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Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.

Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.

Antibacterial Activity and Potential Of Natural Textile Dyes From Sea Water Bacteria Syaifudin, Andang; Sutrisno, Sutrisno
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 1 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i1.13446

This research aims to determine the potential of bacterial isolates from Marina beach seawater to be used as natural textile dyes and to test their antibacterial ability. The media used for culturing bacteria is a zobel synthesis medium with a mixture of seawater. The results of the isolation and extraction of color pigments obtained a red color with a characteristic wavelength of 535 nm and has a variety of chemical content results. Pigment immersion trials using 3 types of fabric, namely cotton, primisima and dobby fabric. The results of immersion with ethanolic solvent obtained optimum results for 12 hours and with the addition of mordant (Fe.H2O4S.7H2O), the color of the fabric is strong enough to withstand washing with detergent and sunlight. From the results of the study, data showed that the dyed primisima fabric gave a reduction or inhibition of the growth of E. coli bacteria by 9% while the inhibition of S. aureus bacteria was 116%. The pigment has the potential to be used as a dye for batik cloth with the provisions of optimizing to find the right reinforcement or mordant. This study has implications for the discovery of red pigment-producing bacteria from the isolation of seawater at Marina Semarang beach.

Antibacterial Activity and Potential Of Natural Textile Dyes From Sea Water Bacteria Syaifudin, Andang; Sutrisno, Sutrisno
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 1 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i1.13446

This research aims to determine the potential of bacterial isolates from Marina beach seawater to be used as natural textile dyes and to test their antibacterial ability. The media used for culturing bacteria is a zobel synthesis medium with a mixture of seawater. The results of the isolation and extraction of color pigments obtained a red color with a characteristic wavelength of 535 nm and has a variety of chemical content results. Pigment immersion trials using 3 types of fabric, namely cotton, primisima and dobby fabric. The results of immersion with ethanolic solvent obtained optimum results for 12 hours and with the addition of mordant (Fe.H2O4S.7H2O), the color of the fabric is strong enough to withstand washing with detergent and sunlight. From the results of the study, data showed that the dyed primisima fabric gave a reduction or inhibition of the growth of E. coli bacteria by 9% while the inhibition of S. aureus bacteria was 116%. The pigment has the potential to be used as a dye for batik cloth with the provisions of optimizing to find the right reinforcement or mordant. This study has implications for the discovery of red pigment-producing bacteria from the isolation of seawater at Marina Semarang beach.

Identification of Bird Species in Argowiloso Peak Mount Muria Ridlo, Muhammad; Setyawati, Siti Mukhlishoh; Hidayat, Saifullah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 1 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i1.13530

AbstractIndonesia is a country that has abundant flora and fauna. Mount Muria, Kudus Regency, Central Java has a high potential for bird biodiversity. The area which is a natural protected forest area, is very supportive for the existing bird habitat. However, this potential is still rarely known by the general public due to the lack of publications. The aims to find out and analyze the types of birds and to find out and analyze the condition of the bird habitat found in the Mount Muria area. The research was conducted using the transect method with 3 observation stations. The observed birds were then photographed and identified based on the field guide of MacKinnon. The results showed that 22 species 18 families were identified, including: Campephagidae, Vangidae, Sittidae, Pycnonotidae, Rhipiduridae, Nectariniidae, Eurylaimidae, Pellorneidae, Hirundinidae, Cisticolidae, Dicaeidae, Pittidae, Alcedinidae, Meropidae, Megalaimidae, Apodidae, Accipitridae and Cuculidae. Habitat conditions are strongly influenced by biotic and abiotic components to support the survival and diversity of birds on Mount Muria

Identification of Bird Species in Argowiloso Peak Mount Muria Ridlo, Muhammad; Setyawati, Siti Mukhlishoh; Hidayat, Saifullah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 1 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i1.13530

AbstractIndonesia is a country that has abundant flora and fauna. Mount Muria, Kudus Regency, Central Java has a high potential for bird biodiversity. The area which is a natural protected forest area, is very supportive for the existing bird habitat. However, this potential is still rarely known by the general public due to the lack of publications. The aims to find out and analyze the types of birds and to find out and analyze the condition of the bird habitat found in the Mount Muria area. The research was conducted using the transect method with 3 observation stations. The observed birds were then photographed and identified based on the field guide of MacKinnon. The results showed that 22 species 18 families were identified, including: Campephagidae, Vangidae, Sittidae, Pycnonotidae, Rhipiduridae, Nectariniidae, Eurylaimidae, Pellorneidae, Hirundinidae, Cisticolidae, Dicaeidae, Pittidae, Alcedinidae, Meropidae, Megalaimidae, Apodidae, Accipitridae and Cuculidae. Habitat conditions are strongly influenced by biotic and abiotic components to support the survival and diversity of birds on Mount Muria

Antagonism Trichoderma harzianum Rifai in Suppresing the Intensity of Antraknosa (Colletotrichum capcisi Sydow.) Disease Muliani, Yenny; Irmawatie, Lilis; Sukma, Siti Mariyam; Srimurni, Rafika Ratik; Adviany, Ida; Ustari, Debby; Milani, Mia Nurul
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 1 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i1.13546

Cayenne pepper (Capsicum frutescens L.) is a horticultural plant that has high economic value and contains nutrients that the body needs. Cayenne pepper is one of the leading national horticultural commodities, so it is necessary to increase the production of cayenne pepper appropriately. The obstacle that is often faced in the production of cayenne pepper is the disruption of the production of cayenne pepper caused by the attack of anthracnose disease. Anthracnose disease or fruit rot caused by the fungus Colletotrichum capcisi Sydow can result in yield losses ranging from 20-90%. Trichoderma harzianum Rifai. is a soil saprophytic fungus that naturally can be used as a biological agent, because it has antagonism against pathogens in the form of competition for space and nutrients, mycoparasites and antibiosis. The experimental results in the laboratory showed that the biological agent Trichoderma harzianum Rifai was able to suppress the development of anthracnose disease.

Antagonism Trichoderma harzianum Rifai in Suppresing the Intensity of Antraknosa (Colletotrichum capcisi Sydow.) Disease Muliani, Yenny; Irmawatie, Lilis; Sukma, Siti Mariyam; Srimurni, Rafika Ratik; Adviany, Ida; Ustari, Debby; Milani, Mia Nurul
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 1 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i1.13546

Cayenne pepper (Capsicum frutescens L.) is a horticultural plant that has high economic value and contains nutrients that the body needs. Cayenne pepper is one of the leading national horticultural commodities, so it is necessary to increase the production of cayenne pepper appropriately. The obstacle that is often faced in the production of cayenne pepper is the disruption of the production of cayenne pepper caused by the attack of anthracnose disease. Anthracnose disease or fruit rot caused by the fungus Colletotrichum capcisi Sydow can result in yield losses ranging from 20-90%. Trichoderma harzianum Rifai. is a soil saprophytic fungus that naturally can be used as a biological agent, because it has antagonism against pathogens in the form of competition for space and nutrients, mycoparasites and antibiosis. The experimental results in the laboratory showed that the biological agent Trichoderma harzianum Rifai was able to suppress the development of anthracnose disease.

Herpetofauna In the Ledok Ombo Natural Tourism Area, Malang District, East Java Hanifa, Berry Fakhry; Baihaqi, Ahmad Panji; Ahmad, Mujahidin; Kiptiyah, Kiptiyah; Hasyim, Muhammad Asmuni
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13566

The purpose of this study was to determine the diversity of herpetofauna in the Ledok Ombo Nature Tourism Area, Malang Regency. Herpetofauna specimens were collected using a Visual Encoumter Survey, Purposive Sampling at 07-11 PM. Specimens were collected 3 times one week apart in March 2022. The data was analyzed using Shannon Weiener Diversity, Pielou Evenness, Margalef Richness, and Simpson Dominance indices. Habitat Characteristic were recorded. Water-air temperature and air humidity were recorded. The results showed 9 species of amphibians (Chalcorana chalconota, Microhyla achatina, Duttaphrynus melanostictus, Phrynoidis aspera, Leptobrachium hasseltii, Odorrana hosii, Rhacoporus reinwardtii, Polypedates leucomystax, Occydozyga lima) and 9 species of reptiles (Cyrtodactylus marmoratus, Hemydactylus frenatus, Gonocephalus kuhlii, Broncochela jubata, Eutropis multifasciata, Pareas carinatus, Ahetulla prasina, Dendrelaphis pictus, Lycodon subcinctus). The index values of diversity, evenness, richness, and dominance are 1,591, 0,088, 3,250, 0,398 respectively. Habitat character affect the composition of herpetofauna. Physical factors value range for water temperature 23 - 24.55°C, air temperature 25.05 - 27.15°C, and air humidity 77.25 - 84.75%.

Herpetofauna In the Ledok Ombo Natural Tourism Area, Malang District, East Java Hanifa, Berry Fakhry; Baihaqi, Ahmad Panji; Ahmad, Mujahidin; Kiptiyah, Kiptiyah; Hasyim, Muhammad Asmuni
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13566

The purpose of this study was to determine the diversity of herpetofauna in the Ledok Ombo Nature Tourism Area, Malang Regency. Herpetofauna specimens were collected using a Visual Encoumter Survey, Purposive Sampling at 07-11 PM. Specimens were collected 3 times one week apart in March 2022. The data was analyzed using Shannon Weiener Diversity, Pielou Evenness, Margalef Richness, and Simpson Dominance indices. Habitat Characteristic were recorded. Water-air temperature and air humidity were recorded. The results showed 9 species of amphibians (Chalcorana chalconota, Microhyla achatina, Duttaphrynus melanostictus, Phrynoidis aspera, Leptobrachium hasseltii, Odorrana hosii, Rhacoporus reinwardtii, Polypedates leucomystax, Occydozyga lima) and 9 species of reptiles (Cyrtodactylus marmoratus, Hemydactylus frenatus, Gonocephalus kuhlii, Broncochela jubata, Eutropis multifasciata, Pareas carinatus, Ahetulla prasina, Dendrelaphis pictus, Lycodon subcinctus). The index values of diversity, evenness, richness, and dominance are 1,591, 0,088, 3,250, 0,398 respectively. Habitat character affect the composition of herpetofauna. Physical factors value range for water temperature 23 - 24.55°C, air temperature 25.05 - 27.15°C, and air humidity 77.25 - 84.75%.

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Contact Name
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Journal Mail Official
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Location
Kota semarang,

Jawa tengah

INDONESIA