Biosaintifika: Journal of Biology & Biology Education
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
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<![CDATA[Various Plants of Traditional Rituals: Ethnobotanical Research Among The Baduy Community ]]>
<![CDATA[Iskandar, Johan]]>;
<![CDATA[Iskandar, Budiawati S.]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.8117
<![CDATA[On the basis of recent studies in many places of developing countries it has been revealed that the practices of conservation of biodiversity has strongly determined by traditional ecological knowledge, and beliefs or cosmos. The aim of the study namely to elucidate; (1) some traditional rituals in the swidden management system of the Baduy community; (2) various plants that have been used for performing some rituals in the swidden management system of the Baduy community; and (3) some functions of various plants that have been used in the rituals of the swidden management system of the Baduy community. A qualitative method with ethnobotanical approach was applied in this study. The result of study shows that 9 kinds of the traditional rituals that have been predominantly undertaken by the Baduy community in the management of swidden farming system. At least 50 plant species representing 28 families have been used for those performing traditional rituals. The main function of plants in the rituals is considered as the symbolic meaning and rational function. The result of study has been considered very important that the traditional ecological knowledge and beliefs must be considered to conserve biological diversity. ]]>
<![CDATA[Resistance Inheritance of Plutellaxylostella Population to Residual of Emamectin Benzoat ]]>
<![CDATA[Tarwotjo, Udi]]>;
<![CDATA[Rahardian, Rully]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.8048
<![CDATA[Excessive use of insecticides drives the increasing ability of pests to become resistant. The objectives of this research were to study the susceptibility and the resistance inheritance of the eleven population of P. xylostella to emamectin benzoate. The leaf-dip bioassay was applied to determine the sensitivity of P. xylostella to emamectin benzoate. The offspring of backcrossed F2 were tested whether the resistance was controlled by monogenic. The results showed that the LC50 of the Selo population was 53.42 ppb, and the Puasan population was 212.13 ppb. The genetic analysis showed that the backcrosseddegree of dominance (D) was less than 1. It was indicated that the P. xylostella resistance to emamectin benzoate was recessive. The value of LC50 of the backcrossed F1? x ?S (177.99 ppb) and its reciprocals x ?R (F1) (201.69 ppb) were not significantly different with the value of LC50 resistance population. This suggests that the nature of P. xylostella resistance to emamectin benzoate was controlled by monogenic.The result of the study would be beneficial for developing strategy to maintain susceptible population using refugee plant during lack of their host.]]>
<![CDATA[Cigarette Smoke Induces Colorectal Carcinogenesis in Wistar Rats by Decreasing The Expression of APC, MSH2 and MLH1 ]]>
<![CDATA[Novrial, Dody]]>;
<![CDATA[Riwanto, Ignatius]]>;
<![CDATA[Sumitro, Sutiman Bambang]]>;
<![CDATA[Wijaya, Indra]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.8439
<![CDATA[Colorectal carcinogenesis induced by cigarette smoke requires at least 30-40 years. This long time duration causes an animal research conducted becomes relevant. This research was carried out to observe colorectal carcinogenesis due to cigarette smoke exposure in Wistar Rat. The observations focused on changes in epithelial morphology and expression of APC, MSH2 and MLH1. Twenty male Wistar rats inbreed strain were randomly allocated into control group and experimental group exposure to cigarettes smoke for 14 weeks and 28 weeks sequentially. Colorectal epithelial morphology was assessed on the histopathology examination, whereas the expression of APC, MSH2 and MLH1 was assessed on aspect of immunohistochemistry. The comparative analysis between the two groups was performed using non-parametric Mann-Whitney U test. Histology of colorectal epithelium showed pattern of colitis associated cancer that was significant both in 14 weeks and 28 weeks of treatment. This research indicated negative expression of APC, MSH2 and MLH1 in the colorectal cancer that were significant at 28 weeks of exposure. This research implies that chronic exposure to cigarette smoke can induce colitis associated colorectal cancer through decreased expression of APC, MSH2 and MLH1.]]>
<![CDATA[Antimalarial Herbal Plants in Kupang, Indonesia ]]>
<![CDATA[Ihwan, Ihwan]]>;
<![CDATA[Koda, Sari Hauliah Ahmad]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.5811
<![CDATA[Malaria is an infectious disease caused by Plasmodium parasite transmitted through female Anopheles mosquito bite. This disease is harmful because Plasmodium is known to be resistant to antimalarial drugs. The emergence of malaria resistance has raised concerns among researchers to look for any alternative antimalarial drugs. There are some plants potentially used as antimalarial drugs that should bewell-documented through scientific research. This study aimed to get to know plant species that has antimalarial activity in Kupang region. Method: This research applied qualitative descriptive method while using exploration method specifically in the sampling process. Every sample is then taken for preservation as herbarium and identified based on its morphological features using determination key handbook. The result showed that there are 17 species of antimalarial plant in 10 observational sites. Those species of antimalarial plants i.e. Tithonia diversifolia, Momordica charantia, Cyperus rotundus, Strychnos lingustrina, Andrographis paniculata, Callicarpa longifolia, Tinospora crispa, Piper betle, Plectranthus scutellarioides, Alstonia scholaris, Carica papaya, Amaranthus spinosus, Artocarpus champeden, Cassia siamea, Azadirachta indica, Helianthus annuus and Blumea balsamifera. Therefore, the availability of the plant species with antimalarial activity in Kupang needs to be explored and developed as an alternative approach for the society in treating Malarial diseases.]]>
<![CDATA[Chitosan as Chelating and Protective Agents from Lead Intoxication in Rat ]]>
<![CDATA[Marianti, Aditya]]>;
<![CDATA[Anatiasara, Debi]]>;
<![CDATA[Ashar, Fachrudyn Faisal]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.8943
<![CDATA[Increasing accumulation of lead in body causes serious health problems. Chelation is widely used to decrease lead level in body. This research aims the effectivity of chitosan in chelating lead and their protective ability toward liver and kidney for lead acetate-induced rat. Thirty rats were divided into 6 treatment groups. All groups, except control group, were administered by 175 mgkg-1 BW lead acetate. Positive control group was treated using EDTA 50 mgkg-1 BW. Treatment group 1,2, and 3 were treated using chitosan in dose of 64, 32, and 16 mgkg-1 BW dissolved in 2% acetic acid, respectively in 30 days. The effectivity of chitosan was compared to blood lead level. ALT and AST level were measured to determine the protective ability of chitosan. Normal function of kidney was assessed using creatinine level. Results showed that blood lead level from all treatment groups, except negative control group, had no significant difference from control group. EDTA and chitosan ability in chelating lead were proven by low level of AST, ALT, and creatinine in treatment groups. This indicated that there was no significant difference from control group. Chitosan capable of chelating lead and protecting kidney and liver from heavy metal.]]>
<![CDATA[Bioinformatics Education in Greece: A Survey ]]>
<![CDATA[Paraskevopoulou-Kollia, Efrosyni-Alkisti]]>;
<![CDATA[Bagos, Pantelis G]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.7257
<![CDATA[Bioinformatics is an interdisciplinary field, placed at the interface of Biology, Mathematics and Computer Science. In this work, we tried for the first time to investigate the current situation of Bioinformatics education in Greece. We searched the online resources of all relevant University Departments for Bioinformatics or relevant courses. We found that all the Departments of Biological Sciences include in their curricula courses dedicated to Bioinformatics, but this is not the case for Departments of Computer Science, Computer Engineering, or Medical Schools. Despite the fact that large Universities played a crucial role in establishing Bioinformatics research and education in Greece, we observe that Universities of the periphery invest in the field, by including more relevant courses in the curricula and appointing faculty members trained in the field. In order for us to triangulate we didnt confine ourselves to online resources and descriptive statistics but we also included interviews so as to have a more spherical view of the subject under discussion. The interviews provided useful insights regarding the teaching methods used by bioinformatics tutors, their attitudes and the difficulties they encounter. The tutors mentioned also the material that they choose, the audiences attraction techniques and the feedback they receive.]]>
<![CDATA[Production of Manooligomannan from Palm Kernel Cake by Mannanase Produced from Streptomyces Cyaenus ]]>
<![CDATA[Purnawan, Awan]]>;
<![CDATA[Yopi, Yopi]]>;
<![CDATA[Irawadi, Tun Tedja]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.9201
<![CDATA[The increase of public attention to health has prompted researchers to look for new sources of functional food. Palm Cake Kernel (PKC) waste was abundant in Indonesia, Oligosaccharide has an important benefit for human health. Recently oligosaccharide is not only important as an artificial sweetener, but also as a functional food component. This study was aimed to produce oligo-mannan enzymatically from PKC waste using mannanase derived from of Streptomyces cyaenus isolates of indigenous Indonesia. The enzyme concentration was determined by enzyme activity assay while oligo-mannan content in the PKC was analyzed using TLC and HPLC. Mannanase enzyme activity of 1706 U/ml on the second day of agitation 200 rpm at a temperature of 30C Hydrolysis of mannooligomannan by using mannanase produced by streptomyces cyaenus. The optimum mannanase enzyme activity obtained on day 2 with the value of the activity as much of 0.702 U/mL. The protein content of the 2nd day at an agitation speed of 150 rpm, 200 rpm, and 250 rpm, respectively, were 1783, 1950 and 2283 ppm. Streptomyces cyaenus is Indonesian original isolates potentially producing mannanase that can produce mannooligomannan.]]>
<![CDATA[The Diversity of Arthropods Predator on Wild Plant of Rice Field with and without Pesticides ]]>
<![CDATA[Meidalima, Dewi]]>;
<![CDATA[Kawaty, Ruarita Ramadhalina]]>;
<![CDATA[Gunawan, Erlan B.]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.5124
<![CDATA[Contaminated- agro ecosystem influences abundance and diversity of arthropods. The aims of this study were to analyze the diversity and abundance of pre-planting arthropod predators on wild plants in rice field with and without application of pesticides. The survey and direct observation of wild plants at the rice filed were conducted from January to March 2015, at 1 ha in Pemulutan and 1 ha in Musi 2 Palembang. Sampling of arthropod predator was conducted 8 times before the rice was grown, using insect nets. On the land without pesticide application was found 14 arthropod families which consists of 28 species and 15 families of wild plant consists of 25 species. On the land with pesticide application was found 8 arthropod families consists of 16 species and 15 wild plant families with 23 species. On the land without pesticide application was found High index of diversity (H=3.121) and low dominance (D=0.095), while on the land with pesticide application low index of diversity (H=2.602) and high dominance (D=0.171). It is the indicators of arthropods predator more varieties at the land without pesticide application compare to the land with pesticide application. This finding is very important for biological pest management in South Sumatra.]]>
<![CDATA[Antimicrobial and Antioxidant Activities of Resins and Essential Oil From Pine (Pinus merkusii, Pinuso ocarpa, Pinus insularis) and Agathis (Agathis loranthifolia) ]]>
<![CDATA[Tillah, Mardho]]>;
<![CDATA[Batubara, Irmanida]]>;
<![CDATA[Sari, Rita Kartika]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.8371
<![CDATA[The most common human pathogen that colonizes in a third of healthy people around the world are Staphylococcus aureus, and one of the materials allegedly able to overcome the pathogen is resin. Resin has been used in folk medicine for thousands of years to treat diseases. The antimicrobial activity of natural resins can be associated with a variety of organic compounds contained in them such as diterpenoids and triterpenoids. This research aimed to explore the antibacterial and antioxidant activities of Pinus merkusii, P. oocarpa, P. insularis, Agathis loranthifolia resins and essential oil. Resin was separated by distillation process to get essential oil and the residue was extracted using n-hexane, ethyl acetate (EtOAc), and methanol (MeOH). Antioxidant activity was performed by DPPH (1,1diphenyl-2-picryl hydrazyl) radical scavenging method. The antibacterial activity of resins and essential oil of the samples determined using the disc diffusion method against Staphylococcus aureus and Escherichia coli. The results showed that the yield of resin extract was ranging from 8.44 % to 95.56%. All extracts and essential oil could not inhibit E coli growth but inhibit the S. aureus growth. This experiment concluded that resin n-hexane extract from P.oocarpa was the most potent as antibacterial activity against S. aureus. All of the samples used had less potential antioxidant activity compared to positive control ascorbic acid. Result of this study show that pine resin from Indonesia has potential as an antibacterial agent.]]>
<![CDATA[Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510 ]]>
<![CDATA[Wahyuni, Dwi Kusuma]]>;
<![CDATA[Hafida, Siti Nur]]>;
<![CDATA[Ermayanti, Tri Muji]]>;
<![CDATA[Wardoyo, Bambang Prajoga Eko]]>;
<![CDATA[Purnobasuki, Hery]]>;
<![CDATA[Utami, Edy Setiti Wida]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i1.8223
<![CDATA[Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction.]]>
