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INDONESIA
Jurnal Ilmu Alam dan Lingkungan
Published by Universitas Hasanuddin
ISSN : 20864604     EISSN : 25498819     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Veterinary
Jurnal yang memuat hasil - hasil penelitian terkait ilmu alam dan lingkungan termasuk review meliputi lingkungan, kelautan, konservasi, mikrobiologi, bioaktif, dan yang relevan.
Arjuna Subject : -
Articles 159 Documents
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Mikropropagasi Talas Satoimo Colocasia esculenta (L.) Schott var. Antiquorum melalui Meristem Apikal Mustika Tuwo; Elis Tambaru; Baharuddin Patandjengi
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13093

Abstract

Japanese taro (satoimo) is a functional food source belonging to the Araceae family. Satoimo seedling production is conventionally constrained by limited land and uncertain climate, so that production decreases. One of the efforts to increase the production of satoimo seedlings can be done through plant tissue culture (in vitro). Meristem culture is an in vitro culture technique that is capable of producing plants free of viruses, bacteria and fungi. MS medium is a plant basal medium still requires the addition of growth regulators for growth and development of explants. This study aims to obtain the optimum concentration of growth regulator BAP (6-benzylaminopurin) which is able to produce the best taro growth in the apical meristem culture of satoimo taro. Explant sterilization is carried out at the tip of the shoot that emerges from the tuber. Then washed with detergent under running tap water for ± 30 minutes, followed by sterilization in Laminar Air Flow (LAF). Shoot tip explants were sterilized with 5.25% sodium hypochlorite plus 3 drops of tween 20. Soaking time varied depending on the treatment and then planted on MS medium with various concentrations of BAP. The parameters observed were the percentage of contamination, survival rate of explants, percentage of explant mortality, number of shoots, number of leaves and shoot height. Observations were made for 8 weeks. The mean and standard deviation were calculated using Microsoft Office Excel computer software. The results showed that the BAP concentration of 0.5 ppm was the best concentration for shoot growth of shoots and leaves, while the BAP concentration of 1 ppm was the best concentration for shoot height in the satoimo taro plant.
Uji Daya Hambat Ekstrak Etanol Daun Tapak Kuda Ipomoea pes-caprae Terhadap Pertumbuhan Bakteri Propionibacterium acne Etrin Z. E. Saputra Linggar; Eka Astuty; Yuniasih M.J. Taihuttu
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13094

Abstract

Ipomoea pes-caprae is a plant that has the ability as an anti-bacterial. The active compounds of Tapak Kuda leaves as anti-bacteria are flavonoids, alkaloids, and tannins. The purpose of this study was to determine the ability of the ethanol extract of tapak kuda leaves to inhibit the growth of Propionibacterium acne bacteria. This research is a laboratory experimental study using the disc diffusion method and using various extract concentrations of 5%, 10%, 25%, 50%, and 100%. The ethanol extract of tapak Kuda leaves was obtained from the maceration process of tapak Kuda leaves with ethanol solvent, then tested the inhibition of Propionibacterium acne. The parameters observed were the clear zone formed on the growth of Propionibacterium acne bacteria. The results showed that the ethanol extract of tapak Kuda leaves had a low ability to inhibit the growth of Propionibacterium acne bacteria. The clear zone formed at a concentration of 100% was only 1.14 mm while at a concentration of 5%, 10%, 25% and 50% there was no clear zone formed.
Efektivitas Ekstrak Daun Eceng Gondok Eichornia crassipes Sebagai Bahan Antikanker pada Sel Tumor MCF-7 dengan Metode In Vitro dan In Silico Eva Johannes; Andi Ilham Latunra; Mustika Tuwo; Sry Sukmawaty
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13095

Abstract

The use of water hyacinth Eichhornia crassipes bioactive compounds has been developed, including as an anti-cancer base material. This study aims to examine the potential of water hyacinth leaf extract on MCF-7 tumor cells with the MTT method and the affinity of the water hyacinth leaf compound using molecular docking methods. The results showed the effect of water hyacinth leaf extract on MCF-7 cancer cell death due to the active compounds stigmasterol and phytol, while the docking results showed the best binding affinity value on stigmasterol and its receptor 1QR1 which was -9.4.
Analisis Kandungan Kafein Kopi (Coffea arabica) Pada Tingkat Kematangan Berbeda Menggunakan Spektrofotometer UV-VIS Andi Ilham Latunra; Eva Johannes; Besse Mulihardianti; Ophirtus Sumule
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13096

Abstract

A study recently conducted with the title Analysis of caffeine content in arabica coffee (Coffea arabica) based on its maturity level by using Uv-Vis Spectrophotometer. This study aimed to determine the levels of caffeine in Arabica coffee based on its level of maturity, either young, medium and old. Moreover, this study aimed to measure the highest and lowest caffeine levels of Arabica coffe from. Retrieval of young coffee fruit is characterized by green skin fruit, half-old / medium coffee, yellow to orange skin fruit and dark coffee with dark-red skin fruit. The Fruit of Arabica coffee is processed starting from drying, roasting and grinding into coffee grounds. Determination of caffeine content using 2 methods, namely a qualitative test with the parry method and a quantitative test using a UV- Vis spectrophotometer. The results obtained in the qualitative test with the Parry method showed that all three coffee powder samples contained caffeine, this was indicated by a change in color into green. Quantitative test results using UV-Vis spectrophotometer obtained the results of each gram of coffee containing caffeine namely young arabica coffee 11.15 mg or 1.151%, half old / medium arabica 12.85 mg or 1.285 % and old arabica coffee 12.01 mg or 1.201 %. The highest level of caffeine is found in the level of maturity of half-old / moderate coffee and the lowest level of caffeine is found in Arabica coffee with a young age of maturity.
Polimorfisme Gen Glutamylcysteine Ligase Catalytic (GCLC) Sebagai Deteksi Kerentanan Pasien Tuberkulosis Terhadap Stres Oksidatif Akibat Infeksi Mycobacterium tuberculosis Andi Zulkifli AS; Muh. Nasrum Massi; Rosana Agus
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13097

Abstract

The important genetic factor about susceptibility of oxidative stress in Pulmonary Tuberculosis (PTB) has not been determined. The aim of this study was to analyse the association between polymorphism glutamatecysteine ligase catalytic subunit (GCLC) genes with susceptibility of oxidative stress in PTB in Makassar population of Indonesian. The Methods use is a case-control study was performed on 25 paired subjects with or without PTB, These subjects were selected from result of BTA stain from sputum subjects. The following polymorphisms were genotyped by PCR-RFLP of −129C/T in the GCLC gene. Genotype frequencies and allelic frequencies were analysed. The Results was found There were significant differences in the distribution of genotype frequencies for polymorphism −129C/T in GCLC gene between PTB and non-PTB subjects. The distribution of the allelic frequencies of these genes also showed significant difference between the two groups. Conclusions is The genetic polymorphisms in GCLC −129C/T are associated with susceptibility of Oxidative stress on PTP subject.
Analisis Fitokimia Umbi Talas Jepang Colocasia esculentai L. (Schott) var. antiquorum dan Talas Kimpul Xanthosoma sagittifolium L. (Schott) dari Dataran Rendah A Masniawati; Eva Johannes; Winda Winarti
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 2 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i2.17568

Abstract

This research aims to determine the phytochemical compounds of the Japanese taro tuber Colocasia esculenta L. (Schott) var. antiqourum and local taro tubers Kimpul Xanthosoma sagittifolium L. (Schott). This research was conducted in June-August 2020 at the Pharmacy Laboratory, Faculty of Pharmacy, Hasanuddin University, Makassar. This research was carried out from sample preparation, extraction, identification with Mayer, and Dragendroff's reagent, then identified by Thin Layer Chromatography (TLC). Data analysis is presented descriptively by looking at changes in color and shape of the tested liquid. The results of this research indicate that the extract of the Japanese taro tuber Colocasia esculenta L. (Schott) var. antiqourum and local taro tubers Kimpul Xanthosoma sagittifolium contains bioactive alkaloids, flavonoids, steroids / triterpenoids, tannins and saponins.
Pemantauan dan Analisis Tingkat Pencemaran Kualitas Air Sungai di Kabupaten Tebo Diana Sari; Nedy Yahya Nurhadi; Khairul Anwar; Muhammad Isa; Sri Handayani; Sardeni Sardeni
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 2 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i2.17571

Abstract

Tebo regency has a very potential river as surface water to support human life. With the population growth in Tebo Tengah Districts and the development of its utilization, there is a tendency for changes occurred to the condition and quality of river. Density of the population can affect river environmental quality. This is happened because of awareness of the society in how to maintaining a healthy and clean environment. Estimation of water pollution can be done by looking at the influence of pollutants on the life of aquatic organisms and their environment. The units for estimating the presence of these pollutants are classified into biological, physical, and chemical parameters. In determining water quality, these parameters should not stand alone but it can show parameter itself and each of this has value which represents what is called the Water Quality Index. The purpose of this article is to know how the river water quality in Tebo Tengah Districts. This research used quantitative descriptive method. Quantitative method is utilized to determining the status quality of the water with vilification index method. Fecal, TSS, BOD5, DO and Total Phosphate values that exceed the quality standard indicate contamination from domestic and industrial wastewater. In addition, the occurrence of sedimentation due to land conversion causes the TSS value to exceed the quality standard. The calculation results for IKA value show that rivers in Tebo regency is in the lightly polluted category. That thing is because the behavior of the people who still throw garbage into the river environment, and the unavailability of public IPAL infrastructure to treat domestic waste water from residents' houses causes rivers in the Tebo Tengah Districts is still polluted.
Fitoremediasi Logam Berat Cr Menggunakan Eceng Gondok Eichornia crassipes pada Buangan Limbah Cair Tambang PT. Bukit Makmur Istindo Nikeltama (Bumanik) Dan Try Borrong; Fahruddin Fahruddin; Anwar Daud
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 2 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i2.17572

Abstract

The aim of the study is to determine the effect of water hyacinth in absorbing the content of heavy metal in mine liquid waste of Bukit Makmur Istindo Nikeltama. This type of research was an experiment using quantitative descriptive analysis in the from of field data collection, laboratory data processing, and direct observation of phytoremediation process of water hyacinth with several variations in the number of plants including 15,10,5 plants and control tubs observed for 12 days. The variables observed in this study were Cr, BOD, COD, pH and TDS. The results showed that water hyacinth is effective to reduce mine liquid waste. The value of each variable obtained on the 12th day is still under the quality standard that has been set. In treatment I, the addition of 15 water hyacinth plants produces concentration values of Cr :
Isolation of Indigenous Bacteria from Paddy Field for Methomyl Degradation W Wartono; RA Suwignyo; A Napoleon; Suheryanto Suheryanto
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 2 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i2.17579

Abstract

Methomyl is an active ingredient of carbamate group pesticide. The uncontrolled application of methomyl may contaminate the water and soil. The objective of this research was to find indigenous bacteria that could degrade the methomyl. The soil samples were taken from the soil of the rice field located in Musi Rawas District, South Sumatera, Indonesia. The soil bacteria that were found to degrade methomyl were isolated by using a medium containing methomyl. There were 2 of 16 isolates that could grow in a high concentration of methomyl and they were Acinetobacter baumannii and Bacillus megaterium.
Uji Potensi Getah Pepaya Carica papaya Terhadap Kecepatan Penyembuhan Luka Bakar Kulit Tikus Rattus novergicus Arifah Zakaria; Andi Evi Erviani; Eddy Soekendarsi
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 2 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i2.17581

Abstract

Tanaman pepaya adalah salah satu tanaman tradisional indonesia yang berpotensi menjadi agen dalam penyembuhan luka bakar. Kandungan senyawa yang terdapat pada getah pepaya mampu mempercepat penyembuhan luka bakar karena mengandung enzim papain. Enzim papain mampu mencerna jaringannekrotik karena mempunyai aktivitas katalitik dengan mencairkan eschar atau keropeng sehingga memudahkan migrasi sel dari tepi luka ke daerah luka. Tujuan penelitian ini untuk mengetahui efektivitas getah tanaman papaya Carica papaya L. sebagai penutup luka bakar. Penelitian yang dilakukan kali ini dengan melakukan eksperimental laboratorium yang terdiri dari 4 kelompok perlakuan yaitu kontrol positif (gentamicin), kontrol negatif (tanpa Perlakuan), salep campuran getah pepaya dan gentamicin, dan getah pepaya murni. Parameter yang diamati pada penelitian ini yaitu pengukuran luas luka bakar dan presentase penyembuhan luka bakar. Hasil penelitian ini menunjukkan bahwa perlakuan dengan menggunakan getah pepaya murni mampu mempercepat penyembuhan luka bakar derajat II A pada Rattus novergicus yang menunjukkan hasil terbaik dengan persentase penyembuhan luka bakar 96.5% pada hari kelima belas.

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