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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
Xanton dari Fraksi Aktif Antioksidan Kulit Batang Kandis Gajah (Garcinia griffithii T. Anders) Elfita Elfita; Supriyatna Supriyatna; Huesin H Bahti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 1 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.1.151

Abstract

Garcinia is a large genus of polygamous trees or shrubs, distributed in the tropical Asia, Africa, and Polynesia, and is a rich source of bioactive molecules including xanthones, flavonoids, benzophenones, lactones, and phenolic acid. In the present study, an attempt was made to evaluate the antioxidant activity from stem bark of G. griffithii using radical scavenging 1,1-diphenyl-2-picryl hydrazyl (DPPH) model systems. Xanthone from antioxidant active fraction had been isolated and this compound had been identified by the use of ultraviolet (UV), infrared (IR), and proton nuclear magnetic resonance (1H-NMR) spectroscopy.
Fauna Krustasea di Perairan Kepulauan Anambas, Propinsi Kepulauan Riau Ernawati Widyastuti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 1 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.1.149

Abstract

Observation of crustacean fauna in Anambas Islands waters was conducted from 20 May to 4 June 2002. The selected locations were Ranap Island, Matak Island, Pemutus Island, Akar Island, and Jemaja Island. The aim of this study was to know the diversity and evenness of the crustacean species. Sampling was conducted by applying quadrant transect and other collections. Forty one species of crustaceans, representing 14 families, were collected from those selected locations including 23 species that were collected from transect area. The results showed that diversity index of the crustacean fauna was 0.65 to 0.98 and species evenness was 0.85 to 0.98. Based on the species diversity index, all those selected locations could be said as having low diversity.
Struktur, Komposisi serta Potensi Tumbuhan Bawah sebagai Tumbuhan Obat di Hutan Heterogen di Desa Cikakak Kecamatan Wangon Kabupaten Banyumas Sulistyani Sulistyani; Erie Kolya Nasution; Hexa Apriliana Hidayah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 2 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.2.239

Abstract

A research entitled “The structure, Composition and Potency of Ground Vegetation as Medical Plants in Heterogenous Forest at Cikakak Village Wangon Subdistrict banyumas Regency” aims to find out the structure and composition of ground vegetation species and ground vegetation that have a potency of medicalplants. Material and tools usud in this reseach were ground vegetation  is sublimat, GPS, altimeter, thermohygrometer,  soiltester, luxmeter, plastic bags, rollmeter, sasak. The research were used survei methode and square plots quadrate methode used a sampling method. The result of the observation showed that ground vegetation composition of heterogenous forets at Cikakak village Wangon subdistrict Banyumas regency was builled from 72 species belonging to 33 families  and  order from the most to the least abundance habitus were follow herb 49 species ,shrub 11 species, seedling 8 species, and liana 3 species. The result of the reseach ground vegetation potency as big enough medical plants ist 65,27 %.
Ekspresi mRNA LMP2A Epstein-Barr Virus dari Biopsi Jaringan dalam Blok Parafin Berpotensi sebagai Biomarka dalam Diagnosis Karsinoma Nasofaring Roni Afriansya; Hendro Pramono; Hidayat Sulistyo; Daniel Joko Wahyono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.323

Abstract

Karsinoma nasofaring (KNF) merupakan tumor epitel yang terletak di nasofaring dan merupakan penyakit genetik multifaktor yang endemik. Penyebab utama KNF adalah infeksi oleh Virus Epstein-Barr (EBV). Keberadaan EBV pada penderita KNF dapat diketahui dengan ditemukannya DNA EBV dalam spesimen biopsi jaringan penderita KNF. Genom EBV dan ekspresi sebagian dari produk gen laten virus secara konsisten terdeteksi hampir di setiap sel dalam kanker ini, salah satunya adalah gen Latent Membrant Protein (LMP). Aktivitas mRNA EBV lebih mencerminkan patogenesis KNF yang  sesungguhnya dari pada diagnosis serologi dan pengukuran DNA EBV di sirkulasi. Penelitian dilakukan di Laboratorium PA RSUD Margono Seokarjo, Purwokerto/Lab PA Fakultas Kedokteran dan laboratorium genetika/molekuler Universitas Jenderal Soedirman Purwokerto. Analisis Ekspresi mRNA LMP2A EBV dengan teknik one step RT-PCR dan produk RT-PCR (amplikon cDNA) divisualisasi dengan elektroforesis gel agarosa 1%. Hasil ekspresi mRNA LMP2A EBV adalah 27,3% (6 dari 22 sampel). Kesimpulan, metode one step RT-PCR dapat digunakan untuk menganalisis ekspresi mRNA LMP2A EBV dari sampel biopsi jaringan KNF dalam blok paraffin dan hasil positivitas ekspresi mRNA LMP2A EBV sedang, sehingga berpotensi digunakan sebagai petanda biologi molekul diagnosis KNF.
Pemakaian Sel Hela dalam Uji Sitotoksisitas Fraksi Ethanol Biji Mimba (Azadirachta indica) Ira Djajanegara; Priyo Wahyudi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.112

Abstract

Distribusi Foraminifera Bentik Resen di Perairan Lombok (Suatu Tinjauan di Daerah Gili Air, Gili Meno dan Gili Trawangan) Suhartati M Natsir
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 2 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.2.198

Abstract

Study on distribution of benthic foraminifera in Gili Air, Gili Meno and Gili Trawangan,Lombokwas held on February 12 – 15, 2008. Samples were collected respectively in the each side, including Northern, Eastern, Southern and Western part of Gili by using Van Veen Grab. Every side of the islands was collected three samples within 10 meters of clearance of each location. Thus, the collected samples of the three Gili were totally on 36 samples. The ecological parameters were also being measured, i.e. depth, temperature, salinity, pH and turbidity. The number of collected specimens of foraminifera was totally 46197 and the most specimen belongs to Suborder Rotaliina (423026 specimens). Then, 2137 specimens were recognized under Suborder Milioliina and the others 1036 specimens belong to Suborder Textulariina. The collected specimens consist of 49 species of 29 genera, which composed from Suborder Rotaliina (25 species in 20 genera), Suborder Milioliina (20 species, 7 genera) and Suborder Textulariina (4 species 2 genera). 
Variasi Morfologi Bambu Tali [Gigantochloa apus (Schult.F.) Kurz.] pada berbagai ketinggian tempat di Sub Daerah Aliran Sungai Pelus Tria Fauzi Prabandani Hakim; Pudji Widodo; Eming Sudiana
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.294

Abstract

A study on the morphological variation of tali bamboo [Gigantochloa apus (Schult.f.) Kurz] has been conducted at various altitudes of Sub Watershed Pelus Banyumas, Central Java. This study was aimed at determining the morphological variations in relation to altitudes above sea levels. The method used was survey with stratified sampling.  The variables measured included culm diameter, culm internode length, culm sheath length and width, ovate-triangular blade length and width, leaf blade length and width, stem color and hairs, and altitude. The results of this study indicated that the altitudinal difference effect some morphological characteristics of tali bamboos such as: 1) green young seedling with black hairs found at 200-299 m and 300-399 m asl. 2) morphological variations are effected by altitude. Tali bamboo grows optimally at the altitude of 400 to 499 m asl. The results of regression analysis showed that the altitude has a relatively close correlation with somes of morphological character of tali bamboo.
Biodisel dari Mangrove Jenis Nyamplung (Callophylum inophyllum) sebagai Alternatif Pengganti Bahan Bakar Minyak Fosil Suyono Suyono; Ninik Umi Hartanti; Agus Wibowo; Narto Narto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.505

Abstract

Resources of fossil fuel continue to decrease and cannot be renewed so that replacement alternative is needed. Biofuels (biodiesel) become one of the alternatives because it can be renewed and environmentally friendly. First-generation biodiesel from food sources and second generation of food waste can disrupt the availability of food. Those are inefficient because it requires extensive land for its provider. Seed of nyamplung (Callophylum inophyllum) is very potential as biodiesel raw material because of its oil content 40-70% is high compared to other types of plants, such as jatropha range of 40-60% and palm of 46-54%, raw materials saving and having twice of fuel as much as kerosene. The general purpose of this research is to produce third generation biodiesel from nyamplung  as material to overcome fossil fuel crisis especially for fishermen and farmers in coastal area of Brebes Regency and surrounding areas. To be able to achieve the goal / general target is done by assessing oil content in nyamplung seed, biodiesel synthesis, intensive culture techniques and models of development of mass culture. The biodiesel yield produced from nyamplung seed oil in this study was 82.87%, in the range of biodiesel yields of previous researchers through a single stage transesterification of 79.03-91,00%. Cetana number and alkyl ester figures in the resulting biodiesel respectively 79.5 and 99.71% have met the Indonesian National Standard (SNI). The resulting density was 893.1 kg / m3, fog point  was 20oC and total glycerol  was 0.26%, was approaching the SNI by an insignificant difference, while the acid number generated was 1.86 mg KOH/gr of biodiesel and the biodiesel viscosity was 15.7 mm2 /s has not fulfilled the SNI significantly. To improve the number of acids and viscosity is still needed refinement biodiesel synthesis process through the diversification of the number of esterification stages, alternative types and catalytic levels or optimization of transesterification reaction temperature.
Potensi Jamur Mikoriza Arbuskular dalam Mengendalikan Penyakit Busuk Pelepah pada Tanaman Jagung Soenartiningsih Soenartiningsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 1 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.1.232

Abstract

Sheath blight disease is important disease on maize, it caused by Rhizoctonia solani. biological control using arbuscular mycorrhizal is assumed can reduce expansion of the soil borne disease. The experiment was carried out using arranged in completely randomized design of factorial, with different density spore and two isolate of AM fungus ie: Glomus sp and Acaulospora mellea. There were three replicates arbuscular mycorrhizal at 100-300 spore per 10-30 grams propagule was inoculated to each planting hole and inoculated R. solani fungi 30 days after planting. Sheath blight disease observation was taken 10-50 days after inoculation R. solani, while observation their  histology stalk and root at 50 days after inoculation of R. solani. The result of the experiment that inoculation  of AM fungus with 100-300 spore have potential to control sheath blight disease on maize. At the plant infected by AM fungi isolate of Glomus sp its emphasis reach of 21,9%–40,98%, while infected by A. mellea emphasis reach 21,48%–39,93%. The damage of stalk tissue at the plant infected by R. solani was more acute than at the plant infected by both R. solani and mycorrhizal fungus even they had the same symptom, that was an necrosis at their stalk tissue. The plant infected by R. solani and mycorrhizal fungus were not different with the root tissue of healthy plant.
Aktivitas Protease dan Komposisi Proksimat Tubuh Ikan Sidat (Anguilla bicolor Mcclelland) pada Kondisi Puasa dan Pemberian Pakan Kembali Untung Susilo; Nuning Setyaningrum; Farida Nur Rachmawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.2.133

Abstract

Protease activity and body proximate composition of eel, Anguilla bicolor McClelland., under starvation and refeeding conditions with three treatments and four replications in randomized completely design has been evaluated. The result showed that the protease activity of eel was no significant different (P>.05) between treatment, but body proximate composition has significant differences (P<.05), especially in protein and lipid contents at starvation condition. It can be concluded that the eel protease activity did not change under starvation and refeeding condition, but the lipid content decreased at starvation condition.