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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Characteristics and Antioxidant Activity of Kebar Grass (Biophytum petersianum) Extract Aminudin, Aminudin; Andarwulan, Nuri; Palupi, Nurheni Sri; Arifiantini, Iis
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23820

Abstract

Kebar grass (Biophytum petersianum Klotzsch.) has long been known by the locals of West Papua as medicinal plant that improves fertility. The objectives of this research were to identify and determine the antioxidant activity of kebar grass crude extract through conventional method and to analyze for its antioxidant activity. Extraction method used was conventional method, performed by mixing dry kebar grass with water and boiled until the volume shrunk to 1/3 of initial volume. Analized parameters were crude extract yield, qualitative phytochemical, total phenol and antioxidant activity. Research result showed extract crude yield value of 18.32±0.01%. Qualitative phytochemical contents were dominated by flavonoid, tannin, and saponin. Total phenolic content (TPC) of crude extract was 147±1.24 mgGAE/gdw (0.688±0.01 mgGAE/gfw). Crude extract antioxidant parameter was measured as the value of inhibition, AEAC, TEAC, and IC50 where each value respectively was as followed 42.0±0.047%, 69.93±2.19 mgAE/gdw (8.30±0.26 mgAE/gfw), 65.70±1.54 mgTE/gdw (7.80±0.18 mgTE/gfw) and 0.129±0.003 mgdw (257.75±4.90 mg/L). The contribution of kebar grass TPC content to antioxidant activity is 47.38% (equal to ascorbic acid) and 44.51% (equal to trolox) which means that almost half of kebar grass crude extract’s phenolic compound act as antioxidant. The results of this research can show that the kebar grass has potential as a source of multiple antioxidants, which acts as an analogue of vitamin C and vitamin E.  The antioxidant activity of the kebar grass extract can be the scientific basis for the use of kebar grass as a medicinal plant for infertility problems (curative) and as a functional food ingredient for antioxidant sources that prevent a decrease in reproductive performance (preventive).
Effect of Drying Techniques on Nutrition Content and Gross Energy of Nuabosi Cassava (Manihot esculenta) Flour Priska, Melania; Peni, Natalia; Carvallo, Ludovicus; Wae, Veronika P. S. M.
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24184

Abstract

This reseach aimed to determine the proper cassava drying technique to produce suitable flour properties for food product applications. The method used was proximate analysis and calculation of Gross Energy (GE) value. Furthermore, the results of the study were analyzed using the Two Way ANOVA statistical test followed by the real difference test using the Tukey analysis. Results of the analysis showed that there was no significant difference between the two drying techniques (α>0.05). To get good quality flour in the application of food products made from Nuabosi cassava flour is to use oven drying and sun drying techniques. The novelty of this study is that there has been no previous research on the quality of Nuabosi cassava flour using oven drying and sun drying techniques. The benefit of this research is to support the government in the food diversification program on foods made from Nuabosi cassava flour, where Nuabosi cassava is one of the leading types of food in Ende district.
Microbial Succession and Chemical Characteristics in Fermentation of Ambonese Arrack (Sopi), Traditional Beverage from Maluku Mahulette, Ferymon; Astuti, Dea Indriani
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23791

Abstract

Ambonese arrack is one of the traditional fermented beverage product in Maluku, Indonesia. The microbiological research of this beverage that is processed using coconut sap as raw material has never been done before. The research aimed to analyze the microbial succession and chemical characteristics during fermentation of Ambonese arrack. The sample of coconut sap was taken from traditional producer in Mahia village, Ambon. The dominant microbes in the fermentation of Ambonese arrack were Pichia polymorpha and Kloeckera javanica. The highest numbers of these two microbes was obtained after 15 hours fermentation (9.6 log Cfu/mL and 9.9 Cfu/mL, respectively). The sugar content decreased from 593.3 mg/L to 474.3 mg/L, whereas ethanol content increased from 0.0018 g/L to 0.0100 g/L. The pH value decreased from 4.70 to 3.10. The research has isolated indigenous microbes in Ambonese arrack fermentation which was considered as novelty. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various beverage products, especially Ambonese arrack. The results of this research can improve the quality of this fermentation product in the future.
Effects of Moringa oleifera Leaves Powder in Fish Feed Toward Growth Rate and Health of Colossoma macropomum Safrida, Safrida; Noviasyah, Nonong; Khairil, Khairil
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.22655

Abstract

Tambaqui (Colossoma macropomum) is one of freshwater fish that preferred by the community and also in high demand by fish farmers. It has a high selling value and easy to maintain but the growth is slow. One alternative that can be used to improve the fish growth is making rations with the addition of Moringa oleifera leaves. The aim of this study was to determine the effect of Moringa leaves powder on the growth rate and health of C. macropomum. This study used an experimental method with Completely Randomized Design (CRD) with 6 treatments and 4 replications. The data were analyzed using ANOVA Test in α =95%. The results showed that P3 with the addition of 30% Moringa leaves powder could increase the growth of Colossoma macropomum. An increase in fish body weight, width, and length was 40.1%, 21.6%, and 6.4% respectively.  Results of ocular, reflex, and defensive test showed that the fish were in healthy condition compared to their condition before acclimatization. The conclusions of this study is that the addition of Moringa leaves powder in an influential ration can increase the growth and maintain the health of C. macropomum. M. oleifera daily supplement can be used as a substitute for soy flour to promote growth and health of Colossoma macropomum in the seed phase. The addition of M. oleifera can be used as feed supplements to improve the growth and health of Colossoma macropomum.
Raw Secondary Metabolites of Trichoderma harzianum T10 in Tapioca Flour Towards Cucumber Damping-off Soesanto, Loekas; Ilahiyyah, Hidayatul; Mugiastuti, Endang; Manan, Abdul; Rostaman, Rostaman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24567

Abstract

Trichoderma harzianum is effective for controlling soil-borne pathogenic fungi and producing secondary metabolites. When applied in the field, the raw secondary metabolites are quickly decreased directly by sunlight. One strategy to avoid degradation is the use of tapioca fluor liquid formula for biological control agents. This research aimed to obtain the most effective concentration of tapioca flour in development of raw secondary metabolites of Trichoderma harzianum T10, its effect on damping-off and growth of cucumber. This research was carried out at the screen house and the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University from September 2017 up to January 2018. The study was conducted in two stages, i.e., in vitro and in planta. The in vitro stage used completely randomized design with five repetitions and five treatments consisted of T. harzianum T10 in Potato Dextrose Broth, and in 0.5; 1; 1.5; and 2% of tapioca flour media. In in planta, randomized block design was used with five repetitions and six treatments consisted of control, T. harzianum T10 in PDB, and in 0.5; 1; 1.5, and 2% of tapioca flour media. Variables observed were density of conidia, disease incubation period, disease incidence, AUDPC, maximum growth potential, germination ability, plant height, canopy fresh weight, root length, and fresh root weight. Result of the research showed that the highest conidial density (1.23 x 107 conidia mL-1) of T. harzianum T10 was found in 2% tapioca flour with an increase of 63.28% compared to the PDB. The tapioca flour of 1 and 2%, and PDB could suppress the disease incidence by 81.82%. The lowest AUDPC was at 2% tapioca flour. The raw secondary metabolites could not delay the incubation period significantly and increase cucumber plant growth. The novelty is the use of antagonistic fungi in terms of raw secondary metabolites and the discovery of tapioca flour with the right concentration to produce high conidia density and high raw secondary metabolites. The benefits are to find other cheaper ingredients in promoting antagonistic fungal growth and the use of antagonistic fungal bioactive compounds to control plant pathogen
Isolation, Screening and Identification of Plant Growth-Promoting Endophytic Bacteria from Theobroma cacao Simarmata, Rumella; Widowati, Tiwit; Dewi, Tirta Kumala; Lekatompessy, Sylvia J. R.; Antonius, Sarjiya
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.21280

Abstract

Cacao (Theobrama cacao) is one of the main plantation commodities in Indonesia which has an important role for the national economy. The low productivity of cacao plants in Indonesia is due to the condition of old plants, pests and diseases attacks which affect the quality of the fruit and decrease the plant’s productivity. The objective of this study was to isolate and identify of endophytic bacteria from stem, flower, leaf and fruit of T. cacao by using 16S rRNA gene as genetic marker. Twenty seven endophytic bacterial isolates were collected from local plantation in Yogyakarta area.  From this study, 8 endophytic bacterial strains exhibited the higher PGP traits. The isolates produced Indole Acetic Acid level by 0.3 to 5.21 ppm/hour. All of isolates had nitrogen fixation activity but have not phosphate solubilization activity. Among them, isolates CSDT 4 and CGKBH 4 showed promising potential as PGP bacteria. Based on the 16S rRNA gene sequences, those bacterial strains were identified as Brevibacillus brevis (CSDT 4) and Pantoea sp. (CGKBH 4). We propose that the B. brevis and Pantoea sp. which is reported for the first time for their PGP potential in cacao, exerts its beneficial effects on cacao crop through combined of activities. The potential PGP bacteria from the Cacao plant was used to make a specific bio-fertilizer formula for the Cacao plant, because of the different needs and condition that every plant requires.
Characterization of Ficus benjamina and Artocarpus heterophyllus Proteases as Potential Rennet Alternatives Sukmana, Andreas Binar Aji; Widyaningrum, Indy; Lani, Rischa Karmila; Kasmiyati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23516

Abstract

Rennet, a milk coagulant exhibiting proteolytic activity, is a crucial component in cheese industries. Its price and availability have discouraged the growth of some small scale cheese industries. Therefore, an alternative for rennet will be beneficial for the industries. Among other sources, plant proteases offer some advantages as rennet alternatives. This study aimed to investigate the potential of plant proteases obtained from the latex as potential rennet alternatives. A total of six plants from the genus Ficus and Artocarpus were screened for their proteolytic activity and milk coagulating ability. The screening indicated that all six tested plants displayed proteolytic activity at various levels, but only Ficus benjamina and Artocarpus heterophyllus produced a firm milk curd. Hence, both F. benjamina and A. heterophyllus were determined to be the most potential. Further characterizations suggested that F. benjamina and A. heterophyllus protease were optimum at pH 7.0 also at 50°C and 40°C, respectively. At their optimum conditions, both proteases exhibited a lower MCA/PA ratio than that of the rennet. This study contributed to scientific knowledge development by becoming the first to characterize the optimum conditions of F. benjamina and A. heterophyllus’ proteases, investigate their MCA/PA ratio, and compare their activity against commercial rennet. The examination of their potentials as rennet alternatives could benefit small cheese industries and the communities.
The Best Inoculation Technique Applied on Gyrinops versteegii Tree Trunk Wahyuni, Resti; Prihantini, Amalia Indah
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.25128

Abstract

Agarwood is a non-timber forest product having high economic value, however, its population in nature is getting decrease. Gyrinops versteegii, an agarwood-producing plant, has been listed in Appendix II CITES, therefore, exploitation of the agarwood in nature must be reduced. An effort to reduce the agarwood exploitation the nature is by agarwood cultivation including cultivation of agarwood-producing plant and agarwood inoculation. Some of agarwood inoculation methods are simpori and implant techniques. Simpori is a modified inoculation method using porous nail and Fusarium solani, meanwhile implant technique uses drill and pieces of wood that is soaked in F. solani spores. The present study aimed to determine the best inoculation technique applied to G. versteegii tree trunk at 4 to 5m height, comparing simpori and implant techniques. This study used completely randomizes design with two treatments (simpori and implant techniques). The result showed that the averages yield of production agarwood produced by simpori and implant techniques are 0.66 and 0.64%, respectively. Based on SNI 7631:2011, the visual quality of the produced agarwood belongs to kemedangan TG.C. In conclusion, both simpori and implant technique produced agarwood in similar quality and quantity at six months inoculation, however, simpori is more practical for application in the field. This study provides additional data about agarwood produced by simpori and implant technique and give the alternative methods for agarwood farmers producing their agarwood themselves.
Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria Prastiyanto, Muhammad Evy; Wardoyo, Fandhi Adi; Wilson, Wildiani; Darmawati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23600

Abstract

The multi-drug resistance (MDR) bacteria is a global health problem that causes high mortality every year. Therefore, novel antibacterial agents are needed from natural biological sources. This research aimed to investigate the antibacterial activities of various crude extracts of Averrhoa bilimbi against MDR bacteria. The antibacterial activity was calculated based on the use agar well diffusion assay and the minimum bactericidal concentration (MBC) using Mueller–Hinton broth in a microdilution method. Bacteria from wells were subcultured using inoculating loop onto a 5% sheep BAP. The best antibacterial activity, calculated as the most widely inhibitory zone and the smallest MBC values. The ethanolic extract showed antibacterial activity against the all MDR bacterial test in the agar well diffusion assay (10-14.5 mm inhibition diameter). The MBC of water extract against ESβL + CR Pseudomonas aeruginosa showed the best antibacterial activity (12.5 mg/mL). The fruit of bilimbi was shown to be potentially developed as antibacterial agents, especially for MDR strains. Further in vivo research and discovery of action mode are needed to shed light on their antibacterial effects. This study can provide new information about the benefits of bilimbi as a source of natural antibacterial againts MDR-bacteria
Anti-tumor Potential Ethnomedicinal Plants from Dayak Tribes in Central Kalimantan Neneng, Liswara; Pujirahadju, Asri; Ferayani, Ferayani; Widayat, Tri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23671

Abstract

Hornstedtia conica Ridl., Microsorum pustulatum Copel and Rourea fulgens Planch were medicinal plant used by  people of Dayak Tribes in Central Kalimantan for anti-tumor medicines. Although these plants has been widely used in traditional medicine, however, research and scientific evidence about their potential as anti-tumor are still very limited.  The research aims were to determine the qualitative content of phytochemical compounds and to study anti-tumor potential of these medicinal plants. This study used female Swiss Webster albino as a test animal.  Induction of tumor cell in mice used 7,12-Dimethylbenz [a] anthracene (DMBA), was done 10 times for 5 weeks.  Experiments were carried out used hexane extract from medicinal plants, every day for 7 weeks. The data obtained were analyzed descriptively. The results as follows: extracts of Hornstedtia conica Ridl., and Microsorum pustulatum Copel. contained alkaloids, while extract of Rourea fulgens Planch. contained flavonoids and saponins. All of these plant extracts were able to reduce the size and number of mice tumors. Hexane extract of H. conica was able to reduce the tumor size faster than another plants extracts. Mortality rate of mice in hexane extracts of H. conica and R. fulgens were zero- and in hexane extract of M. pustulatum it was 30%, meanwhile almost all mice in control group were died in days 15 of treatment. Based on this study, it can be concluded that hexane extract of H. conica and R. fulgens are potential as anti-tumor medicinal plants. These findings scientifically proved the potential of medicinal plant extracts as anti-tumor compounds.  H. conica is the most potential one for further development as alternative drug for inhibited of tumor growth.

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