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Scripta Biologica

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Published by Universitas Jenderal Soedirman

ISSN : - EISSN : 23553138 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.

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Articles 176 Documents

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PERKEMBANGAN POST-LARVA IKAN NILEM Osteochilus hasselti C.V. DENGAN POLA PEMBERIAN PAKAN BERBEDA Didi Humaedi Yusuf; Gratiana E Wijayanti; Sugiharto Sugiharto
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Failure of adaptation in feed utilization can result in larval mortality. Post-larval stage is a critical period of fish development in which transition from internal feeding to external feeding occurs. This study aimed to determine the growth and survival of hard-lipped barb (Osteochilus hasselti C.V.) post-larvae fed with different feeding patterns. The experiment was conducted on an experimental basis with a completely randomized design (CRD). The treatments consisted of (P1) 100% Spirulina sp., (P2) 75% Spirulina sp. + 25% pellet powder, (P3) 50% Spirulina sp. + 50% powder pellet, (P4) 25% +75% sp Spirulina pellet powder, and (P5) 100% powder pellets. Day 1 post-hatching larvae were reared in aquaria with density of 17 fish / L for 8 weeks. The larvae were fed as much as 5% of the total weight of biomass. Post-larval development was evaluated by observing the development of the larvae morphology, the body length (every week), the biomass weight (every 2 weeks), and calculating their survival rate at week 8. Morphological data were analyzed descriptively. Data on body length, biomass weight and survival were analyzed using two-way ANOVA followed by LSD test. The results showed that the feeding pattern significantly affected the development of post-larvae (p<0.05). The most advance post-larval development was obtained from the post-larvae fed with 100% pellet powder. In this group, morphological characters of post-larvae in the first week were the mouth start opening, the skin was pigmented, and gall bladder in bi-lobes shaped, caudal fin and dorsal fins were differentiated. Completion of fins development was achieved by the third week when the post-larvae have adult morphological character. The highest body length and biomass weight of the post-larvae was obtained in the group fed with100% of pellets and the highest survival rate was found in the group fed with combination of 50% Spirulina sp. + 50% pellet powder (P3).

APPLICATION OF PROTEASE ENZYMES IN PROTEIN UTILIZATION AT GROWTH OF WHITE SNAPPER FISH (Lates calcalifer) Fitria Aditama; Johannes Hutabarat; Subandiyono Subandiyono
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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White Kakap (Lates calcalifer) is a superior commodity and has a high nutritional value. Growth factors are hampered by the ineffectiveness of the digestive process. Protease is an enzyme capable of hydrolyzing peptide bonds in proteins. Feed is a factor that predominantly affects the growth of fish because feed serves as a supplier of energy to support growth. Protein digestion in aquatic animals requires proteases as a catalyst. Enzymes that play an important role in the process of protein digestion are proteases. The purpose of this study is to examine the effect of adding different protease enzymes to artificial feed on the efficiency of protein utilization in the growth of white snapper (Lates calcalifer). The results of this study are expected to provide information about the importance of protease enzyme application in artificial feed in increasing protein hydrolysis, so that protein can be easily utilized by the body of white snapper (Lates calcalifer). The cultivars tested were white snapper (Lates calcalifer). The cultivation comes from intensively cultivated Lampung with an average weight of 6.5 ± 0.44 g / tail with a length ranging from 4-5 cm, as many as 75 heads. The research method is experimental laboratories using Completely Randomized Design (RAL). The tested feed was in the form of artificial feed with protein content of 40% and different protease dosage. The tested treatment was A feed (0.0% Protease enzyme from artificial feed weight), B (0.05% Protease enzyme from artificial feed weight), C (0.1% Protease enzyme from artificial feed weight), D (0 , 15% Protease enzyme from artificial feed weight), E (0.2% Protease enzyme from artificial feed weight). The ingredients of white snapper fish feed consist of fish meal, corn flour, soybean meal, shrimp flour, fish oil, corn oil, CMC / wheat flour, minerals and vitamins. Research data obtained for 40 days using analysis of variance (anova). If there is a real effect on the treatment then Duncan multiple area test is done. The result of this research indicated that the giving of protease 0,2% in artificial feed with protein 40% able to produce feed consumed Kakap Putih 43,43 ± 0,13 g, FCR 0,35 ± 0,002, Feed Pureness equal to 67,9 ± 1.23%, Protein Digestibility 98.25 ± 0.03%, Protein Retention 68.8 ± 0.23%, PER 7.97 ± 0.04, Fat Retention 0.6 ± 0.005%, E / P 4, 6 cal / g, thus giving effect on Specific Growth (SGR) 6.032 ± 0.17% and Length 8.3 ± 0.2 cm.

KEANEKARAGAMAN KEPITING PADA EKOSISTEM MANGROVE DI PERAIRAN NATUNA, KEPULAUAN RIAU Ernawati Widyastuti
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.702

ABSTRACTThis study aims to determine the diversity of crabs in the mangrove ecosystems in Natuna waters, Riau Islands. The study was conducted on 23 October to 5 November 2014. The crabs were collected from eight research stations using random method, by putting a transect quadrant of 1 x 1 m2 on an area of 10 x 10 m2 area at each mangrove observation station. Results obtained 274 individuals, from 21 species, 11 genera and 8 families. Crabs of the family Sesarmidae most diverse with 8 specie: Clistocoeloma sp., Nanosesarma edamensis, Parasesarma leptosoma, Parasesarma sp1., Perisesarma cf. brevicristatum, Perisesarma semperi, Perisesarma sp1. and Sarmatium crassum. Based on quantitative analysis, the index value of diversity ranged from 0.932 – 2.028, with the highest value obtained at Cemaga Utara (St. NTNM02). The eveness index in the range of 0.579 - 0.881 with the highest eveness at Cemaga Selatan (St. NTNM01).

KAJIAN ETNOBOTANI TUMBUHAN SEBAGAI BAHAN TAMBAHAN PANGAN SECARA TRADISIONAL OLEH MASYARAKAT DI KECAMATAN PEKUNCEN KABUPATEN BANYUMAS Ari Apriliani; Sukarsa Sukarsa; Hexa Apriliana Hidayah
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

People can’t detached from plants in fulfilling their needs, such as plant as food additives. This study aimed to know the types and parts of plants as food additives, and its benefits, as well as its utilization by people of Pekuncen District Banyumas Regency. This research used survey method with purposive random sampling and semi-structured interviews. Data were analyzed descriptively. The results of this study indicated 34 species of 19 familia plants used as food additives. The part of plants used as food additives were tubers, rhizomes, seeds, stems, leaves and fruits. Food additive plants were utilized as flavor enhancer, natural dye, preservatives and acid flavor. The plants were utilized by cooking, cuting, crushing, shredding, grinding, "dikeprek", roasting, boiling, and marinating.

PENGARUH INTENSITAS CAHAYA TERHADAP PERTUMBUHAN DAN KANDUNGAN KAROTENOID Dunaliella sp. PADA MEDIA EKSTRAK DAUN LAMTORO Leucaena leucocephala Doni Putra Pradana; Berta Putri; Siti Hudaidah
Scripta Biologica Vol 4, No 4 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Dunaliella sp. is a green microalga commonly used as a live feed in the hatchery, especially in marine aquaculture. This microalga is capable of producing carotenoids and accumulate a significant amount of β-carotene under stressful conditions, for example, high light intensity during the culture process. The objective of this study was to determine the effect of light intensity on the growth of Dunaliella sp. culture and it carotenoid content. The Dunaliella sp was cultivated for eight days at the Aquaculture Laboratory, Department of Fisheries and Marine Science, Faculty of Agriculture, University of Lampung. The study used a completely randomized design with three different light intensity as treatment, i.e., (A) 2500 lux, (B) 3500 lux, and (C) 4500 lux. All treatments were given three replicates, and the position of culture bottles was randomized with the assumption that all sample units received the equal amount of light intensity. The results showed the Dunaliella sp culture illuminated with light intensity higher than 2500 lux have higher carotenoid content.

ANALISIS BIOMASA DAN CADANGAN KARBON PADA BERBAGAI UMUR TEGAKAN DAMAR (Agathis dammara (Lamb.) Rich.) DI KPH BANYUMAS TIMUR Zinatul Uthbah; Eming Sudiana; Edy Yani
Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Increased carbon dioxide in the atmosphere causes global climate change seriously. Forests serve as an important asset that can absorb and store carbon in the form of biomass. One type of potential forest as a carbon sink is forests resin. The amount of carbon stored by standing very dynamic and varies according to the age of its standing. Therefore, studies will be needed to determine the effect of age on biomass and carbon stocks stands resin, determine the relationship between age and standing biomass and carbon stocks resin, and knowing the optimum resin stand age in storing biomass and carbon stocks. This research was conducted at the stands of resin RPH Karang Gandul, KPH Banyumas Timur for four weeks in May 2016. The method used is a survey with a sampling technique using cluster random sampling. Stands resin used in the study were classified into five age groups with 5 replicates. Data were analyzed using ANOVA with an error rate of 5% and continued with LSD for further test results were significant and regression analysis to determine the relationship of age with biomass and carbon stocks stands resin. The results showed that the age effect on biomass and carbon stocks stands resin, the relationship formed between the age of stand with biomass and carbon stocks are quadratic, and age optimum in storing biomass and carbon stocks is 35 years.

KEANEKARAGAMAN MIKROALGA DI SUNGAI KATI LUBUKLINGGAU Harmoko Harmoko; Sepriyaningsih Sepriyaningsih
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

We conducted an initial survey of the microalgal diversity of Kati River located in the City of Lubuklinggau, South Sumatra Province, on March to May 2017. This study aimed to determine the microalgal diversity of Kati River as a baseline data. This study found 47 microalgal species belonging to 42 genera and 27 orders. We found the member of Chlorophyceae and Bacillariophyceae were the most abundant microalgae. We also compared the diversity of Kati River to two other rivers in Lubuklinggai, Mesat River, and Kalingi River. The microalgal diversity of Kati River is higher than Mesat River but lower if compared to Kalingi River. The physicochemical factors of Kati River waters were also measured, the average water temperature was 27°C, average pH was 6.7, and average water transparency was 57 cm. This study found that those environmental factors influenced microalgal diversity in Lubuklinggau rivers, Kati River, as well as Mesat River and Kalingi Rivers, have indicated the same results.

ISOLASI DAN KARAKTERISASI JAMUR LIGNINOLITIK SERTA PERBANDINGAN KEMAMPUANNYA DALAM BIODELIGNIFIKASI Putri Elvira Valencia; Vincentia Irene Meitiniarti
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Fungi can survive in various environments with different media including wood. Lignin in timber is hard to be degraded and hydrolyzed efficiently because of its polymer form, composite and complex structure. Ligninolytic fungi produce an extracellular enzyme to withstand with toxic or mutagenic chemicals exposure and known to degrade different types of pollutant compounds. Lignin decomposers were also known to play a significant role in the pulping process of paper mills, used in waste treatment such as textile and hydrocarbon wastes. This study was conducted to obtain fungal isolates that have delignification capability and to compare the ability of fungal isolates in degrading lignin. Isolates were from rotten wood and soil using selective lignin medium with tannic acid as sole C source. This study characterized the isolates by their morphology and identified them using Morphology and Taxonomy of Fungi book by Bessey (1950). The ligninolytic capability comparison was conducted by measuring the transparent zone formed on selective lignin media. This research found 14 isolates of fungi and all of them had the ligninolytic capability. Aspergillus niger isolate has the highest ligninolytic capability by producing 6.45 cm clear zone diameter on the 7th day of incubation. Aureobasidium sp. has the smallest clear zone diameter of 1.9 cm within the same period.

KEANEKARAGAMAN MIKROALGA DITINJAU DARI FAKTOR FISIKA DAN KIMIA DI SUNGAI LOK ULO KABUPATEN KEBUMEN, JAWA TENGAH Bagas Prakoso; Tititk Tri Wahyuni
Scripta Biologica Vol 6, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Microalgae diversity research in the Lok Ulo River in Kebumen Regency, Central Java was conducted in September 2018. This study aimed to determine the diversity of microalgae in the Lok Ulo river. This research was conducted by field survey method. Determination of water sampling was chosen from 3 stations based on considerations to represent waters including: Station I (upstream part of the river), station II (middle part of the river) and station III (downstream part of the river). Each of the three water samples is taken. Water samples are taken to environmental laboratories for physical, chemical and biological analysis. The results showed that microalgae species found in the upstream, middle and downstream of the Lok Ulo river numbered 9 species, namely Oscillatoria sp., Cymbellahelvetica, Cymbellatumida, Cymbellanaviculiformis, Diatomaelongatum, Fragilaria sp, Synedraaccus, Synedraaffanis, Tabellaria sp. The relationship between TDS on microalgae diversity is shown through linear equations Y = 2.404-0.00014X. Based on these equations, TDS (X) in the Lok Ulo river is able to influence the diversity of microalgae with TDS is negative, every increase of 0.1 mg / l, TDS will reduce diversity by 0.00014 microalgae. The magnitude of the effect of TDS on the diversity of microalgae is indicated by the value of the correlation coefficient (r) of 0.972 with a significance of 0.000 there is a TDS relationship with diversity. The closeness of the relationship between TDS and the diversity of microalgae is indicated by the coefficient of determination (R2) of 94.5%. This means that the magnitude of the diversity of microalgae is influenced by TDS with closeness of 94.5.

KUANTITAS ANAKAN KULTUR SEMUT RANGRANG, Oecophylla smaragdina, SECARA ARTIFISIAL DENGAN MENGGUNAKAN BEBERAPA JENIS PAKAN BERBEDA Lintang Dianing Ratri; Edi Basuki; Darsono Darsono
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Oecophylla smaragdina or weaver ants is social insect that has an important role in the ecosystem. One of its main function is as a biological control agent in agriculture. The use of weaver ants in an effort to develop a biocontrol continues and increases, this has caused the significant decrease of the natural population of O. smaragdina. Therefore, the weaver ants rearing is needed to maintain its existence in natural habitat without excessive exploitation. The purpose of this study were to determine the effect of different types of feed i.e. snails, earthworms, and okara (tofu by-product) in the production of weaver ants kroto; and to determine the different of treatments of feeds provided to the production quantity of weaver ants kroto. The experimental method used in this study with a completely randomized design. Treatment was given by different protein sources feeding i.e. snails (Pomacea canaliculata), earthworm (Lumbricus rubellus), and okara. Five replicates were appliead for each treatment. Earthworms fed ants produced highest number of kroto with total of 1,030 individuals and weight 87.4 gram in total. Snails fed ants produced 866 individuals and weight 73.1 gram. Okara fed ants produced 543 individuals and weight 45.9 gram.

Page 12 of 18 | Total Record : 176

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