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<![CDATA[Insect pollinators on Ananas comosus Linn. in pineapple cultivation hub at Tangkit Baru Village, Jambi Province: A preliminary study ]]>
<![CDATA[Ratna, Yuni]]>;
<![CDATA[Ristyadi, Dwi]]>;
<![CDATA[Arifah, Windyna]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.3732
<![CDATA[The flowers of Ananas comosus, pineapple, are generally self-sterile which means seeds will only develop if cross-pollination occurs. Therefore, insect pollinators play a crucial role in fostering and preserving genetic diversity in pineapple plants. Our research aimed as a preliminary study to gain insight into the key pollinators that play a significant role in the pollination of A. comosus in Tangkit Baru village, a prominent pineapple cultivation hub in Jambi Province. Sampling was done in 20 sampling points, five times at five-day intervals. Sampling was carried out using yellow sticky traps, sweep net, and direct observation. All the collected insects were identified to species level, then species diversity and evenness were assessed using Shannon’s diversity index (H’) and Pielou’s evenness index (J’). A total of 1167 individuals, representing 31 distinct species of insect pollinators belonging to 14 families were found in our sampling sites. We found four dominant species that could potentially serve as the key pollinators of A. comosus in our sampling site, i.e. Dolichoderus thoracicus, Pygophora enigma, Pygophora respondens, and Anoplolepis gracilipes. The diversity and evenness of insect pollinators in the sampling site were at a moderate level, with Shannon’s diversity index (H’) value of 2.0785 and pielou’s evenness index (J’) value of 0.6053. Habitat enhancement measures are needed to preserve and improve the existing pollinator diversity and evenness in the agroecosystem.]]>
<![CDATA[Formulation of Jakaba fertilizer from banana peel, pineapple peel, tea dregs for enhanced pak choi (Brassica rapa subsp. Chinensis) growth ]]>
<![CDATA[Muttaqin, Salwa Zainum]]>;
<![CDATA[Mamangkey, Jendri]]>;
<![CDATA[Silalahi, Marina]]>;
<![CDATA[Mendes, Lucas William]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.4470
<![CDATA[Excessive use of chemical fertilizers can lead to environmental degradation and increase the financial burden on farmers. As a sustainable alternative, liquid organic fertilizer (LOF) derived from Jakaba (also known as jamur keberuntungan abadi) was fermented using common organic wastes. This study aimed to evaluate the effects of different organic waste substrates on the characteristics of Jakaba-based LOF (JLOF) and the growth performance of pak choi (Brassica rapa subsp. Chinensis). A completely randomized design (CRD) was used, consisting of nine treatments with three replications each. The concentration variations of banana peel waste, pineapple peel, and tea grounds used in fermentation include 100 g, 200 g, and 300 g. Observations were conducted in three time intervals on days 0, 14, and 30. Parameters measured included the physical and biological characteristics of the LOF (i.e., pH and fungal growth), as well as plant height, leaf number, and fresh weight of pak choi. The data obtained were analyzed statistically using ANOVA analysis. The results showed that tea dregs provided the most favorable conditions for Jakaba growth and maintained the most stable pH. In contrast, pineapple and banana peels inhibited fungal growth, likely due to the presence of inhibitory compounds such as tannins and bromelain. Field application on pak choi indicated that JLOF derived from tea dregs promoted the best plant growth among all groups. These findings suggest that Jakaba-based LOF formulated with tea dregs holds promise as an effective organic fertilizer and may be further developed for commercial use.]]>
<![CDATA[The effect of guided inquiry with differentiated learning to enhance students' critical thinking skills ]]>
<![CDATA[Ningsih, Widia]]>;
<![CDATA[Sipayung, Dwi Yanti Cornelia]]>;
<![CDATA[Simatupang, Halim]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.4196
<![CDATA[This study was designed to determine the effect of guided inquiry model integrated with differentiated learning on students' critical thinking skills on respiratory system material in class VII junior high school. The background of this research is based on the low critical thinking skills of students in Indonesia, as well as the need for a learning approach that is able to respond to the diversity of learning styles, interests, and readiness of students in the classroom. The guided inquiry with differentiated learning model, employs a method of instruction that encourages students to solve problems through the application of analytical and critical thinking skills. The present study employs a quasi-experimental design, specifically pretest-postest control group design. The present study utilizes a critical thinking ability test instrument according to Ennis, comprising 10 essay questions. Hypothesis testing was carried out with a significance level of 0.05 with the help of SPSS version 26. The research results demonstrated a significant influence of the guided inquiry learning mode with differentiated learning on students' critical thinking abilities, as measured by the Mann-Whitney U test with Asymp scores. Sig (2-tailed) 0.000, indicating a significance value <0.05. In conclusion, guided inquiry with differentiated learning is effective to enhance students' critical thinking skills and can be an alternative adaptive and responsive science learning strategy in the Merdeka Curriculum.]]>
<![CDATA[The potential of pineapple peel waste fermentation as antibacterial and antioxidant agents ]]>
<![CDATA[Mawli, Rizka Efi]]>;
<![CDATA[Putri, Devi Anggraini]]>;
<![CDATA[Dewi, Maharani Putri]]>;
<![CDATA[Alfaini, Galuh Nuri]]>;
<![CDATA[Febriani, Afaf]]>;
<![CDATA[Anggraini, Dwi Aprilia]]>;
<![CDATA[Sutanto, Andri Kurniawan]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.3887
<![CDATA[The fermentation of pineapple peel waste can yield a product in bioactive compounds, including an eco-enzyme that serves as both an antibacterial and antioxidant agent. By utilizing the fermentation process, the extraction of phenolic compounds is enhanced through the action of degrading enzymes produced by microorganisms. This study aims to explore the potential of fermenting pineapple peel waste from with varying fermentation durations of one month (designated as EE-1) and three months (designated as EE-3) to assess their antibacterial and antioxidant properties. The method research is purely experimental methodology with several testing phases: the preparation of the eco-enzyme, a disc diffusion test for antibacterial activity, the determination of the minimum inhibitory concentration (MIC) using the resazurin microtiter assay (REMA), and the assessment of antioxidant activity through the DPPH test. The result showed that the eco-enzyme fermentation has a dark brown color for EE-1 and a light brown color for EE-3, with a pH range of 3 to 3.2 and a sour pineapple aroma. EE-3 exhibited the highest antibacterial and antioxidant activity. It demonstrated strong inhibition against Propionibacterium acnes at concentrations of 100and 102, with MIC values of 3.13 mg/mL for Staphylococcus aureus, 12.5 mg/mL for Staphylococcus epidermidis, and 6.25 mg/mL for P. acnes. The minimum bactericidal concentration (MBC) was found to be 25 mg/mL for all tested bacteria, while the antioxidant activity of EE-3 was indicated by an IC50 value of 1.95 mg/mL.]]>
<![CDATA[The superiority of Zymomonas mobilis in producing bioethanol from Coffea arabica peel using cellulolytic enzyme treatment ]]>
<![CDATA[Widyaningrum, Trianik]]>;
<![CDATA[Utami, Listiatie Budi]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.4482
<![CDATA[As petroleum supplies are being depleted, there is a growing need for bioethanol as an alternative energy source. With the aid of crude cellulose enzymes from Bacillus subtilis IFO 13719, coffee arabica peels can be used as one of the waste materials to manufacture bioethanol. Microorganisms that can convert sugar into bioethanol, specifically Saccharomyces cerevisiae and Zymomonas mobilis, are also necessary for the synthesis of bioethanol. The purpose of this study is to demonstrate Zymomonas mobilis's superiority in generating bioethanol from fermented Arabica coffee peels treated with the crude cellulose enzyme B. subtilis. The concentrations of the B. subtilis crude cellulase enzyme at 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% were all independent variables in the fully randomized experimental design. The amount of sugar and ethanol is the dependent variable. The DNS method was used to measure the sugar concentration, and an alcohol meter was used to determine the ethanol content. Data analysis using the SPSS 16 software to examine DMRT and one-way ANOVA. The results showed that the maximum sugar content (1.0 g/mL) was obtained with the cellulase enzyme B.subtilis 2.5 % and the highest ethanol content (4.46 %) was obtained with the crude enzyme B. subtilis concentration 10% and fermented using Z. mobilis.]]>
<![CDATA[Environmental literacy profile of Muhammadiyah junior high school students in Malang city: Intervention without having to consider factors! ]]>
<![CDATA[Husamah, H.]]>;
<![CDATA[Rahardjanto, Abdulkadir]]>;
<![CDATA[Hadi, Samsun]]>;
<![CDATA[Lestari, Nurdiyah]]>;
<![CDATA[Permana, Tutut Indria]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.4262
<![CDATA[Students of Muhammadiyah Junior High Schools and Muhammadiyah Islamic Junior High Schools need to have environmental literacy to practice environmentally friendly attitudes, become nature conservationists, and fulfill the school's role in serving education and the environment. However, there has been no mapping of EL profiles in Muhammadiyah Junior High Schools and Muhammadiyah Islamic Junior High Schools in Malang City. This study aims to analyze the EL profile of Muhammadiyah Junior High Schools and Muhammadiyah Islamic Junior High Schools in Malang City. Using a cross-sectional survey approach, EL data from 412 students in grades VII, VII, and IX in six Muhammadiyah schools were collected and analyzed based on gender, class, and parental education. The results showed that there were no significant differences in EL between male and female students, as well as between classes, and parental education. This study concludes that interventions or policies to improve environmental literacy can be applied universally, regardless of gender, grade level, or parental education.]]>
<![CDATA[Scientific literacy-based encyclopedia on the antiaging properties of acehnese spices: A research and development approach ]]>
<![CDATA[Fiyantara, Aljun]]>;
<![CDATA[Diningrat, Diky Setya]]>;
<![CDATA[Hasairin, Ashar]]>;
<![CDATA[Hartono, Adi]]>;
<![CDATA[Salleh, Rabeta Mohd]]>;
<![CDATA[Sari, Ayu Nirmala]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.3928
<![CDATA[The aim of this research is to produce an encyclopedia of Acehnese spice plants that serves as an anti-aging agent developed based on scientific literacy. The encyclopedia of Acehnese spice plants will be used as one of the learning media in the Ethnobotany course. The development procedure was carried out using the ADDIE method which includes analyzing student needs and characteristics, formulating learning objectives, formulating material items, preparing evaluation instruments, writing drafts of encyclopedia, expert validation, field trials, and printing production scripts. Ethnobotanical experts conducted expert validation. The results of the expert validation test stated that the encyclopedia of Acehnese spice plants with antiaging potential as a learning resource to improve student science literacy in ethnobotany courses followed the design and the material included in it was suitable for use as learning media. The field trial results showed that the science literacy-based encyclopedia was very attractive in design and effectively used as learning media.]]>
<![CDATA[Exploring the implementation of the merdeka curriculum in biology education: A qualitative study at an Indonesian upper secondary school ]]>
<![CDATA[Tarigan, Mhd. Rafi'i Ma'arif]]>;
<![CDATA[Azis, Suhartini]]>;
<![CDATA[Lase, Natalia Kristiani]]>;
<![CDATA[Tarwiyani, Tarwiyani]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.4490
<![CDATA[This study aims to describe the implementation of the Merdeka Curriculum in Biology learning at SMA Negeri 1 Sibolga and to analyze its supporting and inhibiting factors. The study was conducted in the odd semester of the 2024-2025 academic year using a descriptive qualitative approach. Data collection techniques included observation, interviews, and documentation. The results show that the implementation of the Merdeka Curriculum in Biology was carried out gradually. Teachers showed strong enthusiasm and initiative in understanding and applying curriculum principles such as differentiated learning and project-based learning. The school supported this effort by providing a Biology laboratory, digital learning resources, and internal training. However, several challenges emerged, including unequal teacher understanding of the curriculum, difficulties in preparing teaching materials aligned with the learning outcome, and limited practical training. Learning evaluation was conducted regularly through formative assessments and classroom observations. Routine reflection served as a crucial means of improving instruction. The study concludes that the success of curriculum implementation is strongly influenced by teacher readiness, school support, and active student engagement. Strengthening teacher capacity and inter-teacher collaboration is recommended as a strategic step to optimize curriculum implementation.]]>
<![CDATA[Characterization of marine bacteria isolates GaN 2 and RaN 2 from Papuma Coastal, Jember ]]>
<![CDATA[Wathon, Syubbanul]]>;
<![CDATA[Senjarini, Kartika]]>;
<![CDATA[Oktarianti, Rike]]>;
<![CDATA[Hasanah, Fitrianti Milhatul]]>;
<![CDATA[Oktavia, Melinda Dwi Putri]]>;
<![CDATA[Puspitasari, Endah]]>;
<![CDATA[Labes, Antje]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.4289
<![CDATA[The exploration of secondary metabolites for various human needs has now extended to aquatic environments. The fluctuating and dynamic nature of aquatic environments can stimulate marine bacteria to adapt by producing secondary metabolites. The diversity of marine bacteria has become increasingly promising as a target for obtaining various potential secondary metabolites. This study aims to isolate potential marine bacteria that produce secondary metabolites from the Papuma Beach area in Jember. Marine bacterial characterization can be carried out through morphological and molecular approaches. In this study, bacterial isolates were collected from marine algae at aquatic sites. Bacteria were isolated from the algae using the spread plate and streak plate methods on nutrient agar dissolved in artificial seawater as the medium until single colonies were obtained. Morphological characterization of the single colonies was conducted by observing colony shape and color, as well as performing Gram staining. Molecular characterization of selected single colonies was performed by amplifying the 16S rDNA as a bacterial molecular marker. The results of this study showed that marine bacteria that grew well and remained stable on artificial media were successfully isolated from green and red algae samples, labeled GaN 2 and RaN 2, respectively. The GaN 2 bacterial isolate was identified as Fictibacillus barbaricus strain SQ5-4, while the RaN 2 bacterial isolate was identified as Bacillus altitudinis strain 41KF2bT.26. According to previous studies, Fictibacillus barbaricus is known to have potential as a bioremediation agent, while Bacillus altitudinis has potential applications as an antitumor, antimicrobial, and antioxidant-producing agent.]]>
<![CDATA[In vitro germination of lemon cui (Citrus microcarpa Bunge) ]]>
<![CDATA[Pagalla, Devi Bunga]]>;
<![CDATA[Kandowangko, Novri Youla]]>;
<![CDATA[Ahmad, Jusna]]>;
<![CDATA[Febriyanti, Febriyanti]]>
<![CDATA[ Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sumatera Utara ]]>
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DOI: 10.30821/biolokus.v8i1.3952
<![CDATA[Lemon cui (Citrus microcarpa Bunge) is widely utilized by the people of Gorontalo as a cooking ingredient, traditional medicine, cleaning agent, and cosmetic ingredient. This diversity of uses indicates the potential for further development of lemon cui. The initial stage of development that can be undertaken is the mass propagation of high-quality lemon cui seedlings. This study aims to produce lemon cui seedlings using in vitro culture techniques through seed germination.The experimental design used in this study is a Completely Randomized Design (CRD) with four treatments, namely: A: MS0 (without the addition of plant growth regulators and young coconut water); B: MS + 50 mL young coconut water + 0.5 ppm 2,4-D; C: MS + 50 mL young coconut water + 1 ppm 2,4-D; and D: MS + 50 mL young coconut water + 1.5 ppm 2,4-D. The culture medium used was Murashige and Skoog (MS) supplemented with young coconut water and 2,4-dichlorophenoxyacetic acid (2,4-D). The study's results showed that using young coconut water and 2,4-D significantly accelerated the germination of lemon cui seeds. The best result was observed in treatment D, where the citrus seeds exhibited a maximum germination rate of 100%. In addition, the combination of young coconut water and 2,4-D is capable of inducing callus formation during the germination process. The combination of 50 mL of young coconut water and 1.5 ppm 2,4-D is the most effective concentration for inducing germination of lemon cui seeds and callus formation.]]>
