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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 18   19   20   21   22 
Technological Pedagogical and Content Knowledge of Biology Prospective Teachers Suryawati, Evi; Linggasari, Mariani Natalina; Arnentis, Arnentis
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11270

Abstract

This research was conducted for analyzing Technological Pedagogical and Content Knowledge (TPACK) of biology prospective teachers in Riau Province. The study was carried out to fulfill Indonesian Governments policy on Indonesian Qualification Framework (IQF) 6-Level. This development research aimed to analyze, design and develop a model to enhance the competency and competitiveness of Education College graduates to meet their professional, social and industrial needs. This paper discussed a preliminary study of the developed models for strenghtening the prospective teachers from Biology Education Departement, conducted through a survey of 243 students from four different colleges of education in Riau Province. The data were collected through a test and interview. The instruments used contained seven variables of knowledge, namely Technology, Paedagogy, Content, Technological Paedagogy, Technological Content, Technological knowledge and TPACK. The instruments was improved through their validity and reliability test. The TPACK performance of Prospective Biology teachers seemed low. Based on the need analysis two products will be generated. First, a lesson design and the second was a work books. This study had an implications for increasing the competence of biology education graduates. The lesson design and competency test instruments could be used for College of Biology Education and teacher professional education in developing competency test for selection system. The TPACK workbook could be used as teacher enrichment in sustainable profession development such as classroom action research, learning innovation contest, and science teacher olympiad.
The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro Utami, Edy Setiti Wida; Hariyanto, Sucipto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5165

Abstract

Dendrobium antennatum has high economic value as cut flowers and flowerpots. Like orchid seeds in general, D. antennatum is difficult to germinate under natural conditions. This study aimed to determine the effect of coconut water on seed germination and embryo development, as well as the effect of NAA on shoots development of D. antennatum. This study consisted of two stages. In the first stage, the 12 weeks-old seeds after pollination were sown on MS medium containing 2 g/L peptone + 0%; 5%; 10%; and 20% coconut water. After 8 weeks of culture, the seeds germinated and the shoot formed were recorded. The highest in seed germination (92.2%) and the formation of shoots (51.4%) were obtained when seeds were cultured on MS medium containing 2 g/L peptone + 20% coconut water. In the second stage, the shoots were sub-cultured on MS medium containing 1 mg/L thidiazuron + 0 mg/L; 1 mg/L; 2 mg/L; and 3 mg/L NAA. After 16 weeks of sub-culture, the height of plantlets, the length of the roots and leaves, number of leaves and roots formed were recorded. MS medium containing 1 mg/L thidiazuron + 1 mg/L NAA was the most suitable for the shoots development of D.antennatum. The embryo development of D.antennatum in vitro begins with the enlargement of embryo, with further it emerges from the seed coat (germinated) followed by the formation of the apical meristems to form the shoots and the roots.How to CiteUtami, E. S. W., & Hariyanto, S. (2016). The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro. Biosaintifika: Journal of Biology & Biology Education, 8(2), 165-171.
Analisis Kualitas Fisiko Kimia dan Kadar Logam Berat pada Ikan Mas (Cyprinus carpio L.) dan Ikan Nila (Oreochromis niloticus L.) di Perairan Kaligarang Semarang Kusuma Dewi, Nur; Prabowo, Rossi; Kariada Trimartuti, Nana
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3106

Abstract

Tujuan penelitian untuk mengetahui kualitas fisiko kimia air sungai Kaligarang dan kandungan logam berat pada ikan mas dan ikan nila yang hidup di sungai tersebut. Penelitian ini merupakan penelitian eksperimen lapangan, menggunakan Karamba Jaring Apung. Parameter fisiko-kimia yang diukur adalah suhu, pH, DO, alkalinitas, kesadahan, kecerahan dan kecepatan arus serta kandungan logam berat di air sungai, menggunakan metode Atomic Absorption Spectrophotometry (AAS). Kadar logam berat Cd, Pb dan Hg juga dianalisis pada ikan mas dan ikan nila Hasil penelitian menunjukkan bahwa suhu, pH, DO, alkalinitas, kesadahan, kecerahan dan kecepatan arus masih berada dalam kisaran yang layak bagi kehidupan ikan. Kadar Cd, Pb dan Hg di perairan masing-masing adalah sebesar 0,006 mg/L, 0,01 mg/L dan 0,0006 mg/L. Kadar Cd, Pb dan Hg pada hati ikan nila berturut-turut 0,000407 mg/kg, 0,000812 mg/kg dan 0,000575 mg/kg, sedangkan pada ikan mas 0,000307 mg/kg, 0,000269 mg/kg dan 0,000349 mg/kg. Kesimpulan; air sungai Kaligarang terkontaminasi logam berat Cd, Pb dan Hg dan ikan yang hidup di sungai tersebut telah mengakumulasikan ketiga logam berat tadi.The aim of this study is to determine the quality of water in Kaligarang based on the physicochemical characteristics and heavy metal content in carp and tilapia fish live in the river. This study is a field experiment, by using Karamba floating net; the fish were caught and maintained. The physicochemical parameters of the water measured were temperature, pH, oxygen, alkalinity, hardness and brightness, speed of the flow and content of heavy metals. The heavy metal content was measured using Atomic Absorption Spectrophotometry (AAS). Levels of heavy metals (Cd, Pb and Hg) were also analyzed in carp and tilapia. The results showed that temperature, pH, oxygen, alkalinity, hardness, brightness and speed of the flow were still within the appropriate range for the life of the fish. Levels of Cd, Pb and Hg in water were amounted to 0.006 mg/L, 0.01 mg/L and 0.0006 mg/L, respectively. Surprisingly, the levels of Cd, Pb and Hg in liver of tilapia were 0.000407 mg/kg, 0.000812 mg/kg and 0.000575 mg/kg, respectively, whereas in carp were 0.000307 mg/kg, 0.000269 mg/kg and 0.000349 mg/kg, respectively. It can be concluded that, Kaligarang River was contaminated with heavy metals Cd, Pb and Hg. This contamination leads to the accumulation of heavy metals in fish that live in the river.
Diversity and Distribution of Myrmecophytes in Bengkulu Province Safniyeti, Safniyeti; Sulistijorini, Sulistijorini; Chikmawati, Tatik
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9285

Abstract

Myrmecophyte is a common medicinal plant used by local people in Indonesia for treating various diseases especially in Papua. Bengkulu province is one of the Myrmecophyte habitats, but there has no report on its identity and distribution. The objectives of this research were to identify the diversity and analyze the Myrmecophytes distribution as well as factor affecting its presence. This study used purposive sampling method by exploring the area where Myrmecophytes commonly found. The Myrmecophyte distribution based on host tree was analyzed using Morishita index and the autecological analysis of abiotic factors was performed using Principal Component Analysis (PCA) generated from Minitab 16. The results of this research showed that there were two species of Myrmecophytes in Bengkulu province, namely Hydnophytum formicarum and Myrmecodia tuberosa, as well as two variants of M. tuberosa i.e. M. tuberosa armata and M. tuberosa siberutensis. The distribution of Myrmecophytes based on host tree was mostly randomly scattered in Central Bengkulu regency, Seluma, North Bengkulu, South Bengkulu, and Kaur. Their distributions were affected by light intensity and temperature. The data of this research can be used as basic information for carried out conservation efforts in Bengkulu province. The abundance of Myrmecophytes is also used as a source of additional income for local people in Bengkulu province.
Microbiological and Physicochemical Characteristics of Inasua Traditional Fish Fermented from Maluku Islands Mahulette, Ferymon; Mubarik, Nisa Rachmania; Suwanto, Antonius; Widanarni, Widanarni
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13537

Abstract

Based on the raw materials, inasua consists of two types namely inasua with sap and inasua without sap. Research of inasua with sap has never been done and considered as the novelty of this research. The sensory characteristics and shelf life of two types of inasua were different. The research aims to analyze the microbiological and physicochemical characteristics of two types of inasua during fermentation. The microbiological analyzes include the total number of bacteria and lactic acid bacteria, while physicochemical analyzes include temperature, pH, water activity, proximate analysis, salt, alcohol, histamine, amino acids and fatty acids contents. The total number of bacteria and lactic acid bacteria has decreased during fermentation. At the end of the fermentation the total number of bacteria and lactic acid bacteria inasua with sap were 3.2x107 CFU/g and 3.0x107 CFU/g, while inasua without sap were 5.4x105CFU/g and 3.5x105 CFU/g, respectively. The moisture, protein, alcohol contents and water activity decreased, otherwise the salt, fat, ash, amino acids, and fatty acids contents increased during fermentation. Generally, microbiological and physicochemical characteristics of inasua with sap was better than inasua without sap. The results of this research to improve the quality of this fermentation product in the future.
Deteksi Daging Babi Pada Produk Bakso di Pusat Kota Salatiga Menggunakan Teknik Polymerase Chain Reaction Fibriana, Fidia; Widianti, Tuti; Retnoningsih, Amin; -, Susanti
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.3928

Abstract

Teknik PCR mempunyai kemampuan yang sensitif untuk deteksi keberadaan daging babi di dalam daging segar maupun produk olahan yang dicampur dengan bahan lain. Tujuan penelitian ini untuk mengetahui apakah produk bakso yang dijajakan di pusat kota Salatiga mengandung daging babi. Teknik stratified random sampling digunakan untuk mengambil sampel bakso yang dijajakan 13 warung bakso dari 25 warung bakso yang tersebar di pusat Kota Salatiga. Isolasi dan purifikasi DNA sampel bakso, daging sapi, dan daging babi menggunakan metode isolasi DNA jaringan hewan. DNA hasil isolasi dilanjutkan proses PCR menggunakan primer p14 untuk mengamplifikasi lokus PRE-1 pada genom babi. Proses amplifikasi DNA dengan program denaturasi awal pada suhu 93 C selama 2 menit, diikuti 45 siklus terdiri atas denaturasi 93 C selama 1 menit, annealing 62 C selama 30 detik, ekstensi 72 C selama 1 menit, kemudian diakhiri ekstensi 72 C selama 2 menit. Produk PCR yang diharapkan muncul berukuran 481bp. Hasil elektroforesis gel agarose 1,2% pada produk PCR menunjukkan adanya pita DNA spesifik berukuran 481 bp pada daging babi dan sampel bakso nomor tiga belas, sehingga disimpulkan warung bakso nomor tiga belas produk baksonya mengandung daging babi.PCR technique has the ability to be sensitive to the detection of the presence of pork in fresh meat and processed products are mixed with other materials. The aim of this research to determine whether the product meatballs are sold in downtown Salatiga containing pork. Stratified random sampling technique is used to take samples of meatballs stall which sold 13 of the 25 meatballs stalls in the Salatiga City centre. Isolation and purification of DNA samples of meatballs, beef, and pork using DNA isolation method of animal tissue. DNA isolation results continue the process of PCR using primers to amplify p14 locus PRE-1 in the pig genome. DNA amplification process with initial denaturation program at a temperature of 93 C for 2 min, followed by 45 cycles consisting of denaturation 93 C for 1 min, annealing 62 C for 30 seconds, extension 72 C for 1 minute, then topped extension 72 C for 2 minutes. PCR products were expected to appear sized 481bp. Results of a 1.2% agarose gel electrophoresis of PCR products indicate a specific DNA band sized 481 bp on pork and meatball sample number thirteen, so it concluded meatball stall number thirteen baksonya products containing pork.
KETERTARIKAN LALAT BUAH BACTROCERA PADA EKSTRAK OLAHAN LIMBAH KAKAO BERPENGAWET Indriyanti, Dyah Rini; Subekti, Niken; -, Latifah
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2272

Abstract

Lalat buah Bactrocera spp. (Diptera: Tephritidae) merupakan salah satu hama pen ting menyerang tanaman buah-buahan dan sayuran. B. carambolae di laboratorium tertarik pada olahan limbah kakao. Hasil uji coba di lapangan belum memuaskan karena olahan limbah kakao mudah rusak. Tujuan penelitian ini mengkaji respon lalat buah Bactrocera yang diberi umpan ekstrak olahan limbah kakao berpenga wet. Pengawet yang digunakan yakni: Natrium klorida (NaCl), Natrium benzoat (C7H5NaO2) dan Potasium sorbat (C6H7KO2). Konsentrasi yang dipakai masing-masing pengawet 0,1%; 0,2% dan 0,3%. Pengamatan dilakukan selama satu ming gu. Hasil pengamatan menunjukkan bahwa daya tahan limbah yang diberi penga wet dan yang tidak dilihat secara secara fisik (warna dan tekstur) tidak berbeda nyata, namun ada perbedaan bau. Limbah yang tidak diberi pengawet ada kecen derungan baunya tidak sedap dibanding yang diberi pengawet. Hal ini yang mempengaruhi ketertarikan lalat terhadap olahan limbah kakao. Respon ketertarikan lalat Bactrocera terhadap olahan limbah kakao yang diberi pengawet berbeda antara satu dengan yang lain. Respon ketertarikan tertinggi Bactrocera cenderung pada olahan limbah kakao yang diberi pengawet Natrium klorida 0,3%, Potasium sorbat 0,2% dan Natrium benzoat 0,1%.The fruit fly Bactrocera spp. (Diptera: Tephritidae) is one of the important pests attacking crops of fruits and vegetables. In the laboratory, B. carambolae was attracted by the processed cocoa waste. The results of field trials have not been satisfactory yet, because the processed cocoa waste was easily damaged. The purpose of the study wast to examine the response of Bactrocera to the bait made of processed cocoa extract waste containing preservatives. The preservatives used were: Sodium chloride (NaCl), sodium benzoate (C7H5NaO2) and potassium sorbate (C6H7KO2). The concentration of each preservative was 0.1%; 0.2% and 0.3%. A one-week observation was made. The result showed that there was no significant difference on the durability of the preserved waste and the non-preserved waste. This can be seen from the physical properties (color and texture). However, there was difference in odor. The non-preserved waste tended to produce unpleasant smell than the preserved waste. This odor affected the capability to attract the flies to the processed cocoa waste. The Bactrocera was highly responsive to the waste containing preservative 0.3% sodium chloride, followed by 0.2% potassium sorbate and 0.1% sodium benzoate.
Potensi Trichoderma harzianum sebagai Biofungisida pada Tanaman Tomat ( Trichoderma harzianum Potency as a Biofungicide on Tomato Plant ) Herlina, Lina
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.35

Abstract

Fusarium disease of tomato plant is caused by Fusarium oxysporum. For gaining high quality and stable tomato production but physically construction, natural management of environment balancing, consistency, and stability is a must. A kind effort that should be tried is using natural agent, Trichoderma. This research aims to know the effect of Trichoderma implementation towards Fusarium oxysporum growth, and Tomato fruit production and vitamin content. There are two steps of this research. The first is seeing Trichoderma perform towards Fusarium oxysporum growth in a variation of incubation periode (7, 14. 21 and 28 days). The second, is giving Trichoderma agent on the tomato planting in some dozez (g), such as 0,10,20,30,40 and 50. The parameter are Fusarium oxysporum growth limits area, fruit weight per plant, and fruit vitamin C content. The research shows that Trichoderma giving are effecting Fusarium oxysporum growth, tomato fruit weight and fruit vitamin C content in a significance way. The highest level of vitamin C content (84,78 mg/matterial) and fruit weight (550 g) is gained on Trichoderma implementation which has incubation day of 21 (19.08 % ) and dozez of 40 grams.   Keywords : biofungisida,Trichoderma, Fusarium oxysporum , tomat
An Effort of Mutation Breeding by Oryzalin and Gamma Rays on Wild Raspberry (Rubus sp.) in Cibodas Botanical Garden Surya, Muhammad Imam; Ismaini, Lily; Destri, Destri; Normasiwi, Suluh
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6559

Abstract

Currently, Cibodas Botanical Garden (CBG) has a domestication programme of wild raspberries (Rubus sp.) from Indonesia mountain forest. One of the activities in the domestication programme is fruit breeding. In order to improve the quality of wild raspberry, two mutagens i.e. chemical (oryzalin) and physical (gamma rays) mutagens were used in the breeding programme. Moreover, seed of wild raspberries from the collection of CBG were used in the programme of mutation breeding. The results showed that each species of wild raspberries has different response on the mutagen. Percentage of seed germination were affected by mutagen of oryzalin and gamma rays. Rubus fraxinifolius and Rubus rosifolius seeds were still able to germinate after treated by 100 M of oryzalin. In the other hand, with increase in radiation above 300 Gy a reduction in percentage of germination was observed in M1 generation of Rubus lineatus and Rubus chrysophyllus.How to CiteSurya, M. I., Ismaini, L., Destri, D. & Normasiwi, S. (2016). An Effort of Mutation Breeding by Oryzalin and Gamma Rays on Wild Raspberry (Rubus spp.) in Cibodas Botanical Garden. Biosaintifika: Journal of Biology & Biology Education, 8(3), 331-335.
Traditional Knowledge on The Animal Utilization by The Hatam Tribe of Manokwari, West Papua Province Sutarno, Simon; Qayim, Ibnul; Muhadiono, Ignatius; Purwanto, Yohanes; Zuhud, Ervizal A. M.
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.9441

Abstract

Animal utilization in traditional community life is an integral part of traditional knowledge itself. This research aims to reveal the Hatam peoples traditional knowledge about the utilization of animals in their lives. The method used is the exploratory survey method. The respondents were determined purposively using the snowball sampling technique. The results showed that there are at least 55 species of animals commonly used by the Hatam people. Based on the forms of utilization, the animals can be divided into six groups: consumption, traditional medicine, specific meanings, traditional technology and arts, significant value, and traditional purposes. Wild animals are most widely used for the living needs of the Hatam people. The traditional hunt that is limited and the communal land ownership system (ulayat system) practiced by the Hatam people have indirectly helped preserve and ensure the availability of wildlife for the Hatam peoples needs. In addition to being a form of documentation of Hatam peoples traditional knowledge, the results of this research also have significance for the development of science especially in the field of ethnozoology. The form of interaction between Hatam people and animals revealed in this research can also serve as a reference for designing development policies related to the presence of Hatam people in the Arfak mountains.

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