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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Potential of Sodium Alginate in Sargassum sp. in Lotion Preparation to Treat Incision Wound in Mice Angelina, Amelia Cahya; Yudiati, Ervia; Pringgenies, Delianis
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.22539

Abstract

Sargassum sp. is a known genus of brown seaweed whose dominant component is alginic acid or alginate. Various studies found that alginate having proinflammatory activity, so it may have potential to improve the wound healing process. This study aimed to obtain sodium alginate extract and to know the potency of the sodium alginate extract in incision wound treatment in mice abdomen. Lotion application was performed once a day in a span of five consecutive days. Observation on the wound was carried out to obtain data on blood coagulation, tissue reparation, and fibroblast development rate on the wound using histology. The results showed that the average yield from the Sargassum sp. extraction process was 31.3%. Quality control of the prepared lotion included organoleptic test; light brown coloration, lotion specific odor, semi- solid texture. The acidity of the lotion was measured as 6.61 with 6.731 cp of viscosity. The lotion adhesiveness test showed 0.21 seconds and the lotion was shown to be oil-in-water. Application of the lotion on incision wounds made in the abdomen of rats showed blood coagulation on the first day and onset of wound reparation process on the second day. Wound observation by the third day showed that the inflicted area had undergone a near-complete reparation. The study also showed that on the fifth day of reparation Sargassum sp. extract lotion treatment group showed fibroblast formation, as opposed to the non-extract lotion treatment group. Previous study use alginates as wound dressing material, but in this study alginate used as active ingredient in wound healing treatment. Therefore, this study concludes that sodium alginate in Sargassum sp. seaweed has a potential application in the field of medicine.
Total Phenol Content of Avicennia marina Leaf and Its Relationship to the Environmental Quality Hastuti, Endah Dwi; Izzati, Munifatul; Darmanti, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23957

Abstract

Environmental dynamic in the coastal area is suggested to affect the metabolite concentration in mangrove plants. This research aimed to study the concentration of total phenol in A. marina leaf and to analyze the effect of environmental parameters on total phenol content dynamics. Environmental parameters studied in this research was temperature, pH, DO, and salinity, as well as N, P, and C sediment content. Data analysis was carried out through multiple regression of natural logarithm transformed data. Laboratory analysis resulted the value of total phenol content in A. marina leaf ranging from 0.88 – 1.62% with the average concentration of 1.28 ± 0.28%. Regression analysis resulted the significant effect of temperature, DO, and sediment content P and C expressed in the formula: ln(TP) = 31.229 – 7.224ln(T) – 0.067ln(DO) – 1.054ln(P) – 1.241ln(C). The research implicated that the increasing value of those factors was approaching the suitable condition for A. marina. Thus, instead of increasing the phenol concentration, the parameters negatively effect the secondary metabolite. The result showed that increasing temperature, DO, and content of P and C reduced the stress in A. marina and reduce total phenol content. This suggests that low temperature, DO, P and C concentration provides more potential of phenolic products from A. marina. 
Diversity and Distribution of Bracket Fungi in Mt. Kilakiron, Bukidnon, Philippines Cababan, Mc Arthur Lequin; Jaranilla, Aprille Kake Ora; Bastatas, Mariane Catayas; Salvane, Chazzel Feel Comonong; Toldo, Uzziel Campuso
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.24064

Abstract

Fungi have a lot of important function in the bionetwork resembling to decomposition and even nutrient cycling with respect to its symbiotic relationships with trees and other plants. This study aimed to assess the diversity and distribution of bracket fungi in Mt. Kilakiron, Portulin, Pangantucan, Bukidnon. Thus, floristic survey was conducted in one of the mountain of Mindanao to provide information as to what and how many species of bracket fungi are present in Mt. Kilakioron, Bukidnon, Philippines.  40 total species belonging to 17 genera and 5 family was collected and assessed. 12 individuals of Trametes versicolor were observe which favors dwellings with low temperature and high altitude are. Additionally, study revealed that most types of bracket fungi initiate in the site are decomposers and saprophytic in nature which contributes a lot in the process of putrefaction. The species of bracket fungi in Mt. Kilakiron clearly shows abundant and provides a large contribution to the ecosystem having high Shannon diversity index. The significance of each environmental factor is discussed in relationship to the ecology of the species. Bracket fungi are reliant on the climatic and conservational settings which makes them as excellent natural pointers of ecosystem in the forests of Mindanao Island, Philippines.
The Antioxidant Content and Activity of Various Plant Organs of Kitolod (Isotoma longiflora) Egarani, Grahita Rezhi; Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23888

Abstract

Antioxidants play a crucial role in human health owing to their ability to neutralize oxidative reactions. Kitolod (Isotomalongiflora) is one of the medicinal plants that can be used as a source of antioxidant. The aim of study wasto compare the content of antioxidant compounds (flavonoids, phenolics, chlorophylls, carotenoids, and ascorbic acid) in various organs of the kitolod plant. The plant organs studied includingroots, stems, leaves, flowers, and fruits. The extraction of each plant organ wasconducted by maceration technique using ethanol as a solvent at the room temperature for 48 hours. The analysis of total flavonoids, phenolics, and ascorbic acid was performed by colorimetry method using AlCl3, Folin, and sulfosalicyclic acid reagent respectively, while chlorophylls and carotenoids was determined by direct colorimetry method with methanol as a solvent. The antioxidant activity of various plant organs of kitolod was analyzed using the DPPH method. The highest total flavonoid, phenolic, chlorophyll, and carotenoid compounds werefound in leaves, respectively by 10.48, 1.46, 7.25, and 56.98 ppm. The highest ascorbic acid content and antioxidant activity were obtained fromfruits.The research findings provide new and important information aboutthe contents and antioxidant activity of the secondary metabolites (flavonoid, phenolic, chlorophyll, carotenoid, and ascorbic acid) in each organ of kitolod plant. The information from the results of this study can be used to increase the medicinal value ofkitolod plants.
Isolation of Potential Plant Growth Promoting Rhizobacteria (PGPR) from Cassava (Manihot esculenta) Rhizosphere Soil Safriani, Suri Raihan; Fitri, Lenni; Ismail, Yulia Sari
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25905

Abstract

Cassava is the third most important calorie source in tropical area after rice and corn. The microorganism associated with cassava roots may be potent and useful for application to promote plant growth. Plant growth promoting rhizobacteria  is a group of benefical bacteria that live in rhizosphere. The aim of this study was to isolate and to identify the potential Plant Growth Promoting Rhizobacteria (PGPR) from cassava rhizosphere soil. The study consisted of isolation and identification of bacteria based on morphological and biochemical characters, hypersensitive reaction test, the ability to solubilize potassium and phosphate, and the ability to inhibit the growth of pathogen Sclerotium rolfsii. A total of nine bacteria isolates were succesfully isolated from Cassava rhizosphere soil. Those isolates suspected as Micrococcus sp.1, Micrococcus sp.2, Micrococcus sp.3, Micrococcus sp.4, Micrococcus sp.5, Micrococcus sp.6, Neisseria sp.1, Neisseria sp.2 and Bacillus sp. All nine isolates did not show hypersensitivity reactions. Only Neisseria sp.1 and Neisseria sp. 2 were able to solubilize potassium and phosphate. All isolates were able to inhibit the growth of S. rolfsii. The highest inhibition was done by Micrococcus sp.4 (51.46 %). The physical and chemical properties of cassava rhizosphere soil affected the type of bacteria found in this study. The result confirmed that the potential isolates obtained from cassava rhizosphere soil can be plant growth promoters. The present study suggested that PGPR isolates might have potential in future field applications as plant growth promoters or biocontrol agents.
Science Web-Module Integrated with Tannery Waste as Local Potential to Improve Students’ Problem Solving Miswami, Atznain Iqma; Nurcahyo, Heru
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24356

Abstract

Problem solving skills are very important skill students must owned. The low score of students’ problem solving skills becomes a serious problem in the 21st century teaching and learning. The development of science web-based module integrated with tannery waste as local potential became a research that must be done. This research examined the appropriateness and the effectiveness of a science web-based module integrated with tannery waste as local potential in improving students’ problem solving skills. The Four-D research and development model was employed, consisting of define, design, develop, and disseminate. This research was conducted in the form of pretest posttest control group design. The appropriateness analysis conducted by content expert, teaching media expert, and science teacher indicating that the product was categorized very good. The effectiveness of the learning was tested using the independent sample t test with sig. value set at 0.000. The test showed that the science teaching done using the science web-based module was effective in improving students’ problem solving skills in SMP Negeri 1 Wungu. The product resulted from this study is expected to overcome the teaching and learning problems associated with the low score of students' problem-solving skills. Moreover, it can provide a real experience and independence to students in using the science web-based module to study.
The Improvement of Mini Tuber Production of Granola Potato Cultivar in Aeroponics System Sugiyono, Sugiyono; Prayoga, Lucky; Proklamasiningsih, Elly; Faozi, Khavid; Prasetyo, Rendie
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.27714

Abstract

Potato is an important food crop in both developed and developing countries and it is the fourth most important food crop after wheat, maize, and rice. Potato production in Indonesia has decreased in recent years, mainly due to the use of low quality tuber seed. The objective of this research was to determine the effect of plant materials, types of nutrients, and planting density on mini tuber formation, as well as to determine the best aeroponics conditions for mini tuber formation of Granola cultivar of potato. This research has been carried out experimentally using a Split-split Plot Design. The main plot was planting materials which consisted of plantlet and knol. The subplot was the type of nutrition media which consisted of Farran nutrition, Otazu nutrition, and AB-commercial mix. The sub-sub-plot was planting density which consisted of 50, 75, and 100 plants/m2. The research results showed that knol planted in aeroponics system on AB-commercial mix at a planting density of 75 plants/m2 resulted in the best mini-tuber production. In this study, for the first time three important factors for potato mini-tuber production have thoroughly been investigated in Indonesia. This finding will be used by Kledung Horticultural Seed Garden, Temanggung, for mass production of high-quality mini-tuber seed, which in turn will provide sufficient tuber seed for the farmer in its surrounding areas. By increasing the production of high-quality tuber seed, it is expected that it will contribute to the improvement of potato productivity and increase Indonesian potato production.
Nutritional Content of Bone Flour Made from Plecos Fish Pterygoplichthys pardalis from the Ciliwung River, Indonesia Putri, Handhini Dwi; Elfidasari, Dewi; Haninah, Haninah; Sugoro, Irawan
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23881

Abstract

Pterygoplichthys pardalis bones that are thrown into the river is one source of pollutants in the Ciliwung River. P. pardalis bones have a high calcium content, they can be used as an alternative raw material for flour. The purpose of this study was to analyze the nutritional content of P. pardalis bone flour from the Ciliwung River. The research method used was a proximate analysis consisting of moisture, ash, fat, protein, and carbohydrates content analyses. Proximate analysis results showed that the P. pardalis bone plus head flour contained 3% moisture, 35% ash, 34% protein, 23% fat, and 5% carbohydrate. P. pardalis body bone flour contained 4% water, 37% ash, 37% protein, 19% fat, and 3% carbohydrate. Based on the proximate analysis results, P. pardalis bone flours did not met the national standard of SNI 2013 fish flour for quality I, II, and III. Until now, there has been no research that explain the nutritional content of bone flour of P. pardalis from Ciliwung River. This research provides information on the nutritional content of P. pardalis flour. Data obtained from the results of this study can be the basis for consideration in utilizing and processing P.pardalis from the Jakarta Ciliwung River.
The Cytogenetic Effects on Peripheral Blood Lymphocytes in Cancer Patients After Radiation Therapy: Chromosome Aberrations and Micronuclei Lusiyanti, Yanti; Surniyantoro, Harry Nugroho Eko; Rahajeng, Nastiti; Suvifan, Viria Agesti; Purnami, Sofiati; Choridah, Lina
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.25264

Abstract

Individual responses to radiotherapy are often observed whether or not regimes with identical treatments were applied. Patient-related factors, the therapeutic process, and therefore the intrinsic factors of individual radiosensitivity are considered to be causing the variability of side effects. A preliminary evaluation was done on cytogenetic biomarkers found in cancer patients. The purpose of this present study was to assess the individual response of patients with cancers after radiation therapy. The sample obtained from 11 patients with different types of cancer as a case group and 12 people as a control group from a healthy volunteer. Blood samples were stimulated by an in vitro culture using phytohemagglutinin, and the cultures were assessed by using the Dicentric and Cytokinesis- Block Micronucleus (CBMN-) assay. These two methods were compared. The results showed that the overall dicentric chromosome and micronuclei in binucleate cells (MN/BNC) have a significantly higher frequency in the breast, head, and neck compared to extremity cancer. A high frequency of micronuclei in lymphocyte patients was seen after radiotherapy treatment but relatively not much higher compared to the range of micronuclei backgrounds in healthy people The CBMN is the most effective assay for evaluation of the cytogenetic studies in cancer patients because it is more radiosensitive to study individual responses. By evaluating the effects of radiotherapy based on DNA damage, the severity of radiation exposure can be studied. This study can be useful for researchers and related stakeholders in the application of radiotherapy.
Candida Species Distribution of Clinical Specimens in Banda Aceh, Indonesia Suhartono, Suhartono; Mahdani, Wilda; Masthura, Aderiana; Rusmana, Iman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23758

Abstract

Candidiasis has become increasingly widespread in the community alongside with the developing resistance of Candida sp. to some antifungals. A prevalence study in the present research is required to surveil the distribution of Candida-related infections to administrate the appropriate antifungal treatments. The objective of this research was to determine the species distribution of candidiasis with their antifungal susceptibility isolated from clinical specimens at the Zainoel Abidin Hospital (ZAH) Banda Aceh, Indonesia. The clinical specimens were from inpatients and outpatients in the hospital during January to June 2019. The identification of Candida species and antifungal sensitivity assay were conducted by using VITEK® 2 Compact. Of a total of 68 isolates, there were six species of Candida with the highest species prevalence was Candida tropicalis (52.94%). Additionally, the highest prevalence of candidiasis came from urine specimen (54.41%) and mostly from inpatients in the internal medicine unit (54.41%). Candidiasis occurred predominantly in men (58.82%) and during adulthood (55.88%). The antibiogram of Candida sp. shows a high percentage of sensitivity towards some antifungals including fluconazole (100%), voriconazole (100%), caspofungin (100%), micafungin (100%), amphotericin B (99.5%), and flucytosine (100%). From this result, it can be concluded that candidiasis cases in the ZAH Banda Aceh, Indonesia has prevalently occurred with the few antifungal therapies for candidiasis of were still effective empirically and definitively. This is an initial study of Candida prevalence within different clinical samples in Banda Aceh and the study is expected to be a basis for prevention and control of Candida-related infections in the area.

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