducted at Gunung Tujuh, Kerinci Seblat National Park, Jambi. Survey was carried out by visiting places where the animals are usually found and by taking collection of herbarium plant specimens of those animals’ feed.  The habitat of tapir is in a region of Gunung Tujuh forest up to the altitude of 2,020 m above sea level, while that of barking deer is in Gunung Tujuh forest  where dense bushes at the edges of forest until the altitude of 1620 m above sea level is favored and that of slow loris is in low region of forest. Thirty eight species belonging to 25 families of forest plants as feed resources for tapir, barking deer, and slow loris were found. Amaranthaceae, Anonaceae, Clusiaceae, Moraceae, and Myrsinaceae were recorded as feed plants for tapir, while Euphorbiacea, Fagacea, Lauracea, and Urticacea were for slow loris and Solanaceae was for barking deer.
Keanekaragaman, Persebaran dan Pemanfaatan Jenis-Jenis Anggrek (Orchidaceae) di Resort Citorek, Taman Nasional Gunung Halimun-Salak, Jawa Barat Asep Sadili
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 1 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.1.255

Abstract

Diversity, distribution, and utilization of orchids have been studied on the mountains of Halimun-Salak National Park. Samples were taken from Citorek Resort with focused exploration on Citorek, Lebaktugu, Cinakem, Cibedug, and Ciawitali areas. The total,of 107 species belonging to 46 genera were recorded. As many as 27 species were recorded as javanese endemic species of Indonesian islands. Most species have no economic value and only a few orchids have been cultivated. The Calanthe, Cymbidium, Phaius and Renanthera have been used for cross breeding.
Biosorpsi Krom Total dalam Limbah Cair Batik dengan Biosorben yang Dikemas dalam Kantung Teh Celup Sri Lestari; Sudarmadji Sudarmadji; Shilahuddin Djalal Tandjung; Sri Juari Santosa
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.2.428

Abstract

Krom dalam limbah cair batik dapat dikurangi dengan metode biosorpsi menggunakan biosorben yang murah, melimpah dan mudah didapat yaitu S. cinereum dan limbah baglog P. ostreotus. Kelemahan ukuran partikel yang kecil adalah sulit dipisahkan dari limbah ketika diaplikasikan, sehingga diperlukan sebuah bentuk atau kemasan biosorben yang memudahkan proses aplikasinya yaitu mengemas dalam kantung teh celup. Tujuan penelitian adalah mendapatkan perbandingan komposisi dan ukuran partikel yang optimum menyerap krom total pada limbah cair batik. Penelitian dilakukan secara eksperimental dengan rancangan petak terpisah. Data yang diperoleh dianalisis menggunakan analisis varian dengan tingkat kesalahan 5%. Hasil penelitian menunjukkan persentase adsorpsi tertinggi terdapat pada perbandingan 3:1 μm sebesar 62.69%. Biosorben yang dikemas dalam kantung teh celup efektif menurunkan krom pada limbah cair batik.
Pengembangan Indirect Dipstick ELISA untuk Deteksi Aflatoksin B1 pada Pakan dan Jagung Sri Rachmawati; Prima Mei Widiyanti; Hasim Munawar
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.2.129

Abstract

Enzyme Linked Immunosorbent Assay (ELISA) is an immunological method can be used to analyze aflatoxin B1 (AFB1) in feed. ELISA technique must be done by using an instument (ELISA reader) which is not effective when used in the field. Therefore, the simple ELISA technique is needed such as indirect dipstick ELISA (d-ELISA). The aim of research is to develop AFB1 screening method using   d-ELISA. The research is focusing on  development and validation of indirect d-ELISA, and its application on sample of feed and corn. The results showed that the best coating time for antigen AFB1-BSA (0,4 ug/ml) is 24 hours, reaction time for antibody anti AFB1 (1/800) and AFB1 standard is 15 minutes, and reaction time for goat anti rabbit-HRPO conjugate 1/2500 and substrate of orthodiasianin (ODN) is 20 minutes. The results of indirect d-ELISA on 22 samples are found that 7 samples are contaminated by AFB1 with concentration above 20 ng/g and 7 samples are contaminated by AFB1 with concentration in the range of 0 – 20 ng/g. Finally, it is concluded that the indirect d-ELISA is applicable to be used in the fields.
Keanekaragaman dan Pemanfaatan Ubi-Ubian sebagai Alternatif Tanaman Pangan di Kecamatan Bantarkawung Kabupaten Brebes Uswatun Hasanah; Edy Purwono Hadi; Hexa Apriliana Hidayah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.2.215

Abstract

A research on the diversity and use of tuber as alternative food crops in Bantarkawung, Brebes has been conducted. This research was aimed to find out the kinds of tuber used as alternative food crops.  The method used was survey with a purposive random sampling applied to eighteen villages, The data were obtained by interviewing using questionnaire for each owner. They were analyzed descriptively for tuber type.  The result of this study showed that there are nine species of tuber plant used as alternative food crops: Manihot esculenta, Ipomoea batatas., Dioscorea alata, D. hispida, D. esculenta, Canna edulis, Colocasia esculenta, Amorphophallus campanulatus, Maranta arundinacea, and 7 cultivars from 3 species of plant that were Manihot esculenta.’ Darmo’, M. esculenta ‘Mentega’,  M. Esculenta ‘Valenca’, Ipomoea batatas. ‘Selat’, I. batatas ‘Sriwil’, Colocasia esculenta ‘Udang’ and C. esculenta ‘Pari’.  M. esculenta were more often found while D.  hispida was the less frequently used.   People in Bantarkawung used tuber as the alternative food crops for self consumption by boiling, frying, roasting and making them as meal.  The meal was processed into various processed foods, part of them were sold.
RAPD Profile Of Winged-Bean [Psophocarpus tetragonolobus (L.)DC] Mutated Using Ethyl-Methane Sulphonate Fajri Hartanti; Adi Amurwanto; Agus Hery Susanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 1 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.1.326

Abstract

Genetic diversity of winged-bean [Psophocarpus tetragonolobus (L.) DC] can be produced by induced mutation with EMS (ethyl-methane sulphonate). To identify the genetic diversity after EMS application on winged-bean, molecular analysis using RAPD (Randomly Amplified Polymorphic DNA) technique was carried out. This study aims to identify RAPD profile of winged-bean after EMS application. Winged-bean seeds of both long and short pod types were soaked in wet paper towels for 6 hours followed by soaking in EMS of 0.3% and 0.5% for 4 hours. Winged-bean seeds of both pod types without EMS served as control. Ten of the 30 primers operon technologies USA and 2 primers bangalore genei showed sufficiently clear bands (OPA 1, OPA 3, OPA 10, OPA 15, OPA 19, OPB 1, OPB 6, OPB 7, ES10C27, and ES10C28). All the ten primers produced polymorphic fragments with a total number of 320 and size ranging from 100 bp to 2,000 bp. As many as 66 loci were observed. The polymorphism values obtained ranged from 16.67% to 46.97%. This low polymorphism values indicate that EMS concentrations applied have not resulted in sufficiently high genetic variation. At the same time, the genetic distance observed were only 0.11 to 0.41.