cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Dr. Ir. Dyah Rini Indriyanti
Contact Email
biosaintifika@mail.unnes.ac.id
Phone
-
Journal Mail Official
biosaintifika@mail.unnes.ac.id
Editorial Address
-
Location
Kota semarang,
Jawa tengah
INDONESIA
Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 41   42   43   44   45 
Blood Hematological and Biochemical Parameters of Osteochilus vittatus with Spirulina platensis Supplementation in Biofloc System Rijal, Muhammad Azharul; Susanto, Susanto; Simanjuntak, Sorta Basar Ida; Hernayanti, Hernayanti; Sukardi, Purnama
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.20936

Abstract

Spirulina platensis is a microalgae that contains nutrients such us iron and phycocianin higher than other microalgae. Research on suplementation of S. platensis in Osteochilus vittatus cultured in biofloc system has never been done. The aim of this study was to determine the blood hematological and biochemical parameters of O. vittatus fed with S. platensis supplementation and maintained in biofloc system. The study was conducted experimentally with Completely Randomized Design, four treatments and five replications. O. vittatus were fed with S. platensis level 0, 2, 4, and 6 g.kg-1 for 56 days. Blood hematological and biochemical parameters were measured on days 0 and 56. Values of those parameters were then analyzed using ANOVA with a confidence level of 95%. The result showed that the highest red blood cells, white blood cells, and hemoglobin counts were 1.57x106­­­­ cell.mm-3, 2.37x105 cell.mm-3, and 6.77 g.dl-1 respectivley, while the highest hematocrit value was 17.5 %. The highest total protein, albumin, and globulin in blood were 7.96 g.dL-1, 4.31 g.dL-1, and 3.79 g.dL-1 respectively, and the best for ratio A/G was 1.14. S. platensis suplementation level of 4g.kg-1 feed was the most optimum level (P<0.05). Cultivation of fish with S. platensis supplementation in feed can increase fish health indicated by the hematological and biochemical parameters of the blood and the fish maintenance in biofloc systems can improve water quality. The benefit of this research is to make a good condition for cultivation and efficiently used not only water but also feed.
Antibacterial Activity and Flavonoids Content of Artemisia cina Berg. ex Poljakov Ethyl Acetate Extracts Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok; Herawati, Maria Marina; Sukmana, Andreas Binar Aji
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.27665

Abstract

The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial  potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent. 
Larvicidal Activity of Brugmansia candida against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae) Himmi, Setiawan Khoirul; Tarmadi, Didi; Meisyara, Dita; Fajar, Anugerah; Kartika, Titik; Guswenrivo, Ikhsan; Yusuf, Sulaeman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24634

Abstract

Mosquitoes are well known as vectors of hazardous diseases for human. Plant extracts can be used as an alternative for larval control due to they are a rich source of bioactive chemicals and safe for the environment. The present study investigated the larvicidal activity of crude extracts derived from leaf and flower of Brugmansia candida against the second larval instar of Aedes aegypti and Culex quinquefasciatus. The larval mortality was observed at 24 and 48 h exposure of both leaf and flower extracts, at the concentration of 100, 250, 500, and 1000 ppm. The 24 h exposure of both extracts at the concentration of 500 and 1000 ppm resulted in larval mortality rates were significantly lower than those of 48 h exposure. However, the mortality rate was not significantly different at the lower concentrations of crude extracts. The results also suggested that there was no significant difference in the larvicidal effect between leaf and flower extracts at 24 and 48 h exposure for all concentrations. The LC50 values at 48 h exposure for leaf extract were 789 and 791 ppm for Ae. aegypti and C. quinquefasciatus, respectively, whereas for flower extract were 772 and 780 ppm for Ae. aegypti and C. quinquefasciatus, respectively. Overall, B. candida showed larvicidal activity against Ae. aegypti and C. quinquefasciatus. This research contributes to new finding regarding the larvicidal activity of B. candida. This finding also supports the next study to develop B. candida as an alternative source for larval control agent.
Productivity of Arrowroots and Taro Grown Under Superior Teak Clones with Several Levels of Stand Density Prehaten, Daryono; Hardiwinoto, Suryo; Na’iem, Mohammad; Supriyo, Haryono; Widiyatno, Widiyatno; Rodiana, Dian
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.26428

Abstract

Perum Perhutani has an important role in providing food and wood for people. Diversity and diversification of food will reduce the need for one type of food, namely rice as a staple food. Some tuber and rhizome are source of alternative foodstuffs, such as taro and arrowroot. This study aimed to determine the suitability and productivity of arrowroot and taro planted under old superior teak clones with several levels of stand density. Arrowroot and taro were planted under 14-year-old teak stands with 4 levels of density. Both plants were planted in the form of an array, measuring of 3m x 15m, with a spacing of 75cm x 75cm between plants. They were arranged in Randomized Completely with Block Design (RCBD) placed in 4 blocks of observation as replications.  The results showed that under superior teak clone had the potential to be planted with arrowroot and taro. Teak stand density influenced significantly some characteristics of arrowroot (leaves number, leaves area, stem height, stem diameter, root length) and taro (stem diameter, tuber diameter). Arrowroot productivity per hectare increased with low density of teak stands, accounted for 55, 59, 80, and 88 kg respectively. Meanwhile, taro productivity from very high to low teak density were 365, 301, 523, and 426 kg/ha. The novelty of this study is that there is no record of intercropping studies on old superior teak clones, so this is among the first studies. The benefit of from this research result, it could be employed by Perhutani to support the Indonesian government in the national food security program
Identification of Gamma-Amino Butyric Acid Isolates Lactic Acid Bacteria Results from The Isolation of Rusip Prayitno, S Feta Avila; Suprihadi, Agung; Kusdiyantini, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24995

Abstract

Gamma-aminobutyric acid (GABA) is a non-protein amino acid produced by glutamate decarboxylation by the enzyme glutamate decarboxylase and it is widely distributed in plants, animals and microorganisms. GABA-producing microorganisms including Lactic Acid Bacteria (LAB) which play a role in the fermentation process of food such as rusip. Rusip is a preserved food of marine fish made from raw anchovy of Bangka-Belitung. The purpose of this study was to obtain LAB isolates through the process of isolation from rusip and identify the presence of GABA. Isolation of LAB was carried out by the pour method on MRS agar and NA medium. The growing colonies were then characterized based on observations of colony morphology, Gram staining, catalase test, motility test, and fermentation type test. LAB isolates obtained were selected to obtain isolates which are capable to produce GABA using the Thin Layer Chromatography (TLC) method. The results of isolation from rusip and inoculation on MRS agar medium obtained, three LAB isolates namely RSP-A1, RSP-A2, and RSP-A3. Their characteristics are Gram positive bacteria, negative catalase test, negative motility test, and belong to homofermentative and heterofermentative bacteria groups. They were also able to produce GABA. Research on GABA from isolates isolated from rusip is a new thing in the world of GABA research. This research provides information that is beneficial to produce GABA easily, cheaply, and efficiently.
In Situ Bioremediation Strategies for the Recovery of Mercury-contaminated Land in Abandoned Traditional Gold Mines in Indonesia Winardi, Winardi; Haryono, Eko; Sudrajat, Sudrajat; Soetarto, Endang Sutariningsih
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25229

Abstract

Traditional gold mining activities release mercury into the environment, creating a major concern for the Indonesian governments today. In situ bioremediation, which draws on the activities of indigenous soil bacteria for the recovery of mercury-contaminated land, has never been conducted intensively in the country. This research set out to determine the most efficient in situ bioremediation strategy for this purpose. It took place in Mandor Village, Landak Regency, Kalimantan Barat-Indonesia. During the experiment, four groups of sampling plots were made into triplicate and given various treatments: a. nutrient addition, b. aeration, c. pH neutralization, and d. without nutrient addition and aeration as a control. pH neutralization was conducted in all sampling plots by adding lime until soil pH of ±7 was achieved. The experiment was performed during both rainy and dry seasons to determine the influence of seasonal weather. Total mercury levels of each plot were measured on day 0, 30, 60, 90, and 120, and the effects of treatments and time on mercury depletion were analyzed by two-way ANOVA (P<0.05), followed by a post hoc test to identify the best treatment and optimum time for in situ bioremediation. The results showed that the best time to conduct this bioremediation was in the rainy season by applying nutrient addition and aeration for 90 days on soils with neutral pH; these stimulations could remove ±89.6% of the mercury. This bioremediation technique is a novel technological approach in land recovery that local governments can adopt to restore soils contaminated with mercury from traditional gold mining.  
Morphological Variation of Blue Panchax (Aplocheilus panchax) Lives in Different Habitat Assessed Using Truss Morphometric Mustikasari, Diah; Suryaningsih, Suhestri; Nuryanto, Agus
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.26593

Abstract

Blue panchax (Aplocheilus panchax Hamilton, 1822) lives in broad ranges of habitat from open waters to closed waters, including at ex-tin mining pits in Bangka Island, Indonesia. Variable habitats might cause morphological variations due to different ecological factors. This study aimed to assess the morphological variation of blue panchax collected from different habitats using truss morphometric characters. Fish samples (70 individuals) were collected from abandoned ex-tin mining pits of different ages and a river in Bangka Island. Twenty-nine truss characters were analyzed using the Kruskal-Wallis test and post hoc with Dunn’s test . The results showed that almost all of the body parts of blue panchax found in ex-tin mining pits and rivers were significantly different (p-value < 0.05), except some truss characters of caudal peduncle. The post hoc of Dunn’s test showed a positive correlation between habitats and truss characters (adj. sig < 0.05). Some truss characters of killifish were different between ex-tin mining pits with different ages chronosequence, and some other characters were different between pits and river.  This study provides the first data about the morphological variation of blue panchax in ex-tin mining pits of different ages. The data is valuable as a scientific basis of further utilization of ex-tin mining pits in the areas.
Potential of Red Okra Extract (Abelmoschus esculentus L. Moench) to Restore Kidney Damage due to Sodium Nitrite Wahyuningsih, Sri Puji Astuti; Fachrisa, Amalia; Nisa’, Nabilatun; Kusuma, Baskara Wiku Adi; Shoukat, Nadia; Ahmar, Rasyidah Fauzia; Alifiyah, Na'ilah Insani
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.28141

Abstract

Sodium nitrite (NaNO2) found in vegetables, drinking water, and cured meats, can damage tissue because it is an oxidant. Plant phytochemicals such as quercetin are antioxidants. This study aimed to determine the potential of red okra pods ethanol extract (ROE) to repair kidney damage in mice (Mus musculus) induced by NaNO2. The red okra pods were extracted three times with saturated ethanol. The experiment used 36 male BALB/c mice aged 6-8 weeks and body weight of about 28 g. There are six research groups, namely, normal control, negative control (exposure to NaNO2 50 mg/kg BW), treatment of exposure to NaNO2 and administration of ROE at doses of 25, 50, 75, and 100 mg/kg BW. Sodium nitrite and ROE were given daily for 23 days by gavage. On day 24, the serum was isolated. Blood urea nitrogen (BUN) and creatinine (Cre) levels are measured to assess kidney function, as well as measuring the oxidant malondialdehyde (MDA) and the antioxidant enzyme of superoxide dismutase (SOD). The kidneys were made histological preparations and analyzed on the proximal convoluted tubule (PCT).  All data were statistically analyzed (α=0.05). This study indicated that the administration of ROE at a 100 mg/kg BW dose is the most optimal in repairing damage to the PCT with increased normal cells and reduced necrosis. Besides, it degraded BUN, Cre, and MDA levels in the serum of mice exposed to NaNO2 compared to the other treatments. All doses of ROE promoted the SOD level. ROE restore kidney tissue, especially on PCT to normal. Kidney damage due to exposure to NaNO2 preservatives can be reduced by administering ROE. ROE prevents kidney damage through an increase in antioxidant enzymes. ROE can be used as a food ingredient as a source of antioxidants, thereby reducing the impact of oxidant compounds.
Potential of Ecology based Agrotourism for Agricultural Education in Besur Village, Lamongan Regency Ifandi, Slamet; Rahma, Yustika Aulia
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25886

Abstract

Currently, agriculture has prospective potential to be developed as a tourist attraction, one of which is agricultural tourism with an ecological perspective. The objective of the research was to examine the potential of ecology-based agrotourism as a means for agricultural education in Besur Village, Lamongan Regency. The research applied a quantitative descriptive approach in the form of scoring, weighting, and ranking of the categories being assessed. The research data consisted of primary and secondary data. The data was collected using direct observation and in-depth interviews. The data analysis was carried out using for the feasibility of  agrotourism area. The results showed that the area of Besur Village was classified as suitable (potential) with zone I that had the highest KKA value (2.6) as the object of the agrotourism. Potential ecological aspects obtained analysis 36 species of food producing plants. The highest Importance Value Index (IVI) of plant species was  recorded for Oryza sativa spp. (83.76%) and Zea mays L. (27.11%). Analysis of the physico-chemical properties of soil resulted in moderate or sufficient fertility. Analysis of public perceptions obtained was 4.08 and classified as good for the development of agrotourism in Besur Village. The novelty of this research was to obtain of the potential for agrotourism management in Besur Village through an ecological approach based on the concept of Healthy Plant Management.
Biodecolorization of Anthraquinone and Azo Dyes by Newly Isolated Indonesian White-Rot Fungi Ramadhan, Kharisma Panji; Anita, Sita Heris; Oktaviani, Maulida; Sari, Fahriya Puspita; Budi Laksana, Raden Permana; Nurhayat, Oktan Dwi; Yuli Yanto, Dede Heri
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.26148

Abstract

Water pollution by dyes represents from dyestuff industry becomes an environmental concern. Finding new isolates capable of decolorizing these dyes is important. The study aimed to assess the new isolates of white-rot fungi (WRF) as decolorizing agent of anthraquinone and azo dyes. Decolorization assay were conducted in Agar plates and liquid medium. During the decolorization, laccase activities produced by the fungal strains were analyzed. Identification of the fungal strains were investigated using molecular DNA analysis. The results showed that isolates M3, H18, and GP1 were able to decolorize anthraquinone and azo dyes in Agar and liquid medium. Based on DNA analysis, isolates M3, H18, and GP1 have the similarity to Trametes sanguinea, Trametes polyzona, and Neofomitella guangxiensis, respectively. Among the fungi, T. polyzona H18 exhibited high decolorization ability (70–90%) to the dyes (100 mg/L) after 96-hours incubation. Laccase activity was fluctuated during the reactions with tendency to increase at the beginning until its peak, then decreased at the end of incubation. This study demonstrated the potential of the new isolates from Indonesia to decolorize anthraquinone and azo dyes. The results of the study can provide an alteranative for bioremediation agents of contaminated water by synthetic dyes.

Page 43 of 107 | Total Record : 1068

 41   42   43   44   45 

Filter by Year

2009 2023


Reset
Filter By Issues
All Issue Vol 15, No 3 (2023): December 2023 Vol 15, No 2 (2023): August 2023 Vol 15, No 1 (2023): April 2023 Vol 14, No 3 (2022): Desember 2022 Vol 14, No 2 (2022): August 2022 Vol 14, No 1 (2022): April 2022 Vol 13, No 3 (2021): December 2021 Vol 13, No 2 (2021): August 2021 Vol 13, No 1 (2021): April 2021 Vol 12, No 3 (2020): December 2020 Vol 12, No 2 (2020): August 2020 Vol 12, No 1 (2020): April 2020 Article-in-Press Vol 12, No 1 (2020): April 2020 Vol 11, No 3 (2019): December 2019 Vol 11, No 2 (2019): August 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 3 (2019): Article-in-Press Vol 10, No 3 (2018): December 2018 Vol 10, No 3 (2018): December 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 1 (2018): April 2018 Vol 10, No 1 (2018): April 2018 Vol 9, No 3 (2017): December 2017 Vol 9, No 3 (2017): December 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 1 (2017): April 2017 Vol 9, No 1 (2017): April 2017 Vol 8, No 3 (2016): December 2016 Vol 8, No 3 (2016): December 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 1 (2016): March 2016 Vol 8, No 1 (2016): March 2016 Vol 7, No 2 (2015): September 2015 Vol 7, No 2 (2015): September 2015 Vol 7, No 1 (2015): March 2015 Vol 7, No 1 (2015): March 2015 Vol 6, No 2 (2014): September 2014 Vol 6, No 2 (2014): September 2014 Vol 6, No 1 (2014): March 2014 Vol 6, No 1 (2014): March 2014 Vol 5, No 2 (2013): September 2013 Vol 5, No 2 (2013): September 2013 Vol 5, No 1 (2013): March 2013 Vol 5, No 1 (2013): March 2013 Vol 4, No 2 (2012): September 2012 Vol 4, No 2 (2012): September 2012 Vol 4, No 1 (2012): March 2012 Vol 4, No 1 (2012): March 2012 Vol 2, No 2 (2010): September 2010 Vol 2, No 2 (2010): September 2010 Vol 2, No 1 (2010): March 2010 Vol 2, No 1 (2010): March 2010 Vol 1, No 1 (2009): March 2009 Vol 1, No 1 (2009): March 2009 More Issue