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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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The Effect of Red Fruit Oil (Pandanus conoideus) to the Histophysiology of Rat (Rattus norvegicus) Liver Exposed to Cigarette Smoke Kio, Albertina Dhiu; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf Wachidah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12822

Abstract

Free radicals in cigarette smoke may interfere the balanced of body molecules and cause the damage of hepatic cells. This issues may be prevented by using red fruit oil (Pandanus conoideus L) due to its antioxidant content. This study aimed to evaluate the structure and physiological conditions of liver when the red fruit oil given to the rat that exposed by cigarette smoke. This study carried out for 35 days with female white rats as the sample. The samples were 20 female white rats divided into four groups: P0 (negative control), P1 (positive control), P2 (exposed by cigarette smoke with administration of red fruit oil 0,1 ml/BB), P3 (exposed by cigarette smoke with administration of red fruit oil 0,2 ml/weight). The data of ALT, AST, cell diameter, and hepatic weight were analyzed by using analysis of variants (ANOVA). The results showed that administration of red fruit oil containing antioxidants can reduce levels of ALT, AST, hepatocyte diameter, and increase liver weight, although the difference is not significant compared to the control group and does not affect the eating and drinking behavior so as not to affect the bodys metabolism. The conclusion of red fruit oil can reduce ALT, AST, hepatocyte diameter and increase hepatic weight due to exposure to cigarette smoke, although the difference is not significant to the control group, and does not affect the eating and drinking behavior, so good for consumption because it gives a beneficial effect on health liver organ.
Ketahanan Hidup Sel Acetobacter xylinum pada Pengawetan secara Kering-Beku Menggunakan Medium Pembawa (Viability of A. xylinum Subjected to Freeze Drying Using Carrier Media) Dewi, Pramesti
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.546 KB) | DOI: 10.15294/biosaintifika.v1i1.44

Abstract

A research on the use of sucrose and lactose as carrier media to protect Acetobacter xylinum cell subjected to freeze drying has been done. The aim of the research was to know the number of the viable cells from dried culture and to know the concentration of the carrier medium that would give best result. The best result is sucrose at the concentration of 15% that produced 28.2 x 106 viable cells/ ml of rehidrated culture. The rehidrated culture used in the research was Schramm & Herstin medium.
Improving Students Scientific Reasoning and Problem-Solving Skills by The 5E Learning Model Susilowati, Sri Mulyani Endang; Anam, Khaerul
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.12022

Abstract

Biology learning in MA (Madrasah Aliyah) Khas Kempek was still dominated by teacher with low students involvement. This study would analyze the effectiveness of the 5E (Engagement, Exploration, Explanation, Elaboration, Evaluation) learning model in improving scientific knowledge and problems solving. It also explained the relationship between students scientific reasoning with their problem-solving abilities. This was a pre-experimental research with one group pre-test post-test. Sixty students of MA Khas Kempek from XI MIA 3 and XI MIA 4 involved in this study. The learning outcome of the students was collected by the test of reasoning and problem-solving. The results showed that the rises of students scientific reasoning ability were 69.77% for XI MIA 3 and 66.27% for XI MIA 4, in the medium category. The problem-solving skills were 63.40% for XI MIA 3, 61.67% for XI MIA 4, and classified in the moderate category. The simple regression test found a linear correlation between students scientific reasoning and problem-solving ability. This study affirms that reasoning ability is needed in problem-solving. It is found that application of 5E learning model was effective to improve scientific reasoning and problem-solving ability of students.
Correlation between Food Intake and Health Status with the Nutritional Status of School Children Age 9-11 in Semarang City Ali, Aiman Farag Mohammed; Muis, Siti Fatimah; Suhartono, Suhartono
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6488

Abstract

Malnutrition, a major risk factor for a number of infectious diseases, including acute upper respiratory tract infections (AURTI), is common in developing countries. Nutritional status is an important index of the quality of life. Objectives:To analyze the correlation between food intake and health status to nutritional status of 9-11 years old children in Semarang. The study was a correlation study carried among school children in Semarang aged 9-11 years old. Data are presented in the descriptive analyses and Spearman correlation. Overall, food intake (energy and protein) of 9-11 years old children in Semarang is normal with ? 90% RDA, health status of them was satisfactory (very low AURTI incidence),and their nutritional status were mostly normal. There was a correlation between energy intake with nutritional status with indicators BMI, and z-score of W/A and H/A, but there was no correlation between protein intake and AURTI with nutritional status. Energy and food intake of the children correlate with all nutritional status being studied. It should be suggested to parents to implement balanced diet, to avoid the development of obesity among elementary school children through nutrition education to prevent malnutrition as well as obesity.How to CiteAli, A. F. M., Muis, S. F., & Suhartono, S. (2016). Correlation between Food Intake and Health Status with The Nutritional Status of School Children Age 9-11 in Semarang City. Biosaintifika: Journal of Biology & Biology Education, 8(3), 249-256.
Effectivity of Pedada Fruit (Sonneratia caseolaris) Extract to The Level of Sgot and Sgpt in Rat Treated by Paracetamol Induction Lestari, Ratih Devi; Susanti, R
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3541

Abstract

The study was aimed to determine the effectiveness of pedada fruit extract as a hepatoprotector in the experimental rat that fed by toxic dose of paracetamol. The total of 30 white rats (Wistar strain, two months age, and 150-200 g weight) were randomly divided into 5 groups. Group I (normal control) only given distilled water for 7 days). Group II (negative control) that given distilled water for 7 days and then treated by 270 mg/head single dose of paracetamol. Group III, IV, and V (treatment group) were given a pedada fruit extract at a dose of 28 mg/head/day, 56 mg/head/day, and 84 mg/head/day for 7 days and then treated by 270 mg/head single dose of paracetamol. On the 9th day of treatment, the blood samples were taken and were further measured for its SGOT and SGPT level using photometry enzymatic method. The result of LSD test on SGOT and SGPT data showed that III, IV, and V groups were not significantly different to the group I (p>0.05). However, it significantly different with the group II (p <0.05). Data of SGOT showed that group IV were significantly different (p <0.05) with the group V. Whereas, the data of SGPT among groups III, IV, and V were not significantly different (p>0.05). The result of linier regression test indicated that dose 28 mg/head was the most effective dose. It was concluded that pedada fruit extract was able to provide a hepatoprotective effects in rats that fed by toxic dose of paracetamol and most effective dose as a hepatoprotector was 28mg/head/day.Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak buah pedada sebagai hepatorotektor tikus putih yang diberi parasetamol dosis toksik.Sebanyak 30 ekor tikus putih (strain Wistar jantan berumur dua bulan dengan berat badan 150-200 g) dibagi secara acak dalam lima kelompok.Kelompok I (kontrol normal) diberi aquadest selama tujuh hari.Kelompok II (kontrol negatif),diberi aquadest selama tujuh hari dilanjutkan pemberian parasetamol 270 mg/ekor dosis tunggal.Kelompok III, IV, dan V (kelompok perlakuan) diberi ekstrak buah pedada pada dosis 28 mg/ekor/hari, 56 mg/ekor/hari, dan 84 mg/ekor/hari selama tujuh hari dilanjutkan pemberian parasetamol 270 mg/ekor dosis tunggal. Hari ke-9 darah diambil dan diukur kadar SGOT dan SGPT dengan metode fotometri enzimatik. Hasil uji LSD data SGOT dan SGPT menunjukkan bahwa kelompok III, IV, dan V tidak berbeda nyata terhadap kelompok I (p>0,05), namun berbeda nyata terhadap kelompok II (p<0,05). Data SGOT kelompok IV berbeda nyata (p<0,05) dengan kelompok V. Data SGPT tidak ada perbedaan nyata (p>0,05) antara kelompok III, IV, dan V. Hasil uji regresi linier, dosis 28 mg/ekor adalah dosis paling efektif.Disimpulkan bahwa ekstrak buah pedada mampu memberikan efek hepatoprotektor pada tikus yang diberi parasetamol dosis toksik dan dosis yang paling efektif sebagai hepatoprotektor adalah dosis 28 mg/ekor/hari.
The Expression of mRNA LMP1 Epstein-Barr Virus from FFPE Tumour Biopsy: a Potential Biomarker of Nasopharyngeal Carcinoma Diagnosis Wahyono, Daniel Joko; Gumilas, Nur Signa Aini; Sulistyo, Hidayat
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9028

Abstract

Nasopharyngeal carcinoma (NPC) is a multifactorial disease that is endemic geographically in the world. Indonesian population has a highly incidence rate that is 6.2/100,000 people year. The pathogenesis of NPC is more directly reflected by carcinoma-specific viral transcriptional activity at the site of primary tumour. Epstein-Barr virus (EBV) infection in NPC is reflected by the expression of EBV latent and lytic gene. In fact, mRNA Latent Membrane Protein 1 (LMP1) EBV expression was an important latent infection biomarker. The aim of this study was to determine a potential use of relative expression of mRNA LMP1 EBV from formalin-fixed paraffin embedded (FFPE) tumour biopsy in NPC as a tumour biomarker. This reseach design was a cross sectional study. The samples were the archived specimens of FFPE tumour biopsy from NPC WHO-3 patient which were collected from untreated patients from 2014 in the Department of Pathology Anatomy, Prof. dr. Margono Soekarjo Hospital, Purwokerto. The expression of mRNA LMP1 EBV expression was determined by RT-PCR technique. The positivity of mRNA LMP1 EBV expression was 51.9%, indicating a moderate positivity. The result proved that the expression of mRNA LMP1 EBV from FFPE NPC WHO-3 tumour biopsy was a potential biomarker of NPC diagnosis. The molecular methods would improved the management of NPC, particularly in the histopathological diagnosis of NPC.
Development of Natural Sciences Module with Reflective Learning Journal to Enhance Student’s Reporting-Interpretative Skills Zulfah, Hadaina; Aznam, Nurfina
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14319

Abstract

One of the successful factors of the curriculum 2013 implementation is the availability of appropriate and relevant teaching materials. Teaching materials can be textbooks, interactive videos , virtual labs, maket and so on. Unfortunately, there are still found many materials that are less in accordance with the curriculum of 2013. Therefore it should be developed a more appropriate teaching materials, one of them is a natural sciences module with reflective learning journal. The development of natural sciences modulewas done with research that aims to develop and analyze the effectiveness of natural sciences modulewith reflective learning journals. This research was an R&D research using development research procedure according to Thiagarajanthat known as 4D (define, design, develop and dessiminate). The data collection was done by test and non-test technique. Instrument that used in non test technique are interview guides, product validation sheet, students’ response sheet to the readability of science nature modul with reflective learning journal., meanwhile the instrumen that used in test technique is essay question with reporting-interpretative skills’s indicators. The results showed that the leligibility of the natural sciences module with reflective learning journal was categorized as approriate and effective to improve student’s reporting-interpretative skills.
Potensi Sumberdaya Alam Hayati Kawasan Mangrove Pasar Banggi Kabupaten Rembang Sebagai Objek Ekowisata -, Kusaeri; Putro, Sapto P.; Wasiq, Jafron
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3955

Abstract

Kawasan Mangrove Pasar Banggi merupakan salah satu tegakan mangrove yang masih baik dan sudah direboisasi sejak tahun 1960-an. Kawasan tersebut memiliki sentra persemaian mangrove yang cukup dikenal oleh masyarakat luas di Pulau Jawa. Penelitian ini bertujuan untuk mengetahui kondisi vegetasi dan kualitas lingkungan mangrove serta menyusun rekomendasi strategi pengembangan ekowisata mangrove di kawasan tersebut. Metode penelitian menggunakan deskriptif analitik melalui pendekatan kuantitatif. Hasil penelitian menunjukkan bahwa hutan mangrove Pasar Banggi seluas 14,88 ha dalam kondisi cukup baik dengan identifikasi jenis mangrove yang ditemukan Avicennia marina, Rhizophora apiculata, Rhizophora mucranata, Rhizophora stylosa, Sonneratia alba, dan Sonneratia caseolaris. Indeks Nilai Penting (INP) untuk vegetasi mangrove tertinggi terdapat pada jenis Rhizophora mucronata sebesar 281,82%, dan Indeks Kesesuaian Wisata Mangrove pada 9 lokasi (plot) diperoleh data 61,54% yang masuk kedalam kategori sesuai bersyarat. Strategi pengembangan potensi mangrove berdasarkan Matriks Grand Strategy SWOT berada pada kuadran I yang berarti menggunakan strategi agresif. Dalam strategi ini yang diprioritaskan adalah penentuan master plan zonasi pemanfaatan secara terpadu, manajemen pengelolaan objek ekowisata yang profesional serta membangun komitmen semua pihak dalam menjaga kelestarian lingkungan.Mangrove areas of Pasar Banggi is one of the mangrove stands has been reforested since the 1960s. The region has a mangrove nursery centers well known by the public on Java Island. This study aimed to determine the condition of mangrove vegetation and environmental quality. It also aimed to make a recommendation for mangrove eco-tourism development strategy. The research method used was descriptive qualitative analytical approach. The results showed that mangrove forests of Pasar Banggi was 14.88 ha in good condition with the identification of mangrove species found were Avicennia marina, Rhizophora apiculata, Rhizophora mucranata, Rhizophora stylosa, Sonneratia alba, and Sonneratia caseolaris. The importance value index for the highest mangrove vegetation found in Rhizophora mucronata by 281.82%, and travel suitability index of mangrove at 9 locations (plots) acquired 61.54% of data that goes into the category of conditionally appropriate. Mangrove potency development strategy was based on SWOT Matrix Grand Strategy was in quadrant I, which means using aggressive strategy. The strategy priority was the determination of the zoning master plan for integrated data collection, the professional management of ecotourism object and to raise the commitment of all parties to keeping environment sustainability.
Peranan Tanaman terhadap Pencemaran Udara di Jalan Protokol Kota Semarang Tri Martuti, Nana Kariada
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i1.2572

Abstract

Peningkatan jumlah kendaraan bermotor berpotensi meningkatkan pencemaran udara terutama di jalan-jalan protokol. Untuk mengurangi semakin tingginya bahan pencemar yang dihasilkan kendaraan bermotor, perlu adanya pohon-pohon yang berfungsi sebagai penyerap dan penjerap bahan pencemar dan debu di udara yang dihasilkan kendaraan bermotor. Tujuan penelitain ini adalah mengetahui peranan tanaman di jalan-jalan protokol Kota Semarang dilihat dari kualitas dan kuantitasnya. Data mengenai jenis tanaman peneduh yang ada di jalan protokol Kota Semarang dihitung dengan metode line intercept. Hasil penelitian menunjukkan bahwa kualitas udara di Kota Semarang sudah cukup mengkhawatirkan, hal ini dipandang dari kadar CO yang relatif tinggi. Hasil penelitian terhadap jenis tanaman yang ada di jalan protokol Kota Semarang, dapat diketahui jenis tanaman yang ditanam di 5 jalan protokol Kota Semarang berjumlah 29 jenis. Jenis maupun jumlah tanaman yang ada pada masing-masing stasiun penelitian tidak berpengaruh positif terhadap kadar bahan pencemar udara yang ada. Simpulan dari penelitian ini ialah kualitas maupun kuantitas tanaman yang ada pada jalan-jalan protokol lokasi penelitian tidak berpengaruh positif terhadap kadar bahan pencemar udara yang ada. Hal ini dikarenakan jenis dan jumlah tanaman pada masing-masing jalan protokol tidak sesuai dengan tanaman peneduh yang mempunyai fungsi sebagai penjerap dan penyerap polutan udara.The increasing number of motor vehicles might potentially increase the air pollution in main roads. To reduce the increasing concentration of pollutants generated by motor vehicles, the trees are planted to absorb the pollutants and the dust in the air. The objective of the research was to understand the role of the plants along the main roads in Semarang City, from point of view of quality and quantity. Data on the kind and density of shedding plants along the main roads of Semarang City was collected using line intercept method. Result showed that the air quality of Semarang City has been worrying in terms of the high concentration of CO. There were 29 kinds of trees planted along 5 main roads in Semarang City. The kind and the number of plants in each station did not influence positively to the concentration of the air pollutants. It was concluded that the quality and the quantity of the plants along the study sites did not positively influence the concentration of the air pollutants. It was presumed that the kind and the number of plants along each ain road in Semarang was not appropriately functioning as the absorbants of the air pollutants.
Antimalarial Herbal Plants in Kupang, Indonesia Ihwan, Ihwan; Koda, Sari Hauliah Ahmad
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.5811

Abstract

Malaria is an infectious disease caused by Plasmodium parasite transmitted through female Anopheles mosquito bite. This disease is harmful because Plasmodium is known to be resistant to antimalarial drugs. The emergence of malaria resistance has raised concerns among researchers to look for any alternative antimalarial drugs. There are some plants potentially used as antimalarial drugs that should bewell-documented through scientific research. This study aimed to get to know plant species that has antimalarial activity in Kupang region. Method: This research applied qualitative descriptive method while using exploration method specifically in the sampling process. Every sample is then taken for preservation as herbarium and identified based on its morphological features using determination key handbook. The result showed that there are 17 species of antimalarial plant in 10 observational sites. Those species of antimalarial plants i.e. Tithonia diversifolia, Momordica charantia, Cyperus rotundus, Strychnos lingustrina, Andrographis paniculata, Callicarpa longifolia, Tinospora crispa, Piper betle, Plectranthus scutellarioides, Alstonia scholaris, Carica papaya, Amaranthus spinosus, Artocarpus champeden, Cassia siamea, Azadirachta indica, Helianthus annuus and Blumea balsamifera. Therefore, the availability of the plant species with antimalarial activity in Kupang needs to be explored and developed as an alternative approach for the society in treating Malarial diseases.

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