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Menara Perkebunan
Published by Pusat Penelitian Kelapa Sawit
ISSN : 01259318     EISSN : 18583768     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Menara Perkebunan as a communication medium for research in estate crops published articles covering original research result on the pre- and post-harvest biotechnology of estate crops. The contents of the articles should be directed for solving the problems of production and/or processing of estate crops of smallholder, private plantations and state-owned estates, based on the three dedications of plantation. Analyses of innovative research methods and techniques in biotechnology, which are important for advancing agricultural research. Critical scientific reviews of research result in agricultural and estate biotechnology.
Arjuna Subject : -
Articles 541 Documents
 47   48   49   50   51 
Changes in chemical constituents and overall acceptability of papaya jam fortified with soya protein during storage Dimas Bayu PINANDOYO; Arisadi MASNAR
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.361

Abstract

Processed fruit products commonly contain very low protein due to the heat treatment during processing period. This article presents the researchresults concerning the increase of nutrition value of papaya jam fortified with soya protein concentrate. Papaya jam prepared with ratio 40:1, 40:2, 40:3, 40:4, and 40:5 (v/v) of papaya pulp and soya protein concentrate. The treatment of papaya jam and soya protein with the highest acceptability was then analyzed for the changes in chemical constituents and overall acceptability at monthly interval during three months of storage. The parameters that being analyzed were moisture content, total soluble solids (TSS), pH, total and reducing sugars, non-enzymatic browning, acidity, ascorbic acid, total carotenoid, total phenol, crude protein, and total plate count.  The results showed that fortified papaya jams prepared with papaya pulp and soy protein concentrate ratio at 40:1 had the highest acceptability. During storage time it revealed that moisture content, TSS, pH, total and reducing sugars, and non-enzymatic browning increased, while acidity, ascorbic acid, total carotenoids, phenols, total antioxidants and crude protein contents of jam decreased. Fortification of papaya jam with soya protein concentrate (40:1 v/v) increased crude protein content on average from 3.15% to 4.16%. Total plate count (TPC) indicated that no microbial contamination during 3 months storage period. It can be concluded that papaya jam fortified with soya protein concentrate remained acceptable during storage although the acceptability decreased over time.
Isolation and characterization of Dehydrin promoter region from sugarcane (Saccharum officinarum L.) Hayati MINARSIH; Sonny SUHANDONO; Anissa K FUADI; Tati KRISTIANTI; Riza A PUTRANTO; Deden SUKMAJDAYA; . SUSTIPRIJATNO
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.363

Abstract

The development of molecular biology techniques nowadays has enabled to engineer drought tolerant sugarcane by genetic engineering to accelerate the breeding program. Dehydrin (DHN) is known to have an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). While plant tissues are subjected to drought stress (dehydration), DHN protein is accumulated to high content throughout all vegetative or generative tissues. The research aimed to isolate and characterize the DHN promoter from sugarcane that can be used as transformation material in generating drought tolerant sugarcane. Specific primers for DHN promoter amplification were designed and DHN promoter region was successfully isolated by PCR cloning method. Two putative promoter sequences were identified namely Pr-1DHNSo and Pr-2DHNSo. In silicoanalyses were carried out and cis-regulatory elements motifs that play a role in adaptation on abiotic stress as well as biotic stress including ABRE, MBS, CGTCA-motif, TGACG-motif, GARE-motif, P-box TCA-element and Box-W1 were identified. The promoter Pr-1DHNSo was then cloned into pBI121 expression vector by Overlap Extention PCR (OE-PCR) for further characterization. Functional test of the promoter construct pBI- Pr-1DHNSo was conducted through Agrobacterium transformation into sugarcane calli. GUS assay and PCR analysis showed that the DHN promoter was transformed and expressed in the sugarcane calli.
Potential use of Claroideoglomus etunicatum to enrich signal grass (Brachiaria decumbens Stapf.) for silvopasture preparation Risa ROSITA; Rahayu WIDIASTUTI; Irdika MANSUR; Sarah Asih FAULINA
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.364

Abstract

Silvopasture system improvement in managing post-mining land resources has been done by searching for a quality grass. One of the selected grass species is signal grass (Brachiaria decumbens Stapf.). This research aimed to prepare signal grass through the inoculation of AMF Claroideoglomus etunicatum, as an effort to enrich its growth before being applied to post-mining soil. Research stages included the AMF inoculation on signal grass through spore culture and then transferred the colonized grass to the pot using sterile zeolite as a growth medium. The treatment on the first stage was without and with AMF inoculation (dose of 20 spores) on signal grass which was repeated for 12 times. Incubation in a spore culture was 4 weeks while incubation in a pot containing sterile zeolite medium was 8 weeks. Research data were analyzed using the Shapiro-Wilk’s normality test, Independent Sample T-test, and Pearson’s correlation test. Observation results showed that the inoculation of C. etunicatum on signal grass was significantly impact on the increase of plant height, stem diameter, number of leaves, number of tillers, shoot and root fresh weight, and shoot dry weight (p <0.05). Microscopic observation showed that there was AMF colonization on treated signal grass roots in the amount of 55 ± 0.06 % with number of spores was 252 ± 9.82 per 10 g zeolites, while AMF infection was not found in uninoculated signal grass. It is expected that by providing signal grass inoculated with AMF C. etunicatum would support its growth in post-mining land for Silvopasture system.
Bioconversion performance and development of black soldier fly (Hermetia illucens L.) on treated cocoa pod husk Ciptadi Achmad YUSUP; Haryo Tejo PRAKOSO; . SISWANTO; Deden Dewantara ERIS
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.365

Abstract

Indonesia is the third largest cocoa producer in the world, thus the number of cocoa pod husk (CPH) resulted from this activity is abundant. To handle this waste, farmer usually uses it directly as a feed source to small ruminants but this practice is less effective due to its low protein content and it also contains a substantial amount of lignin. Black Soldier Fly (BSF) (Hermetia illucens L.) (Diptera: Stratiomyidae) larvae are known as bioconversion agents that can be fed upon various organic substrates and they are also high protein source. The aim of this research was to evaluate the possibility of BSF grown on CPH based on their relative growth rate (RGR), efficiency of conversion of ingested food (ECI), waste reduction index (WRI), and development time. Body size of the imago from each treatment was also measured. Larvae were fed with fresh CPH (F), fresh blended CPH (B), composted CPH (C), mix of fresh CPH with food waste (F+FW) and mix of composted CPH with food waste (C+FW). Food waste served as a control. The results of this study show that the most ideal treatment that possible to be applied in cocoa plantation was C+FW treatmentwhich gave average prepupal fresh weight of 11.20 g/100 larvae with 18 days of development time. This treatment had the highest value of WRI and RGR among all treatments. Composted CPH that mixed with food waste treatment also had a shorter development time of BSF larvae.
Sintesis dan uji in vitro penghambatan nanokitosan-Cu terhadap pertumbuhan Fusarium oxysporum dan Colletotrichum capsici Sri WAHYUNI; Muhammad Alfian PRASETYO; Deden Dewantara ERIS; . PRIYONO; . SISWANTO
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.367

Abstract

Wilt and anthracnose are diseases on chili that cause substantial losses and even crop failure. Control of the diseases is generally carried out using chemical pesticides which are environmentally harmful. Therefore, the development of nanoparticles, such as nanochitosan-Cu, can be an environmentally friendly solution in controlling chili disease. The current technology in developing nanochitosan-Cu is green synthesis, which uses an effective reducing agent but non-toxic for plants and the environment. However, the process requires sonication, which is difficult to be adapted for scale-up production. This research aimed to synthesize and determine the formulation of nanochitosan –Cuusing magnetic stirrer method without sonication, and also to evaluate the antifungal ability of nanochitosan-Cu againstFusarium oxysporum and Colletotrichum capcisi causing wilt and anthracnose disease, respectively. Synthesis of nanochitosan-Cu was carried out at 50°C and 400 rpm of speed. Characterizations of nanochitosan-Cu were analyzed using SEM-EDX and PSA. In vitro antifungal activity test was carried out by food poisoning method.The results showed that the synthesis of nanochitosan-Cu using ionic gelation method can be carried out without sonication process, and produce round shape nanoparticles with 183.7 nm of diameter. The nanochitosan-Cu was effective against F. oxysporum and C. capsici, at a concentration of 100 ppm. It inhibited the growth of F. oxysporum and C. capsici by 100% and 92.38%, respectively.Layu dan antraknosa merupakan penyakit pada tanaman cabai yang menimbulkan kerugian besar bahkan gagal panen. Pengendalian penyakit tersebut umumnya dilakukan menggunakan pestisida kimia yang tidak ramah lingkungan. Untuk itu perlu dikembangkan biofungisida yang ramah lingkungan, salah satunya adalah nanopartikel kitosan-Cu. Teknologi pengembangan nanopartikel yang saat ini sedang berkembang adalah green synthesis, yaitu menggunakan reduktor yang efektif namun tidak toksik terhadap tanaman dan lingkungan. Proses sintesis ini membutuhkan sonikasi yang cukup sulit untuk diadaptasi pada skala produksi masal. Penelitian ini bertujuan untuk melakukan sintesis nanopartikel kitosan-Cu dengan metode magnetic stirrer tanpa sonikasi, serta mengevaluasi kemampuan antifungi terhadap Fusarium oxysporum dan Colletotrichum capcisi penyebab layu dan antraknosa. Sintesis nanokitosan-Cu dilakukan pada suhu 50°C dengan kecepatan 400 rpm. Karakterisasi nanokitosan-Cu dilakukan menggunakan SEM-EDX dan PSA. Uji aktivitas antifungi secara in vitro dilakukan menggunakan metode peracunan makanan. Hasil penelitian menunjukkan bahwa sintesis nanokitosan-Cu dengan metode gelasi ionik dapat dilakukan tanpa proses sonikasi dan menghasilkan nanopartikel berdiameter 183,7 nm dan berbentuk bulat. Nanokitosan-Cu yang dihasilkan efektif sebagai antifungi terhadap F. oxysporum dan C. capsici. Pada konsentrasi 100 ppm, nanokitosan-Cu mampu menghambat pertumbuhan F. oxysporum dan C. capsici masing-masing sebesar 100% dan 92,38%.
Application of organic fungicide in controlling basal stem rot disease for mature oil palm Happy WIDIASTUTI; Hayati MINARSIH; Djoko SANTOSO; Deden Dewantara ERIS; Galuh Wening PERMATASARI
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.368

Abstract

Ganoderma is a major pathogen in oil palm crops. Some efforts related to control the growth of Ganoderma have been conducted but still have not found an effective method. This study aims to develop an organic fungicide that has been tested in vitro, which effective in controlling the growth of Ganoderma. The optimization carried out includes the determination of the dose and time interval for application in 13-year-old mature oil palm. This organic fungicide application was the continuation of application during the previous year especially for the two best treatment which is application organic fungicide every week (1w) and every two weeks (2w). In this study, the treatments tested were three levels dose of organic fungicide (0, 1x and 2x) and two types of frequency application, i.e. every week (1w) and every other week (2w). The results showed that the best application of organic fungicides was every week application with twice doses (1w.2x), based on the parameters of the inhibition of Ganoderma’s fruiting body formation, primary and secondary root formation, the opening of spear leaves, and harvesting parameters. The application of organic fungicide able to recover the oil palm infected Ganoderma sp., with increasing the fresh fruit bunch and its weight around 70% and 78%, respectively.
SPAD-502 and atLEAF CHL PLUS values provide good estimation of the chlorophyll content for Hevea brasiliensis Müll. Arg. Leaves Andi Nur CAHYO; Rudi Hari MURTI; Eka Tarwaca Susila PUTRA; Tri Rini NURINGTYAS; Denis FABRE; Pascal MONTORO
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.369

Abstract

Measurement of chlorophyll content using destructive methods is not efficient due to a large number of samples, cost, and time needed. Estimationof chlorophyll content by nondestructive methods using handheld chlorophyll meter may be considered to improve efficiency. This research aimed to determine the formula to convert SPAD-502 and atLEAF CHL PLUS values (relative indicator of chlorophyll content) to estimated (absolute) rubber leaves chlorophyll content. Twenty leaves of rubber plant were measured using SPAD-502 and atLEAF CHL PLUS at the same time to determine SPAD-502 and atLEAF CHL PLUS values. The measured leaves were then collected to determine the chlorophyll content using a standard laboratory procedure. Regression and correlation analyses (among 3 methods) were conducted using SAS v.9 software. The results showed that between SPAD-502 and atLEAF CHL PLUS values were closely correlated, hence both of the devices can substitute each other to estimate rubber leaf chlorophyll content. In addition, the relationship between atLEAF CHL PLUS and SPAD-502 values with actual chlorophyll content of rubber clone SP 217, PB 260, GT1, and all clones (general) were significant with high coefficient of determination (R2) as well as low Root Mean Square Error (RMSE) and Coefficient of Variation (CV). Therefore, by using formula determined in this study, both atLEAF CHL PLUS and SPAD-502 can be suggested for accurate, fast, and non-destructive estimation of chlorophyll content of rubber plant leaf.
Application of bio-silicic acid to improve yield and fertilizer efficiency of paddy on tidal swamp land Donny Nugroho KALBUADI; Laksmita Prima SANTI; Didiek Hadjar GOENADI; Junita BARUS
Menara Perkebunan Vol. 88 No. 2 (2020): 88 (2), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i2.378

Abstract

The soils in tidal swamp land as new development area for rice in Indonesia have a considerably low available silica (Si) because very acid condition and no Si fertilization. Therefore, increasing the productivity of rice as Si accumulator plant, in tidal swamp land requires silica fertilizer. This research presented the effect of applications of silicon in the form of orthosilicic acid (H4SiO4) enriched with selected Sisolubilizing fungi, formulated as 4-gram tableted Si fertilizer (BioSilAc) on tidal swamp land soil to improve yield and reduce chemical fertilizer dosage for rice. Field experiment was conducted in Ketapang subdistrict, South Lampung by using a Randomized Block Design (RBD) with six treatments and four replicates. The treatments consisted of (ha-1 season-1): 100% NPK recommended dosage (P1); 100% NPK + 80 BioSilAc tablets (P2); 75% NPK + 80 BioSilAc tablets (P3); 75% NPK + 100 BioSilAc tablets (P4); 50% NPK + 120 BioSilAc tablets (P5); and control (P6). The results showed that P2 treatment increased the highest rice yield in dried harvested grain by 7.6% or equivalent to 500 kg ha-1 compared to P1 treatment. The highest fertilization efficiency can be achieved in P4 treatment which means the NPK recommended dosage can be reduced 25% by adding 100 BioSilAc tablets ha-1 season-1 indicated by insignificantly different productivity compared to that of P1. The Revenue/Cost (R/C) values showed that P2 treatment was economically feasible fertilization practices.
Structure-based virtual screening of bioherbicide candidates for weeds in sugarcane plantation using in silico approaches Galuh Wening PERMATASARI; Riza Arief PUTRANTO; Happy WIDIASTUTI
Menara Perkebunan Vol. 88 No. 2 (2020): 88 (2), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i2.379

Abstract

Weeds in sugarcane have negatively affected the sugar yield rate. Several approaches have been carried out to overcome the weeds, including the usage of diuron as synthetic herbicide. However, the long-term usage of diuron is known to have a negative effect leads to the production of 3,4- Dichloroaniline responsible for soil leach and bioaccumulation. Therefore, this study aimed to find a potential natural herbicide. By mimicking the diuron's mode of action which inhibits the process of photosynthesis through blocking the Photosystem II protein D1 (psbA) of the weeds, fourteen compounds as potential candidate bioherbicides were virtually docked by PyRx v0.9.5 software to the specific site. Three important species of the weeds were chosen including Eleusine indica, Praxelis clematidea, and Momordica charantia. The binding affinity score was further calculated and ranked to screen the top six compounds as bioherbicide candidates. Interaction of each complex and the biological activity prediction were then performed by Discovery Studio software and PASS server, respectively. Aurachin P, Aurachin A, and Cyanobacterin were placed in the top ranked compounds with high binding affinity score around -6 to -9 kcal mol-1 toward the psbA. The amino acid interaction involved in the complex shows 50-90% similar to the control, psbA and diuron complex. Besides, the biological activity prediction of Aurachin P, Aurachin A, and Cyanobacterin exhibits the terms related to the inhibition of photosynthesis process via enzymatic pathway. Thus, the active compounds might have inhibition action in the photosynthesis process and control  the weeds in sugarcane.
Pengaruh kitosan terhadap peningkatan level ekspresi WRKY17 dan WRKY53 tanaman Capsicum annuum cv. Laba pada kondisi kekeringan Muhammad Abdul AZIZ; Rizkita Rahmi ESYANTI; Fenny Martha DWIVANNY
Menara Perkebunan Vol. 88 No. 2 (2020): 88 (2), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i2.380

Abstract

Chitosan is known as the natural plant growth biostimulant and defense elicitor involving WRKY transcription factors in response to environmental stresses. However, either plant’s growth or defense responses against stress are different among the cultivars. In the previous study, chitosan and drought treatment combination on red chili plant cv. Lado resulted in the impeded growth performance followed by the down-regulated expression level of WRKY17. Hence, this study aimed to investigate the response of red chili plant from the different cultivar towards the combination of chitosan and drought treatments. At the onset of the generative phase, plants were subjected to the drought treatment with and without 1 mg mL-1 of chitosan application. Observation of plant growth performance was carried out by measuring plant height, the number of leaves, the conversion percentage of flowers developed to fruits, and the number of harvested fruits, while the molecular parameter was indicated by the analyses of WRKY17 and WRKY53 expression levels. In line with the previous study using cv. Lado, the combination of 1 mg mL-1 of chitosan and drought treatment significantly reduced the plant productivity observed in the number of fruits, followed by the plant height and the number of leaves. However, the expression level of WRKY17 and WRKY53 experienced 10 and 22-fold higher than controls, respectively. This is different from the previous study which showed the reduction of WRKY17 expression level. According to the study, it can be implied that the combination of chitosan and drought treatments on red chili plant cv. Laba could reduce plant growth performance, but increased genetics indicator towards plant’s defense system against stress indicated by the escalation of WRKY17 and WRKY53 expression level. Furthermore, the individual chitosan application are potential to increase the productivity of red chili plant cv. Laba. 

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