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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 342 Documents
Potensi Reproduksi Ikan Air Tawar Sebagai Baby Fish Nuning Setyaningrum; Eko Setio Wibowo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.2.475

Abstract

Informasi dasar mengenai potensi reproduksi ikan dapat diperoleh dari tinjauan fenomena perkembangan gonad, untuk memprediksi proses reproduksi sampai ikan memijah dan menghasilkan benih sebagai baby fish. Tujuan penelitian adalah mengetahui fekunditas, diameter telur, gonado somato index (GSI) dan kelangsungan hidup larva. Metode yang digunakan survey, pengambilan sampel puposive random. Materi penelitian adalah induk Cyprinus carpio, Barbonymus gonionatus, Osteochillus vittatus, Oreochromis niloticus dan Clarias gariepinus, sebanyak 3 pasang/species. Variabel  adalah fekunditas, diameter telur, gonado somato index (GSI) dan kelangsungan hidup larva. Parameter yang diukur berat ikan, berat gonad, jumlah telur sebagian, ukuran telur dan jumlah larva hidup. Metode penelitian survey, pengambilan data adalah puposive  random dan data dianalisis dengan Anova. Hasil penelitian  diperoleh bahwa  fekunditas dan GSI  pada 5 species ikan menunjukkan perbedaan (P<0,05), B. gonionotus  memiliki fekunditas tertinggi rata-rata 182.320 butir dan O. vittatus memiliki nilai GSI tertinggi  35,13%. Diameter telur  berbeda-beda pada semua  spesies,  B. Gonionotus memiliki diameter terkecil yaitu kisaran 0,486 – 0,729 mm, sedangkan diameter terbesar pada ikan C. carpio rata-rata  1,170 mm. Larva  O. Niloticus menghasilkan prosentase kelangsungan hidup terbaik sebesar 97%, O. vittatus 93%, C. carpio 77,2%, C. gariepinus 51,6%, B. gonionotus 31%  dan uji Least Significant Difference  larva O. niloticus dan O. vittatus  memiliki kelangsungan hidup sama baik,  berpotensi dikembangkan sebagai baby fish. O. vittatus memiliki peluang besar dikembangkan menjadi baby fish sampai ukuran 5 g/ekor dengan fekunditas rata-rata 16.250 butir.
Fekunditas Udang Galah (Macrobrachium rosenbergii ) Betina Pasca Ablasi Unilateral dan Suplementasi Vitamin E pada Pakan Rachmawati Nasution; Moh. Husein Sastranegara; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 3 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.3.135

Abstract

This study reports on giant freshwater prawn fecundity with or without unilateral ablation treatment combined with alpha tocopherol (vit E) during 3 month culture. This exsperimental study applied 8 combination treatments of 4 different vit E dosages, i.e. 0, 200, 400, and 600 IU per kg feed with or without unilateral ablation. The results showed that treatments very highly significantly different (p<0,01) in fecundity with the 200 IU vit E dose combined with unilateral ablation was the best (45.228 ± 3.867 egg) optimum resulted in fecundity of the treated females. The overall fecundities averages were ablation group with 0 IU = 41.835 ± 837 eggs; 200 IU = 45.228 ± 3.867 eggs; 400 IU = 43394 ± 1523 eggs ; and the 600 IU = 43.718 ± 2.255 eggs, and for the non ablation with vit E group were 0 IU = 45.293 ± 2.899 eggs, 200 IU = 44.106 ± 1.759 eggs, 400 IU = 46.623 ± 2.556 eggs, and 600 IU=51.824 ± 1.132 eggs. The analysis results also proven that ablation factor gave very highly significantly (p<0,01) and vit E factor separately and in combination with ablation significantly (p<0,05) determined the females fecundity. In conclution, combination of unilateral ablation and tocopherol effective improved fecundity and the combination of unilateral ablation and vit E 200 IU the optimum resulted in giant freshwater prawn females fecundity.
Longevitas dan Efikasi Pelet Trichoderma harzianum terhadap Sclerotium rolfsii Penyebab Penyakit Layu pada Tanaman Tomat (Solanum lycopersicum L.) Juni Safitri Muljowati; Uki Dwiputranto; Iman Budisantoso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.1.221

Abstract

Trichoderma harzianum is one of the antagonist fungi that can be used as a biofungicide to control soil-borne pathogens such as Sclerotium rolfsii, the cause of wilt isease of tomato. To ease the application of T.harzianum fungi in the field, it should be prepared a formulation in form of pellet. The longevity of T.harzianum pellet is determined by the viability of T.harzianum fungi contained in it, whereas the efication/ effectivity of T.harzianum pellet is determined by the longevity of the pellet.  The objectives of this study are to investigate the interaction between storage period and application dosages in controlling wilt disease of tomato;  and the dosage and storage period of T.harzianum on the effectiveness in controlling wilt disease of tomato. The experiment was carried out experimentally by using a Completely Randomized Design (CRD) in a factorial pattern. The first factors were storage period (W) consisted of 5 levels, i.e. 0, 3, 6, 9, and 12 weeks; the second factors were theapplication dosages (D) consisted of 6 levels, i.e. 0, 25, 50, 75, 100, and 125 grams. They were replicated 3 times each. The viability observation of T.harzianum was completed at the end of storage period of the pellets, the disease occurence and severity was since the 4th day after inoculation with 4 day interval until the tomato plant produced flowers. Research result showed that the interaction between storage period and different application dosages of T.harzianum pellets has influenced the increase of wilt disease control of tomato. Furthermore, storage period of 6 weeks and T.harzianum application of 50 g were the most effective in controlling wilt disease of tomato.
Kelimpahan dan Potensi Biofuel Mikrofitobenthos dari Perairan Sungai Pekacangan yang terkena Limbah Cair Tapioka Christiani Christiani; A Ilalqisny Insan; Dwi Sunu Widyartini
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.340

Abstract

Microphytobenthic a benthic plants, found in the waters of the sunlight reaching the bottom waters. In addition to acting as a primary producer also has the potential to produce renewable biofuels  The purpose of this study was to determine the microphyitobenthic abundance of river Pekacangan affected tapioca liquid industrial waste and to estimate its potential as a biofuels. This research used survey method with the sampling technique is purposive sampling based on existing environmental setting. As the main parameters are the types of microphytobenthic, while supporting research parameters are temperature, light penetration, pH, dissolved O2, BOD5, current velocity, TSS, TDS, nitrate, orthophosphate and silica. Microphytobenthic obtained at stations affected by the waste, in screening to determine its oil content.Abundance of microphytobenthic at a station directly exposed tapioca liquid waste 620 ind.mm-2.Isolated microphytobenthic River Pekacangan affected tapioca liquid waste had 22 isolates of microphytobenthos and after screened allegedly 7 isolates potential as a biofuel, namely Navicula sp., Amphora libyca, Ulothrix sp., Anabaena sp., Planctonema sp., Cymbella sp., and Eunotia sp.
Konstruksi Mutan Pseudomonas sp. untuk Meningkatkan Produksi Indole Acetic Acid (IAA) melalui Mutagenesis dengan Transposon Aris Tri Wahyudi; Mutiha Panjaitan; Nisa Rachmania
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.596 KB) | DOI: 10.20884/1.mib.2009.26.3.158

Abstract

Pseudomonas sp. is one of bacterial groups having ability to promote plant growth and health. Of a hundred successfully isolated Pseudomonas sp. from soybean rhyzosphere, 98 were found to produce indole acetic acid (IAA) ranging from 0.33 to 16.02 ppm. These isolates are bacilli, motile, Gram negative, and showing positive oxidase assay. One of them, i.e. Pseudomonas sp. CRB17, can promote plant growth by means of significant stimulation of primary root length and lateral root number. This isolate was then subject to mutagenesis using transposon Mini-Tn5Km1 to increase IAA production. Mutagenesis was done by conjugation between E coli S17-1 (l pir) carrying transposon mini-Tn5Km1 (donor) and Pseudomonas sp. (recipient), resulting in conjugation frequency of approximately 3.1 x 10-5 cell per recipient. The resulted CRB17 mutants were then tested for their ability to produce IAA, one of which showed an increment of IAA production up to 77.5%, while some others showed no significant change or even had a reduction to 55.3%.  Sequence analyisis of 16S rRNA gene of Pseudomonas sp. CRB17 indicated that it has a high homology with that of Pseudomonas plecoglossicida (identical value of 99%). The results recommends that mutagenesis using transposon can be applied to increase IAA production, especially in Pseudomonas sp. CRB17.
Paleovegetasi Berdasarkan Bukti Palinologi Kala Pliosen Cekungan Banyumas Kartika Anggi Hapsari; Sukarsa Sukarsa
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 3 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.3.251

Abstract

Taxonomy is a branch of biology which is supported by palynological evidence. Palynology concerns to pollen and spore. The purposes of this research were to figure out plant diversity of Pliocene based on fossil pollens and to reconstruct vegetation and climates of Pliocene based on plant diversity. A survey has been conducted in Bungkanel, Purbalingga. Sampling was done for 25 sediment samples and then they were crushed, carbonate-, sulfide-, fluoride-, and organic-separated, centrifuged, oxidizing, alkali water-bathed, and mounted. Pollen and spore were identified in the laboratory with a binocular microscope. Data were analyzed descriptively, clustering, biostratigraphic Ranging-Zone, AP-NAP-Spore and PMI analysis has been done to determine the taxon, sediment age, paleo-diversity, paleo-vegetation and paleo-climate. The result showed that there were sixteen genera and three species of terrestrial palynomorph; two phyla, one class and one order of marine palynomorph. The Age of Pliocene was indicated by the appearance of Podocarpus imbricatus and Stenochlaena laurifolia (Stenochlaenidites papuanus).  The results of clustering analysis showed that there were six groups of samples with Shannon-Wienner H’ diversity index of <2. However, the palinological diagram showed that there were three local zones of vegetation-alteration. In addition, AP/NAP/Spores and PMI indicated that there were six zones of climate change.
Gambaran Populasi Golongan Darah Subgroup A (A1, A2) di PMI Kulon Progo Hieronymus Rayi Prasetya; Bambang Heru Budianto; Hernayanti Hernayanti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.1.375

Abstract

Subgroup A1 and A2 are the most important in the blood group A. Subgroup A1 has the A antigen more than A2 subgroup, the A2 subgroup can cause misidentification of blood group due to poor A antigen and genetic variation possessed. Misidentification of the blood group will increase the risk of transfusion reactions. This research aims to describe the A1 and A2 subgroup population in Kulon Progo district. This study was conducted with a cross sectional sampling technique. The sample in this study were taken from donors of blood group A in Kulon Progo Red Cross. Identification of A1 and A2 subgroup is done by using lectin (Dolichos biflorus extract). The result of the examination of 53 samples showed that 96,2% was A1 subgroup and 3,8% was A2 subgroup.Key words : Subgroup A1, Subgroup A2, Population, Kulon Progo
Aktivitas Amilase dan Laju Metabolisme Ikan Lele Dumbo (Clarias gariepinus) pada Kondisi Puasa dan Pemberian Pakan Kembali Esti Dwi Pratiwi; Untung Susilo; Slamet Priyanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.123

Abstract

The study to determine amylase activity and metabolic rate of catfish, Clarias gariepinus under starvation and refeeding conditions has been conducted with three treatments and four replications in randomized completely design. The result showed that the amylase activity was significant different (P<.05) only at second week after treatment  and metabolic rate was significant different (P<.050 at first and second week after treatment. Conclutions, the amylase activity and metabolic rate of catfish have been decreased at starvation, especially at second week, but it recovery at refeeding condition.
Kajian Tingkat Bahaya Erosi Dan Indeks Nilai Penting Di Hutan Rakyat Di Desa Candiwulan Kecamatan Kutasari Kabupaten Purbalingga Gunanto Eko Saputro; Moh. Husein Sastranegara
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.3.210

Abstract

This study was aimed at assessing the danger level of erosion, vegetation conditions, and the relationship between the level of erosion and vegetation in the community forest in Candiwulan Village, District Kutasari, Purbalingga. The results showed that the rate of erosion in forest areas studied was at 56.62 or go to class II or mild (erosion rate between 15-60 tonnes / ha / year), plants that are found in forest areas quite diverse.  There are12 plant seedling species, 14 species of saplings plants, 11 species of pole plants and 12 species of trees.  While in zone II there were only 9 plant species.  The calculation included the relative density, relative frequency and relative dominance to known important value index (IVI) to determine the role of each of the plants in both zones. The dominant role of plants at the seedling stage is coffee with IVI 70.49, coffee saplings showed INP 51.77,  the teak poles with IVI 76.69 and tree of Albizia with IVI 108.79. The vegetation diversity in the area of community forest Candiwulan Village has caused mild erosion at hazard level. The cultivating patterns and the type of crops cultivated influence the erosion and run off because it affects the land and the soil cover and the production of organic material that serves as a ground cover.
Kandungan Alginat Sargassum polycystum pada Metode Budidaya dan Umur Tanam berbeda Dwi Sunu Widyartini; A. Ilalqisny Insan; Sulistyani Sulistyani
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 2 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.2.303

Abstract

Sargassum polycystum is one of seaweed what be able to produce alginate. Cultivation development intensively use common cultivation  methods and planting age of growth season properly which can be able to produce good growth and highest alginate. This study is used an experimental method. The basic design used for the content of alginate S. polycystum use completely randomized design (CRD) with Split Plot Design which repeated 3 times. The main plot of this study is a method of cultivation are included by floatting method and bottom method and Subplot is age after planting are consist of 7, 14, 21 and 28 days. The cultivation result of S. polycystum by bottom method with 28 days, showed the highest growth rate about 17.38 g.day-1, while the lowest growth were founded on bottom method on age 7 days about 3.42 g.day-1. The Results of analysis of variance showed that the interaction of cultivation method and planting age have significant effect on the subtances of alginate from S. polycystum. The highest alginate are produced by bottom method of planting age on 28 days about 58.33%. The Lowest alginate are produced by floating and bottom methods of planting age on 7 days about 11.67%. Quality of alginate farmed bottom methods of color is more yellowish than the cultivation of the floating method.